Reproductive and Developmental Medicine最新文献

{"title":"Evaluation of fetoplacental oxygenation in a rat model of gestational diabetes mellitus using BOLD-MRI at 7.0-T","authors":"Ying-Fang Wang, Chang-An Chen, Ming-qing Li, Xiang Tao, Xiangtao Zhao, Cheng-jie Wang, Guofu Zhang, He Zhang","doi":"10.1097/RD9.0000000000000062","DOIUrl":"https://doi.org/10.1097/RD9.0000000000000062","url":null,"abstract":"Objective: To compare the differences in blood oxygen level-dependent (BOLD) parameters following maternal hyperoxia between normal pregnancy and pregnancy in the rat model of gestational diabetes mellitus (GDM). Methods: GDM was induced by high-fat and sucrose diet (HFS) combined with an intraperitoneal injection of streptozotocin (STZ). On embryonic day 19 (E19), the two groups of pregnant rats were imaged using a 7.0-T animal MRI scanner. TurboRARE was initially used to localize the fetoplacental units (FPUs). Next, multiple gradient echo BOLD was performed during the air and oxygen inhalation periods. T2* map was then generated, and the baseline T2* and absolute changes in T2* value (ΔT2*, difference between T2*oxy and T2*air) were calculated. Following the MRI scan, the placentas and fetuses were aseptically stripped, weighed, and immunostained. Results: Nine rats were used in this study. After maternal oxygen inhalation, T2* increased significantly in all subjects in both groups. The ΔT2* for the placenta (5.97 vs. 7.81 msec; P = 0.007) and fetal brain (2.23 vs. 3.97 msec; P = 0.005) differed significantly between the GDM and control groups. Histochemical detection of placental glycogen content and inflammatory cytokines (IL-6 and TNF-α) showed significantly higher levels in the GDM than in the normal placenta. Conclusions: BOLD-MRI revealed abnormalities in the fetoplacental response to maternal hyperoxygenation in rats with GDM. We believe that this approach can potentially be used to evaluate placental dysfunction and assess the state of the fetus during pregnancy with GDM.","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":"7 1","pages":"88 - 95"},"PeriodicalIF":0.8,"publicationDate":"2023-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43005883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Executive Editor-in-Chief’s introduction for This Special Issue","authors":"Heqing Huang","doi":"10.1097/rd9.0000000000000060","DOIUrl":"https://doi.org/10.1097/rd9.0000000000000060","url":null,"abstract":"","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44281445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of assisted reproductive technology on perinatal outcomes and offspring health","authors":"Hong Jin, Yi-juan Sun, Yi-Min Shu","doi":"10.1097/RD9.0000000000000057","DOIUrl":"https://doi.org/10.1097/RD9.0000000000000057","url":null,"abstract":"Assisted reproductive technology (ART) has evolved rapidly over the last 40 years, offering hope to individuals and couples struggling with infertility. As technology continues to evolve, simulating a realistic female reproductive system environment has become a common goal for all types of ARTs, thereby reducing the impact of the artificial microenvironment on perinatal and offspring health. In this review, we provide a brief history of the development of each major ART and discuss the impact of ART on perinatal and offspring health. We also explore how the negative consequences of ART may be overcome and how its benefits can be maximized.","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":"7 1","pages":"56 - 63"},"PeriodicalIF":0.8,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"61786285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Executive Editor-in-Chief’s introduction for This Special Issue","authors":"W. Yeung","doi":"10.1097/rd9.0000000000000050","DOIUrl":"https://doi.org/10.1097/rd9.0000000000000050","url":null,"abstract":"","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43593419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of lncRNA-miRNA-mRNA networks in late-onset pre-eclampsia","authors":"Yao Tang, Zhen-Zhen Liu, Hai-yan Liu, Cheng-jie Wang, J. Pei, Nan Chu, T. Peng, Xiao-tian Li, W. Gu","doi":"10.1097/RD9.0000000000000056","DOIUrl":"https://doi.org/10.1097/RD9.0000000000000056","url":null,"abstract":"Objective: Long non-coding RNAs (lncRNAs) are implicated in multiple pathophysiological processes in placenta-related disorders; however, their expression and function in late-onset pre-eclampsia (LOPE) remain unclear. This study aimed to investigate the expression of lncRNAs in LOPE, construct a competing endogenous RNA (ceRNA) network, and identify the pathways associated with LOPE pathogenesis. Methods: We performed lncRNA and mRNAs microarray profiling to identify the differential expression profiles of lncRNAs and mRNAs in LOPE compared to those in normal pregnancy. