Reproduction, nutrition, development最新文献

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Importance of the signals provided by the buck for the success of the male effect in goats. 雄鹿提供的信号对山羊雄性效应成功的重要性。
Reproduction, nutrition, development Pub Date : 2006-07-01 Epub Date: 2006-07-07 DOI: 10.1051/rnd:2006020
J Alberto Delgadillo, J Alfredo Flores, Francisco G Véliz, Gerardo Duarte, Jesús Vielma, Horacio Hernandez, Ilda G Fernandez
{"title":"Importance of the signals provided by the buck for the success of the male effect in goats.","authors":"J Alberto Delgadillo,&nbsp;J Alfredo Flores,&nbsp;Francisco G Véliz,&nbsp;Gerardo Duarte,&nbsp;Jesús Vielma,&nbsp;Horacio Hernandez,&nbsp;Ilda G Fernandez","doi":"10.1051/rnd:2006020","DOIUrl":"https://doi.org/10.1051/rnd:2006020","url":null,"abstract":"<p><p>Under temperate and subtropical latitudes, ewes and goats display a reproductive seasonal pattern and their sexual activity during the anestrous period can be stimulated and synchronized by the introduction of males in the group, which is called the \"male effect\". The response of females to the male effect in the middle of the anestrous season is weak or absent. This failure may be due to the inability of the female to respond to males, as a result of a refractoriness of the female to the male stimulus. But, it may also be due to a low quality stimulus provided by the male which is, as the females, in seasonal rest. We tested this latter hypothesis in seasonally inactive goats kept under subtropical conditions by comparing the use of males with their sexual behavior stimulated or not by photoperiodic treatments. Treated males were able to induce estrous activity of females during the whole anestrus season. We have also determined that previous separation of the males and continuous contact during teasing are not absolute requisites when active bucks are used. While odor from the male and its sexual behavior play a primary role in inducing ovulation, vocalizations appear to facilitate the display of the does' estrous. It remains to be determined to which extent these conclusions apply under temperate latitudes and with more seasonal breeds.</p>","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"46 4","pages":"391-400"},"PeriodicalIF":0.0,"publicationDate":"2006-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2006020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26129691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 40
The effect of dietary protein on the amino acid supply and threonine metabolism in the pregnant rat. 饲粮蛋白质对妊娠大鼠氨基酸供应及苏氨酸代谢的影响。
Reproduction, nutrition, development Pub Date : 2006-05-01 Epub Date: 2006-05-30 DOI: 10.1051/rnd:2006015
William D Rees, Susan M Hay, Christos Antipatis
{"title":"The effect of dietary protein on the amino acid supply and threonine metabolism in the pregnant rat.","authors":"William D Rees,&nbsp;Susan M Hay,&nbsp;Christos Antipatis","doi":"10.1051/rnd:2006015","DOIUrl":"https://doi.org/10.1051/rnd:2006015","url":null,"abstract":"<p><p>To characterise the effects of dietary protein content on threonine metabolism during pregnancy, rats were fed diets containing 18% or 9% protein and then killed at different stages of gestation. Serum threonine concentrations fell significantly faster in the animals fed the diet containing 9% protein when compared to those fed the diet containing 18% protein. On day 4 of gestation the rate of threonine oxidation was higher in maternal liver homogenates prepared from the animals fed the diet containing 18% protein. The rate of threonine oxidation by liver homogenates fell as gestation proceeded in both diet groups. The activity of threonine dehydrogenase in the maternal liver was unaffected by dietary protein content at all stages of gestation. Serine-threonine dehydratase activity in homogenates of the maternal liver was transiently increased during the early stages of gestation in the animals fed high protein diets but was unchanged in the low protein groups. There was an increase in serine-threonine dehydratase activity in the kidney during the later stages of gestation but this was unaffected by the protein content of the maternal diet. These data show that the changes in free threonine concentrations cannot be accounted for through changes in the oxidation rate and suggest that some other factor influences the unusual metabolism of this amino acid during gestation.</p>","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"46 3","pages":"227-39"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2006015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26050938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Pharmacological profile of inhibition of the chloride channels activated by extracellular acid in cultured rat Sertoli cells. 细胞外酸对培养大鼠支持细胞中氯离子通道的抑制作用。
