{"title":"Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog","authors":"T. Popović, P. Mitrović, A. Kosovac","doi":"10.5937/ratpov58-33227","DOIUrl":"https://doi.org/10.5937/ratpov58-33227","url":null,"abstract":"'Candidates Phytoplasma solani', known by its trivial name stolbur phytoplasma, is a plant pathogen infecting numerous crops in Serbia. Celery plants with prominent leaf yellowing and chlorosis, sporadically with tissue necrosis, were observed during August 2020 in Futog, situated in Novi Sad suburbia in Vojvodina. Total of 12 sampled celery plants, 8 symptomatic and 4 asymptomatic ones, were analysed for 'Ca. P. solani' presence. All symptomatic celery plants were infected with stolbur phytoplasma according to the stoll 1 gene detection and therefore were further subjected to multigene molecular characterization on three genes: tuf, stamp and vmpl. Combining molecular tools PCR/RFLP and sequencing reviled two 'Ca. P. solani' multilocus genotypes in celery: tuf-b/Rqg31/V14 and tuf-b/ Rpm35/V14, present in 4 samples each. Obtained results of the strain genotyping are in concordance with previous data on the 'Ca. P. solani' diversity on celery, but supplemented with genotyping of the vmpl gene. Outbreak of stolbur phytoplasma in the assessed locality in Futog is linked to tuf-b epidemiological cycle correlated in Serbia mainly with weed Convolvulus arvensis which was present in the subjected celery plot, and could have been the phytoplasma inoculum source. Visual evaluation of the symptom occurrence suggests on 10-15% of 'Ca. P. solani' affected celery plants scattered throughout the plot corresponding to the pathogen dispersal in crop by cixiid planthoper Hyalesthes obsoletus (Hemiptera: Auchenorrhyncha) associated with C. arvensis, main vector of stolbur phytoplasma in Serbia.","PeriodicalId":20996,"journal":{"name":"Ratarstvo i Povrtarstvo","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71044567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Radic, I. Balalić, G. Jaćimović, M. Krstić, M. Jocković, S. Jocić
{"title":"A study of correlations and path analyses of some traits in sunflower parental lines","authors":"V. Radic, I. Balalić, G. Jaćimović, M. Krstić, M. Jocković, S. Jocić","doi":"10.5937/RATPOV58-26782","DOIUrl":"https://doi.org/10.5937/RATPOV58-26782","url":null,"abstract":"The trial in this study was carried out in field conditions throughout three years from plots where seed production of sunflower parental lines was established. Eight genotypes were examined; namely RHA-UK, RHA-ST-59, RHA-SES-IMI, RHA-SNRF, RHA-E-ANN-65, RHA-RU-3, RHA-CDN, and RHA-N-M-1. All the examined genotypes are parental components of the best sunflower hybrids developed by the Institute of Field and Vegetable Crops, Novi Sad, Serbia. Relationships between seed yield and eight different traits in sunflower were studied - oil and protein content, seed germination, 1000-seed weight, head diameter, number of branches and leaves and plant height. The same analyses were carried out for comparison of oil content. A highly significant positive correlation was determined between plant height and number of branches; plant height and the number of leaves; plant height and head diameter; plant height and seed yield; number of branches and yield; the number of leaves and yield; head diameter and seed yield; number of leaves and number of branches; number of leaves and head diameter and between seed germination and oil content. Path analysis indicated highest significant direct effect of plant height on yield (0.439**). The number of branches had a significant positive direct effect on yield (0.260*). In the study of indirect effects on yield, thirteen significant effects were determined. The study of direct effects on oil content showed that the plant height and number of leaves diameter had high significant effects (0.800** and -0.847**). The existence of indirect significant effects on oil content was determined in nine cases.","PeriodicalId":20996,"journal":{"name":"Ratarstvo i Povrtarstvo","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71044697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In vitro anther culture as efficiently applied technique for doubled haploid production of wheat (Triticum aestivum L.)","authors":"Osama Kanbar Zuhair, C. Lantos, J. Pauk","doi":"10.5937/RATPOV58-29902","DOIUrl":"https://doi.org/10.5937/RATPOV58-29902","url":null,"abstract":"Acknowledgements: The first author is thankful to Stipendium Hungaricum Scholarship program of the Hungarian government for supporting this work. This research was funded by the Hungarian Academy of Sciences, grant number “János Bolyai Research Scholarship”; National Research, Development and Innovation Office, grant number “OTKA-K_16-K119835”, “GINOP-2.2.1-15-2016-00026”, “GINOP2.2.1-18-2018-00005”, “TUDFO/51757/2019-ITM” and “TKP2020-NKA-21”. The authors thank Elizabeth Búza for the grammatical corrections.","PeriodicalId":20996,"journal":{"name":"Ratarstvo i Povrtarstvo","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71044773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Jankulovska, D. Rajković, D. Miladinović, S. Terzić, N. Grahovac, A. Kondić-Špika, A. Marjanović-Jeromela