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to validate differentially expressed genes. Subsequently, we generated an interaction network between lncRNAs, (micro-RNAs) miRNAs, and mRNAs based on the Pearson’s correlation coefficient between lncRNAs and mRNAs. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed to understand the functional significance of differentially expressed lncRNAs (DElncRNAs) in LOPE. Results: We identified 29 DElncRNAs (25 upregulated and four downregulated) and 212 differentially expressed mRNAs (DEmRNAs; 203 upregulated and nine downregulated) in LOPE placentas. Within them, six lncRNAs and four mRNAs were verified by qRT-PCR. GO and KEGG analyses revealed the potential pathways affected by these mRNAs, such as positive regulation of leukocyte chemotaxis, chemokine signaling pathway, and response to hypoxia. Finally, we constructed a ceRNA network including three DElncRNAs and 124 DEmRNAs, whose competing interactions may be mediated by 17 miRNAs. Two DElncRNAs, ENST00000515376 and ENST00000520544, were found to be hub genes, as they interacted with most miRNAs and mRNAs. ENST00000515376 is most likely related to the metabolic process of arachidonic acid, whereas ENST00000520544 is more likely related to the coagulation system, such as the regulation of blood coagulation and platelet degranulation. Conclusion: Differential expression profile of lncRNAs and the lncRNA-miRNA-mRNA network in LOPE provide potential therapeutic targets for this disease.","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":"7 1","pages":"68 - 74"},"PeriodicalIF":0.8,"publicationDate":"2022-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45810936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying miRNA biomarkers of polycystic ovary syndrome through text mining","authors":"Y. Wang, Xiaoyin Yuan, Bin Zhao","doi":"10.1097/RD9.0000000000000055","DOIUrl":"https://doi.org/10.1097/RD9.0000000000000055","url":null,"abstract":"Objective: Polycystic ovary syndrome (PCOS) is an endocrine disorder with diverse clinical manifestations that often occurs in women of childbearing age. However, its molecular pathogenesis remains unclear, and this study aimed to identify miRNA targets in PCOS through text mining and database analysis. Methods: First, three different sets of text mining genes (TMGs) associated with “polycystic ovary syndrome”, “obesity/adiposis”, and “anovulation” keywords were retrieved from the GenCLiP3 database, and overlapping genes were selected. Second, Gene ontology annotation and biological pathway enrichment analyses of these overlapping TMGs were performed, followed by protein–protein interaction (PPI) network analysis. Third, genes in the gene module clustered in the PPI were selected to predict potential miRNAs for PCOS via miRNA-mRNA analysis. Results: A total of 4291 TMGs related to three different keywords were obtained through text mining; 72 intersect TMGs were retained among the three gene sets, and 62 TMGs participated in the establishment of the PPI network, of which 18 were aggregated in the gene module. Finally, 11 miRNAs that simultaneously bound to two TMGs (IGF1, ESR1, MAPK1, NAMPT, PIK3CA, and SERPINE1) could be prioritized as targets to study PCOS. Conclusion(s): The discovery of 11 miRNAs (miR-301a-3p, miR-301b-3p, miR-3666, miR-454-3p, miR-130a-3p, miR-130b-3p, miR-4295, miR-190a-3p, miR-5011-5p, miR-548c-3p, and miR-4799-5p) and 6 TMGs, which are associated with the HIF-1 signaling pathway (P = 4.799E-08), could be used as potential targets for PCOS.","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":"7 1","pages":"96 - 101"},"PeriodicalIF":0.8,"publicationDate":"2022-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42468281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of symptomatic late-onset