Toward Bioactive Hydrogels: A Tunable Approach via Nucleic Acid-Collagen Complexation.

IF 1.9 Q3 ENGINEERING, BIOMEDICAL
Nikolaos Pipis, Senthilkumar Duraivel, Vignesh Subramaniam, Kevin A Stewart, Thomas E Angelini, Josephine B Allen
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引用次数: 0

Abstract

Purpose: Nucleic acid-collagen complexes (NACCs) are unique biomaterials formed by binding short, monodisperse single-stranded DNA (ssDNA) with type I collagen. These complexes spontaneously generate microfibers and nanoparticles of varying sizes, offering a versatile platform with potential applications in tissue engineering and regenerative medicine. However, the detailed mechanisms behind the nucleic acid-driven assembly of collagen fibers still need to be established. We aim to understand the relationship between microscopic structure and bulk material properties and demonstrate that NACCs can be engineered as mechanically tunable systems.

Methods: We present a study to test NACCs with varying molar ratios of collagen to random ssDNA oligonucleotides. Our methods encompass the assessment of molecular interactions through infrared spectroscopy and the characterization of gelation and rheological behavior. We also include phase contrast, confocal reflectance, and transmission electron microscopy to provide complementary information on the 3D structural organization of the hydrogels.

Results: We report that adding DNA oligonucleotides within collagen robustly reinforces and rearranges the hydrogel network and accelerates gelation by triggering rapid fiber formation and spontaneous self-assembly. The elasticity of NACC hydrogels can be tailored according to the collagen-to-DNA molar ratio, ssDNA length, and collagen species.

Conclusion: Our findings hold significant implications for the design of mechanically tunable DNA-based hydrogel systems. The ability to manipulate hydrogel stiffness by tailoring DNA content and collagen concentration offers new avenues for fine tuning material properties, enhancing the versatility of bioactive hydrogels in diverse biomedical applications.

Lay summary: This work is an example of forming fibers and gels with tunable elasticity that stems from the complexation of short-length nucleic acids (on the order of size of aptamers) and collagen, which can be potentially extended to a variety of functionalized hydrogel designs and tailored biomedical applications. Incorporating DNA induces mechanical changes in NACCs.

生物活性水凝胶:通过核酸-胶原络合的可调方法。
目的:核酸-胶原复合物(NACCs)是由短的、单分散的单链DNA (ssDNA)与I型胶原结合而形成的独特生物材料。这些复合物可以自发地产生不同大小的微纤维和纳米颗粒,为组织工程和再生医学提供了一个具有潜在应用前景的多功能平台。然而,核酸驱动胶原纤维组装背后的详细机制仍然需要建立。我们的目标是了解微观结构和块状材料特性之间的关系,并证明NACCs可以被设计为机械可调系统。方法:我们提出了一项研究,以不同的胶原与随机ssDNA寡核苷酸的摩尔比来测试NACCs。我们的方法包括通过红外光谱评估分子相互作用和表征凝胶和流变行为。我们还包括相衬、共聚焦反射和透射电子显微镜,以提供有关水凝胶三维结构组织的补充信息。结果:我们报道,在胶原蛋白中添加DNA寡核苷酸可以增强和重新排列水凝胶网络,并通过触发快速纤维形成和自发自组装来加速凝胶化。NACC水凝胶的弹性可以根据胶原与dna的摩尔比、ssDNA的长度和胶原的种类来定制。结论:我们的发现对机械可调dna水凝胶系统的设计具有重要意义。通过调整DNA含量和胶原蛋白浓度来操纵水凝胶硬度的能力为微调材料特性提供了新的途径,增强了生物活性水凝胶在各种生物医学应用中的多功能性。总结:这项工作是形成具有可调弹性的纤维和凝胶的一个例子,源于短长度核酸(适体大小的顺序)和胶原的络合,这可以潜在地扩展到各种功能化水凝胶设计和定制的生物医学应用。加入DNA可诱导NACCs发生机械变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.90
自引率
11.50%
发文量
41
期刊介绍: Regenerative Engineering is an international journal covering convergence of the disciplines of tissue engineering, advanced materials science, stem cell research, the physical sciences, and areas of developmental biology. This convergence brings exciting opportunities to translate bench-top research into bedside methods, allowing the possibility of moving beyond maintaining or repairing tissues to regenerating them. The journal encourages both top-down engineering approaches and bottom-up strategies integrating materials science with stem cell research and developmental biology. Convergence papers on instructive biomaterials, stimuli-responsive biomaterials, micro- and nano-patterning for regenerative engineering, elastomeric biomaterials, hydrogels for tissue engineering, and rapid prototyping and bioprinting approaches are particularly welcome. The journal provides a premier, single-blind peer-reviewed forum for the publication of original papers, authoritative reviews, rapid communications, news and views, and opinion papers addressing the most important issues and efforts toward successfully regenerating complex human tissues and organs. All research articles feature a lay abstract highlighting the relevance and future impact for patients, government and other health officials, and members of the general public. Bridging the gap between the lab and the clinic, the journal also serves as a dedicated platform for showcasing translational research that brings basic scientific research and discoveries into clinical methods and therapies, contributing to the improvement of human health care. Topics covered in Regenerative Engineering and Translational Medicine include: Advanced materials science for regenerative and biomedical applicationsStem cells for tissue regenerationDrug delivery for tissue regenerationNanomaterials and nanobiotechnology for tissue regenerationStudies combining tissue engineering/regeneration with developmental biologyConvergence research in pre-clinical and clinical phases
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