Restorative Dentistry & Endodontics最新文献

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Antimicrobial and cytotoxic properties of calcium-enriched mixture cement, Iranian propolis, and propolis with herbal extracts in primary dental pulp stem cells. 富钙水泥、伊朗蜂胶和含草药提取物蜂胶在原代牙髓干细胞中的抗菌和细胞毒性。
Restorative Dentistry & Endodontics Pub Date : 2023-02-01 DOI: 10.5395/rde.2023.48.e2
Mohammad Esmaeilzadeh, Shirin Moradkhani, Fahimeh Daneshyar, Mohammad Reza Arabestani, Sara Soleimani Asl, Soudeh Tayebi, Maryam Farhadian
{"title":"Antimicrobial and cytotoxic properties of calcium-enriched mixture cement, Iranian propolis, and propolis with herbal extracts in primary dental pulp stem cells.","authors":"Mohammad Esmaeilzadeh,&nbsp;Shirin Moradkhani,&nbsp;Fahimeh Daneshyar,&nbsp;Mohammad Reza Arabestani,&nbsp;Sara Soleimani Asl,&nbsp;Soudeh Tayebi,&nbsp;Maryam Farhadian","doi":"10.5395/rde.2023.48.e2","DOIUrl":"https://doi.org/10.5395/rde.2023.48.e2","url":null,"abstract":"<p><strong>Objectives: </strong>In this study, natural substances were introduced as primary dental pulp caps for use in pulp therapy, and the antimicrobial and cytotoxic properties of these substances were investigated.</p><p><strong>Materials and methods: </strong>In this <i>in vitro</i> study, the antimicrobial properties of calcium-enriched mixture (CEM) cement, propolis, and propolis individually combined with the extracts of several medicinal plants were investigated against <i>Enterococcus faecalis</i>, <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, and <i>Staphylococcus aureus</i>. Then, the cytotoxicity of each substance or mixture against pulp stem cells extracted from 30 primary healthy teeth was evaluated at 4 concentrations. Data were gathered via observation, and optical density values were obtained using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) test and recorded. SPSS software version 23 was used to analyze the data. Data were evaluated using 2-way analysis of variance and the Tukey test.</p><p><strong>Results: </strong>Regarding antimicrobial properties, thyme alone and thyme + propolis had the lowest minimum inhibitory concentrations (MICs) against the growth of <i>S. aureus</i>, <i>E. coli</i>, and <i>P. aeruginosa</i> bacteria. For <i>E. faecalis</i>, thyme + propolis had the lowest MIC, followed by thyme alone. At 24 and 72 hours, thyme + propolis, CEM cement, and propolis had the greatest bioviability in the primary dental pulp stem cells, and lavender + propolis had the lowest bioviability.</p><p><strong>Conclusions: </strong>Of the studied materials, thyme + propolis showed the best results in the measures of practical performance as a dental pulp cap.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"48 1","pages":"e2"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/97/a1/rde-48-e2.PMC9982242.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9412780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of surrounding and underlying shades on the color adjustment potential of a single-shade composite used in a thin layer. 在薄层中使用的单色复合材料的周围和下面的阴影对颜色调整电位的影响。
Restorative Dentistry & Endodontics Pub Date : 2023-02-01 DOI: 10.5395/rde.2023.48.e7
Mariana Silva Barros, Paula Fernanda Damasceno Silva, Márcia Luciana Carregosa Santana, Rafaella Mariana Fontes Bragança, André Luis Faria-E-Silva
{"title":"Effects of surrounding and underlying shades on the color adjustment potential of a single-shade composite used in a thin layer.","authors":"Mariana Silva Barros,&nbsp;Paula Fernanda Damasceno Silva,&nbsp;Márcia Luciana Carregosa Santana,&nbsp;Rafaella Mariana Fontes Bragança,&nbsp;André Luis Faria-E-Silva","doi":"10.5395/rde.2023.48.e7","DOIUrl":"https://doi.org/10.5395/rde.2023.48.e7","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to evaluate the surrounding and underlying shades' effect on the color adjustment potential (CAP) of a single-shade composite used in a thin layer.</p><p><strong>Materials and methods: </strong>Cylinder specimens (1.0 mm thick) were built with the Vittra APS Unique composite, surrounded (dual specimens) or not (simple specimens) by a control composite (shade A1, A2, or A3). Simple specimens were also built only with the control composites. Each specimen's color was measured against white and black backgrounds or the simple control specimens with a spectrophotometer (CIELAB system). The whiteness index for dentistry (WI<sub>D</sub>) and translucency parameters (TP<sub>00</sub>) were calculated for simple specimens. Differences (ΔE<sub>00</sub>) in color between the simple/dual specimens and the controls were calculated. The CAP was calculated based on the ratios between data from simple and dual specimens.