Prostaglandins最新文献_第2页

Effects of Organic Anions and Bile Acid Conjugates on Biliary Excretion of LTC4 in the Rat 有机阴离子和胆汁酸偶联物对大鼠胆道LTC4排泄的影响

Prostaglandins Pub Date : 1997-11-01 DOI: 10.1016/S0090-6980(97)00163-9

Ken-ichi Kitaura, Hajime Takikawa, Masami Yamanaka

{"title":"Effects of Organic Anions and Bile Acid Conjugates on Biliary Excretion of LTC4 in the Rat","authors":"Ken-ichi Kitaura, Hajime Takikawa, Masami Yamanaka","doi":"10.1016/S0090-6980(97)00163-9","DOIUrl":"10.1016/S0090-6980(97)00163-9","url":null,"abstract":"<div>Biliary organic anion excretion is mediated by an ATP-dependent primary active transporter, so-called canalicular multispecific organic anion transporter (cMOAT). On the other hand, a multiplicity of canalicular organic anion transport has been suggested. Therefore, to examine the substrate specificity of cMOAT using inhibition of excretion of [3H] LTC4-derived radioactive products in the bile as a marker, we examined the effects of various organic anions and bile acid conjugates on the biliary excretion of LTC4 in rats. Biliary excretion of the metabolites of [3H] LTC4, which was injected via the femoral vein, was markedly inhibited by sulfobromophthalein-glutathione, taurolithocholate-3-sulfate, and ursodeoxycholate-3-O-glucuronide. In contrast, dibromosulfophthalein and cefpiramide slightly inhibited, and pravastatin, taurocholate, and 3,7-sul-UDC did not affect biliary LTC4 excretion. Furthermore, vinblastine and phenothiazine, a P-glycoprotein substrate and inducer, did not affect biliary LTC4 excretion. Among various organic anions and bile acid conjugates, LTC4, sulfobromophthalein-glutathione, taurolithocholate-3-sulfate, and ursodeoxycholate-3-O-glucuronide may be good substrates for cMOAT.</div>","PeriodicalId":20653,"journal":{"name":"Prostaglandins","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0090-6980(97)00163-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20415404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Effects of Nonsteroidal Anti-Inflammatory Drugs on Interleukin-1 Receptor Antagonist Production in Cultured Human Peripheral Blood Mononuclear Cells 非甾体抗炎药对培养的人外周血单个核细胞白细胞介素-1受体拮抗剂产生的影响

Prostaglandins Pub Date : 1997-11-01 DOI: 10.1016/S0090-6980(97)00159-7

Hidenobu Kusuhara, Hirofumi Matsuyuki, Takeki Okumoto

{"title":"Effects of Nonsteroidal Anti-Inflammatory Drugs on Interleukin-1 Receptor Antagonist Production in Cultured Human Peripheral Blood Mononuclear Cells","authors":"Hidenobu Kusuhara, Hirofumi Matsuyuki, Takeki Okumoto","doi":"10.1016/S0090-6980(97)00159-7","DOIUrl":"10.1016/S0090-6980(97)00159-7","url":null,"abstract":"<div>The effects of nonsteroidal anti-inflammatory drugs (NSAIDs), mofezolac, indomethacin, sodium diclofenac, and zaltoprofen, on the production of interleukin-1 receptor antagonist (IL-1ra) were examined in cultured human peripheral blood mononuclear cells (PBMC). Among the NSAIDs tested, mofezolac and sodium diclofenac were found to stimulate the mRNA expression for IL-1ra without affecting the mRNA expression for IL-1β. These two drugs also stimulated the secretion of IL-1ra by PBMC in the absence of bacterial lipopolysaccharide (LPS), however, the stimulatory effect of sodium diclofenac diminished in the presence of LPS. Mofezolac suppressed the mRNA expression for IL-1β in PBMC stimulated with exogenous IL-1β, indicating the secreted IL-1ra in the presence of mofezolac to be biologically active. Since IL-1ra suppresses the function of IL-1, a pro-inflammatory cytokine, the stimulatory effect of such NSAIDs as mofezolac on IL-1ra production could also be one of the mechanisms involved in its anti-inflammatory and antinociceptive actions.</div>","PeriodicalId":20653,"journal":{"name":"Prostaglandins","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0090-6980(97)00159-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20415871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

