Regenerative Engineering and Translational Medicine最新文献

{"title":"Delivery of Nucleic Acids for Vascular Tissue Engineering Applications.","authors":"Jordyn M Wyse, Priscilla Lopez, Dimitrios Miserlis, Panagiotis Koutakis, Marissa E Wechsler","doi":"10.1007/s40883-025-00421-0","DOIUrl":"10.1007/s40883-025-00421-0","url":null,"abstract":"<p><p>The biomedical research of nucleic acids as therapeutics and their medical applications has been steadily progressing. Identifying the fundamental regulatory roles of nucleic acids and their potential use in the medical field is of major pursuit in current and future research endeavors. Research into nucleic acids for the treatment of vascular diseases has been an emerging avenue as nucleic acids have the ability to treat a variety of pathologies including atherosclerosis. A large part of the translational research in relation to nucleic acids is the development and optimization of drug delivery technologies that can harness the full potential of these molecules, providing untapped, novel therapeutic agents. Specifically, the use of biomaterial delivery systems, consisting of polymers, lipids, and inorganic materials, allows for the protection of nucleic acid therapeutics to promote targeting to regions of vascular damage. While the relevance of nucleic acid therapeutics has been well documented, their functionality for diseases affecting the peripheral vasculature, and the need for biomaterials systems capable of improving their efficacy, has been lacking. This review aims to provide an overview of the biomaterial technologies tested for nucleic acid delivery, relative to the science of applied vascular tissue engineering.</p>","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward Bioactive Hydrogels: A Tunable Approach via Nucleic Acid-Collagen Complexation.","authors":"Nikolaos Pipis, Senthilkumar Duraivel, Vignesh Subramaniam, Kevin A Stewart, Thomas E Angelini, Josephine B Allen","doi":"10.1007/s40883-024-00345-1","DOIUrl":"10.1007/s40883-024-00345-1","url":null,"abstract":"<p><strong>Purpose: </strong>Nucleic acid-collagen complexes (NACCs) are unique biomaterials formed by binding short, monodisperse single-stranded DNA (ssDNA) with type I collagen. These complexes spontaneously generate microfibers and nanoparticles of varying sizes, offering a versatile platform with potential applications in tissue engineering and regenerative medicine. However, the detailed mechanisms behind the nucleic acid-driven assembly of collagen fibers still need to be established. We aim to understand the relationship between microscopic structure and bulk material properties and demonstrate that NACCs can be engineered as mechanically tunable systems.</p><p><strong>Methods: </strong>We present a study to test NACCs with varying molar ratios of collagen to random ssDNA oligonucleotides. Our methods encompass the assessment of molecular interactions through infrared spectroscopy and the characterization of gelation and rheological behavior. We also include phase contrast, confocal reflectance, and transmission electron microscopy to provide complementary information on the 3D structural organization of the hydrogels.</p><p><strong>Results: </strong>We report that adding DNA oligonucleotides within collagen robustly reinforces and rearranges the hydrogel network and accelerates gelation by triggering rapid fiber formation and spontaneous self-assembly. The elasticity of NACC hydrogels can be tailored according to the collagen-to-DNA molar ratio, ssDNA length, and collagen species.</p><p><strong>Conclusion: </strong>Our findings hold significant implications for the design of mechanically tunable DNA-based hydrogel systems. The ability to manipulate hydrogel stiffness by tailoring DNA content and collagen concentration offers new avenues for fine tuning material properties, enhancing the versatility of bioactive hydrogels in diverse biomedical applications.</p><p><strong>Lay summary: </strong>This work is an example of forming fibers and gels with tunable elasticity that stems from the complexation of short-length nucleic acids (on the order of size of aptamers) and collagen, which can be potentially extended to a variety of functionalized hydrogel designs and tailored biomedical applications. Incorporating DNA induces mechanical changes in NACCs.</p>","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"10 4","pages":"564-576"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12291172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shilajit Boosts Osteogenic Ability of Mesenchymal Stem Cells for Regeneration of Rat Bone Defect","authors":"Parisa Kangari, Leila Roshangar, Nader Tanideh, Farideh Afshari, N. Chenari, Tahereh Talaei-Khozani, M. Razmkhah","doi":"10.1007/s40883-023-00329-7","DOIUrl":"https://doi.org/10.1007/s40883-023-00329-7","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"11 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139445677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan in Wound Healing: a Mini Review on Ethical Perspective on Sustainable and Biomedical Biomaterials","authors":"Yerraboina Praneeth, Komal, Inderpal Devgon, Rohan Samir Kumar Sachan, Abhishek Rana, Arun Karnwal, Abhinav Kumar","doi":"10.1007/s40883-023-00330-0","DOIUrl":"https://doi.org/10.1007/s40883-023-00330-0","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"89 7","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138954474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decellularized Extracellular Matrix-Derived Hydrogels: a Powerful Class of Biomaterials for Skeletal Muscle Regenerative Engineering Applications","authors":"Mohammed A. Barajaa, Debolina Ghosh, C. Laurencin","doi":"10.1007/s40883-023-00328-8","DOIUrl":"https://doi.org/10.1007/s40883-023-00328-8","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"9 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138589741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogels Loaded with Mesenchymal Stem Cells Extracellular Vesicles for Treating Knee Joint Disorders: A Systematic Review","authors":"Homero Garcia-Motta, Mirian Bonifacio, C. Martignago, Lais Caroline Souza-Silva, Beatriz Soares-Silva, J. Parisi, Lívia Assis, Daniel Araki Ribeiro, Alessandra Mussi Ribeiro, A. Renno","doi":"10.1007/s40883-023-00326-w","DOIUrl":"https://doi.org/10.1007/s40883-023-00326-w","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"15 4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138601300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradable Polyphosphazenes for Biomedical Applications","authors":"Manaswee Barua, Oyindamola R. Teniola, C. Laurencin","doi":"10.1007/s40883-023-00318-w","DOIUrl":"https://doi.org/10.1007/s40883-023-00318-w","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":" 14","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138618510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Platelet-Rich Plasma and Adipose-Derived Stromal Vascular Fraction on Atrophic Non-union Fracture Healing in a Rabbit Model","authors":"K. Sharun, Abhijit M. Pawde, S. A. Banu, K. Manjusha, E. Kalaiselvan, Rohit Kumar, P. Kinjavdekar, Karam Pal Singh, M. Verma, Vikash Chandra, Reena Mukherjee, Amarpal","doi":"10.1007/s40883-023-00325-x","DOIUrl":"https://doi.org/10.1007/s40883-023-00325-x","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"44 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139211727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regenerative Engineering of a Limb: From Amputation to Regeneration","authors":"Amir Abbas Abedini, Fatemeh Hosseini, C. Laurencin","doi":"10.1007/s40883-023-00323-z","DOIUrl":"https://doi.org/10.1007/s40883-023-00323-z","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"28 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139234481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fat Expansion Not Fat Infiltration of Muscle Post Rotator Cuff Tendon Tears of the Shoulder: Regenerative Engineering Implications","authors":"Marc A. Merriman Jr, James H. Chapman, Taraje Whitfield, Fatemeh Hosseini, Debolina Ghosh, C. Laurencin","doi":"10.1007/s40883-023-00324-y","DOIUrl":"https://doi.org/10.1007/s40883-023-00324-y","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":"49 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139230807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}