</p><p><strong>Results: </strong>The Vittra APS Unique composite showed higher WI<sub>D</sub> and TP<sub>00</sub> values than the controls. The highest values of ΔE<sub>00</sub> were observed among simple specimens. The color measurements of Vittra APS Unique (simple or dual) against the control specimens presented the lowest color differences. Only surrounding the single-shade composite with a shaded composite barely impacted the ΔE<sub>00</sub>. The highest CAP values were obtained using a shaded composite under simple or dual specimens.</p><p><strong>Conclusions: </strong>The CAP of Vittra APS Unique was strongly affected by the underlying shade, while surrounding this composite with a shaded one barely affected its color adjustment.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"48 1","pages":"e7"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bd/5d/rde-48-e7.PMC9982245.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9412777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Appreciation to reviewers and announcement of a new editorial board 感谢审稿人并宣布成立新的编辑委员会
Restorative Dentistry & Endodontics Pub Date : 2023-02-01 DOI: 10.5395/rde.2023.48.e11
Kyung-San Min
{"title":"Appreciation to reviewers and announcement of a new editorial board","authors":"Kyung-San Min","doi":"10.5395/rde.2023.48.e11","DOIUrl":"https://doi.org/10.5395/rde.2023.48.e11","url":null,"abstract":"","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49519096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High-plasticity mineral trioxide aggregate and its effects on M1 and M2 macrophage viability and adherence, phagocyte activity, production of reactive oxygen species, and cytokines. 高弹性三氧化二矿聚集体及其对 M1 和 M2 巨噬细胞活力和粘附性、吞噬细胞活性、活性氧的产生以及细胞因子的影响。
Restorative Dentistry & Endodontics Pub Date : 2022-12-29 eCollection Date: 2023-02-01 DOI: 10.5395/rde.2023.48.e6
Betânia Canal Vasconcellos, Layara Cristine Tomaz Tavares, Danilo Couto da Silva, Francielen Oliveira Fonseca, Francine Benetti, Antônio Paulino Ribeiro Sobrinho, Warley Luciano Fonseca Tavares
{"title":"High-plasticity mineral trioxide aggregate and its effects on M1 and M2 macrophage viability and adherence, phagocyte activity, production of reactive oxygen species, and cytokines.","authors":"Betânia Canal Vasconcellos, Layara Cristine Tomaz Tavares, Danilo Couto da Silva, Francielen Oliveira Fonseca, Francine Benetti, Antônio Paulino Ribeiro Sobrinho, Warley Luciano Fonseca Tavares","doi":"10.5395/rde.2023.48.e6","DOIUrl":"10.5395/rde.2023.48.e6","url":null,"abstract":"<p><strong>Objectives: </strong>This study evaluated the effects of high-plasticity mineral trioxide aggregate (MTA-HP) on the activity of M1 and M2 macrophages, compared to white MTA (Angelus).</p><p><strong>Materials and methods: </strong>Peritoneal inflammatory M1 (from C57BL/6 mice) and M2 (from BALB/c mice) macrophages were cultured in the presence of the tested materials. Cell viability (MTT and trypan blue assays), adhesion, phagocytosis, reactive oxygen species (ROS) production, and tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-β production were evaluated. Parametric analysis of variance and the non-parametric Kruskal-Wallis test were used. Results were considered significant when <i>p</i> < 0.05.</p><p><strong>Results: </strong>The MTT assay revealed a significant decrease in M1 metabolism with MTA-HP at 24 hours, and with MTA and MTA-HP later. The trypan blue assay showed significantly fewer live M1 at 48 hours and live M2 at 48 and 72 hours with MTA-HP, compared to MTA. M1 and M2 adherence and phagocytosis showed no significant differences compared to control for both materials. Zymosan A stimulated ROS production by macrophages. In the absence of interferon-γ, TNF-α production by M1 did not significantly differ between groups. For M2, both materials showed higher TNF-α production in the presence of the stimulus, but without significant between-group differences. Likewise, TGF-β production by M1 and M2 macrophages was not significantly different between the groups.</p><p><strong>Conclusions: </strong>M1 and M2 macrophages presented different viability in response to MTA and MTA-HP at different time points. Introducing a plasticizer into the MTA vehicle did not interfere with the activity of M1 and M2 macrophages.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"48 1","pages":"e6"},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/04/78/rde-48-e6.PMC9982239.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9412779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Apical periodontitis in mesiobuccal roots of maxillary molars: influence of anatomy and quality of root canal treatment, a CBCT study. 上颌磨牙中颊根根尖牙周炎:解剖结构和根管治疗质量的影响:CBCT研究。
