Research communications in chemical pathology and pharmacology最新文献_第4页

Immunization with synthetic peptide segments of a sperm protein impair fertility in rats. 用精子蛋白合成肽段免疫可损害大鼠的生育能力。

Research communications in chemical pathology and pharmacology Pub Date : 1994-04-01

G R Vanage, Y K Jaiswal, Y A Lu, J P Tam, L F Wang, S S Koide

{"title":"Immunization with synthetic peptide segments of a sperm protein impair fertility in rats.","authors":"G R Vanage, Y K Jaiswal, Y A Lu, J P Tam, L F Wang, S S Koide","doi":"","DOIUrl":"","url":null,"abstract":"The nucleotide sequence of the cDNA encoding a sperm protein (rSMP-B) was determined in a previous study. Two peptide segments corresponding to the extracellular domain of the deduced sperm polypeptide were synthesized as multiple antigen peptide (MAP) and designated as rSMP-229 and rSMP-230. Polyclonal antibodies were raised against the two MAPs. Sera obtained from rabbits immunized with rSMP-230 interacted with human and rabbit sperm membrane proteins with estimated molecular sizes of 72 and 20.1 kD, respectively. Adult female and male rats were immunized with the MAPs and their fertilities determined. Immunization of female rats with rSMP-229 and rSMP-230 induced infertility in 25% and 83% of the treated animals, respectively. All male rats immunized with rSMP-229 remained fertile; whereas animals immunized with rSMP-230 did not mate with normal cycling female rats. Three impotent male rats were found to regain their mating potency 45 days after the last booster injection. These findings demonstrated that immunization with rSMP-230 induced a reversible impotency in male rats. Serum testosterone and LH levels were reduced in rSMP-230-immunized male rats and were elevated in rSMP-229-immunized animals. Histopathological examination of sections of testes from male rats immunized with rSMP-230 showed impairment of spermatogenesis and sloughing of germ cells into the lumen of the seminiferous tubules. The testes of male rats immunized with rSMP-229 showed normal morphology and active spermatogenesis with scattered foci of nodular hyperplasia of Leydig cells in the interstitial areas. In conclusion, immunization with synthetic peptide segments corresponding to different domains of a deduced sperm protein induced infertility in a significant number of female rats and transient impotency in male rats.","PeriodicalId":21140,"journal":{"name":"Research communications in chemical pathology and pharmacology","volume":"84 1","pages":"3-15"},"PeriodicalIF":0.0,"publicationDate":"1994-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19035659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soluble CD14 (sCD14) levels in patients with multiple organ failure (MOF). 多器官衰竭(MOF)患者的可溶性CD14 (sCD14)水平。

Research communications in chemical pathology and pharmacology Pub Date : 1994-04-01

S Endo, K Inada, T Kasai, T Takakuwa, H Nakae, M Kikuchi, H Yamashita, M Yoshida

引用次数: 0

Tissue lipid peroxidation may be triggered by increased formation of bilirubin in vivo. 体内胆红素形成增加可能引发组织脂质过氧化。

Research communications in chemical pathology and pharmacology Pub Date : 1994-04-01

R Olinescu, R Alexandrescu, S A Hulea, F A Kummerow

引用次数: 0

Enzymatic reduction of xenobiotic alpha,beta-unsaturated ketones: formation of allyl alcohol metabolites from shogaol and dehydroparadol. 异种α、β -不饱和酮的酶还原:由shogaol和dehydroparadol生成烯丙醇代谢物。

Research communications in chemical pathology and pharmacology Pub Date : 1994-04-01

Y J Surh, S S Lee

引用次数: 0

Influence of stevioside on hepatic glycogen levels in fasted rats. 甜菊苷对禁食大鼠肝糖原水平的影响。

Research communications in chemical pathology and pharmacology Pub Date : 1994-04-01

M O Hübler, A Bracht, A M Kelmer-Bracht

引用次数: 0

Effects of flumazenil (Ro15-1788) on blood glucose and serum lipids in normoglycemic and normolipidemic rats. 氟马西尼(Ro15-1788)对正常血糖和正常血脂大鼠血糖和血脂的影响。

Research communications in chemical pathology and pharmacology Pub Date : 1994-04-01

J Horák, B Cuparencu

引用次数: 0

FK 506 (Tacrolimus) metabolism by rat liver microsomes and its inhibition by other drugs. 大鼠肝微粒体对fk506(他克莫司)的代谢及其对其他药物的抑制作用。

Research communications in chemical pathology and pharmacology Pub Date : 1994-04-01

T N Prasad, D D Stiff, N Subbotina, M A Zemaitis, G J Burckart, T E Starzl, R Venkataramanan