Reproduction, nutrition, development Pub Date : 2006-05-01 Epub Date: 2006-05-30 DOI: 10.1051/rnd:2006013
Céline Auzanneau, Caroline Norez, Sabrina Noël, Chantal Jougla, Frédéric Becq, Clarisse Vandebrouck
{"title":"Pharmacological profile of inhibition of the chloride channels activated by extracellular acid in cultured rat Sertoli cells.","authors":"Céline Auzanneau,&nbsp;Caroline Norez,&nbsp;Sabrina Noël,&nbsp;Chantal Jougla,&nbsp;Frédéric Becq,&nbsp;Clarisse Vandebrouck","doi":"10.1051/rnd:2006013","DOIUrl":"https://doi.org/10.1051/rnd:2006013","url":null,"abstract":"<p><p>Sertoli cells from mammalian testis are key cells involved in the development and maintenance of stem cell spermatogonia as well as in the secretion of a Cl(-) and K(+)-rich fluid into the lumen of seminiferous tubules. The pharmacology and contribution of Cl(-) channels to the physiology of Sertoli cells were investigated using whole-cell patch clamp and iodide efflux experiments applied to cultured rat Sertoli cells. We characterized an outwardly rectifying Cl(-) current stimulated by various acid species including the physiologically relevant lactic acid. Using the iodide efflux technique, the pharmacological properties of this Cl(-) current, noted ICl(acid), revealed Ca(2+)-independent inhibition by DIDS (IC(50) = 27 microM), glibenclamide (IC(50) = 31 microM) and DPC (IC(50) = 86 microM). ICl(acid) was neither affected by calix[4]arene nor by 9-AC. The order of potency for inhibition of ICl(acid) is DIDS approximately glibenclamide > DPC >> calix[4]arene, 9-AC. For comparison, the inhibitory profile of the swelling- and ATP-activated Cl(-) currents in Sertoli cells is DPC = DIDS >> glibenclamide = 9-AC for ICl(swell) and DPC = 9-AC = DIDS >> glibenclamide for ICl(ATP). This description provides new insights into the physiology and pharmacology of the endogenous Cl(-) channels expressed and potentially involved in fluid secretion in Sertoli cells.</p>","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"46 3","pages":"241-55"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2006013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26050940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Prenatal developmental changes in glucose transporters, intermediary metabolism and hormonal receptors related to the IGF/insulin-glucose axis in the heart and adipose tissue of bovines. 牛心脏和脂肪组织中与IGF/胰岛素-葡萄糖轴相关的葡萄糖转运蛋白、中间代谢和激素受体的产前发育变化
Reproduction, nutrition, development Pub Date : 2006-05-01 Epub Date: 2006-05-30 DOI: 10.1051/rnd:2006014
Jean-François Hocquette, Helga Sauerwein, Yumi Higashiyama, Brigitte Picard, Hiroyuki Abe
{"title":"Prenatal developmental changes in glucose transporters, intermediary metabolism and hormonal receptors related to the IGF/insulin-glucose axis in the heart and adipose tissue of bovines.","authors":"Jean-François Hocquette,&nbsp;Helga Sauerwein,&nbsp;Yumi Higashiyama,&nbsp;Brigitte Picard,&nbsp;Hiroyuki Abe","doi":"10.1051/rnd:2006014","DOIUrl":"https://doi.org/10.1051/rnd:2006014","url":null,"abstract":"<p><p>Glucose transporter ontogenesis is likely to play a key role in glucose uptake by foetal tissues in order to satisfy their energy requirements. We thus investigated developmental changes in the bovine heart and perirenal adipose tissue in two glucose transporter isoforms, namely GLUT1 and GLUT4, the latter being responsible for the regulation of glucose uptake by insulin. Other key players of the glucose/insulin axis were also assessed. Plasma glucose concentration in the foetus was lower at 8 and 8.5 months of age than previously. In the heart, GLUT1 protein level markedly decreased between 3 and 4 months of age, whereas the number of insulin and IGF-I binding sites continually decreased, especially between 7 and 8 or 8.5 months of age. On the contrary, the GLUT4 level increased until 8 months of age and remained high until 2 weeks after birth. The activities of enzymes of glucose metabolism (namely phosphofructokinase [PFK] and lactate dehydrogenase [LDH]) increased throughout gestation and reached a plateau at 6 and 8.5 months of age for PFK and LDH, respectively. The activities of enzymes involved in fatty acid metabolism increased especially at birth. In perirenal adipose tissue, high mitochondrial activity was detected before birth which is a characteristic of brown adipose tissue. Furthermore, lipoprotein lipase activity and GLUT4 protein level markedly increased to reach a maximum at 6-7 and 8 months of age, and sharply decreased thereafter, whereas GLUT1 protein level increased between 6 and 7 months of age. In conclusion, considerable changes in the regulation of the insulin/glucose axis were observed from 6 months onwards of foetal development in both the heart and adipose tissue of cattle, which probably alters the potential of these tissues to use glucose or fat as energy sources.</p>","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"46 3","pages":"257-72"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2006014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26051461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