Restorative Dentistry & Endodontics Pub Date : 2022-11-01 DOI: 10.5395/rde.2022.47.e37
Samantha Jannone Carrion, Marcelo Santos Coelho, Adriana de Jesus Soares, Marcos Frozoni
{"title":"Apical periodontitis in mesiobuccal roots of maxillary molars: influence of anatomy and quality of root canal treatment, a CBCT study.","authors":"Samantha Jannone Carrion,&nbsp;Marcelo Santos Coelho,&nbsp;Adriana de Jesus Soares,&nbsp;Marcos Frozoni","doi":"10.5395/rde.2022.47.e37","DOIUrl":"https://doi.org/10.5395/rde.2022.47.e37","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to evaluate the prevalence of apical periodontitis (AP) in the mesiobuccal roots of root canal-treated maxillary molars.</p><p><strong>Materials and methods: </strong>One thousand cone-beam computed tomography images of the teeth were examined by 2 dental specialists in oral radiology and endodontics. The internal anatomy of the roots, Vertucci's classification, quality of root canal treatment, and presence of missed canals were evaluated; additionally, the correlation between these variables and AP was ascertained.</p><p><strong>Results: </strong>A total of 1,000 roots (692 first molars and 308 second molars) encompassing 1,549 canals were assessed, and the quality of the root canal filling in the majority (56.9%) of the canals was satisfactory. AP was observed in 54.4% of the teeth. A mesiolingual canal in the mesiobuccal root (MB2 canal) was observed in 54.9% of the images, and the majority (83.5%) of these canals were not filled. Significant associations were observed between the presence of an MB2 canal and the quality of the root canal filling and the presence of AP.</p><p><strong>Conclusions: </strong>AP was detected in more than half of the images. The MB2 canals were frequently missed or poorly filled.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"47 4","pages":"e37"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e7/77/rde-47-e37.PMC9715377.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10362537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Endodontic micro-resurgery and guided tissue regeneration of a periapical cyst associated to recurrent root perforation: a case report. 根管显微手术及引导下组织再生治疗复发性根管穿孔根尖周囊肿1例。
Restorative Dentistry & Endodontics Pub Date : 2022-11-01 DOI: 10.5395/rde.2022.47.e35
Fernando Córdova-Malca, Hernán Coaguila-Llerena, Lucía Garré-Arnillas, Jorge Rayo-Iparraguirre, Gisele Faria
{"title":"Endodontic micro-resurgery and guided tissue regeneration of a periapical cyst associated to recurrent root perforation: a case report.","authors":"Fernando Córdova-Malca,&nbsp;Hernán Coaguila-Llerena,&nbsp;Lucía Garré-Arnillas,&nbsp;Jorge Rayo-Iparraguirre,&nbsp;Gisele Faria","doi":"10.5395/rde.2022.47.e35","DOIUrl":"https://doi.org/10.5395/rde.2022.47.e35","url":null,"abstract":"<p><p>Although the success rates of microsurgery and micro-resurgery are very high, the influence of a recurrent perforation combined with radicular cyst remains unclear. A 21-year-old white female patient had a history of root perforation in a previously treated right maxillary lateral incisor. Analysis using cone-beam computed tomography (CBCT) revealed an extensive and well-defined periapical radiolucency, involving the buccal and palatal bone plate. The perforation was sealed with bioceramic material (Biodentine) in the pre-surgical phase. In the surgical phase, guided tissue regeneration (GTR) was performed by combining xenograft (lyophilized bovine bone) and autologous platelet-rich fibrin applied to the bone defect. The root-end preparation was done using an ultrasonic tip. The retrograde filling was performed using a bioceramic material (Biodentine). Histopathological analysis confirmed a radicular cyst. The patient returned to her referring practitioner to continue the restorative procedures. CBCT analysis after 1-year recall revealed another perforation in the same place as the first intervention, ultimately treated by micro-resurgery using the same protocol with GTR, and a bioceramic material (MTA Angelus). The 2-year recall showed healing and bone neoformation. In conclusion, endodontic micro-resurgery with GTR showed long-term favorable results when a radicular cyst and a recurrent perforation compromised the success.