Retrovirology最新文献_第10页

Abstract withdrawn 抽象撤销

IF 3.3 3区医学

Retrovirology Pub Date : 2018-10-01 DOI: 10.1186/1742-4690-2-S1-P136

引用次数: 0

A CRISPR screen for factors regulating SAMHD1 degradation identifies IFITMs as potent inhibitors of lentiviral particle delivery. 通过CRISPR筛选SAMHD1降解调节因子，发现IFITMs是慢病毒颗粒递送的有效抑制剂。

IF 3.3 3区医学

Retrovirology Pub Date : 2018-03-20 DOI: 10.1186/s12977-018-0409-2

Ferdinand Roesch, Molly OhAinle, Michael Emerman

{"title":"A CRISPR screen for factors regulating SAMHD1 degradation identifies IFITMs as potent inhibitors of lentiviral particle delivery.","authors":"Ferdinand Roesch, Molly OhAinle, Michael Emerman","doi":"10.1186/s12977-018-0409-2","DOIUrl":"https://doi.org/10.1186/s12977-018-0409-2","url":null,"abstract":"The InterFeron Induced TransMembrane (IFITM) proteins are interferon stimulated genes that restrict many viruses, including HIV-1. SAMHD1 is another restriction factor blocking replication of HIV-1 and other viruses. Some lentiviruses evolved Vpx/Vpr proteins to degrade SAMHD1. However, this viral antagonism can be perturbed by host mechanisms: a recent study showed that in interferon (IFN) treated THP1 cells, Vpx is unable to degrade SAMHD1. In the present work, we designed an Interferon Stimulated Genes (ISGs)-targeted CRISPR knockout screen in order to identify ISGs regulating this phenotype. We found that IFITM proteins contribute to the IFNα-mediated protection of SAMHD1 by blocking VSV-G-mediated entry of the lentiviral particles delivering Vpx. Consistent with this, IFNα treatment and IFITM expression had no effect when the A-MLV envelope was used for pseudotyping. Using an assay measuring viral entry, we show that IFNα and IFITMs directly block the delivery of Vpx into cells by inhibiting VSV-G viral fusion. Strikingly, the VSV-G envelope was significantly more sensitive to this IFNα entry block and to IFITMs than HIV-1's natural envelope. This highlights important differences between VSV-G pseudotyped and wild-type HIV-1, in particular relative to the pathways they use for viral entry, suggesting that HIV-1 may have evolved to escape restriction factors blocking entry.","PeriodicalId":21123,"journal":{"name":"Retrovirology","volume":"15 1","pages":"26"},"PeriodicalIF":3.3,"publicationDate":"2018-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12977-018-0409-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9989470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Determinants of adherence to physical activity guidelines among overweight and obese African American breast cancer survivors: implications for an intervention approach. 超重和肥胖的非裔美国乳腺癌幸存者遵守体育锻炼指南的决定因素：对干预方法的影响。

IF 3.1 3区医学

Retrovirology Pub Date : 2018-02-01 Epub Date: 2016-11-14 DOI: 10.1080/13557858.2016.1256376

Selina A Smith, Benjamin E Ansa, Wonsuk Yoo, Mary S Whitehead, Steven S Coughlin

{"title":"Determinants of adherence to physical activity guidelines among overweight and obese African American breast cancer survivors: implications for an intervention approach.","authors":"Selina A Smith, Benjamin E Ansa, Wonsuk Yoo, Mary S Whitehead, Steven S Coughlin","doi":"10.1080/13557858.2016.1256376","DOIUrl":"10.1080/13557858.2016.1256376","url":null,"abstract":"Objective: Public health agencies encourage breast cancer survivors (BCSs) to follow their physical activity guidelines (PAGs). However, adherence to these guidelines is low. African American (AA) BCSs are more often overweight or obese and less likely than women of other races to report adherence to physical activity recommendations. This study examined socioeconomic, clinical, and psychosocial correlates with meeting PAGs.Design: AA women diagnosed and treated for breast cancer and participating in a breast cancer support group (N = 193) completed a lifestyle assessment tool capturing demographic characteristics; breast cancer diagnosis and treatment history; health-related quality of life; weight history, including body mass index and post-diagnosis weight gain; and physical activity. Logistic regressions were used to determine if these covariates were associated with meeting [>8.3 metabolic equivalent task (MET) hr/wk]; partially meeting (4.15-8.3 MET hr/wk); or not meeting (<4.15 MET hr/wk) PAGs.Results: Only 54% of AA BCSs reported meeting current PAGs. Participants reporting weight gain of ≤5 lbs post-diagnosis, and those who received surgical treatment for breast cancer were more likely to complete at least 8.3 MET hr/wk. Better physical functioning and lesser pain intensity were associated with meeting PAGs.Conclusion: Several factors influence physical activity behaviors and are likely to be important in developing effective interventions to assist AA survivors manage their weight. It is essential that providers and breast cancer support groups that assist survivors to remain physically active and to manage their weight should be aware of these factors. These findings may help generate hypotheses for future research to undergird efforts to increase physical activity among African American BCSs.","PeriodicalId":21123,"journal":{"name":"Retrovirology","volume":"9 1","pages":"194-206"},"PeriodicalIF":3.1,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88388707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a homogenous structural basis for oligomerization by retroviral Rev-like proteins. 逆转录病毒rev样蛋白寡聚化的同质结构基础的鉴定。