{"title":"Classification of rapeseed genotypes based on quantitative traits using multivariate techniques","authors":"M. Jankulovska, D. Rajković, D. Miladinović, S. Terzić, N. Grahovac, A. Kondić-Špika, A. Marjanović-Jeromela","doi":"10.5937/ratpov58-35477","DOIUrl":"https://doi.org/10.5937/ratpov58-35477","url":null,"abstract":"The genetic variability of 13 rapeseed genotypes created in Serbia was assessed at the Institute of Field and Vegetable Crops, Novi Sad, in three consecutive years. The genotypes were evaluated and classified based on 10 quantitative traits (plant height, height to the first branch, stem width, number of lateral branches, number of leaves, number of pods, seed yield per plant, 1000 seed weight, oil content and protein content), using principal component analysis (PCA) and two-way cluster analysis. The first four PCs with eigenvalues >1 contributed to 83% of the total explained variance. The PC analysis revealed that plant height, height to the first branch, number of lateral branches and number of leaves contributed maximum to the total divergence of the material. The genotypes were classified in two main clusters: the first cluster comprised three genotypes, while the other genotypes belonged to the second cluster which can further be divided to two subclusters. One genotype was not classified in these two clusters. The selected genotypes that have desirable traits may be used in hybridization programs for improvement of seed yield and traits that contribute to seed yield most.","PeriodicalId":20996,"journal":{"name":"Ratarstvo i Povrtarstvo","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71045020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Archaeoentomological assessment of weevil (Coleoptera, Bruchidae) infestation level of pea (Pisum sativum) at the Late Bronze Age settlement Hissar","authors":"A. Medović, A. Mikić","doi":"10.5937/RATPOV58-31204","DOIUrl":"https://doi.org/10.5937/RATPOV58-31204","url":null,"abstract":"A find of 2572 charred seeds of pea (Pisum sativum L.) was detected at the Late Bronze Age tell settlement Hissar near Leskovac, in Serbia, belonging to the Brnjica cultural group, 14-10 cent. BC. Two types of pea seeds were observed: apparently healthy seeds and seeds damaged by the activity of a weevil (Coleoptera, Bruchidae). At least two-fifths of all finds have apparently been infested most probably by pea weevil (Bruchus pisorum L.), one of the most important pea pests worldwide, especially in medium-moist and dry climates, such as Southern Europe and Australia. A large amount of infested pea seeds indicates a developed pea production on small plots, strongly indicating that cultivating this ancient pulse crop must have been well-rooted in field conditions. Previous DNA analyses of charred pea placed the ancient Hissar pea at an intermediate position between extantly cultivated pea (P. sativum L. subsp. sativum var. sativum) and a wild, winter hardy, 'tall' pea (P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.). Based on an assumption of its late harvest time and combined with pea weevil life cycle stage in charred seeds, it was possible to estimate the season during which the seeds were carbonized, namely, the second half of July or the first days of August at the latest. Older, final weevil instars were predominant before seed carbonization. The pea infestation rate at Hissar is one of the highest noted among pulses in the Old World and the highest among peas, so far.","PeriodicalId":20996,"journal":{"name":"Ratarstvo i Povrtarstvo","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71044876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Aćimović, A. Varga, M. Cvetković, Pezo Lato, B. Lončar, M. Ignjatov, T. Zeremski