hypogonadism and lower urinary tract symptoms in aging males: a community-based study","authors":"Guoqing Liang, Jian-Hui Li, H. Shi, Junbiao Zheng, Xiaohua Yu, Shucheng Zhang, Zheng Li, Qian-xi Zhu, Yuxuan Song, Feng Jiang, Yong Zhu","doi":"10.1097/RD9.0000000000000054","DOIUrl":"https://doi.org/10.1097/RD9.0000000000000054","url":null,"abstract":"Objective: Testosterone deficiency may be a risk factor for lower urinary tract symptoms (LUTS), and there may be a causal link between the emergence of LUTS and the incidence of late-onset hypogonadism (LOH). We performed an epidemiologic study to investigate the association between symptomatic late-onset hypogonadism (SLOH) and LUTS in middle-aged and elderly rural Chinese males. Methods: A total of 965 men completed a questionnaire and underwent a detailed physical examination. The Aging Males’ Symptoms (AMS) scale was used to assess SLOH, and the International Prostate Symptom Score (IPSS) questionnaire was used to assess LUTS. Serum reproductive hormone levels of testosterone, sex hormone-binding globulin (SHBG) and luteinizing hormone (LH) were measured. Results: A total of 965 males (mean age: 56.34 ± 8.85, range: 40–80 years) were recruited for the present study. A total of 20.93% (202/965) were diagnosed with SLOH. A total of 93.16% (899/965) had mild LUTS, 5.18% (50/965) had moderate LUTS, and 1.66% (16/965) had severe LUTS. Among SLOH patients, 13.40% (27/202) and 3.90% (8/202) had moderate and severe LUTS, respectively. Patients with severe LUTS had increased SHBG and LH compared with those with mild and moderate LUTS (P <0.01). Correlation analysis revealed that the AMS total score was positively correlated with the IPSS score (P <0.05). The prevalence of SLOH was significantly increased with LUTS severity. In addition to the known effect of age, the results of multiple regression analysis also showed that serum LH or SHBG appeared to have a weak link with SLOH and LUTS that requires etiological and biological clarification in our future study. Conclusion: In this cross-sectional analysis of SLOH and LUTS, LUTS severity was significantly associated with hypogonadism symptoms. Additionally, the prevalence of SLOH advanced with increasing LUTS severity. Serum SHBG or LH showed a positive correlation with SLOH and LUTS.","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":"7 1","pages":"129 - 134"},"PeriodicalIF":0.8,"publicationDate":"2022-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42710208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Focal adhesion kinase signaling is necessary for the hydrogen sulfide-enhanced proliferation, migration, and invasion of HTR8/SVneo human trophoblasts","authors":"Xian-Li Wang, Jing Tang","doi":"10.1097/RD9.0000000000000047","DOIUrl":"https://doi.org/10.1097/RD9.0000000000000047","url":null,"abstract":"Objective: Hydrogen sulfide (H2S) has been elucidated that it promotes migration and invasion in human placenta trophoblasts. However, the signaling pathway underlying H2S-based regulation of trophoblasts remains unknown. Hence, we investigated the potential effect of sodium hydrosulfide (NaHS), an exogenous H2S donor, on extravillous trophoblasts. Methods: The Cell Counting Kit-8 was used to detect the proliferative activity of trophoblasts and to screen the optimal concentration of NaHS. The migration and invasion of HTR8/SVneo cells were measured by Transwell assays. Gene expression was determined by quantitative real-time PCR analysis. Protein expression was determined by western blot. Results: We found that NaHS could promote the proliferation, migration, and invasion of HTR8/SVneo cells. The phosphorylation of focal adhesion kinase (FAK), Src, and extracellular signal-regulated kinase (ERK) were activated by NaHS. Moreover, NaHS also upregulated the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9, downregulated the expression of E-cadherin in HTR8/SVneo cells. The application of NaHS could increase the expression of cystathionine-β-synthase. Conclusion: Both FAK–Src signaling and the upstream signaling cascade of ERK activation play a significant important role in NaHS-induced proliferation, migration, and invasion via upregulating activity of MMP-2, MMP-9, and downregulating E-cadherin in HTR8/SVneo cells. These novel findings may provide a strong foundation for the