Regenerative Engineering and Translational Medicine最新文献

{"title":"Multiple Drug Delivery Ability of Calcium Phosphate Scaffolds promotes Osteogenic Gene Expression in In Vitro Co‑culture Model.","authors":"Naboneeta Sarkar, Yongdeok Jo, Priya Kushram, Susmita Bose","doi":"10.1007/s40883-025-00439-4","DOIUrl":"10.1007/s40883-025-00439-4","url":null,"abstract":"<p><strong>Purpose: </strong>This study investigates the incorporation of curcumin, resveratrol, and vitamin D3 into polymer-integrated calcium phosphate (CaP) scaffolds to assess their effects on osteogenic gene expression and osteosarcoma cell viability. Polymeric micelles and a polycaprolactone-polyethylene glycol (PCL-PEG) system were utilized to enhance drug loading, release, and bioactivity.</p><p><strong>Methods: </strong>The scaffolds were functionalized with curcumin, resveratrol, and vitamin D3 using different polymeric carriers for controlled release. The release profile of these drugs was evaluated at physiological and acidic pH conditions. Osteoblast proliferation and differentiation were assessed. An osteoblast and osteoclast co-culture and RT-qPCR were performed to investigate osteogenic and osteoclastic gene expression. Resorption pit formation and Tartrate-Resistant Acid Phosphatase (TRAP) assays were conducted to analyze osteoclast activity. Lastly, osteosarcoma cell viability and morphology were examined at multiple time points to determine the anti-osteosarcoma efficacy of the drugs in vitro.</p><p><strong>Results: </strong>The drug release study shows sustained release over a period of three days. At pH 5.0, the cumulative release of curcumin, resveratrol, and vitamin D3 reached 64%, 100%, and 80%, respectively. At pH 7.4, the corresponding release values were 25% for curcumin, 69% for vitamin D3, and 92% for resveratrol. In vitro, treated scaffolds enhance osteoblast proliferation by 1.1to 1.3-fold and upregulate key osteogenic markers. Osteoclast activity was significantly reduced, with smaller resorption pits and decreased TRAP activity. Osteosarcoma viability decreases by 2.5to 2.8-fold by day 11, indicating anti-cancer efficacy of the drugs.</p><p><strong>Conclusion: </strong>The scaffolds successfully delivered bioactive compounds that promote osteoblast growth while reducing osteoclast activity and osteosarcoma cell proliferation. This multifunctional approach demonstrates the potential for enhancing bone regeneration and minimizing tumor recurrence post-surgery.</p>","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"12 1","pages":"332-343"},"PeriodicalIF":3.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13186414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147982807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprintable Janus Base Nano-Matrix for Improved Cartilage Tissue Engineering.","authors":"Leah Faber, Anne Yau, Ryan Stack, Yupeng Chen","doi":"10.1007/s40883-025-00396-y","DOIUrl":"10.1007/s40883-025-00396-y","url":null,"abstract":"<p><strong>Purpose: </strong>Bioprinting is an additive manufacturing technique used to print living cells within a three-dimensional scaffold that mimics natural tissue microenvironments. There are several disadvantages to using hydrogel-based biomaterials for bioprinting including limited cell adhesion and functionality. To address this, we have developed a library of Janus base Nano-Matrices (JBNms) which are novel nanoscale scaffolds self-assembled from DNA-inspired Janus base nanotubes (JBNts) and ECM molecules. In this study, JBNms are incorporated in bioprinting by printing a cartilage-specific JBNm with human mesenchymal stem cells (hMSCs) into a 3D alginate scaffold to selectively improve chondro-lineage cell adhesion and differentiation.</p><p><strong>Methods: </strong>Human mesenchymal stem cells (hMSCs) were combined with a cartilage-specific JBNm and printed within an alginate-based bioink. They were maintained in chondrogenic media and were characterized at 7 and 28 days. Reverse transcription quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) and histological staining were used to determine the presence of cartilage-specific genes and proteins.</p><p><strong>Results: </strong>The bioprinted structures with the cartilage-specific JBNm showed significantly greater expression of chondrogenic-related marker genes and glycosaminoglycan (GAG) expression after 28 days compared to the negative control group, indicating successful chondrogenesis. The cells were viable within the structures and showed significant proliferation after 28 days.</p><p><strong>Conclusion: </strong>Cartilage-specific JBNm scaffolds successfully promote enhanced hMSC adhesion, growth, and chondrogenic differentiation within bioprinted constructs.</p>","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"12 1","pages":"189-201"},"PeriodicalIF":3.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13384489/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148606738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supraspinatus Muscle Degeneration, Inflammation, and Regeneration Vary By Location in a Rat Model of Severe Rotator Cuff Tear.","authors":"Molly E Ogle, Kasheena Box, Keshav R Shah, Johnna S Temenoff","doi":"10.1007/s40883-024-00343-3","DOIUrl":"10.1007/s40883-024-00343-3","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Purpose: &lt;/strong&gt;Degeneration of the human supraspinatus muscle after rotator cuff tendon tear varies by location within the muscle; however, the localization of changes to muscle fiber size, amount of regenerating fibers, and cellular immune response have not been previously characterized. Due to the proximity to the injured tendon, we hypothesized that these processes are more pronounced near the lateral myotendinous junction region of the muscle and the intramuscular tendon.