In Vitro Effects of Prostaglandin E2 or Indomethacin on the Proliferation of Lymphokine-Activated Killer Cells and their Cytotoxicity against Bladder Tumor Cells in Patients with Bladder Cancer 前列腺素E2或吲哚美辛对膀胱癌患者淋巴因子激活杀伤细胞增殖的影响及其对膀胱肿瘤细胞的细胞毒性

Prostaglandins Pub Date : 1997-11-01 DOI: 10.1016/S0090-6980(97)00161-5

Wang Zhiping , Chen Yirong , Zheng Rongliang , Qin Dashan , Chen Xuehong , Wang Yiqiu , Liu Guodong

{"title":"In Vitro Effects of Prostaglandin E2 or Indomethacin on the Proliferation of Lymphokine-Activated Killer Cells and their Cytotoxicity against Bladder Tumor Cells in Patients with Bladder Cancer","authors":"Wang Zhiping , Chen Yirong , Zheng Rongliang , Qin Dashan , Chen Xuehong , Wang Yiqiu , Liu Guodong","doi":"10.1016/S0090-6980(97)00161-5","DOIUrl":"10.1016/S0090-6980(97)00161-5","url":null,"abstract":"<div>Purpose: To investigate the combined effects of interleukin-2 (IL-2) with either prostaglandin E2 (PGE2) or indomethacin (IM) on the proliferation and cytolysis of bladder tumor cells by lymphokine-activated killer (LAK) cells in patients with bladder cancer.Methods: LAK cell proliferation was assayed in the presence of various concentrations of either PGE2 or IM by cell counting. Bladder cancer cell lines BIU-87, EJ and bladder tumor cells (BTC) from the patients were cultured as target cells, and the cytotoxicity of LAK cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In addition, PGE2 in samples of conditioned medium from bladder cancer cells or peripheral blood mononuclear cells (PBMC) as well as plasma from 21 patients with bladder cancer and 20 healthy donors were determined by radioimmunoassay (RIA).Results: The proliferation of LAK cells induced by IL-2 was inhibited by PGE2 (0.05 to 5 ng/mL) in concentration-dependent manner. The enhanced growth of LAK cells was observed at certain concentrations of IM (100–400 ng/mL) from 48 to 96 h. Pretreatment of LAK cells with IM (200 ng/mL) significantly enhanced cytotoxicity against BIU-87, EJ cells, or BTC. More PGE2 was present in conditioned medium from BIU-87 cells than in the conditioned medium from PBMC.Conclusions: These studies indicate that LAK cell proliferation induced by IL-2 in patients with bladder cancer is inhibited by PGE2 produced by PBMC and bladder cancer cells. This inhibition can be overcome by IM, which may be of use in immunotherapy of bladder cancer.</div>","PeriodicalId":20653,"journal":{"name":"Prostaglandins","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0090-6980(97)00161-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20415407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Cytochrome P450 Metabolites of Arachidonic Acid in Human Placenta 花生四烯酸在人胎盘中的细胞色素P450代谢物