clinical application of H2S donor drugs.","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":"7 1","pages":"75 - 82"},"PeriodicalIF":0.8,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49548509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of COVID-19 infection during pregnancy on maternal and fetal outcomes","authors":"M. Anter, Nasser Abd El-Aal, M. Rezk, Hussein Fahmy Moawad, A. Abudakika","doi":"10.1097/RD9.0000000000000044","DOIUrl":"https://doi.org/10.1097/RD9.0000000000000044","url":null,"abstract":"Objectives: The objective of this study is to assess the impact of coronavirus disease 2019 (COVID-19) infection during pregnancy on maternal and fetal outcome in relation to gestational age. Methods: This retrospective study was conducted between May 2020 and July 2021. Sixty-five women infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and hospitalized at a quarantine hospital were included. Thirty-four women received prenatal care at the hospital until delivery, while 31 dropped out from the study due to COVID-19 recovery and discharge before delivery. Results: COVID-19 was found in 83% of the pregnant women after 20 weeks of pregnancy. The most common symptoms were cough and fever. Medical complications included severe pneumonia and thrombosis. The maternal mortality rate was 9.2%. Premature rupture of membranes and severe hypertension during labor affected nearly 9% of the pregnant women who completed prenatal care until delivery at the hospital. Preterm labor occurred at a rate of 38.2%. There were seven (20.8%) stillbirths and two cases of infant mortality. Conclusion: Hospitalized pregnant women who have coronavirus infection, lymphopenia, and a high C-reactive protein level were at a higher risk of developing severe illness, which can lead to maternal and neonatal complications.","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":"7 1","pages":"108 - 114"},"PeriodicalIF":0.8,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45230069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of pre-implantation genetic testing technologies and applications","authors":"Ren-Qian Du, D. Zhao, Kai Kang, Fan Wang, R. Xu, Chun-Li Chi, L. Kong, B. Liang","doi":"10.1097/RD9.0000000000000049","DOIUrl":"https://doi.org/10.1097/RD9.0000000000000049","url":null,"abstract":"The first practice of pre-implantation genetic testing (PGT) was reported more than 30 years ago. PGT, originally named pre-implantation genetic screening (PGS) and pre-implantation genetic diagnosis (PGD), is now categorized as PGT for aneuploidies (PGT-A), PGT for monogenic/single-gene defects (PGT-M), and PGT for chromosomal structural rearrangements (PGT-SR). Patients with fertility issues caused by advanced maternal age, carrier status of chromosomal abnormalities, or harboring pathogenic variant(s) are recommended to undergo PGT to increase the possibility of successful live birth and avoid potentially affected newborns. High-throughput techniques, such as DNA microarrays and next-generation sequencing (NGS), have enabled comprehensive screening of all 24 chromosomes, instead of few loci at a time. Furthermore, as a comprehensive PGT, PGT-Plus was enabled by the rapid development of a genome-wide single-cell haplotyping technique to detect embryo aneuploidy, single-gene disorders, and chromosomal aberrations simultaneously using a single universal protocol. In addition, non-invasive approaches enable a more intact embryo during the biopsy procedure, which may avoid potential mosaicism issues at a certain scale by testing spent culture media (SCM). As a novel PGT application, PGT-P detects genome-wide variations in polygenic diseases, which account for a large proportion of premature human deaths and affect a markedly larger population than monogenic diseases, using polygenic risk score calculation to decrease the potential of affecting complex conditions. Owing to the emergence of new technologies recruited to PGTs, more couples with infertility issues have a promising chance of conceiving a healthy baby, ultimately facilitating the human species to live more prosper.","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":"7 1","pages":"20 - 31"},"PeriodicalIF":0.8,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48336743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}