Restorative Dentistry & Endodontics最新文献

{"title":"Buckling resistance, torque, and force generation during retreatment with D-RaCe, HyFlex Remover, and Mtwo retreatment files.","authors":"Yoojin Kim,&nbsp;Seok Woo Chang,&nbsp;Soram Oh","doi":"10.5395/rde.2023.48.e10","DOIUrl":"https://doi.org/10.5395/rde.2023.48.e10","url":null,"abstract":"<p><strong>Objectives: </strong>This study compared the buckling resistance of 3 nickel-titanium (NiTi) retreatment file systems and the torque/force generated during retreatment.</p><p><strong>Materials and methods: </strong>The buckling resistance was compared among the D-RaCe (DR2), HyFlex Remover, and Mtwo R25/05 retreatment systems. J-shaped canals within resin blocks were prepared with ProTaper NEXT X3 and obturated by the single-cone technique with AH Plus. After 4 weeks, 4 mm of gutta-percha in the coronal aspect was removed with Gates-Glidden drills. Retreatment was then performed using DR1 (size 30, 10% taper) followed by DR2 (size 25, 4% taper), HyFlex Remover (size 30, 7% taper), or Mtrwo R25/05 (size 25, 5% taper) (15 specimens in each group). Further apical preparation was performed with WaveOne Gold Primary. The clockwise torque and upward force generated during retreatment were recorded. After retreatment, resin blocks were examined using stereomicroscopy, and the percentage of residual filling material in the canal area was calculated. Data were analyzed using 1-way analysis of variance with the Tukey test.</p><p><strong>Results: </strong>The HyFlex Remover files exhibited the greatest buckling resistance (<i>p</i> < 0.05), followed by the Mtwo R25/05. The HyFlex Remover and Mtwo R25/05 files generated the highest maximum clockwise torque and upward force, respectively (<i>p</i> < 0.05). The DR1 and DR2 files generated the least upward force and torque (<i>p</i> < 0.05). The percentage of residual filling material after retreatment was not significantly different between file systems (<i>p</i> > 0.05).</p><p><strong>Conclusions: </strong>NiTi retreatment instruments with higher buckling resistance generated greater clockwise torque and upward force.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"48 1","pages":"e10"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d8/44/rde-48-e10.PMC9982244.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10849946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of endodontic sealer on postoperative pain: a network meta-analysis.","authors":"Cynthia Maria Chaves Monteiro,&nbsp;Ana Cristina Rodrigues Martins,&nbsp;Alessandra Reis,&nbsp;Juliana Larocca de Geus","doi":"10.5395/rde.2023.48.e5","DOIUrl":"https://doi.org/10.5395/rde.2023.48.e5","url":null,"abstract":"<p><p>This systematic review and network meta-analysis aimed to answer the following focused research question: \"Does the type of endodontic sealer affect the postoperative pain in patients who received endodontic treatment?\" Different databases and grey literature were surveyed. Only one randomized controlled trial were included. The risk of bias in the studies was evaluated by using the Cochrane Collaboration's tool. A random-effects meta-analysis was conducted to compare the risk and intensity of postoperative pain. The quality of the body of evidence was assessed using the Grading of Recommendations Assessment, Development, and Evaluation approach. Out of 11,601 studies, 15 remained for qualitative analyses and 12 for meta-analysis. Seven studies were classified at high risk of bias, and 8 studies raised some concerns. No significant differences between the endodontic materials were observed in the direct comparisons, both in risk and in intensity of postoperative pain (pairwise comparisons with 2 studies: I² = 0%; <i>p</i> > 0.05 and 8 studies: I² = 23%; <i>p</i> > 0.05, respectively). The certainty of the evidence was graded as low or moderate. There was no difference in the risk and intensity of postoperative pain after filling with different endodontic sealers. Further systematic reviews should be conducted.</p><p><strong>Trial registration: </strong>PROSPERO Identifier: CRD42020215314.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"48 1","pages":"e5"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a7/83/rde-48-e5.PMC9982236.