Research communications in chemical pathology and pharmacology最新文献

PAF acetylhydrolase and arachidonic acid metabolite levels in patients with sepsis. 脓毒症患者PAF乙酰水解酶和花生四烯酸代谢物水平。

Research communications in chemical pathology and pharmacology Pub Date : 1994-06-01 DOI: 10.1097/00024382-199505000-00185

T. Takakuwa, S. Endo, H. Nakae, M. Kikichi, K. Inada, M. Yoshida

引用次数: 13

Enhanced sensitivity of mdx mice to intramuscular injection of compound 48/80. 增强mdx小鼠肌肉注射复方48/80的敏感性。

Research communications in chemical pathology and pharmacology Pub Date : 1994-06-01

J A Granchelli, M S Hudecki, C M Pollina

{"title":"Enhanced sensitivity of mdx mice to intramuscular injection of compound 48/80.","authors":"J A Granchelli, M S Hudecki, C M Pollina","doi":"","DOIUrl":"","url":null,"abstract":"Species-specific differences in the inflammatory response, specifically with regard to mast cells, have been proposed to explain the phenotypic variation among dystrophin-deficient humans, and mdx mice (Gorospe et al., 1994). To test this hypothesis we have intramuscularly injected a mast cell secretogogue into both dystrophin-negative mdx and dystrophin-positive normal mice. Mast cell activity was determined by measuring the activity of mast cell tryptase, while creatine kinase activity was used to determine the course of muscle damage in vivo. Area of damage around the injection site was measured at autopsy, and used as an indication of relative sensitivity to the secretogogue effect of compound 48/80. Mdx mice exhibited more damage in response to intramuscular injection than normal control mice. In addition, mdx mice showed a substantial increase in plasma tryptase activity, followed by a large increase in muscle creatine kinase activity. On the other hand, dystrophin-positive normal controls injected with 48/80 liberated little CK or tryptase activity. These results are consistent with the hypothesis that species-specific differences in mast cell activity, or sensitivity to mast cell products could account for the variation in pathology seen in dystrophin-deficient animals.","PeriodicalId":21140,"journal":{"name":"Research communications in chemical pathology and pharmacology","volume":"84 3","pages":"351-62"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18935434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elevation of ceruloplasmin activity involved in changes of hepatic metal concentration in primary biliary cirrhosis. 原发性胆汁性肝硬化中铜蓝蛋白活性升高与肝金属浓度变化有关。

Research communications in chemical pathology and pharmacology Pub Date : 1994-06-01

K Sogawa, T Yamada, Y Suzuki, T Masaki, S Watanabe, Y Uchida, K Arima, M Nishioka, K Matsumoto

引用次数: 0

Blood cytokine and complement levels in patients with sepsis. 脓毒症患者血液细胞因子和补体水平的变化。

Research communications in chemical pathology and pharmacology Pub Date : 1994-06-01

T Takakuwa, S Endo, H Nakae, M Kikuchi, N Baba, K Inada, M Yoshida

{"title":"Blood cytokine and complement levels in patients with sepsis.","authors":"T Takakuwa, S Endo, H Nakae, M Kikuchi, N Baba, K Inada, M Yoshida","doi":"","DOIUrl":"","url":null,"abstract":"We measured serum levels of endotoxin, cytokines, and eicosanoids and investigated their relationship to serum complement levels in patients with sepsis. Serum endotoxin (Et) levels (5.3 +/- 2.4 pg/ml) were within the normal range, but levels of tumor necrosis factor-alpha (TNF-alpha, 114 +/- 104.94 pg/ml), interleukin 6 (IL-6, 86.7 +/- 50.9 pg/ml), interleukin 8 (IL-8, 86.8 +/- 49.7 pg/ml), type-II phospholipase A2 (type II PLA2, 211.3 +/- 193.9 ng/ml), leukotriene B4 (LTB4, 88.7 +/- 27.2 pg/ml), thromboxane B2 (TXB2, 58.7 +/- 50.9 pg/ml) and 6-keto-prostaglandin F1 alpha (PGF1 alpha, 21.0 +/- 11.0 pg/ml) levels were above normal. Levels of C3a (1088.4 +/- 83.8.7 ng/ml) and C4a (1951.5 +/- 1697.8 ng/ml) were also above normal; C3 (66.0 +/- 25.6 mg/dl) and C4 (23.6 +/- 5.3 mg/dl) were within the normal range, and C5a was lower than the detectable limit in all but one of the subjects. Serum TNF-alpha was significantly correlated with C3a (p < 0.001). Serum IL-6 had a significant negative correlation with C3 (p = 0.002) and C4 (p = 0.010). Type II PLA2 was significantly correlated with C3a (p < 0.001). There were no significant correlations between serum Et or IL-8 and serum C3, C4, C3a or C4a. Our findings suggest that increased levels of TNF-alpha, IL-6, and Type II PLA/ in patients with sepsis contribute to activation of the complement system.","PeriodicalId":21140,"journal":{"name":"Research communications in chemical pathology and pharmacology","volume":"84 3","pages":"291-300"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18935577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bradykinin induces generation of reactive oxygen species in bovine aortic endothelial cells. 缓激肽诱导牛主动脉内皮细胞产生活性氧。