Role of phosphodiesterase in cyclic AMP signaling in cultured rat granulosa cells. 磷酸二酯酶在培养大鼠颗粒细胞环AMP信号传导中的作用。
Reproduction, nutrition, development Pub Date : 2006-03-01 Epub Date: 2006-04-06 DOI: 10.1051/rnd:2006004
Zhengchao Wang, Lingmei Pan, Jinbiao Luo, Hao Wang, Fangxiong Shi
{"title":"Role of phosphodiesterase in cyclic AMP signaling in cultured rat granulosa cells.","authors":"Zhengchao Wang,&nbsp;Lingmei Pan,&nbsp;Jinbiao Luo,&nbsp;Hao Wang,&nbsp;Fangxiong Shi","doi":"10.1051/rnd:2006004","DOIUrl":"https://doi.org/10.1051/rnd:2006004","url":null,"abstract":"<p><p>Inactivation of the cyclic nucleotide signal in granulosa cells depends on a complex array of cyclic nucleotide phosphodiesterases (PDE). In order to examine the role of PDE in cyclic AMP (cAMP) signaling in granulosa cells, the present study examined the expression of PDE4D proteins and regulation of cAMP-PDE activities in cultured rat granulosa cells. The results of immunoblot analyses showed that two predominant PDE4D subtypes of approximately 80 and 70 kDa appeared when immature rat granulosa cells were treated with FSH. However, these two new subtypes presumed to be PDE4D proteins were not influenced by treatments of DETA/NO, cGMP and PKB inhibitor, LY294002. Immature rat granulosa cells treated with medium alone displayed low cAMP-PDE activity throughout 48 h of culture while those treated with FSH (2 ng.mL-1) showed a marked increase in cAMP-PDE activity between 6 and 12 h of culture, followed by a decline. The findings from the present study indicate that the increased cAMP-PDE activity by FSH is mainly related to the changes of PDE4D protein levels. However, the inhibitory effects of NO on cAMP accumulation in rat granulosa cells are not via the increased cAMP-PDE activity.</p>","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"46 2","pages":"179-87"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2006004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25958249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vivo oxidation of [9-14C] cyclic fatty acids derived from linolenic acid in the rat. 大鼠体内从亚麻酸中提取的[9-14C]环脂肪酸的氧化。
Reproduction, nutrition, development Pub Date : 2006-03-01 Epub Date: 2006-04-06 DOI: 10.1051/rnd:2006008
Lionel Bretillon, Olivier Loreau, Jean-Louis Sébédio, Frédéric Taran
{"title":"In vivo oxidation of [9-14C] cyclic fatty acids derived from linolenic acid in the rat.","authors":"Lionel Bretillon,&nbsp;Olivier Loreau,&nbsp;Jean-Louis Sébédio,&nbsp;Frédéric Taran","doi":"10.1051/rnd:2006008","DOIUrl":"https://doi.org/10.1051/rnd:2006008","url":null,"abstract":"<p><p>Heating oils and fats may lead to cyclization of polyunsaturated fatty acids, as for example linolenic acid. Cyclohexenyl and cyclopentenyl fatty acids are subsequently present in some edible oils and these are suspected to induce metabolic disorders. In a previous experiment using [1-14C] labeled molecules, we published that these cyclic fatty acids are beta oxidized to the same extent as linolenic acid, at least for the first cycle of beta oxidation. However, it is possible that the presence of a ring could alter the ability of the organism to fully oxidize the molecule. In order to test this hypothesis, we assessed the oxidative metabolism of cyclic fatty acids carrying a 14C atom at the vicinity of the ring. For this purpose, rats were force-fed from 1.1 to 1.3 MBq of a representative fraction of dietary cyclohexenyl cyclic fatty acid monomers of [9-14C] 9-(6-propyl-cyclohex-3-enyl)-non-8-enoic acids and 14CO2 production was monitored for 24h. The animals were then necropsied and the radioactivity was determined in different tissues. No consistent radioactivity was recovered as 14CO2 24h after administration of the molecules. Sixty percent of the radioactivity was recovered in the urine and 30% in the gastrointestinal tract. By combining our previous data on the oxidation of [1-14C] cyclic fatty acids and the present results, we suggest that cyclohexenyl fatty acids are first beta oxidized in a similar way as linolenic acid and that the remaining molecule carrying the ring is detoxified and eliminated in the urine and feces.</p>","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"46 2","pages":"189-93"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2006008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25958779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Genotoxical, teratological and biochemical effects of anthelmintic drug oxfendazole Maximum Residue Limit (MRL) in male and female mice. 驱虫药奥芬达唑最大残留限量(MRL)对雌雄小鼠的遗传毒性、致畸和生化影响。