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10849943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical properties of a calcium aluminate cement containing nanoparticles of zinc oxide.","authors":"Amanda Freitas da Rosa,&nbsp;Thuany Schmitz Amaral,&nbsp;Maria Eduarda Paz Dotto,&nbsp;Taynara Santos Goulart,&nbsp;Hebert Luís Rossetto,&nbsp;Eduardo Antunes Bortoluzzi,&nbsp;Cleonice da Silveira Teixeira,&nbsp;Lucas da Fonseca Roberti Garcia","doi":"10.5395/rde.2023.48.e3","DOIUrl":"https://doi.org/10.5395/rde.2023.48.e3","url":null,"abstract":"<p><strong>Objectives: </strong>This study evaluated the effect of different nanoparticulated zinc oxide (nano-ZnO) and conventional-ZnO ratios on the physicochemical properties of calcium aluminate cement (CAC).</p><p><strong>Materials and methods: </strong>The conventional-ZnO and nano-ZnO were added to the cement powder in the following proportions: G1 (20% conventional-ZnO), G2 (15% conventional-ZnO + 5% nano-ZnO), G3 (12% conventional-ZnO + 3% nano-ZnO) and G4 (10% conventional-ZnO + 5% nano-ZnO). The radiopacity (R_ad), setting time (S_et), dimensional change (D_c), solubility (S_ol), compressive strength (C_st), and pH were evaluated. The nano-ZnO and CAC containing conventional-ZnO were also assessed using scanning electron microscopy, transmission electron microscopy, and energy-dispersive X-ray spectroscopy. Radiopacity data were analyzed by the 1-way analysis of variance (ANOVA) and Bonferroni tests (<i>p</i> < 0.05). The data of the other properties were analyzed by the ANOVA, Tukey, and Fisher tests (<i>p</i> < 0.05).</p><p><strong>Results: </strong>The nano-ZnO and CAC containing conventional-ZnO powders presented particles with few impurities and nanometric and micrometric sizes, respectively. G1 had the highest R_ad mean value (<i>p</i> < 0.05). When compared to G1, groups containing nano-ZnO had a significant reduction in the S_et (<i>p</i> < 0.05) and lower values of D_c at 24 hours (<i>p</i> < 0.05). The C_st was higher for G4, with a significant difference for the other groups (<i>p</i> < 0.05). The S_ol did not present significant differences among groups (<i>p</i> > 0.05).</p><p><strong>Conclusions: </strong>The addition of nano-ZnO to CAC improved its dimensional change, setting time, and compressive strength, which may be promising for the clinical performance of this cement.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"48 1","pages":"e3"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a2/82/rde-48-e3.PMC9982238.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10849942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Push-out bond strength and intratubular biomineralization of a hydraulic root-end filling material premixed with dimethyl sulfoxide as a vehicle.","authors":"Ju-Ha Park,&nbsp;Hee-Jin Kim,&nbsp;Kwang-Won Lee,&nbsp;Mi-Kyung Yu,&nbsp;Kyung-San Min","doi":"10.5395/rde.2023.48.e8","DOIUrl":"https://doi.org/10.5395/rde.2023.48.e8","url":null,"abstract":"<p><strong>Objectives: </strong>This study was designed to evaluate the parameters of bonding performance to root dentin, including push-out bond strength and dentinal tubular biomineralization, of a hydraulic bioceramic root-end filling material premixed with dimethyl sulfoxide (Endocem MTA Premixed) in comparison to a conventional powder-liquid-type cement (ProRoot MTA).</p><p><strong>Materials and methods: </strong>The root canal of a single-rooted premolar was filled with either ProRoot MTA or Endocem MTA Premixed (<i>n</i> = 15). A slice of dentin was obtained from each root. Using the sliced specimen, the push-out bond strength was measured, and the failure pattern was observed under a stereomicroscope. The apical segment was divided into halves; the split surface was observed under a scanning electron microscope, and intratubular biomineralization was examined by observing the precipitates formed in the dentinal tubule. Then, the chemical characteristics of the precipitates were evaluated with energy-dispersive X-ray spectroscopic (EDS) analysis. The data were analyzed using the Student's <i>t</i>-test followed by the Mann-Whitney <i>U</i> test (<i>p</i> < 0.05).