Prostaglandins Pub Date : 1997-10-01 DOI: 10.1016/S0090-6980(97)00148-2

W.R Schaefer , K Werner , H Schweer , J Schneider , E Arbogast , H.P Zahradnik

{"title":"Cytochrome P450 Metabolites of Arachidonic Acid in Human Placenta","authors":"W.R Schaefer , K Werner , H Schweer , J Schneider , E Arbogast , H.P Zahradnik","doi":"10.1016/S0090-6980(97)00148-2","DOIUrl":"10.1016/S0090-6980(97)00148-2","url":null,"abstract":"<div>Little is known about the epoxygenase pathway of the arachidonic acid cascade in uterine tissues. In this paper, we describe the formation of epoxyeicosatrienoic acids (EETs) and dihydroxyeicosatrienoic acids (DHETs) in human term placenta after uncomplicated pregnancies. Metabolism of [3H]-arachidonic acid was analyzed in short term tissue cultures of placenta by reverse phase HPLC. Major metabolites coeluted with authentic EETs and DHETs. The formation of EETs was inhibited by carbon monoxide. In non-radioactive incubations with biopsies from seven different placentas, sufficient material for GC/MS analysis was obtained. The combined media were purified by solid phase extraction and reverse phase HPLC. The fraction coeluting with DHETs was derivatized with pentafluorobenzylbromide (PFB) and bis-(trimethylsilyl)-trifluoroacetylacetamide (BSTFA) and analyzed by GC/NICI/MS/MS. 11,12-DHET and 14,15-DHET were identified by their mass spectra displaying specific fragments at m/z 149 and m/z 189, respectively. Our results suggest that the epoxygenase pathway is active in human term placenta.</div>","PeriodicalId":20653,"journal":{"name":"Prostaglandins","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0090-6980(97)00148-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20367305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Molecular Cloning and Expression of a Rat Prostaglandin E2 Receptor of the EP2 Subtype 1 2 EP2亚型1大鼠前列腺素E2受体的克隆与表达

Prostaglandins Pub Date : 1997-10-01 DOI: 10.1016/S0090-6980(97)00145-7

Ken Nemoto , Carol C Pilbeam , Stephan R Bilak , Lawrence G Raisz

{"title":"Molecular Cloning and Expression of a Rat Prostaglandin E2 Receptor of the EP2 Subtype 1 2","authors":"Ken Nemoto , Carol C Pilbeam , Stephan R Bilak , Lawrence G Raisz","doi":"10.1016/S0090-6980(97)00145-7","DOIUrl":"https://doi.org/10.1016/S0090-6980(97)00145-7","url":null,"abstract":"<div>Prostaglandin E2 (PGE2) is a potent local mediator of cell growth and differentiation in various tissues. The receptors for PGE2 have been classified into four pharmacological subtypes, EP1, EP2, EP3, and EP4, based on the responses to selective agonists and antagonists. We have cloned a functional cDNA for the rat EP2 receptor subtype from a rat lung cDNA library. The rat EP2 receptor cDNA encodes 357 amino acid residues having high homology with the human and mouse EP2 receptors and containing seven putative transmembrane domains. In COS-7 cells transfected with rat EP2 cDNA, specific [3H]PGE2 binding was found with a dissociation constant of 14.9 nM, and this binding was inhibited by unlabeled PGE2 and PGF2α. PGE2 and butaprost, an EP2 selective agonist, were effective in increasing the cAMP level in the COS-7 cell transfectants. Northern blot and RT-PCR analysis showed widespread distribution of the EP2 receptor in various tissues. Higher EP2 expression was found in fetal long bones and calvariae than in adult by RT-PCR and in situ hybridization, suggesting a role for this receptor in rapidly growing skeletal tissue.</div>","PeriodicalId":20653,"journal":{"name":"Prostaglandins","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0090-6980(97)00145-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91772906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 76

Tumor Necrosis Factor-α Promotes Sustained Cyclooxygenase-2 Expression: Attenuation by Dexamethasone and NSAIDs 肿瘤坏死因子-α促进环氧化酶-2的持续表达:地塞米松和非甾体抗炎药的抑制