Reproduction, nutrition, development Pub Date : 2006-03-01 Epub Date: 2006-04-06 DOI: 10.1051/rnd:2006007
Aida El-Makawy, Hasnaa A Radwan, Inas S Ghaly, A Abd El-Raouf
{"title":"Genotoxical, teratological and biochemical effects of anthelmintic drug oxfendazole Maximum Residue Limit (MRL) in male and female mice.","authors":"Aida El-Makawy,&nbsp;Hasnaa A Radwan,&nbsp;Inas S Ghaly,&nbsp;A Abd El-Raouf","doi":"10.1051/rnd:2006007","DOIUrl":"https://doi.org/10.1051/rnd:2006007","url":null,"abstract":"<p><p>Oxfendazole, methyl-5 (6)-phenylsulfinyl-2-benzimidazole carbamate, is a member of the benzimidazole family of anthelmintics. Anthelmintic benzimidazoles are widely used in meat producing animals (cattle, sheep and pigs) for control of endoparasites. The extensive use of veterinary drugs in food-producing animals can cause the presence of small quantities of the drug residues in food. Maximum residue limit or \"MRL\" means the maximum concentration of residue resulting from the use of a veterinary medicinal product which may be legally permitted recognized as acceptable in food. The FAO/WHO Expert Committee on Food Additives (1999) evaluations of toxicological and residue data, reported that oxfendazole (MRL) has toxicological hazards on human health. The toxicity of oxfendazole (MRL) was tested in male and female mice and their fetuses. Chromosomal aberrations, teratological examination and biochemical analysis were the parameters used in this study. The results show that oxfendazole MRL induced a mutagenic effect in all tested cell types. Also, oxfendazole exhibit embryotoxicity including teratogenicity. The biochemical results show that oxfendazole induced a disturbance in the different biochemical contents of all tested tissues. So, we must increase the attention paid to the potential risk of oxfendazole residues in human beings and should stress the need for careful control to ensure adherence to the prescribed withdrawal time of this drug.</p>","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"46 2","pages":"139-56"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2006007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25958245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 27
Long term effect of post-weaning rhythm on the body fat and performance of rabbit doe. 断奶后节律对兔母鹿体脂和生产性能的长期影响。
Reproduction, nutrition, development Pub Date : 2006-03-01 Epub Date: 2006-04-06 DOI: 10.1051/rnd:2006009
Cesare Castellini, Alessandro Dal Bosco, Raffaella Cardinali
{"title":"Long term effect of post-weaning rhythm on the body fat and performance of rabbit doe.","authors":"Cesare Castellini,&nbsp;Alessandro Dal Bosco,&nbsp;Raffaella Cardinali","doi":"10.1051/rnd:2006009","DOIUrl":"https://doi.org/10.1051/rnd:2006009","url":null,"abstract":"<p><p>Reproductive protocols based on standard (Control: 11 days post-partum) or extended rhythm (PW: post-weaning at 27 days post-partum) were compared. Two groups of fifty 19-week-old New Zealand White females were inseminated for seven consecutive cycles. The kits were weaned at 26 days. On the day of AI, all the does were submitted to ultrasound scanning of the perirenal regions to measure fat thickness. The fertility rate and several indexes of efficiency were calculated. Fat thickness, estimated perirenal fat and live weight were higher in PW does. The does submitted to post-weaning rhythm had a higher sexual receptivity (P < 0.01), and fertility rate (P < 0.01) whereas litter size and pre-weaning mortality were not affected. Primiparous Control does showed a particularly low fertility rate; the value increased successively but was always lower than in PW does. PW rhythm in comparison with the standard one seemed more adapted to doe reproductive physiology even if there was a lower production (35.0 vs. 38.8 rabbit sold/year) and risk of fatness (18% of multiparous does).</p>","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"46 2","pages":"195-204"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2006009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25958251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 47