</p><p><strong>Results: </strong>No significant difference was found between the 2 tested groups in push-out bond strength, and cohesive failure was the predominant failure type. In both groups, flake-shaped precipitates were observed along dentinal tubules. The EDS analysis indicated that the mass percentage of calcium and phosphorus in the precipitate was similar to that found in hydroxyapatite.</p><p><strong>Conclusions: </strong>Regarding bonding to root dentin, Endocem MTA Premixed may have potential for use as an acceptable root-end filling material.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"48 1","pages":"e8"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/dc/6d/rde-48-e8.PMC9982241.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9412776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of torsional and cyclic fatigue resistance of ProGlider, WaveOne Gold Glider, and TruNatomy Glider in simulated curved canal.","authors":"Pedro de Souza Dias,&nbsp;Augusto Shoji Kato,&nbsp;Carlos Eduardo da Silveira Bueno,&nbsp;Rodrigo Ricci Vivan,&nbsp;Marco Antonio Hungaro Duarte,&nbsp;Pedro Henrique Souza Calefi,&nbsp;Rina Andréa Pelegrine","doi":"10.5395/rde.2023.48.e4","DOIUrl":"https://doi.org/10.5395/rde.2023.48.e4","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to compare the torsional and cyclic fatigue resistance of ProGlider (PG), WaveOne Gold Glider (WGG), and TruNatomy Glider (TNG).</p><p><strong>Materials and methods: </strong>A total of 15 instruments of each glide path system (<i>n</i> = 15) were used for each test. A custom-made device simulating an angle of 90° and a radius of 5 millimeters was used to assess cyclic fatigue resistance, with calculation of number of cycles to failure. Torsional fatigue resistance was assessed by maximum torque and angle of rotation. Fractured instruments were examined by scanning electron microscopy (SEM). Data were analyzed with Shapiro-Wilk and Kruskal-Wallis tests, and the significance level was set at 5%.</p><p><strong>Results: </strong>The WGG group showed greater cyclic fatigue resistance than the PG and TNG groups (<i>p</i> < 0.05). In the torsional fatigue test, the TNG group showed a higher angle of rotation, followed by the PG and WGG groups (<i>p</i> < 0.05). The TNG group was superior to the PG group in torsional resistance (<i>p</i> < 0.05). SEM analysis revealed ductile morphology, typical of the 2 fracture modes: cyclic fatigue and torsional fatigue.</p><p><strong>Conclusions: </strong>Reciprocating WGG instruments showed greater cyclic fatigue resistance, while TNG instruments were better in torsional fatigue resistance. The significance of these findings lies in the identification of the instruments' clinical applicability to guide the choice of the most appropriate instrument and enable the clinician to provide a more predictable glide path preparation.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"48 1","pages":"e4"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c5/cc/rde-48-e4.PMC9982240.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10849939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of gingival blood flow on pulpal blood flow detection using ultrasound Doppler flowmetry: animal study.","authors":"Dohyun Kim,&nbsp;Hyoung-Seok Ko,&nbsp;Soo-Yeon Park,&nbsp;Seung-Yeon Ryu,&nbsp;Sung-Ho Park","doi":"10.5395/rde.2023.48.e9","DOIUrl":"https://doi.org/10.5395/rde.2023.48.e9","url":null,"abstract":"<p><strong>Objectives: </strong>This study evaluated the effect of adjacent gingival blood flow on detection of pulpal blood flow (PBF) using ultrasound Doppler flowmetry (UDF) through animal study.</p><p><strong>Materials and methods: </strong>The study included 36 right and left maxillary the third incisors and canines in 9 experimental dogs. The study included 2 main steps: In the first step, the pulse sound level (PSL) was recorded on the cervical part of each tooth without flap elevation (Group 1), with flap elevation (Group 2), and after it was repositioned in place (Group 3). In the second step, the PSL was recorded on the cervical part of each tooth (Group 4), after pulpotomy (Group 5), after partial pulp extirpation (Group 6), after complete extirpation (Group 7), and after canal filling (Group 8). In Groups 5-8, the study was performed with and without flap elevation in the left and right teeth, respectively. The PSL was graded as follows: 0, inaudible; 1, heard faintly; and 2, heard well. The difference between each group was analyzed using Friedman's test with Wilcoxon signed-rank tests (α = 0.05).