International Journal of Academic Medicine and Pharmacy最新文献

筛选
英文 中文
Erlotinib and Melatonin Treatment Reduce Proliferation of the Neural Tumor Cells (SH-SY5Y) by Mediating TRPV1 Channel Function 埃洛替尼和褪黑素治疗通过调节TRPV1通道功能减少神经肿瘤细胞(SH-SY5Y)的增殖
International Journal of Academic Medicine and Pharmacy Pub Date : 1900-01-01 DOI: 10.29228/jamp.43598
Ömer Çelik Birol Özkal, İ. Övey, Ö. Çelik
{"title":"Erlotinib and Melatonin Treatment Reduce Proliferation of the Neural Tumor Cells (SH-SY5Y) by Mediating TRPV1 Channel Function","authors":"Ömer Çelik Birol Özkal, İ. Övey, Ö. Çelik","doi":"10.29228/jamp.43598","DOIUrl":"https://doi.org/10.29228/jamp.43598","url":null,"abstract":"Neuroblastoma, which is caused by the precursors of the sympathetic nervous system, is the most commonly encountered extracranial solid tumor in the pediatric age group. TRPV1 channels, which are a subchannel of the TRP channel family in the cell membrane, are channel permeable to calcium that are known to have increased expression based on increased free radicals. They were reported to lead to apoptosis based on increased amount of intracellular free calcium when they are excessively stimulated. With this study, we purposed to determine the effect levels of erlotinib and melatonin on neuroblastoma cells through TRPV1 channels. SH-SY5Y neuroblastoma cells were used in the study. The cell series were divided into 7 main groups as Control, Erlotinib (ERLT) (5 µM, 24 hours), MEL (1 mM, 24 hours), ERLT+CPZ, MEL+CPZ, ERLT+MEL and ERLT+MEL+CPZ. TRPV1 channel activation in all groups was achieved by capsaicin. The calcium signals, intracellular reactive oxygen types, caspase-3 and -9 and mitochondrial membrane depolarization levels were analyzed in the groups, and the groups were compared to each other. Cytosolic calcium concentration, apoptosis, mitochondrial membrane depolarization caspase-3 and caspase-9 activation, and reactive oxygen species levels were importantly higher in comparison to the control group in the ERLT (p<0.001), ERLT+MEL (p<0.001) and MEL (p<0.05; p<0.001) groups. In the comparison of the groups that used TRPV1 channel blocker (ERLT+CPZ, ERLT+MEL+CPZ, MEL+CPZ) and the groups that did not use channel blocker (ERLT, ERLT+MEL, MEL), all values were lower in the group that used the blocker (p<0.05). Using melatonin and erlotinib together in neuroblastoma tumor cells increased apoptotic pathway activity through TRPV1 channels.","PeriodicalId":210978,"journal":{"name":"International Journal of Academic Medicine and Pharmacy","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121544977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Horizontal Augmentation of Alveolar Crest with Onlay Graft: A Case Report 牙槽嵴水平增强的嵌板移植一例报告
International Journal of Academic Medicine and Pharmacy Pub Date : 1900-01-01 DOI: 10.29228/jamp.43459
E. Mavi, Şükrü Kolay
{"title":"Horizontal Augmentation of Alveolar Crest with Onlay Graft: A Case Report","authors":"E. Mavi, Şükrü Kolay","doi":"10.29228/jamp.43459","DOIUrl":"https://doi.org/10.29228/jamp.43459","url":null,"abstract":"Dental implant is an important material used in aesthetic and functional restoration of missing teeth from past to present. However some conditions must be favorable for this treatment. The main one is that the vertical and horizontal volume of the alveolar crest has sufficient height and width to place the dental implant in the proper position. Although many techniques and materials are used while augmenting alveolar crest deficiencies, autogenous grafts remain the gold standard. In this article, we aimed to present the implant placement and prosthetic restoration step by step after the rehabilitation of an inadequate alveolar crest with a corticocancellous block graft taken from the symphysis region of the mandible in a young female patient who lost her teeth due to the failure of the apical resection treatment to the teeth numbered 11 and 21.","PeriodicalId":210978,"journal":{"name":"International Journal of Academic Medicine and Pharmacy","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114941797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信
小红书