Demecolcine-induced enucleation of sheep meiotically maturing oocytes. deecolcine诱导绵羊减数分裂成熟卵母细胞去核。
Reproduction, nutrition, development Pub Date : 2006-03-01 Epub Date: 2006-04-06 DOI: 10.1051/rnd:2006002
Jian Hou, Tinghua Lei, Lei Liu, Xiuhong Cui, Xiaorong An, Yongfu Chen
{"title":"Demecolcine-induced enucleation of sheep meiotically maturing oocytes.","authors":"Jian Hou,&nbsp;Tinghua Lei,&nbsp;Lei Liu,&nbsp;Xiuhong Cui,&nbsp;Xiaorong An,&nbsp;Yongfu Chen","doi":"10.1051/rnd:2006002","DOIUrl":"https://doi.org/10.1051/rnd:2006002","url":null,"abstract":"<p><p>The objective of this study was to investigate the possible effect of demecolcine, a microtubule-disrupting reagent, on induced enucleation (IE) of sheep meiotically maturing oocytes. Immunofluorescent staining with anti-tubulin antibodies was used to examine the spindle status of the oocytes. When the oocytes with intact germinal vesicles (GV) were cultured in the medium containing various concentrations of demecolcine (0.01 to 0.4 microg.mL-1) for 20 to 22 h, the spindle microtubule organization and first polar body (PB1) extrusion were inhibited by demecolcine in a dose-dependent manner. The highest IE rate (58.1%) was from the treatment with 0.04 microg.mL-1 demecolcine. Demecolcine treatment applied after germinal vesicle breakdown (GVBD) or at metaphase (M) yielded a PB1 extrusion rate and IE efficiency similar to the treatment applied at the onset of maturation. Analysis by immunofluorescence showed that both nonspindle microtubules and spindle microtubules were significantly disorganized by demecolcine. Combination treatment with demecolcine and cycloheximide (CHX) or 6-dimethylaminopurine (6-DMAP) led to single pronuclear formation rather than PB1 extrusion. When demecolcine-treated oocytes were transferred into demecolcine-free medium, the ability to extrude PB1 was quickly restored and a 72.1% IE rate was obtained following such treatment. These results demonstrate that demecolcine can be used as a potential reagent for induced enucleation of sheep meiotically maturing oocytes and may greatly facilitate research in nuclear transfer.</p>","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"46 2","pages":"219-26"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2006002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25958777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Effects of a bovine colostrum-supplemented diet on some gut parameters in weaned piglets. 牛初乳添加日粮对断奶仔猪肠道某些参数的影响。
Reproduction, nutrition, development Pub Date : 2006-03-01 Epub Date: 2006-04-06 DOI: 10.1051/rnd:2006006
Antoine Huguet, Bernard Sève, Jean Le Dividich, Isabelle Le Huërou-Luron
{"title":"Effects of a bovine colostrum-supplemented diet on some gut parameters in weaned piglets.","authors":"Antoine Huguet,&nbsp;Bernard Sève,&nbsp;Jean Le Dividich,&nbsp;Isabelle Le Huërou-Luron","doi":"10.1051/rnd:2006006","DOIUrl":"https://doi.org/10.1051/rnd:2006006","url":null,"abstract":"<p><p>The present study investigated the effects of a bovine colostrum-supplemented diet on gut post-weaning adaptation and health in piglets. Thirty-six 21-d-old piglets were allocated to one of the three following dietary treatments: sow-reared (SR), weaned on a control starter diet (WCtrl) or on a starter diet supplemented with bovine colostrum (WCol) until slaughter at 28 d or 35 d of age. Gastric pH and intestinal bacteriological, structural and functional parameters were determined. Compared to WCtrl, the gastric pH was lower (P < 0.05) and the duodenal lactobacilli:coliform ratio was higher (P = 0.05) in WCol piglets. The relative small intestine weight was 18% (P < 0.05) higher in WCol piglets than in SR piglets. Duodenal villous height was lower (P < 0.01) in WCtrl than in SR piglets, whereas the value for WCol piglets was intermediate. The weaning-increased crypt cell proliferation was not affected by bovine colostrum supplementation. The mucosal ribosomal capacity was higher (P < 0.05) in W than in SR piglets. In conclusion, a diet supplemented with colostrum induced, although not always significantly, variations of gut parameters, suggesting that globally, colostrum may limit weaning-induced gut structural and microbial alterations. The observed effects occurred early and were maintained throughout the post-weaning adaptive phase.</p>","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"46 2","pages":"167-78"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2006006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25958248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 35
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