</p><p><strong>Results: </strong>In step 1, the PSL results were Group 1 > 2 and 3. In step 2, there was no significant difference between the groups when the flap was not elevated, while PSL results were Group 4 > 5 ≥ 6 and 7 ≥ 8 when the flap was elevated.</p><p><strong>Conclusions: </strong>PBF is affected by gingival blood flow when measured with UDF. UDF measurements require isolation of gingiva from the tooth.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"48 1","pages":"e9"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/51/c2/rde-48-e9.PMC9982237.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10849940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial and cytotoxic properties of calcium-enriched mixture cement, Iranian propolis, and propolis with herbal extracts in primary dental pulp stem cells.","authors":"Mohammad Esmaeilzadeh,&nbsp;Shirin Moradkhani,&nbsp;Fahimeh Daneshyar,&nbsp;Mohammad Reza Arabestani,&nbsp;Sara Soleimani Asl,&nbsp;Soudeh Tayebi,&nbsp;Maryam Farhadian","doi":"10.5395/rde.2023.48.e2","DOIUrl":"https://doi.org/10.5395/rde.2023.48.e2","url":null,"abstract":"<p><strong>Objectives: </strong>In this study, natural substances were introduced as primary dental pulp caps for use in pulp therapy, and the antimicrobial and cytotoxic properties of these substances were investigated.</p><p><strong>Materials and methods: </strong>In this <i>in vitro</i> study, the antimicrobial properties of calcium-enriched mixture (CEM) cement, propolis, and propolis individually combined with the extracts of several medicinal plants were investigated against <i>Enterococcus faecalis</i>, <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, and <i>Staphylococcus aureus</i>. Then, the cytotoxicity of each substance or mixture against pulp stem cells extracted from 30 primary healthy teeth was evaluated at 4 concentrations. Data were gathered via observation, and optical density values were obtained using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) test and recorded. SPSS software version 23 was used to analyze the data. Data were evaluated using 2-way analysis of variance and the Tukey test.</p><p><strong>Results: </strong>Regarding antimicrobial properties, thyme alone and thyme + propolis had the lowest minimum inhibitory concentrations (MICs) against the growth of <i>S. aureus</i>, <i>E. coli</i>, and <i>P. aeruginosa</i> bacteria. For <i>E. faecalis</i>, thyme + propolis had the lowest MIC, followed by thyme alone. At 24 and 72 hours, thyme + propolis, CEM cement, and propolis had the greatest bioviability in the primary dental pulp stem cells, and lavender + propolis had the lowest bioviability.</p><p><strong>Conclusions: </strong>Of the studied materials, thyme + propolis showed the best results in the measures of practical performance as a dental pulp cap.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"48 1","pages":"e2"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/97/a1/rde-48-e2.PMC9982242.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9412780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of surrounding and underlying shades on the color adjustment potential of a single-shade composite used in a thin layer.","authors":"Mariana Silva Barros,&nbsp;Paula Fernanda Damasceno Silva,&nbsp;Márcia Luciana Carregosa Santana,&nbsp;Rafaella Mariana Fontes Bragança,&nbsp;André Luis Faria-E-Silva","doi":"10.5395/rde.2023.48.e7","DOIUrl":"https://doi.org/10.5395/rde.2023.48.e7","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to evaluate the surrounding and underlying shades' effect on the color adjustment potential (CAP) of a single-shade composite used in a thin layer.