Prostaglandins Pub Date : 1997-10-01 DOI: 10.1016/S0090-6980(97)00144-5

Douglas J Perkins , Douglas A Kniss

{"title":"Tumor Necrosis Factor-α Promotes Sustained Cyclooxygenase-2 Expression: Attenuation by Dexamethasone and NSAIDs","authors":"Douglas J Perkins , Douglas A Kniss","doi":"10.1016/S0090-6980(97)00144-5","DOIUrl":"https://doi.org/10.1016/S0090-6980(97)00144-5","url":null,"abstract":"<div>Prostaglandin (PG) release is characteristic of most inflammatory diseases. The committed step in the formation of free arachidonic acid into PG products is catalyzed by cyclooxygenase (COX, prostaglandin H2 synthase, PGHS), which exists as two genetically distinct isoforms. COX-1 is constitutively expressed and produces PGs and thromboxane A2 during normal physiologic activities, while COX-2 is an inducible enzyme stimulated by growth factors, lipopolysaccharide, and cytokines during inflammation or cell injury. Proinflammatory cytokines such as tumor necrosis factor-α (TNF-α) released into the amniotic fluid in the setting of infection have been proposed to signal amnion and decidual cells to produce PGs that may culminate in preterm labor. However, since the molecular control of this phenomenon has not been established, this study used amnion-derived WISH cells to determine if TNF-α promoted the formation of PGs through COX-2 activity. Treatment of WISH cells with TNF-α (0.1 ng/mL–100 ng/mL) caused a dose-dependent increase in COX-2 expression and the subsequent biosynthesis of PGE2 that persisted for at least 48 hrs. In contrast, COX-1 mRNA and protein levels were unaltered by TNF-α treatment as determined by RT-PCR and immunoblot analysis, respectively. TNF-α-stimulated COX-2 expression and the subsequent formation of PGE2 were inhibited by dexamethasone (0.1 μM). In addition, indomethacin (1 μM) and the novel COX-2-selective inhibitor, NS-398 (IC50 ∼ 1.1 × 10−9 M), attenuated TNF-α-elicited PGE2 production. Results presented here demonstrate that TNF-α elicits prolonged and regulatable induction of COX-2 in WISH cells, while COX-1 is constitutively expressed and unchanged in response to TNF-α stimulation.</div>","PeriodicalId":20653,"journal":{"name":"Prostaglandins","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0090-6980(97)00144-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91772907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 104

The Contribution of Synovial Fluid Lipoproteins to the Chronic Synovitis of Rheumatoid Arthritis 滑膜液脂蛋白在类风湿性关节炎慢性滑膜炎中的作用

Prostaglandins Pub Date : 1997-10-01 DOI: 10.1016/S0090-6980(97)00147-0

Pamela E Prete M.D. , Arzu Gurakar-Osborne Ph.D.

引用次数: 29

Kinetics of Endogenous Leukotriene B4 and E4 Production Following Injection of the Chemotactic Peptide FMLP in the Rabbit 兔内源性白三烯B4和E4产化趋化肽FMLP的动力学研究

Prostaglandins Pub Date : 1997-10-01 DOI: 10.1016/S0090-6980(97)00146-9

Antonio Celardo , Giuseppe Dell’Elba , Stefano Manarini , Virgilio Evangelista , Giovanni de Gaetano , Chiara Cerletti

