Regenerative Engineering and Translational Medicine最新文献

{"title":"Advancement of Organoid Technology in Regenerative Medicine.","authors":"Babak Arjmand,&nbsp;Zahra Rabbani,&nbsp;Faezeh Soveyzi,&nbsp;Akram Tayanloo-Beik,&nbsp;Mostafa Rezaei-Tavirani,&nbsp;Mahmood Biglar,&nbsp;Hossein Adibi,&nbsp;Bagher Larijani","doi":"10.1007/s40883-022-00271-0","DOIUrl":"https://doi.org/10.1007/s40883-022-00271-0","url":null,"abstract":"<p><strong>Purpose: </strong>Organoids are three-dimensional cultures of stem cells in an environment similar to the body's extracellular matrix. This is also a novel development in the realm of regenerative medicine. Stem cells can begin to develop into 3D structures by modifying signaling pathways. To form organoids, stem cells are transplanted into the extracellular matrix. Organoids have provided the required technologies to reproduce human tissues. As a result, it might be used in place of animal models in scientific study. The key goals of these investigations are research into viral and genetic illnesses, malignancies, and extracellular vesicles, pharmaceutical discovery, and organ transplantation. Organoids can help pave the road for precision medicine through genetic editing, pharmaceutical development, and cell therapy.</p><p><strong>Methods: </strong>PubMed, Google Scholar, and Scopus were used to search for all relevant papers written in English (1907-2021). The study abstracts were scrutinized. Studies on the use of stem-cell-derived organoids in regenerative medicine, organoids as 3D culture models for EVs analysis, and organoids for precision medicine were included. Articles with other irrelevant aims, meetings, letters, commentaries, congress and conference abstracts, and articles with no available full texts were excluded.</p><p><strong>Results: </strong>According to the included studies, organoids have various origins, types, and applications in regenerative and precision medicine, as well as an important role in studying extracellular vesicles.</p><p><strong>Conclusion: </strong>Organoids are considered a bridge that connects preclinical studies to clinical ones. However, the lack of a standardized protocol and other barriers addressed in this review, hinder the vast use of this technology.</p><p><strong>Lay summary: </strong>Organoids are 3D stem cell propagations in biological or synthetic scaffolds that mimic ECM to allow intercellular or matrix-cellular crosstalk. Because these structures are similar to organs in the body, they can be used as research models. Organoids are medicine's future hope for organ transplantation, tumor biobank formation, and the development of precision medicine. Organoid models can be used to study cell-to-cell interactions as well as effective factors like inflammation and aging. Bioengineering technologies are also used to define the size, shape, and composition of organoids before transforming them into precise structures. Finally, the importance of organoid applications in regenerative medicine has opened a new window for a better understanding of biological research, as discussed in this study.</p>","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"9 1","pages":"83-96"},"PeriodicalIF":2.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9122643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing Biomaterials for Immunomodulatory-Driven Tissue Engineering.","authors":"Justin X Zhong,&nbsp;Preethi Raghavan,&nbsp;Tejal A Desai","doi":"10.1007/s40883-022-00279-6","DOIUrl":"10.1007/s40883-022-00279-6","url":null,"abstract":"<p><strong>Abstract: </strong>The immune system plays a crucial role during tissue repair and wound healing processes. Biomaterials have been leveraged to assist in this in situ tissue regeneration process to dampen the foreign body response by evading or suppressing the immune system. An emerging paradigm within regenerative medicine is to use biomaterials to influence the immune system and create a pro-reparative microenvironment to instigate endogenously driven tissue repair. In this review, we discuss recent studies that focus on immunomodulation of innate and adaptive immune cells for tissue engineering applications through four biomaterial-based mechanisms of action: biophysical cues, chemical modifications, drug delivery, and sequestration. These materials enable augmented regeneration in various contexts, including vascularization, bone repair, wound healing, and autoimmune regulation. While further understanding of immune-material interactions is needed to design the next generation of immunomodulatory biomaterials, these materials have already demonstrated great promise for regenerative medicine.</p><p><strong>Lay summary: </strong>The immune system plays an important role in tissue repair. Many biomaterial strategies have been used to promote tissue repair, and recent work in this area has looked into the possibility of doing repair by tuning. Thus, we examined the literature for recent works showcasing the efficacy of these approaches in animal models of injuries. In these studies, we found that biomaterials successfully tuned the immune response and improved the repair of various tissues. This highlights the promise of immune-modulating material strategies to improve tissue repair.</p>","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"9 2","pages":"224-239"},"PeriodicalIF":2.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10272262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9798815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Bone and Dentin Matrix Derivatives on the Differentiation of Human Dental Pulp Stem Cells for Osteogenesis and Dentinogenesis in a Scaffold-Free Culture","authors":"M. Jalili Sadrabad, H. Sameni, Sam Zarbakhsh, R. Ghorbani, Amin Naghipoor, Alireza Jarahi","doi":"10.1007/s40883-022-00291-w","DOIUrl":"https://doi.org/10.1007/s40883-022-00291-w","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"5 1","pages":"416 - 423"},"PeriodicalIF":2.6,"publicationDate":"2022-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82080126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Different Routes of Xenogeneic Mesenchymal Stem Cell Transplantation on the Regenerative Potential of Spinal Cord Injury","authors":"A. Suvarna, M. Hoque, A. Saxena, Vineet Kumar, Rajendra Singh, S. Bag","doi":"10.1007/s40883-022-00290-x","DOIUrl":"https://doi.org/10.1007/s40883-022-00290-x","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"49 1","pages":"407 - 415"},"PeriodicalIF":2.