Research communications in chemical pathology and pharmacology最新文献_第10页

Immunoreactive hepatocyte growth factor is present in tissue extracts from human breast cancer but not in conditioned medium of human breast cancer cell lines. 免疫反应性肝细胞生长因子存在于人乳腺癌组织提取物中，但不存在于人乳腺癌细胞系的条件培养基中。

Research communications in chemical pathology and pharmacology Pub Date : 1993-11-01

J Yamashita, M Ogawa, T Beppu

引用次数: 0

Antioxidant and pro-oxidant assay for a new drug GEPC: detected by ESR spectrometry and by protective effects on lipid peroxidation and biomolecule degradation. 新药物GEPC的抗氧化和促氧化分析:ESR光谱法检测及其对脂质过氧化和生物分子降解的保护作用。

Research communications in chemical pathology and pharmacology Pub Date : 1993-11-01

J Liu, A Mori, K Ogata

{"title":"Antioxidant and pro-oxidant assay for a new drug GEPC: detected by ESR spectrometry and by protective effects on lipid peroxidation and biomolecule degradation.","authors":"J Liu, A Mori, K Ogata","doi":"","DOIUrl":"","url":null,"abstract":"L-Ascorbic acid 2-(20 beta-11-oxo-olean-12-en-29-oic acid ethylester-3-beta-yl hydrogen phosphate) sodium salt (GEPC) is a newly synthesized compound representing a phosphate diester linkage of glycyrrhetic acid ethylester and ascorbic acid. In the present study, we found that GEPC effectively inhibited Fe(III)-ADP/NADPH-induced peroxidation of liver microsomes. The inhibitory effect was much greater than that of glycyrrhetic acid (GA), and contrasted with the stimulatory effect of ascorbic acid. An ESR study showed that GEPC appeared to have a great loss of the DPPH and superoxide radical scavenging effects of ascorbic acid. However, GEPC, like ascorbic acid, inhibited hydroxyl radicals generation in both Fe(II)-H2 O2 and Cr(VI)-H2 O2 systems. GEPC, unlike ascorbic acid, showed no pro-oxidant effect and acted as an effective iron-chelating agent in the ESR study or in the iron-induced deoxyribose and DNA degradation assays. The hydroxyl radical scavenging effect of GEPC was further demonstrated by its protective effect on the hydroxyl radical- induced degradation of certain biomolecules, i.e., carbohydrates, amino acids, and DNA. These results demonstrate that beside its protective effect on ascorbic acid autoxidation and increasing water solubility of GA, GEPC is also an antioxidant though not so powerful as ascorbic acid but more powerful than GA.","PeriodicalId":21140,"journal":{"name":"Research communications in chemical pathology and pharmacology","volume":"82 2","pages":"151-66"},"PeriodicalIF":0.0,"publicationDate":"1993-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19290660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvement of the energy status of hypoxic hepatocytes by calcium channel blockers. 钙通道阻滞剂对缺氧肝细胞能量状态的改善。

Research communications in chemical pathology and pharmacology Pub Date : 1993-11-01

M Brecht, C Brecht, H de Groot

{"title":"Improvement of the energy status of hypoxic hepatocytes by calcium channel blockers.","authors":"M Brecht, C Brecht, H de Groot","doi":"","DOIUrl":"","url":null,"abstract":"Isolated hepatocytes from rat liver in primary culture rapidly lost viability under hypoxic conditions. Hypoxic injury was significantly decreased by the calcium channel blockers nifedipine (5 microM) and diltiazem (10 microM). The concentrations of the inhibitors which afforded maximum protection also produced the maximum increase in the energy level of the hypoxic hepatocytes, as evidenced by their ATP, ADP, AMP, and total adenine nucleotide content and by their energy charge. The increased hypoxic energy level caused by these calcium channel blocking agents was not due to an increased rate of anaerobic glycolysis; nifedipine did not have any effect on lactate production while diltiazem slightly decreased its rate. During the first 2 h under hypoxic conditions the cytosolic Ca2+ concentration remained constant around 100 nM, subsequently increasing to 400 nM first slowly and later more rapidly. The calcium channel blockers delayed the Ca2+ increase by about 1 h but were without any effect on the rate of this increase. The results suggest that the well-known beneficial effects of calcium channel blockers on hypoxic liver injury are due in large measure to an improved energetic situation of the hepatocytes rather than to the increase in the cytosolic Ca2+ concentration being blocked.","PeriodicalId":21140,"journal":{"name":"Research communications in chemical pathology and pharmacology","volume":"82 2","pages":"185-98"},"PeriodicalIF":0.0,"publicationDate":"1993-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19290663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in hepatic copper distribution leading to hepatitis in LEC rats. LEC大鼠肝铜分布变化致肝炎的研究。

Research communications in chemical pathology and pharmacology Pub Date : 1993-11-01