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Spatially-defined immunohistochemical analysis was used to evaluate lengthwise and radial differences within the supraspinatus muscle in a rat model of full supraspinatus and infraspinatus tendon transection and subscapular denervation. Muscle fiber diameter was quantified, regeneration was evaluated by myofiber central nuclei and embryonic myosin heavy chain, and the immune environment was assessed by the presence of macrophage subsets and T cells.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Degeneration (smaller fiber diameter), regeneration (embryonic myosin heavy chain-positive fibers), and macrophage infiltration are more substantial in the lateral muscle near the myotendinous junction and radially near the intramuscular tendon. The muscle adjacent to the myotendinous junction had a balanced M1-like and M2-like macrophage response up to 2 weeks post-injury followed by an M1-dominant inflammation. The medial muscle belly maintained an M1-dominant response throughout 3 weeks. T cells were not highly abundant, however, were most elevated one week after injury near the intramuscular tendon. Fibrous infiltration was observed 1-week post-injury throughout the muscle, while fatty infiltration was elevated after 3 weeks only in the lateral muscle region.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;Together, these findings suggest that the supraspinatus muscle undergoes regional changes (particularly muscle fiber size, markers of regeneration, and macrophage infiltration) after supraspinatus tendon injury.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Lay summary and future work: &lt;/strong&gt;Rotator cuff tendon tear in the rat shoulder leads to a greater inflammatory response and a greater early pro-regenerative response in the supraspinatus muscle spatially near the muscle-tendon attachment compared with areas deeper in the muscle in the first two weeks after the tendon is injured. Both fatty and fibrous muscle degeneration were observed over three weeks throughout the muscle, with less spatial localization. Together, these data indicate that there are regional differences in damage and repair of the supraspinatus muscle early post-injury. These findings may help interpret results from clinical studies of muscle degeneration after rotator cuff tear and may lead to improved, spatially-targeted approaches for delivery of therapeutics to muscle after rotator cuff tendon tear.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Supplementary information: &lt;/strong&gt;The online version contains supplementary material available at 1","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"12 2","pages":"787-799"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13263273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating Green Nanomaterials and RNA Interference: A Combinatorial Approach for Breast Cancer Treatment","authors":"Muhammad Irfan, Hadeeqa Sagheer, Mahreen Elahi","doi":"10.1007/s40883-025-00529-3","DOIUrl":"https://doi.org/10.1007/s40883-025-00529-3","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147913890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epitope Imprinted Nanoparticles as Customized Synthetic Antibodies for Biorecognition in Theranostics.","authors":"Simão P B Teixeira, Rui L Reis, Rui M A Domingues","doi":"10.1007/s40883-025-00478-x","DOIUrl":"10.1007/s40883-025-00478-x","url":null,"abstract":"<p><p>As medicine advances towards an era of personalized and targeted therapies, the need for precise and reliable molecular recognition elements becomes increasingly critical. These elements are essential for directing therapeutic agents to specific cells or tissues, thereby maximizing efficacy while minimizing off-target effects. Among the emerging technologies, epitope-imprinted nanoparticles (EINPs) have demonstrated exceptional potential as \"synthetic antibodies\" for the recognition of biomarkers. These nanoparticles are designed to mimic the binding capabilities of natural antibodies but with enhanced stability, specificity, and ease of production. Unlike traditional antibodies, which can be costly, labile, and prone to significant side effects, EINPs offer a cost-effective, robust, and customizable platform for targeted sensing and therapeutic applications. This Perspective Review explores the design, synthesis, and application of epitope-imprinted nanoparticles, highlighting their advantages and potential to revolutionize the landscape of abiotic molecular recognition in disease detection and targeted therapies. Finally, we present perspectives on foreseeable breakthroughs and advances, offering insights into how molecular imprinting techniques can be expanded to new biomedical fields, such as tissue engineering.