Rejuvenation research最新文献_第6页

Antiaging Vaccines Targeting Senescent Cells. 针对衰老细胞的抗衰老疫苗

IF 2.6 4区医学

Rejuvenation research Pub Date : 2022-02-01 DOI: 10.1089/rej.2022.0008

Andrew R Mendelsohn, James W Larrick

{"title":"Antiaging Vaccines Targeting Senescent Cells.","authors":"Andrew R Mendelsohn, James W Larrick","doi":"10.1089/rej.2022.0008","DOIUrl":"https://doi.org/10.1089/rej.2022.0008","url":null,"abstract":"The development of senomorphic drugs to attenuate the senescent phenotype and senolytics to clear pro-inflammatory senescent cells (SCs) to treat aging-associated disorders is being hotly pursued. The effort is complicated by the fact that SCs play a constructive role in some cellular processes such as tissue repair and wound healing. However, concerns about efficacy, which SCs to target, and unwanted side effects have created potential roadblocks. Chimeric antigen receptor T cells directed against urokinase-type plasminogen activator receptor, which is expressed on at least a subset of SCs in atherosclerotic plaques and fibrotic livers, removed SC and improved glucose metabolism. A vaccine targeting CD153-expressing senescent T cells also improved glucose metabolism in obese mice. Recent work to selectively target SCs associated with several pathologies has resulted in the creation of a peptide vaccine that primarily targets endothelial cells expressing high levels of GPNMB, recently identified as a biomarker of senescence. The vaccine reduces atherosclerotic plaque burden and metabolic dysfunction such as glucose intolerance in mouse models of obesity and atherosclerosis. For translation to humans the activity of the vaccine will need to be tightly controlled, as the target GPNMB has multiple roles in normal physiology, including acting to inhibit and possibly resolve inflammation. A promising alternative approach would be to use passive immunization with a monoclonal antibody directed against GPNMB.","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":"25 1","pages":"39-45"},"PeriodicalIF":2.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39723353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Platelet Total PLA₂ Activity, Serum Oxidative Level, and Plasma Cu/Zn Ratio: A Vicious Cycle with a Potential Role to Monitor MCI and Alzheimer's Disease Progression. 血小板总PLA2活性、血清氧化水平和血浆铜/锌比:监测MCI和阿尔茨海默病进展的潜在作用的恶性循环

IF 2.6 4区医学

Rejuvenation research Pub Date : 2022-02-01 DOI: 10.1089/rej.2021.0020

Marta Balietti, Tiziana Casoli, Robertina Giacconi, Cinzia Giuli

{"title":"Platelet Total PLA2 Activity, Serum Oxidative Level, and Plasma Cu/Zn Ratio: A Vicious Cycle with a Potential Role to Monitor MCI and Alzheimer's Disease Progression.","authors":"Marta Balietti, Tiziana Casoli, Robertina Giacconi, Cinzia Giuli","doi":"10.1089/rej.2021.0020","DOIUrl":"https://doi.org/10.1089/rej.2021.0020","url":null,"abstract":"Alzheimer's disease (AD) has no cure, mainly because of late diagnosis. Early diagnostic biomarkers are crucial. Phospholipases A2 (PLA2) are hydrolases with several functions in the brain, nevertheless their deregulation contributes to neurodegeneration. We evaluated platelet total PLA2 activity (ptotPLA2) in healthy elderly subjects (HE, n = 102), patients suffering from mild cognitive impairment (MCI, n = 90) and AD (n = 91). Platelets are considered \"circulating neurons\" and ptotPLA2 appears to mirror the cerebral activity. ptotPLA2 of the three cohorts was similar, but in MCI the higher ptotPLA2 the worse the global cognitive status (Mini Mental State Examination score [MMSE]) and in AD the lower ptotPLA2 the more severe the pathology stage (Clinical Dementia Rating [CDR]). Accordingly, MCI with MMSE ≥26 overlapped HE, in MCI with MMSE <26 and in AD with CDR 1 ptotPLA2 increased, in AD with CDR 2 ptotPLA2 decreased. In MCI ptotPLA2 positively correlated with blood oxidation and inflammation, in AD it was the opposite. Finally, Discrimination Index (DI)-calculated multiplying ptotPLA2, oxidative level and Cu/Zn ratio (an inflammation parameter)-differentiated MCI patients who progressed to dementia in the following 24 months and AD patients with the worse pathology development. Summarizing, ptotPLA2 changes during MCI and AD progression, is linked, in opposite way, to oxidative/inflammatory status in MCI and AD and might help, when included in DI, to identify MCI converters to dementia and AD patients with the more severe prognosis. ptotPLA2 may have a diagnostic/prognostic value and be a potential therapeutic target.","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":"25 1","pages":"16-24"},"PeriodicalIF":2.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39818791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Correlation Between Telomere Length and Biomarkers of Oxidative Stress in Human Aging. 人类衰老过程中端粒长度与氧化应激生物标志物的相关性