{"title":"FK 506 (Tacrolimus) metabolism by rat liver microsomes and its inhibition by other drugs.","authors":"T N Prasad, D D Stiff, N Subbotina, M A Zemaitis, G J Burckart, T E Starzl, R Venkataramanan","doi":"","DOIUrl":"","url":null,"abstract":"The in vitro metabolism of FK 506 and its inhibition by other drugs was studied with hepatic microsomes from rats pre-treated with dexamethasone, a selective cytochrome P-450 IIIA inducer. Nonspecific inhibitors of cytochrome P-450, such as ketoconazole, itraconazole, fluconazole and SKF 525 A, and most of the cytochrome P-450 IIIA specific substrates used in this study significantly inhibited FK 506 metabolism. Although cyclosporine is a known substrate of cytochrome P-450 IIIA, it had no effect on FK 506 metabolism. Cytochrome P-450 II substrates had minimal but significant effect on FK 506 metabolism. This data supports our earlier observations that FK 506 metabolism is mediated predominantly by the steroid inducible cytochrome P-450 IIIA enzyme subfamily. The results of this study indicate that in transplant patients there is a potential for an interaction of FK 506 with other drugs that are metabolized by the cytochrome P-450 IIIA subfamily or those that alter the activity of cytochrome P-450 IIIA subfamily. Careful monitoring and FK 506 dosing adjustment may be necessary to maintain therapeutic concentration and minimize toxicity in patients receiving this agent.","PeriodicalId":21140,"journal":{"name":"Research communications in chemical pathology and pharmacology","volume":"84 1","pages":"35-46"},"PeriodicalIF":0.0,"publicationDate":"1994-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18526329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selected tissue distribution of liposomal methylprednisolone in rats. 甲基强的松龙脂质体在大鼠体内的组织分布。

Research communications in chemical pathology and pharmacology Pub Date : 1994-04-01

E V Mishina, W J Jusko

{"title":"Selected tissue distribution of liposomal methylprednisolone in rats.","authors":"E V Mishina, W J Jusko","doi":"","DOIUrl":"","url":null,"abstract":"The distribution of methylprednisolone (MPL) in various rat tissues following 2 mg/kg IV bolus doses of liposomal drug (L-MPL) and drug in solution was investigated. Animals were sacrificed at selected times post-dosing until 120 h. Liver, spleen, thymus, heart, lungs, muscle, kidney and brain were excised and the concentrations of MPL were measured using HPLC after homogenizing organs in buffer. The incorporation of MPL in liposomes did not alter the uptake of drug by heart, lung and muscle. Drug concentrations in brain were undetectable. In the kidney the MPL concentrations after 1 h were higher for liposomal drug and not detectable at later time points. Tissue to plasma partition coefficients were close to unity in lung, heart, and muscle at 1-2 h after the dose of drug in solution and increased by 7.5 times for spleen and 6 times for thymus after the dose of L-MPL. There were no significant differences between the weights of organs expressed in percent of body weight. These results demonstrate that, while the sequestration of L-MPL by lymphatic tissues occurred, the uptake of drug by the other tissues did not increase. This may be beneficial for preferential targeting of the immune system.","PeriodicalId":21140,"journal":{"name":"Research communications in chemical pathology and pharmacology","volume":"84 1","pages":"47-52"},"PeriodicalIF":0.0,"publicationDate":"1994-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19035660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of the non-radioisotopic carbonyl metalloimmunoassay (CMIA) to diphenylhydantoin. 非放射性同位素羰基金属免疫分析法(CMIA)在二苯基苯妥英中的应用。

Research communications in chemical pathology and pharmacology Pub Date : 1994-04-01

A Varenne, A Vessières, P Brossier, G Jaouen

{"title":"Application of the non-radioisotopic carbonyl metalloimmunoassay (CMIA) to diphenylhydantoin.","authors":"A Varenne, A Vessières, P Brossier, G Jaouen","doi":"","DOIUrl":"","url":null,"abstract":"As part of our ongoing work to develop the new non-isotopic assay method carbonyl metalloimmunoassay (CMIA), whose efficacy has already been proven in the laboratory for phenobarbital and cortisol, we here present the steps involved in establishing CMIA of 5,5 diphenylhydantoin (DPH), one of the most commonly used antiepileptic medications. First, anti-DPH antibodies were obtained by injection of the immunogen DPH-3-valerate-BSA into rabbits. The titer value and specificity of these antibodies were examined by RIA using [14C]-DPH as tracer, and an antibody batch selected for its high titer value and good specificity for metabolites of DPH and other antiepileptic drugs. Next the organometallic complex Cr(CO)3-DPH, chosen as the CMIA tracer, was synthesized and shown to conserve a high recognition value for anti-DPH antibodies (CR = 200%). Isopropyl ether was selected as the best organic solvent for use in separating the free and bound fractions of the tracer. Employing the Cr(CO)3-DPH complex as tracer and FT-IR spectroscopy as the detection method, we were able to obtain a titration curve by CMIA using an amount of tracer identical to that used in RIA. The titer value obtained in CMIA is approximately twice that obtained by RIA. These results demonstrate the feasibility of DPH assay by the CMIA method.","PeriodicalId":21140,"journal":{"name":"Research communications in chemical pathology and pharmacology","volume":"84 1","pages":"81-92"},"PeriodicalIF":0.0,"publicationDate":"1994-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19035589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyploid accumulation associated with hyper-phosphorylation of histone H1 in hepatocytes of LEC mutant rat. LEC突变大鼠肝细胞中组蛋白H1超磷酸化与多倍体积累相关。

Research communications in chemical pathology and pharmacology Pub Date : 1994-04-01

K Sogawa, T Yamada, J K Kim, M Nishioka, K Matsumoto

引用次数: 0