</p>","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7619072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147869199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D Printing of Enzymatically Softening Hydrogel Biomaterials.","authors":"Olivia P Dotson, Sherina Malkani, Inkyung Kang, Cole A DeForest, Kelly R Stevens","doi":"10.1007/s40883-025-00445-6","DOIUrl":"10.1007/s40883-025-00445-6","url":null,"abstract":"<p><strong>Purpose: </strong>3D printing has accelerated tissue engineering by enabling rapid fabrication of bioprinted tissues from a variety of soft biomaterials. Yet, an ongoing challenge is that for many bioprinting technologies, the materials (bioinks) need to be printed \"stiff\" (i.e., G' > ~ 15 kPa) so that the fabricated tissue constructs retain high resolution and shape fidelity. Conversely, softer materials tend to generally be more supportive of cellular phenotype and function. To bridge this gap, we sought to develop a hydrogel system that would expand bioprinting access to softer materials, while retaining the resolution of fabricated spatial features.</p><p><strong>Methods: </strong>We developed a photopolymerizable copolymer hydrogel system consisting of nondegradable synthetic and proteolytically degradable natural polymers. Varying the overall polymer content, as well as the ratio between the poly(ethylene glycol) and gelatin species, we generated a library of lithographically printable hydrogel formulations with differing initial stiffnesses that could be further variably softened following enzymatic treatment using collagenase.</p><p><strong>Results: </strong>Varying the copolymer composition and overall concentration resulted in the creation of gels whose initial stiffness ranged from 82 to 2 kPa and could be subsequently softened up to 20-fold upon enzymatic treatment. When 3D-printed via digital light processing (DLP), softened gels maintained higher structural integrity than those with matched initial stiffness. Softened gels supported greater endothelial cell perfusion-based seeding compared to those untreated while maintaining high cell viability.</p><p><strong>Conclusion: </strong>Our material system presents a simple solution to the ongoing challenge of 3D-printing soft materials with high resolution.</p><p><strong>Future work: </strong>In future studies, we will develop post-print softening materials with bio-invisible stimuli to expand applications to in vivo softening of biomaterial tissue mimics.</p><p><strong>Lay summary: </strong>3D-printing has become popular in tissue engineering applications, but printing complex, organ-like structures with soft materials remains challenging. We created a material that can hold patterned shapes and small printed structures using a post-print softening technique with a degrading enzyme. We found that different formulations of this hydrogel material offer varying stiffness levels (G' = 2 kPa-82 kPa) and can soften up to 20-fold with enzymatic treatment. Notably, this material retains the structure of 3D-printed open channels even after significant softening, and cells respond well when seeded in these channels. This demonstrates the promise of post-print softening to create soft 3D-printed materials.</p>","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"11 4","pages":"1099-1108"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13221206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148138863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic Methods for Assembling and Modifying Hydrogel Biomaterials.","authors":"Irina Kopyeva, Cole A DeForest","doi":"10.1007/s40883-025-00426-9","DOIUrl":"10.1007/s40883-025-00426-9","url":null,"abstract":"<p><strong>Purpose: </strong>Enzymatic reactions offer many advantages for hydrogel synthesis and modification, due to their gentle reaction conditions, biocompatibility, and diversity of substrates.</p><p><strong>Methods: </strong>In this review, we examine the current body of literature through databases such as Google Scholar, PubMed, and Web of Science.</p><p><strong>Results: </strong>Various enzyme classes have been utilized for hydrogel assembly and disassembly, including transglutaminases, oxidoreductases, transpeptidases, and proteinases. The enzymatic substrates can be readily included in peptide precursors and/or appended onto synthetic polymers. We discuss the benefits and limitations of each system, with a focus on ease of use/synthesis, accessibility, and financial considerations.</p><p><strong>Conclusion: </strong>Enzymes are frequently utilized to modify both natural and synthetic biomaterials. For developing more advanced, stimuli-responsive platforms, \"biologically invisible\" enzymes such as sortases should be leveraged to not interfere with native processes and/or the mammalian proteome.</p><p><strong>Lay summary: </strong>Enzymes, proteins that act as biological catalysts, are an important tool for making and breaking down hydrogels, or water-swollen polymeric networks, for various biomedical applications. In particular, these techniques have seen great usage for modeling the tissue environment for lab-based assays.