IF 2.6 4区医学

Rejuvenation research Pub Date : 2022-02-01 Epub Date: 2022-02-03 DOI: 10.1089/rej.2021.0045

Somu Yadav, Pawan Kumar Maurya

{"title":"Correlation Between Telomere Length and Biomarkers of Oxidative Stress in Human Aging.","authors":"Somu Yadav, Pawan Kumar Maurya","doi":"10.1089/rej.2021.0045","DOIUrl":"https://doi.org/10.1089/rej.2021.0045","url":null,"abstract":"The telomere length (TL) has increasingly been used as a biomarker of human aging because it has been shown to predict the chances of survival and longevity. Oxidative stress is presumed to be a major cause of telomere shortening, but the importance of oxidative stress as a determinant of telomere shortening remains less clear and has recently been questioned. We analyzed 105 healthy subjects of both sexes between the ages of 20-77 years. The TL and biomarkers of oxidative stress were estimated as per standard protocols. A significant (p < 0.001) age-dependent decline in TL was observed. TL was positively correlated with the ferric reducing ability of plasma value (r = 0.8811) and reduced glutathione (r = 0.8209), whereas negatively correlated with malondialdehyde (r = -0.7191). Our findings supported the idea of a possible correlation between the TL and biomarkers of oxidative stress in aging. The study has remarkable scope in medical science as the findings on correlation of TL with biomarkers of oxidative stress in aging are novel and they will help in further research against oxidative stress.","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":"25 1","pages":"25-29"},"PeriodicalIF":2.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39833266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validated Living Worldwide Supercentenarians 113+, Living and Recently Deceased: February 2022. 世界范围内确认的113岁以上的超级百岁老人，在世和最近去世:2022年2月。

IF 2.6 4区医学

Rejuvenation research Pub Date : 2022-02-01 DOI: 10.1089/rej.2022.0011

Robert D Young

引用次数: 0

Acknowledgment of Reviewers 2021. 2021年评审员致谢。

IF 2.6 4区医学

Rejuvenation research Pub Date : 2022-02-01 DOI: 10.1089/rej.2021.29004.ack

引用次数: 0

In Vivo Supportive Effects of Mesenchymal Stem Cells on Fat Graft Stabilization and Local Induction of Angiogenesis Are Not Dependent on the Cell Donor Age or In Vitro Cell Culture Duration. 间充质干细胞对脂肪移植稳定和局部血管生成的体内支持作用不依赖于细胞供体年龄或体外细胞培养时间。

IF 2.6 4区医学

Rejuvenation research Pub Date : 2021-12-01 DOI: 10.1089/rej.2021.0042

Katarzyna Siennicka, Paweł Piotrowski, Wojciech Olszewski, Marta Gajewska, Sławomir Mazur, Zygmunt Pojda