Research communications in chemical pathology and pharmacology Pub Date : 1994-06-01

S Shimizu, M Ishii, T Yamamoto, T Kawanishi, K Momose, Y Kuroiwa

{"title":"Bradykinin induces generation of reactive oxygen species in bovine aortic endothelial cells.","authors":"S Shimizu, M Ishii, T Yamamoto, T Kawanishi, K Momose, Y Kuroiwa","doi":"","DOIUrl":"","url":null,"abstract":"We investigated the effects of bradykinin on intracellular oxidative stress in bovine aortic endothelial cells using a hydroperoxide-sensitive fluorescent dye, 2',7'-dichlorofluorescein (DCFH), and a laser scanning confocal microscope. Bradykinin induced an immediate increase in intracellular Ca2+ concentration, and stimulated the oxidation of DCFH in cultured endothelial cells. This bradykinin-induced oxidation of DCFH was inhibited by pretreatment with N-(2-mercaptopropionyl)-glycine (MPG) and 1,3-dimethyl-thiourea (DMTU), scavengers of hydroxyl radical, and the removal of extracellular Ca2+ but was unaffected by NG-nitro-L-arginine or NG-monomethyl-L-arginine, both inhibitors of nitric oxide (NO) synthase. On the other hand, pretreatment with indomethacin and aspirin, inhibitors of cyclooxygenase, inhibited bradykinin-induced oxidation of DCFH. These findings suggest that bradykinin increases intracellular Ca2+ and stimulates the generation of hydroxyl radical-like reactive oxygen species (scavenged by MPG or DMTU) via the cyclooxygenase pathway but not via the reaction of NO and superoxide anion.","PeriodicalId":21140,"journal":{"name":"Research communications in chemical pathology and pharmacology","volume":"84 3","pages":"301-14"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18530938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Copper cytotoxicity impairs DNA synthesis but not protein phosphorylation upon growth stimulation in LEC mutant rat. 在LEC突变大鼠的生长刺激下，铜细胞毒性损害DNA合成，但不损害蛋白质磷酸化。

Research communications in chemical pathology and pharmacology Pub Date : 1994-06-01

J K Kim, T Yamada, K Matsumoto

引用次数: 0

Vasodilator effects of clonidine on the mesenteric arterial beds in normotensive and spontaneously hypertensive rats. 可乐定对正常血压和自发性高血压大鼠肠系膜动脉床的血管舒张作用。

Research communications in chemical pathology and pharmacology Pub Date : 1994-06-01

K Kamata, T Numazawa, Y Kasuya

引用次数: 0

Relationships between plasma levels of type-II phospholipase A2, PAF-acetylhydrolase, leukotriene B4, complements, endothelin-1, and thrombomodulin in patients with sepsis. 脓毒症患者血浆ii型磷脂酶A2、paf -乙酰水解酶、白三烯B4、补体、内皮素-1和血栓调节蛋白水平的关系

Research communications in chemical pathology and pharmacology Pub Date : 1994-06-01