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76642332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemocompatibility of all-trans retinoic acid-loaded citrate polymer coatings for vascular stents.","authors":"Heather Ursino, Bisheng Zhang, Christopher Ludtka, Antonio Webb, Josephine B Allen","doi":"10.1007/s40883-022-00257-y","DOIUrl":"10.1007/s40883-022-00257-y","url":null,"abstract":"<p><strong>Purpose: </strong>Current strategies implementing drug-eluting polymer stent coatings fail to fully address the lasting effects of endothelial suppression which ultimately result in delayed reendothelialization and thrombogenic complications. The present study investigates the <i>in vitro</i> hemocompatibility of all-trans retinoic acid loaded poly (1,8-octanediol-co-citrate) coatings (AtRA-POC coatings) for advanced intravascular stent technology. The ability of these materials in supporting endothelial restoration via migration and proliferation while inhibiting smooth muscle cell growth is also explored.</p><p><strong>Methods: </strong>Using in vitro models, the hemocompatibility of AtRA-loaded POC-coated cobalt chromium (CoCr) vascular stents was evaluated in terms of platelet and inflammatory activity. Platelet activity was quantified by platelet adhesion and platelet activation, further supported by SEM visualization. Inflammatory activity was quantified by the production of proinflammatory cytokines by THP1 monocytes. Lastly, <i>in vitro</i> wound healing and an 5-Ethynyl-2'deoxyuridine (EdU) and pico green DNA assays were used in quantitating endothelial and smooth muscle cell migration and proliferation.</p><p><strong>Results: </strong>Experimental examinations of platelet adhesion and activation demonstrate significant reductions in the platelet response to POC coated AtRA loaded stents when compared to bare CoCr stents. Such findings reveal AtRA-POC coatings to have significantly improved hemocompatibility compared to that of bare metal stents and at least as good as POC alone. Similarly, in reference to LPS-stimulated controls, Human monocyte-like THP1 cells in culture with AtRA-POC-CoCr stents for 24 hours showed reduced detection of proinflammatory cytokines, comparable to that of bare CoCr and untreated controls. This result supports AtRA-POC coatings as possessing limited immunological potential. Observations from <i>in vitro</i> endothelial and smooth muscle cell investigations demonstrate the ability of the drug AtRA to allow cell processes involved in restoration of the endothelium while inhibiting smooth muscle cell processes.</p><p><strong>Conclusion: </strong>This study demonstrates AtRA loaded POC coatings are hemocompatible, noninflammatory, and provide a promising strategy in enhancing vascular stent techniques and clinical integration. Possessing hemocompatibility and immunological compatibility that is at least as good as bare metal stents as clinical standards support the use of AtRA-POC coatings for vascular applications. Additionally, selectively reducing smooth muscle cell proliferation while supporting endothelial cell proliferation and migration further demonstrates the potential of these materials in significantly improving the state of vascular stent technology in the area of stent thrombosis and neointimal hyperplasia.</p>","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"8 4","pages":"579-592"},"PeriodicalIF":2.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10592068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"4D Printing in Biomedical Engineering: a State-of-the-Art Review of Technologies, Biomaterials, and Application","authors":"Souvik Ghosh, Siddhi Chaudhuri, Partha Roy, D. Lahiri","doi":"10.1007/s40883-022-00288-5","DOIUrl":"https://doi.org/10.1007/s40883-022-00288-5","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"8 1","pages":"339 - 365"},"PeriodicalIF":2.6,"publicationDate":"2022-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78881553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autologous Culture Expanded Iliac Crest Chondrocytes in Chitosan Hyaluronic Acid Dialdehyde Gel Regenerate Caprine Growth Plate","authors":"V. Madhuri, S. Ramesh, Karthikeyan Rajagopal, S. Chilbule, P. Nair, N. M. Walter","doi":"10.1007/s40883-022-00289-4","DOIUrl":"https://doi.org/10.1007/s40883-022-00289-4","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"4 1","pages":"397 - 406"},"PeriodicalIF":2.6,"publicationDate":"2022-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80941012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth Hormone Cartilage Regenerative Potential for Knee Osteoarthritis: a Systematic Review of Preclinical Animal Studies","authors":"A. Lubis, I. H. Dilogo, Natasena Galar Perwida, Safinah Aulia Sani, R. A. Rasyidah, Bernadus Riyan Hartanto","doi":"10.1007/s40883-022-00287-6","DOIUrl":"https://doi.org/10.1007/s40883-022-00287-6","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"3499 1","pages":"328 - 338"},"PeriodicalIF":2.6,"publicationDate":"2022-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86648143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Mechanisms Underlying the Short-Term Intervention of Forskolin-Mediated Bone Regeneration","authors":"Guleid M Awale, H. Kan, C. Laurencin, K. W. Lo","doi":"10.1007/s40883-022-00285-8","DOIUrl":"https://doi.org/10.1007/s40883-022-00285-8","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"9 1","pages":"375 - 383"},"PeriodicalIF":2.6,"publicationDate":"2022-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75648633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell-Based and Gene-Based Therapy Approaches in Neuro-orthopedic Disorders: a Literature Review","authors":"Amir Hossein Irajian, A. Presedo, Boshra Akbarzadeh Pasha, Mitra Ghasemi, M. Arabi, S. Abdi, Masoumeh Firouzi Sheshtamed, M. Nabian","doi":"10.1007/s40883-022-00284-9","DOIUrl":"https://doi.org/10.1007/s40883-022-00284-9","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"81 1","pages":"315 - 327"},"PeriodicalIF":2.6,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83142464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}