{"title":"In Vivo Supportive Effects of Mesenchymal Stem Cells on Fat Graft Stabilization and Local Induction of Angiogenesis Are Not Dependent on the Cell Donor Age or In Vitro Cell Culture Duration.","authors":"Katarzyna Siennicka, Paweł Piotrowski, Wojciech Olszewski, Marta Gajewska, Sławomir Mazur, Zygmunt Pojda","doi":"10.1089/rej.2021.0042","DOIUrl":"https://doi.org/10.1089/rej.2021.0042","url":null,"abstract":"Mesenchymal stromal cells from adipose tissue (adipose stromal cells, ASCs) are regulators of repair processes in situ by paracrine mechanisms. These unique capabilities make ASCs candidates for the regenerative medicine applications, including cell-assisted lipotransfer method. ASC aging processes have been extensively researched in vitro, there is however limited information about the impact of ASC aging on their biological role in tissue regeneration in vivo. The aim of our study was the research of the possible effects of aging processes of ASCs resulting from the donor age or from in vitro aging during long-term culture (ASC expansion in bioreactors) on their capability to support survival of adipose subcutaneous transplants in rats. The supportive in vivo effects of ASCs from young donors were compared with the effects of ASCs from old donors and ASCs \"aged\" in long-term in vitro cultures. Fat grafts enriched with ASCs (regardless of their age) retain their volume longer than fat grafts without ASCs supplementation. Vascular expansion in cell-enriched fat grafts was more intense when compared with the controls. It may be concluded that the aging of ASCs does not substantially reduce their ability for the support of the survival of adipose tissue grafts.","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":"24 6","pages":"441-448"},"PeriodicalIF":2.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39734489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stem Cell Rejuvenation by Restoration of Youthful Metabolic Compartmentalization. 干细胞年轻化的恢复代谢区隔化。

IF 2.6 4区医学

Rejuvenation research Pub Date : 2021-12-01 DOI: 10.1089/rej.2021.0076

Andrew R Mendelsohn, James W Larrick

{"title":"Stem Cell Rejuvenation by Restoration of Youthful Metabolic Compartmentalization.","authors":"Andrew R Mendelsohn, James W Larrick","doi":"10.1089/rej.2021.0076","DOIUrl":"https://doi.org/10.1089/rej.2021.0076","url":null,"abstract":"Stem cell dysfunction is a hallmark of aging. Much recent study suggests that epigenetic changes play a critical role in the loss of stem cell function with age. However, the underlying mechanisms require elucidation. A recent report describes a process by which mild mitochondrial stress associated with aging causes lysosomal-mediated decreases in CiC, the mitochondrial citrate transporter, in bone marrow-derived mesenchymal stem cells (MSCs). This, in turn, results in a deficit of acetyl-CoA in the nucleus and hypoacetylation of histones. The altered epigenome results in skewered stem cell differentiation favoring adipogenesis and disfavoring osteogenesis, which is problematic given the role the MSCs play in maintaining the integrity of bone tissue. Restoration of nuclear acetyl-CoA by either ectopic expression of CiC or acetate supplementation of MSCs in culture rejuvenates the MSC, restoring the potential to efficiently differentiate along the osteogenic lineage. Citrate, which has recently been reported to extend lifespan in Drosophila, chemically incorporates acetyl-CoA and may prove useful to restore cytoplasmic and nuclear acetyl-CoA levels. The general applicability of the CiC defect in old cells, particularly stem cells, should be established.","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":"24 6","pages":"470-474"},"PeriodicalIF":2.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39677887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Commentary on Some Recent Theses Relevant to Combating Aging: December 2021. 最近一些与抗衰老有关的论文评论:2021年12月。

IF 2.6 4区医学

Rejuvenation research Pub Date : 2021-12-01 DOI: 10.1089/rej.2021.0079

Benjamin Zealley

引用次数: 1

Using Modified Skin-Stretching Technique as an Alternative Solution for the Closure of Moderate and Extensive Skin Defects. 改良皮肤拉伸技术作为修复中、大面积皮肤缺损的替代方法。