{"title":"Kinetics of Endogenous Leukotriene B4 and E4 Production Following Injection of the Chemotactic Peptide FMLP in the Rabbit","authors":"Antonio Celardo , Giuseppe Dell’Elba , Stefano Manarini , Virgilio Evangelista , Giovanni de Gaetano , Chiara Cerletti","doi":"10.1016/S0090-6980(97)00146-9","DOIUrl":"10.1016/S0090-6980(97)00146-9","url":null,"abstract":"<div>The kinetic profiles of leukotriene B4 (LTB4) and E4 (LTE4) after intravenous administration (30 nmol/kg) of the inflammatory peptide N-formyl-l-methionyl-l-leucyl-l-phenylalanine (FMLP) were evaluated in male rabbits. LTB4 and LTE4 reached the maximal concentration of 84.2 ± 60.0 and 162.2 ± 51.4 nmol/L (mean ± s.d.), at 2 and 5 min, respectively. The first elimination phase for LTB4 and LTE4, after FMLP administration, showed an apparent half-life of 24.6 ± 6.7 and 36.9 ± 13.0 min, respectively. The area under the blood concentration-time curve (AUC, nmol min/L) of LTB4 and LTE4 was 2178 ± 1591 and 7627 ± 3052, respectively. LTE4 and N-ac-LTE4 were the major components excreted in the urine, mostly in the first time interval (0–12 h) of urinary collection after FMLP treatment; 11-trans-LTE4 was recovered in the second interval (12–24 h). Two other more polar compounds, potential metabolites, were recovered in the first interval of urine collection. Knowledge of the kinetic characteristics of endogenously produced leukotrienes may be useful in understanding the role of these eicosanoids in inflammatory and thrombotic disease, as well as in evaluating the efficacy of drugs designed to modulate their production and effect.</div>","PeriodicalId":20653,"journal":{"name":"Prostaglandins","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0090-6980(97)00146-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20367307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Cyclooxygenases and the Central Nervous System 环氧化酶和中枢神经系统

Prostaglandins Pub Date : 1997-09-01 DOI: 10.1016/S0090-6980(97)00128-7

Walter E. Kaufmann , Katrin I. Andreasson , Peter C. Isakson , Paul F. Worley

引用次数: 262

Measurements of Urinary Prostaglandins in Young Ovulatory Women During the Menstrual Cycle and in Postmenopausal Women 年轻排卵期妇女月经周期和绝经后妇女尿前列腺素的测定

Prostaglandins Pub Date : 1997-09-01 DOI: 10.1016/S0090-6980(97)00131-7

K Farker, H Schweer , R Vollandt , N Nassr, U Nagel, H.W Seyberth , A Hoffmann, M Oettel

{"title":"Measurements of Urinary Prostaglandins in Young Ovulatory Women During the Menstrual Cycle and in Postmenopausal Women","authors":"K Farker, H Schweer , R Vollandt , N Nassr, U Nagel, H.W Seyberth , A Hoffmann, M Oettel","doi":"10.1016/S0090-6980(97)00131-7","DOIUrl":"10.1016/S0090-6980(97)00131-7","url":null,"abstract":"<div>The purpose of the present work was to study the prostaglandin excretion in young nonpregnant ovulatory women during the menstrual cycle on the one hand and in postmenopausal women on the other hand and to investigate the influence of female sex hormones (estradiol, progesterone) on urinary prostanoid excretion. Urinary excretion rates of prostaglandin E2 (PGE2), 6-keto-PGF1α, thromboxane B2 (TxB2) and their metabolites PGE-M (11α-hydroxy-9,15-dioxo-2,3,4,5,20-pentanor-19-carboxyprostanoic acid), 2,3-dinor-6-keto-PGF1α, 2,3-dinor-TxB2 and 11-dehydro-TxB2 were determined by gas chromatography-triple stage quadrupole mass spectrometry (GC/MS/MS) in 41 young nonpregnant women during the follicular phase and during the luteal phase and in 23 postmenopausal women. Excretion rates of all urinary prostanoids were not significantly different in the follicular phase when compared with the luteal phase. In contrast to the young ovulatory women, PGE2 and TxB2 were significantly higher in postmenopausal women. Concerning the other prostaglandins significant differences between these groups of women did not exist. Although serum levels of estradiol and progesterone were different in young and postmenopausal women, sex hormones have not been shown to correlate with prostaglandins. Our data do not suggest sex hormones to be responsible for the difference in the prostaglandin excretion in women of reproductive age and in women in the menopause. Further systematic investigations into age dependency of prostaglandin excretion in women are necessary.</div>","PeriodicalId":20653,"journal":{"name":"Prostaglandins","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0090-6980(97)00131-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20305595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6