</p>","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"11 4","pages":"893-904"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13186249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147982794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Pressure Regulator Platform for Applying Biomechanical Stimuli on Organ-on-A-Chip Systems with Physiological and Pathological Relevancy.","authors":"Carlos Ezio Garciamendez-Mijares, Francisco Aguilar Rojas, David S Rendon Ruiz, Xuan Mei, Pavel Hernandez, Begoña Sanchez Gonzalez, Jose Gerardo Marin Canchola, Victoria Abril Manjarrez Rivera, Ricardo Rodriguez, Francisco Lugo Mestre, Sushila Maharjan, Shayan Gholizadeh, Marie Denis Gerhard-Herman, Yu Shrike Zhang","doi":"10.1007/s40883-025-00448-3","DOIUrl":"10.1007/s40883-025-00448-3","url":null,"abstract":"<p><strong>Purpose: </strong>The organ-on-a-chip (OOC) technology has transformed <i>in vitro</i> modeling by replicating human organ microenvironments with high fidelity, offering improved platforms for drug discovery and disease modeling. However, existing biomechanical stretch-compression platforms are often costly, rely on proprietary chip designs, and lack flexibility in generating (patho)physiological waveforms. These limitations hinder the accurate replication of dynamic biomechanical cues experienced by tissues and organs <i>in vivo</i>. This study presents the Pressure Regulator Platform (PRP), a low-cost, chip-agnostic system designed to deliver customizable and patient-specific stretch-compression biomechanical stimuli to OOC devices.</p><p><strong>Methods: </strong>The PRP integrates hardware, electronics, and software to enable real-time generation of user-defined mechanical waveforms. Users can input patient-derived waveform profiles or select predefined waveforms, modifying frequency and amplitude to match physiological and pathological conditions. The PRP was tested on a blood vessel-on-a-chip model, evaluating its ability to replicate vascular biomechanics by applying controlled strain through vacuum-induced membrane deformation.</p><p><strong>Results: </strong>The PRP successfully reproduced patient-derived waveform profiles with high accuracy. The chip-agnostic design approach allowed seamless integration with multiple OOC configurations. Furthermore, this platform-maintained error levels below 1% for stabilized generic waveforms and achieved controlled vascular biomechanics in the OOC model, facilitating unidirectional alignment of vascular smooth muscle cells.</p><p><strong>Conclusion: </strong>The PRP provides a flexible and accessible platform for customizable and patient-derived biomechanical stimulation, enhancing the physiological relevance of <i>in vitro</i> models. Its capability to replicate patient-specific biomechanical conditions paves the way for applications in drug discovery, disease modeling, and personalized medicine.</p>","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"11 4","pages":"1083-1098"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12962595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147378317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silk Fibroin Particle-Laden Sponges as a Multiphase Controlled Release Platform.","authors":"Marisa O Pacheco, Cathrine A Beshay, Whitney L Stoppel","doi":"10.1007/s40883-025-00427-8","DOIUrl":"10.1007/s40883-025-00427-8","url":null,"abstract":"<p><strong>Purpose: </strong>Silk fibroin-based biomaterials have shown utility across regenerative medicine applications due to their ability to provide robust mechanical support and deliver bioactive cargo. To achieve diverse functions, fibroin can be fabricated into material formats with varied morphology including sponge-like scaffolds and microparticles. This study investigates the potential of a dual-component silk fibroin system (particle-laden sponges) to enable two-phase controlled release and assesses the impact of cytokine release on RAW 264.7 polarization.</p><p><strong>Methods: </strong>Silk fibroin microparticles (SFMPs) were prepared through phase separation from PVA before being combined with aqueous fibroin polymer solution at desired mass ratios. This solution was frozen and lyophilized to form a particle-laden sponge. The sponge was then water annealed to induce crystallinity at a set temperature. Sponge morphology was assessed with SEM and crystallinity was assessed with FTIR. <i>In vitro</i> accelerated degradation studies were used to identify candidate formulations for functional release experiments. For functional release analysis, M1 and M2 promoting cytokines were loaded into the sponge and the particle portions, respectively. Cytokine release was assessed using ELISA and using RT-qPCR, the polarization state of RAW 264.7 cells was captured following 1 and 3 days of incubation with the material.</p><p><strong>Results: </strong>Both formulation and temperature during water annealing were found to impact morphology and total crystalline content. Degradation studies showed disruption of the sponge like structures prior to SFMP degradation, indicating a potential for a multiphase controlled release platform. RAW 264.7 cells showed a polarization switch from M1 to M2 in line with hypothesized rates of release from the particle-laden sponge.</p><p><strong>Conclusion: </strong>This study demonstrated the tunability of a dual-component silk fibroin sponge, while also establishing its potential as a multiphase controlled release platform to modulate immune interactions in future <i>in vivo</i> studies.</p>","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"11 4","pages":"1067-1082"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12872209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146126260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal Modeling in Regenerative Medicine: Applications and Challenges in Tissue Engineering","authors":"Ravikumar Jayabal","doi":"10.1007/s40883-025-00484-z","DOIUrl":"https://doi.org/10.1007/s40883-025-00484-z","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147891119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}