K T Suzuki, S Kanno, S Misawa, Y Sumi

{"title":"Changes in hepatic copper distribution leading to hepatitis in LEC rats.","authors":"K T Suzuki, S Kanno, S Misawa, Y Sumi","doi":"","DOIUrl":"","url":null,"abstract":"Copper (Cu) accumulating in the liver of LEC (Long-Evans with a cinnamon-like coat color) rats due to a hereditary metabolic disorder is assumed to cause acute hepatitis with severe jaundice or chronic hepatitis leading to cancer. Changes in concentrations and distributions of Cu, zinc and iron in the liver of LEC rats were determined to find the relationship between the chemical forms and the toxicity. Female rats after delivery were used because of high susceptibility to acute hepatitis. They were divided into four stages according to the development of jaundice. Cu concentrations in the whole liver and the supernatant decreased with development of jaundice. Distribution profiles of Cu, zinc, iron and sulfur on a gel filtration column by HPLC-ICP showed that Cu in the liver supernatant was mostly bound to metallothionein (MT) before jaundice (stage 1), high molecular weight proteins and MT at the beginning of jaundice (stages 2 and 3), and then mostly to MT at severe jaundice (stage 4) though the concentration of Cu at this stage was decreased to about 50% of stage 1. The results suggest that Cu accumulating as MT in the liver is liberated drastically after exceeding the capacity of MT synthesis, and the liberated Cu causes acute hepatitis.","PeriodicalId":21140,"journal":{"name":"Research communications in chemical pathology and pharmacology","volume":"82 2","pages":"217-24"},"PeriodicalIF":0.0,"publicationDate":"1993-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19290664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon tetrachloride promoted malondialdehyde formation in liver microsomal and nuclear preparations from Sprague Dawley or Osborne Mendel male rats. 四氯化碳促进了Sprague Dawley或Osborne Mendel雄性大鼠肝微粒体和核制剂中丙二醛的形成。

Research communications in chemical pathology and pharmacology Pub Date : 1993-11-01

S L Fanelli, J A Castro

引用次数: 0

Changes in copper distribution in the plasma and kidneys of LEC rats following acute hepatitis. 急性肝炎后LEC大鼠血浆和肾脏铜分布的变化。

Research communications in chemical pathology and pharmacology Pub Date : 1993-11-01

K T Suzuki, S Kanno, S Misawa, Y Sumi

{"title":"Changes in copper distribution in the plasma and kidneys of LEC rats following acute hepatitis.","authors":"K T Suzuki, S Kanno, S Misawa, Y Sumi","doi":"","DOIUrl":"","url":null,"abstract":"Copper (Cu) accumulating bound to metallothionein (MT) in the liver of LEC (Long-Evans with cinnamon-like coat color) rats due to a hereditary metabolic disorder is assumed to lead to acute hepatitis with severe jaundice. The metal was shown to be present in the liver in a form not bound to MT at the beginning of hepatitis after first delivery and lactation. Following this change in the distribution of Cu from MT-bound to non-MT bound form in the liver, changes in the concentrations and distributions of Cu, zinc (Zn) and iron in the plasma and kidneys of LEC rats were also observed. Cu plasma distribution on a gel filtration column by HPLC-ICP revealed that the holo-form of ceruloplasmin (Cp) was present before hepatitis and increased with its development, indicating the availability of Cu for Cp by hepatitis. Cu-binding proteins migrating at the same retention times as those of hepatic Cu-MT and Cu,Zn-superoxide dismutase (SOD) were detected in plasma during hepatitis. Albumin was largely present in the form of nonmercaptoalbumin, reflecting that the bloodstream was under oxidative stress. A sudden increase in the concentration of Cu in the kidneys occurred with hepatitis, and the metal came to be distributed more to high molecular weight proteins with its development.","PeriodicalId":21140,"journal":{"name":"Research communications in chemical pathology and pharmacology","volume":"82 2","pages":"225-32"},"PeriodicalIF":0.0,"publicationDate":"1993-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19289692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of twenty-three drugs on the metabolism of FK506 by human liver microsomes. 23种药物对人肝微粒体代谢FK506的影响。

Research communications in chemical pathology and pharmacology Pub Date : 1993-11-01

K Iwasaki, H Matsuda, K Nagase, T Shiraga, Y Tokuma, K Uchida

引用次数: 0

Anxiolytic activity of melatonin in mice: involvement of benzodiazepine receptors. 褪黑素在小鼠中的抗焦虑活性:苯二氮卓类受体的参与。

Research communications in chemical pathology and pharmacology Pub Date : 1993-11-01

G Pierrefiche, R Zerbib, H Laborit

引用次数: 0

Active oxygen formation in alveolar macrophage and pulmonary tumorigenesis. 肺泡巨噬细胞活性氧形成与肺肿瘤发生。

Research communications in chemical pathology and pharmacology Pub Date : 1993-11-01

T Yano, T Ichikawa

引用次数: 0

Urinary proteoglycans after cadmium exposure. 镉暴露后的尿蛋白聚糖。

Research communications in chemical pathology and pharmacology Pub Date : 1993-11-01

H Savolainen

引用次数: 0