T Takakuwa, S Endo, H Nakae, T Suzuki, K Inada, M Yoshida, M Ogawa, K Uchida

{"title":"Relationships between plasma levels of type-II phospholipase A2, PAF-acetylhydrolase, leukotriene B4, complements, endothelin-1, and thrombomodulin in patients with sepsis.","authors":"T Takakuwa, S Endo, H Nakae, T Suzuki, K Inada, M Yoshida, M Ogawa, K Uchida","doi":"","DOIUrl":"","url":null,"abstract":"We determined the plasma levels of type-II phospholipase A2 (type II PLA2), platelet-activating factor acetylhydrolase (PAFAH) leukotriene B4 (LTB4) and of several complements (C3a, C4a, and C5a), which are considered to be among the cytokines and eicosanoids involved in vascular endothelial disorders and that vary in concentration during sepsis. We investigated the relationship between those levels and those of ET-1 and TM levels in plasma. Plasma levels of type II PLA2, PAFAH, LTB4, C3a, C4a, ET-1, and TM at the time that sepsis was diagnosed in 30 patients were 218.3 +/- 179.9 ng/ml, 23.92 +/- 9.66 nmol/min/ml, 90.35 +/- 31.49 pg/ml, 838.73 +/- 2.30 pg/ml, 1951.46 +/- 1697.78 pg/ml, 6.98 +/- 4.08 pg/ml and 7.80 +/- 3.34 ng/ml, respectively. The C5a plasma level was below the limit of detection in all cases. There were significant correlations between type II PLA2 and ET-1 plasma levels (r = 0.39, p = 0.032) and C3a and ET-1 plasma levels (r = 0.60, p = 0.03). There were also significant correlations between type II PLA2 and TM levels in plasma (r = 0.76, p = 0.0017), PAFAH and TM plasma levels (r = 0.53, p = 0.037), LTB4 and TM plasma levels (r = 0.46, p = 0.016) and C4a and TM plasma levels (r = 0.58, p = 0.037). Results suggest that the elevation of type II PLA2, PAFAH, LTB4 and complement in plasma is involved in vascular endothelial disorders in patients with sepsis.","PeriodicalId":21140,"journal":{"name":"Research communications in chemical pathology and pharmacology","volume":"84 3","pages":"271-81"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18935574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasma levels of TNF-alpha, endothelin-1 and thrombomodulin in patients with sepsis. 脓毒症患者血浆中tnf - α、内皮素-1和血栓调节素水平的变化。

Research communications in chemical pathology and pharmacology Pub Date : 1994-06-01

T Takakuwa, S Endo, H Nakae, M Kikichi, T Suzuki, K Inada, M Yoshida

{"title":"Plasma levels of TNF-alpha, endothelin-1 and thrombomodulin in patients with sepsis.","authors":"T Takakuwa, S Endo, H Nakae, M Kikichi, T Suzuki, K Inada, M Yoshida","doi":"","DOIUrl":"","url":null,"abstract":"To investigate the roles of tumor necrosis factor-alpha (TNF-alpha), endothelin-1 (ET-1) and thrombomodulin (TM) in the plasma of patients with sepsis, plasma levels of endotoxin (Et), TNF-alpha, ET-1 and TM were determined in 30 such patients. Plasma levels of Et, TNF-alpha, ET-1 and TM at the time sepsis was diagnosed were 4.0 +/- 6.7 pg/ml, 98.5 +/- 92.1 pg/ml, 7.0 +/- 4.1 pg/ml and 7.8 +/- 3.3 ng/ml, respectively. There was no significant difference in the plasma Et level between the group of patients that survived (n = 13) or died (n = 17). Plasma levels of TNF-alpha, Et-1 and TM were significantly higher in the group that died than in the surviving group (TNF-alpha, p < 0.0001; ET-1, p = 0.028; TM, p = 0.0004). There were significant correlations between the plasma levels of TNF-alpha and ET-1, of TNF-alpha and TM, and of ET-1 and TM (r = 0.37, p < 0.046; r = 0.61, p = 0.0008; r = 0.63, p = 0.004), respectively. Results suggest that TNF-alpha is involved in the production of ET-1 and TM. Both of those substances appear to be involved in the morbidity of sepsis, and their plasma levels reflect its severity.","PeriodicalId":21140,"journal":{"name":"Research communications in chemical pathology and pharmacology","volume":"84 3","pages":"261-9"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18935573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PAF acetylhydrolase and arachidonic acid metabolite levels in patients with sepsis. 脓毒症患者PAF乙酰水解酶和花生四烯酸代谢物水平。

Research communications in chemical pathology and pharmacology Pub Date : 1994-06-01

T Takakuwa, S Endo, H Nakae, M Kikichi, K Inada, M Yoshida

引用次数: 0