IF 3.3 3区医学

Retrovirology Pub Date : 2017-08-22 DOI: 10.1186/s12977-017-0366-1

Chijioke N Umunnakwe, Karin S Dorman, Drena Dobbs, Susan Carpenter

{"title":"Identification of a homogenous structural basis for oligomerization by retroviral Rev-like proteins.","authors":"Chijioke N Umunnakwe, Karin S Dorman, Drena Dobbs, Susan Carpenter","doi":"10.1186/s12977-017-0366-1","DOIUrl":"https://doi.org/10.1186/s12977-017-0366-1","url":null,"abstract":"Background: Rev-like proteins are post-transcriptional regulatory proteins found in several retrovirus genera, including lentiviruses, betaretroviruses, and deltaretroviruses. These essential proteins mediate the nuclear export of incompletely spliced viral RNA, and act by tethering viral pre-mRNA to the host CRM1 nuclear export machinery. Although all Rev-like proteins are functionally homologous, they share less than 30% sequence identity. In the present study, we computationally assessed the extent of structural homology among retroviral Rev-like proteins within a phylogenetic framework.Results: We undertook a comprehensive analysis of overall protein domain architecture and predicted secondary structural features for representative members of the Rev-like family of proteins. Similar patterns of α-helical domains were identified for Rev-like proteins within each genus, with the exception of deltaretroviruses, which were devoid of α-helices. Coiled-coil oligomerization motifs were also identified for most Rev-like proteins, with the notable exceptions of HIV-1, the deltaretroviruses, and some small ruminant lentiviruses. In Rev proteins of primate lentiviruses, the presence of predicted coiled-coil motifs segregated within specific primate lineages: HIV-1 descended from SIVs that lacked predicted coiled-coils in Rev whereas HIV-2 descended from SIVs that contained predicted coiled-coils in Rev. Phylogenetic ancestral reconstruction of coiled-coils for all Rev-like proteins predicted a single origin for the coiled-coil motif, followed by three losses of the predicted signal. The absence of a coiled-coil signal in HIV-1 was associated with replacement of canonical polar residues with non-canonical hydrophobic residues. However, hydrophobic residues were retained in the key 'a' and 'd' positions, and the α-helical region of HIV-1 Rev oligomerization domain could be modeled as a helical wheel with two predicted interaction interfaces. Moreover, the predicted interfaces mapped to the dimerization and oligomerization interfaces in HIV-1 Rev crystal structures. Helical wheel projections of other retroviral Rev-like proteins, including endogenous sequences, revealed similar interaction interfaces that could mediate oligomerization.Conclusions: Sequence-based computational analyses of Rev-like proteins, together with helical wheel projections of oligomerization domains, reveal a conserved homogeneous structural basis for oligomerization by retroviral Rev-like proteins.","PeriodicalId":21123,"journal":{"name":"Retrovirology","volume":"14 1","pages":"40"},"PeriodicalIF":3.3,"publicationDate":"2017-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12977-017-0366-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9941758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Erratum to: Are T cells the only HIV-1 reservoir? 勘误表：T细胞是唯一的HIV-1宿主吗？

IF 3.3 3区医学

Retrovirology Pub Date : 2017-02-08 DOI: 10.1186/s12977-017-0333-x

A. Kandathil, S. Sugawara, A. Balagopal

引用次数: 2

Toll-like receptor 3 activation selectively reverses HIV latency in microglial cells toll样受体3激活选择性地逆转小胶质细胞中的HIV潜伏期

IF 3.3 3区医学

Retrovirology Pub Date : 2017-02-06 DOI: 10.1186/s12977-017-0335-8

David Alvarez-Carbonell, Yoelvis Garcia-Mesa, S. Milne, B. Das, C. Dobrowolski, R. Rojas, J. Karn

引用次数: 81

A purine-rich element in foamy virus pol regulates env splicing and gag/pol expression 泡沫状病毒pol中富含嘌呤的元素调控env剪接和gag/pol表达