{"title":"Chemical composition of hyssop cv. 'Domaći ljubičasti' essential oil and its antimicrobial activity","authors":"M. Aćimović, A. Varga, M. Cvetković, Pezo Lato, B. Lončar, M. Ignjatov, T. Zeremski","doi":"10.5937/RATPOV58-31751","DOIUrl":"https://doi.org/10.5937/RATPOV58-31751","url":null,"abstract":"Hyssop (Hyssopus officinalis L., Lamiaceae) is a perennial shrub or subshrub violet-blue flowers in verticillasters and spicy taste with a pungent flavour. Besides being used as a culinary herb for flavouring and food preservation, this plant is also an ornamental, bee attracting plant and a traditional remedy for respiratory diseases and digestive disturbances. Hyssop is an essential oil-bearing plant, and its essential oil (Hyssopi aetheroleum) is used in the pharmaceutical, perfume and cosmetics industries as well as in aromatherapy. The objective of this study was to determine the chemical composition of essential oil of hyssop CV. \"Domaći ljubičasti\", grown in Serbia, and investigate its antimicrobial activity against 16 bacteria, mainly pathogens in the food industry. A total of 61 compounds were detected in the hyssop essential oil. The bicyclic monoterpene ketones CIS-pinocamphone (43.8%) and trans-pinocamphone (18.3%) were the most abundant, comprising 62.1%, followed by b-pinene (6.3%) and pinocarvone (6.1%). Hyssop essential oil expressed antibacterial activity against: Staphylococcus aureus, Escherichia coli, Bacillus cereus, Proteus hauseri, Listeria monocytogenes, Rhodococcus equi, Listeria ivanovii, Salmonella Enteritidis, Enterococcus faecalis, Listeria innocua and Bacillus spizizenii. Hyssop essential oil did not express antibacterial activity against Pseudomonas aeruginosa, Salmonella Typhimurium, Klebsiella aerogenes and Staphylococcus epidermidis. Results of this study show that hyssop essential oil has potential for using as natural supplement for control of foodborne diseases of microbiological origin, as well as flavor compositions (herbaceous, camphor-like odour with warm and spicy undernotes), especially for meat products, sauces, soups and seasonings.","PeriodicalId":20996,"journal":{"name":"Ratarstvo i Povrtarstvo","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71044516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Aćimović, B. Lončar, Biljana Kiprovski, Jovana Stankovic-Jeremic, M. Todosijević, L. Pezo, Jelena Jeremić
{"title":"Chamomile essential oil quality after postharvest separation treatments","authors":"M. Aćimović, B. Lončar, Biljana Kiprovski, Jovana Stankovic-Jeremic, M. Todosijević, L. Pezo, Jelena Jeremić","doi":"10.5937/ratpov58-33346","DOIUrl":"https://doi.org/10.5937/ratpov58-33346","url":null,"abstract":"Ecological conditions of the growing areas, growing practices as well as harvest and postharvest processing influence the yield and quality of chamomile. The aim of this research was to determine the influence of chamomile separation methods, as postharvest treatments, on the essential oil quality and content, with a view to improve current primary processing of this valuable medicinal plant. In order to explain the structure of laboratory data that would give deeper assessment of similarities among various samples of chamomile essential oil, PCA was employed. Tested results showed that separation of chamomile plant material, as postharvest and pre-drying treatment, had significant influence on the number of identified compounds in the chamomile essential oil. The highest content of individual essential oil compounds had chamomile flowers with short stems, especially a-bisabolol, chamazulene, Z-spiroether and E-b-famesene. In the essential oil obtained from chamomile flowers with long stems, content of a-bisabolol and chamazulene were significantly lower, while E-b-famesene and Z-spiroether contents were significantly higher. Furthermore, in the essential oil obtained from chamomile plant material without separation, the lowest content of abisabolol and Z-spiroether, and the highest content of E-b-farnesene were recorded. The correlation analysis was performed to investigate the likeness in the amounts of the active compounds of essential oil from differently processed chamomile samples. All these results indicate the importance of precise and controlled postharvest treatments, since it clearly affects the essential oil quality and content in the primary processing of this valuable medicinal plant.","PeriodicalId":20996,"journal":{"name":"Ratarstvo i Povrtarstvo","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71045164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of respiratory activity of albic luvisol contaminated by zinc and nickel during spring rapeseed cultivation","authors":"M. Samardzic, V. I. Andreeva, Z. Galic, J. Vasin","doi":"10.5937/ratpov57-27018","DOIUrl":"https://doi.org/10.5937/ratpov57-27018","url":null,"abstract":"In almost all industrially developed regions of the Russian Federation, the soil is contaminated with heavy metals (HM) often in