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"47 4","pages":"e35"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2d/0a/rde-47-e35.PMC9715368.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10362540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Evaluation of blood clot, platelet-rich plasma, and platelet-rich fibrin-mediated regenerative endodontic procedures in teeth with periapical pathology: a CBCT study. 评价血凝块、富血小板血浆和富血小板纤维蛋白介导的再生根管治疗在根尖周围病变的牙齿:一项CBCT研究。
Restorative Dentistry & Endodontics Pub Date : 2022-11-01 DOI: 10.5395/rde.2022.47.e41
Swati Markandey, Haridas Das Adhikari
{"title":"Evaluation of blood clot, platelet-rich plasma, and platelet-rich fibrin-mediated regenerative endodontic procedures in teeth with periapical pathology: a CBCT study.","authors":"Swati Markandey,&nbsp;Haridas Das Adhikari","doi":"10.5395/rde.2022.47.e41","DOIUrl":"https://doi.org/10.5395/rde.2022.47.e41","url":null,"abstract":"<p><strong>Objectives: </strong>This study compared the clinical and radiological outcomes of regenerative endodontic procedures (REPs) using blood clots (BCs), platelet-rich plasma (PRP), and platelet-rich fibrin (PRF) through intraoral periapical radiography (IOPAR) and cone-beam computed tomography (CBCT).</p><p><strong>Materials and methods: </strong>Forty-five single-rooted necrotic teeth with periapical pathology were randomly allocated to receive BC, PRP, or PRF as an individual scaffold. Outcomes were evaluated in 35 teeth in 23 patients with a follow-up period of 12-24 months through qualitative IOPAR scoring and quantitative CBCT measurements. Healing of periapical lesions and in immature teeth, changes in the apical foramen diameter (AFD), root wall thickness (RWT), and root length (RL) were assessed. A <i>p</i> value less than 0.05 was considered to indicate statistical significance.</p><p><strong>Results: </strong>All teeth were asymptomatic except 1 in the PRP group. Periapical lesion healing was seen in all except 2 teeth in the BC group and 3 in the PRP group. Both IOPAR and CBCT revealed no significant differences in bone healing or changes in AFD, RWT, and RL among the 3 groups. A positive pulp sensibility response to the cold test was seen in 2 teeth in the BC group, but none to the electric pulp test. Intracanal calcification (ICC) was evident in more teeth in the BC group than in the PRP and PRF groups, and was also significantly higher in immature teeth.</p><p><strong>Conclusions: </strong>Our results revealed that BC, PRP, and PRF have similar potential as scaffolds in REPs, and ICC may be a concern for long-term outcomes.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"47 4","pages":"e41"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/43/8d/rde-47-e41.PMC9715374.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10423633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correlation between different methodologies used to evaluate the marginal adaptation of proximal dentin gingival margins elevated using a glass hybrid. 使用玻璃杂交体提高近端牙本质龈缘边缘适应性的不同评估方法之间的相关性。
Restorative Dentistry & Endodontics Pub Date : 2022-11-01 DOI: 10.5395/rde.2022.47.e36
Hoda S Ismail, Brian R Morrow, Ashraf I Ali, Rabab El Mehesen, Franklin Garcia-Godoy, Salah H Mahmoud
{"title":"Correlation between different methodologies used to evaluate the marginal adaptation of proximal dentin gingival margins elevated using a glass hybrid.","authors":"Hoda S Ismail,&nbsp;Brian R Morrow,&nbsp;Ashraf I Ali,&nbsp;Rabab El Mehesen,&nbsp;Franklin Garcia-Godoy,&nbsp;Salah H Mahmoud","doi":"10.5395/rde.2022.47.e36","DOIUrl":"https://doi.org/10.5395/rde.2022.47.e36","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to evaluate the effect of aging on the marginal quality of glass hybrid (GH) material used to elevate dentin gingival margins, and to analyze the consistency of the results obtained by 3 <i>in vitro</i> methods.