IF 2.6 4区医学

Rejuvenation research Pub Date : 2021-12-01 Epub Date: 2021-11-16 DOI: 10.1089/rej.2020.2389

Xin Wang, Yixin Zhang, Sally Kiu-Huen Ng, Zheng Zhang, ZheMing Pu, Huilin Yang, PeiRu Min

{"title":"Using Modified Skin-Stretching Technique as an Alternative Solution for the Closure of Moderate and Extensive Skin Defects.","authors":"Xin Wang, Yixin Zhang, Sally Kiu-Huen Ng, Zheng Zhang, ZheMing Pu, Huilin Yang, PeiRu Min","doi":"10.1089/rej.2020.2389","DOIUrl":"https://doi.org/10.1089/rej.2020.2389","url":null,"abstract":"External skin-stretching devices have been developed and used for wound closure since 1970s. Devices such as Miami STAR®, SureClosure®, TopClosure®, and WiseBand® have their own advantages and disadvantages. The modified external skin-stretching technique of this case series study has the advantage to improve tension distribution and simplified the application. Between January 2014 and June 2017, 20 patients were treated with the modified external skin-stretching device for the closure of the skin defects of the trunk (n = 6) and extremities (n = 14). Skin defects ranged from 8 × 5 to 19 × 16 cm achieved primary closure with the utilization of the modified skin-stretching device without major complications. Subsequent minor revisions were performed under local anesthesia between 6 and 12 months postoperatively. The modified skin-stretching device utilized biomechanical properties and mechanical creep of skin tissue to achieve a reliable and effective primary closure for moderate to extensive skin defects. Therefore, this modified external skin-stretching technique provided, in the appropriate setting, an effective alternative to skin grafts or free flaps.","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":"24 6","pages":"407-416"},"PeriodicalIF":2.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39662043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Fluorescence-Based Detection of Ferrous Iron in Senescent Cells. 荧光法检测衰老细胞中的亚铁。

IF 2.6 4区医学

Rejuvenation research Pub Date : 2021-12-01 DOI: 10.1089/rej.2021.0075

Kyle J Parella, Charles Manhardt, Danny Capucilli, Brandon Moyer, Hanna Colegrove, Kelsey J Moody, Meegan Sleeper, Andrew Banas, Abdelhadi Rebbaa, Aaron J Wolfe

{"title":"Fluorescence-Based Detection of Ferrous Iron in Senescent Cells.","authors":"Kyle J Parella, Charles Manhardt, Danny Capucilli, Brandon Moyer, Hanna Colegrove, Kelsey J Moody, Meegan Sleeper, Andrew Banas, Abdelhadi Rebbaa, Aaron J Wolfe","doi":"10.1089/rej.2021.0075","DOIUrl":"https://doi.org/10.1089/rej.2021.0075","url":null,"abstract":"A major limitation in aging research is the lack of reliable biomarkers to assess phenotypic changes with age or monitor response to antiaging interventions. This study investigates the role of intracellular ferrous iron (Fe2+) as a potential biomarker of senescence. Iron is known to accumulate in various tissues with age and recent studies have demonstrated that its level increases dramatically in senescent cells. The current techniques used to measure the accumulation of iron are cumbersome and only measure total iron not specific isotopes such as the redox reactive Fe2+. It is still to be determined whether the damaging form of iron (Fe2+) is specifically elevated in senescent cells. In this study, we assessed the potential use of a newly discovered Fe2+ reactive probe (SiRhoNox-1) for selective labeling of senescent cells in vitro. For this we have generated various senescent cell models and subjected them to SiRhoNox-1 labeling. Our results indicate that SiRhoNox-1 selectivity labels live senescent cells and was more specific and faster than current staining such as SA-βGal or a derived fluorescent probe C12FDG. Together these findings suggest that SiRhoNox-1 may serve as a convenient tool to detect senescent cells based on their ferrous iron level.","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":"24 6","pages":"456-463"},"PeriodicalIF":2.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39674939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2