</p><p><strong>Materials and methods: </strong>Cylinder specimens (1.0 mm thick) were built with the Vittra APS Unique composite, surrounded (dual specimens) or not (simple specimens) by a control composite (shade A1, A2, or A3). Simple specimens were also built only with the control composites. Each specimen's color was measured against white and black backgrounds or the simple control specimens with a spectrophotometer (CIELAB system). The whiteness index for dentistry (WI_D) and translucency parameters (TP₀₀) were calculated for simple specimens. Differences (ΔE₀₀) in color between the simple/dual specimens and the controls were calculated. The CAP was calculated based on the ratios between data from simple and dual specimens.</p><p><strong>Results: </strong>The Vittra APS Unique composite showed higher WI_D and TP₀₀ values than the controls. The highest values of ΔE₀₀ were observed among simple specimens. The color measurements of Vittra APS Unique (simple or dual) against the control specimens presented the lowest color differences. Only surrounding the single-shade composite with a shaded composite barely impacted the ΔE₀₀. The highest CAP values were obtained using a shaded composite under simple or dual specimens.</p><p><strong>Conclusions: </strong>The CAP of Vittra APS Unique was strongly affected by the underlying shade, while surrounding this composite with a shaded one barely affected its color adjustment.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"48 1","pages":"e7"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bd/5d/rde-48-e7.PMC9982245.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9412777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Appreciation to reviewers and announcement of a new editorial board","authors":"Kyung-San Min","doi":"10.5395/rde.2023.48.e11","DOIUrl":"https://doi.org/10.5395/rde.2023.48.e11","url":null,"abstract":"","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49519096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-plasticity mineral trioxide aggregate and its effects on M1 and M2 macrophage viability and adherence, phagocyte activity, production of reactive oxygen species, and cytokines.","authors":"Betânia Canal Vasconcellos, Layara Cristine Tomaz Tavares, Danilo Couto da Silva, Francielen Oliveira Fonseca, Francine Benetti, Antônio Paulino Ribeiro Sobrinho, Warley Luciano Fonseca Tavares","doi":"10.5395/rde.2023.48.e6","DOIUrl":"10.5395/rde.2023.48.e6","url":null,"abstract":"<p><strong>Objectives: </strong>This study evaluated the effects of high-plasticity mineral trioxide aggregate (MTA-HP) on the activity of M1 and M2 macrophages, compared to white MTA (Angelus).</p><p><strong>Materials and methods: </strong>Peritoneal inflammatory M1 (from C57BL/6 mice) and M2 (from BALB/c mice) macrophages were cultured in the presence of the tested materials. Cell viability (MTT and trypan blue assays), adhesion, phagocytosis, reactive oxygen species (ROS) production, and tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-β production were evaluated. Parametric analysis of variance and the non-parametric Kruskal-Wallis test were used. Results were considered significant when <i>p</i> < 0.05.</p><p><strong>Results: </strong>The MTT assay revealed a significant decrease in M1 metabolism with MTA-HP at 24 hours, and with MTA and MTA-HP later. The trypan blue assay showed significantly fewer live M1 at 48 hours and live M2 at 48 and 72 hours with MTA-HP, compared to MTA. M1 and M2 adherence and phagocytosis showed no significant differences compared to control for both materials. Zymosan A stimulated ROS production by macrophages. In the absence of interferon-γ, TNF-α production by M1 did not significantly differ between groups. For M2, both materials showed higher TNF-α production in the presence of the stimulus, but without significant between-group differences. Likewise, TGF-β production by M1 and M2 macrophages was not significantly different between the groups.</p><p><strong>Conclusions: </strong>M1 and M2 macrophages presented different viability in response to MTA and MTA-HP at different time points. Introducing a plasticizer into the MTA vehicle did not interfere with the activity of M1 and M2 macrophages.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"48 1","pages":"e6"},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/04/78/rde-48-e6.PMC9982239.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9412779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}