</p><p><strong>Materials and methods: </strong>Ten teeth received compound class II cavities with subgingival margins. The dentin gingival margins were elevated with GH, followed by resin composite. The GH/gingival dentin interfaces were examined through digital microscopy, scanning electron microscopy (SEM) using resin replicas, and according to the World Dental Federation (FDI) criteria. After initial evaluations, all teeth were subjected to 10,000 thermal cycles, followed by repeating the same marginal evaluations and energy dispersive spectroscopy (EDS) analysis for the interfacial zone of 2 specimens. Marginal quality was expressed as the percentage of continuous margin at ×200 for microscopic techniques and as the frequency of each score for FDI ranking. Data were analyzed using the paired sample <i>t</i>-test, Wilcoxon signed-rank test, and Pearson and Spearmen correlation coefficients.</p><p><strong>Results: </strong>None of the testing techniques proved the significance of the aging factor. Moderate and strong significant correlations were found between the testing techniques. The EDS results suggested the presence of an ion-exchange layer along the GH/gingival dentin interface of aged specimens.</p><p><strong>Conclusions: </strong>The marginal quality of the GH/dentin gingival interface defied aging by thermocycling. The replica SEM and FDI ranking results had stronger correlations with each other than either showed with the digital microscopy results.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"47 4","pages":"e36"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fa/e1/rde-47-e36.PMC9715370.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10362541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
The influence of sodium hypochlorite concentration on the fibrin structure of human blood clots and transforming growth factor-beta 1 release: an ex vivo study. 次氯酸钠浓度对人血凝块纤维蛋白结构和转化生长因子- β 1释放的影响:体外研究
Restorative Dentistry & Endodontics Pub Date : 2022-11-01 DOI: 10.5395/rde.2022.47.e42
Anisha Mishra, Velmurugan Natanasabapathy, Nandini Suresh
{"title":"The influence of sodium hypochlorite concentration on the fibrin structure of human blood clots and transforming growth factor-beta 1 release: an <i>ex vivo</i> study.","authors":"Anisha Mishra,&nbsp;Velmurugan Natanasabapathy,&nbsp;Nandini Suresh","doi":"10.5395/rde.2022.47.e42","DOIUrl":"https://doi.org/10.5395/rde.2022.47.e42","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the effects of various concentrations of sodium hypochlorite (NaOCl) on human whole-blood clotting kinetics, the structure of the blood clots formed, and transforming growth factor (TGF)-β1 release.</p><p><strong>Materials and methods: </strong>Human whole blood was collected from 5 healthy volunteers and divided into 4 groups: CG (control, 0.5 mL of blood), BN<sub>0.5</sub> (0.5 mL of blood with 0.5 mL of 0.5% NaOCl), BN<sub>3</sub> (0.5 mL of blood with 0.5 mL of 3% NaOCl), and BN<sub>5.25</sub> (0.5 mL of blood with 0.5 mL of 5.25% NaOCl). The effects of NaOCl on clotting kinetics, structure of fibrin and cells, and release of TGF-β1 were assessed using thromboelastography (TEG), scanning electron microscopy (SEM), and enzyme-linked immunosobent assay, respectively. Statistical analysis was conducted using the Kruskal Wallis and Mann-Whitney <i>U</i> tests, followed by the <i>post hoc</i> Dunn test. A <i>p</i> value < 0.05 indicated statistical significance.</p><p><strong>Results: </strong>The blood samples in BN<sub>0.5</sub> and BN<sub>3</sub> did not clot, whereas the TEG of BN<sub>5.25</sub> showed altered clot formation. Samples from the CG and BN<sub>3</sub> groups could only be processed with SEM, which showed that the latter lacked fibrin formation and branching of fibers, as well as clumping of red blood cells with surface roughening and distortion. TGF-β1 release was significantly highest in BN<sub>3</sub> when all groups were compared to CG (<i>p</i> < 0.05).</p><p><strong>Conclusions: </strong>Each concentration of NaOCl affected the release of TGF-β1 from blood clots and altered the clotting mechanism of blood by affecting clotting kinetics and cell structure.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"47 4","pages":"e42"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e0/94/rde-47-e42.PMC9715373.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10362539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Surface gloss, gloss retention, and color stability of 2 nano-filled universal resin composites. 2纳米填充通用树脂复合材料的表面光泽度、保光性和颜色稳定性。
Restorative Dentistry & Endodontics Pub Date : 2022-11-01 DOI: 10.5395/rde.2022.47.e43
Gustavo Fabián Molina, Ricardo Juan Cabral, Ignacio Mazzola, Michael Burrow
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引用次数: 1
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