concentrations which exceed limits of safety. This contamination causes degradation of agricultural land, which underlines the importance of the complex continuous monitoring of the dynamics of on-going changes in urban ecosystems against the background of natural processes. Quantitative assessment of the environmental sustainability of the soil according to the biological indicators of its condition is of enormous importance, in particular the balance of microbial carbon in soils, which are under varying degrees of anthropogenic pressure. Experiments were carried out to determine the phytoremediation potential of the spring rapeseed plants on soil contaminated with heavy metals. The aim of the study was to assess the respiratory activity of albic luvisol at different levels of its contamination with zinc and nickel under the conditions of a growing experiment with spring rapeseed plants. The experimental data on the respiratory activity of soil artificially contaminated with zinc and nickel in the dose range of 400-800 and 30-60 mg kg-1 of soil respectively, showed that microbial activity had a strong positive correlation with the presence of spring rapeseed plants in the vessels and weak correlation on the presence of toxic elements in the soil. According to the respiratory activity of albic luvisol, it was found that in the first 13 days of vegetation, cultivation of spring rapeseed plants had a positive effect on the sustainability of soil microbiocenosis to complex pollution with zinc and nickel.","PeriodicalId":20996,"journal":{"name":"Ratarstvo i Povrtarstvo","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71044635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Belović, A. Torbica, D. Škrobot, J. Tomić, I. Čabarkapa, D. Živančev, Slaviša Štatkić, Vladimir Aćin, K. Kukurová, Z. Ciesarová
{"title":"Potential application of triticale cultivar 'Odisej' for the production of cookies","authors":"M. Belović, A. Torbica, D. Škrobot, J. Tomić, I. Čabarkapa, D. Živančev, Slaviša Štatkić, Vladimir Aćin, K. Kukurová, Z. Ciesarová","doi":"10.5937/ratpov57-24126","DOIUrl":"https://doi.org/10.5937/ratpov57-24126","url":null,"abstract":"","PeriodicalId":20996,"journal":{"name":"Ratarstvo i Povrtarstvo","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71044857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Terzić, A. Marjanović-Jeromela, M. Zorić, V. Sikora, Z. Milovac, P. Mitrović, I. Balalić, V. Radic
{"title":"Effect of organic and conventional farming system and sowing date on yield, seed oil and protein content in rapeseed cultivars","authors":"S. Terzić, A. Marjanović-Jeromela, M. Zorić, V. Sikora, Z. Milovac, P. Mitrović, I. Balalić, V. Radic","doi":"10.5937/ratpov57-23744","DOIUrl":"https://doi.org/10.5937/ratpov57-23744","url":null,"abstract":"Rapeseed is one of the major oil crops, grown in various agroecological conditions. Interest in organic rapeseed is rising, with increasing importance to breeders to determine the need for specific organic breeding programs. The objective of this study was to determine the adaptive value of rapeseed cultivars in organic farming environments. Five winter rapeseed cultivars were grown in conventional and organic plots, each with three sowing dates in four replications. The trials were organized using a randomized block design. The effect of cultivar and farming on emergence, percentage of harvested plants, yield, oil and protein content were investigated. Locally recommended agricultural practices were used to keep the fields free from weeds, insects and diseases. In organic field, weeds were removed mechanically while insects were treated using organic insecticide. The seed samples for analysis of oil and protein content were taken during harvest. Considering agricultural practices, it was found that rapeseed can be successfully grown in organic agriculture, but further improvements are needed to increase stability of production. Early sowing date provides enough time for rapeseed growth before the winter and good overwintering. The cultivars had higher oil content in the conventional farming, while there was no significant effect of farming system on protein content. For cultivar Slavica, higher yield was recorded in organic system, while cultivars Banacanka and Nena had high yield in both farming systems. The results suggest that the existing conventional breeding material can be used as a good starting point for further trait improvements in organic farming.","PeriodicalId":20996,"journal":{"name":"Ratarstvo i Povrtarstvo","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71044178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}