1st Portuguese Biomedical Engineering Meeting最新文献

筛选
英文 中文
Role of tyrosine phosphorylation in the regulation of Burkholderia cell physiology 酪氨酸磷酸化在伯克氏菌细胞生理调节中的作用
1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026056
A. S. Ferreira, I. N. Silva, J. Becker, L. M. Moreira
{"title":"Role of tyrosine phosphorylation in the regulation of Burkholderia cell physiology","authors":"A. S. Ferreira, I. N. Silva, J. Becker, L. M. Moreira","doi":"10.1109/ENBENG.2011.6026056","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026056","url":null,"abstract":"Burkholderia cepacia complex bacteria are opportunistic pathogens, causing severe lung infections in cystic fibrosis patients. Many clinical isolates produce the exopolysaccharide cepacian, believed to be an infection persistence determinant. Little is known about cepacian biosynthesis regulation, but our studies showed the importance of post-translational mechanisms mediated by tyrosine phosphorylation, namely through the tyrosine autokinase BceF, which affects EPS biosynthesis. Indeed, insertion mutants on bceF and bceE genes, this last one encoding an outer membrane porin, caused an exopolysaccharide deficient phenotype, confirming their essential role in EPS biosynthesis. Assessment of biofilm formation showed a stronger reduction in biofilm thickness by the bceF mutant when compared to bceE mutant and the parental strain B. cepacia IST408. Transcriptome analysis suggests that BceF may regulate other cellular functions as indicated by the high number of differentially expressed genes when its transcriptome was compared to the one of the parental strain and the bceE mutant.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123512468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Pervasive sensing and computing for wheelchairs users health assessment 用于轮椅使用者健康评估的普适传感和计算
1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026070
O. Postolache, P. Girão, E. Pinheiro, M. Pereira, R. Madeira, J. Mendes, Manuel Cunha, G. Postolache, Claudia Maia Moura
{"title":"Pervasive sensing and computing for wheelchairs users health assessment","authors":"O. Postolache, P. Girão, E. Pinheiro, M. Pereira, R. Madeira, J. Mendes, Manuel Cunha, G. Postolache, Claudia Maia Moura","doi":"10.1109/ENBENG.2011.6026070","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026070","url":null,"abstract":"Optimal cardiovascular, respiratory and motor activity assessment of wheelchair users using modern technologies of pervasive sensing and computing represents the main objective of the running project. In order to extract accurate information about physiological parameters provided by physical measurement channel and also to estimate additional parameters associated with virtual measurement channels advanced processing algorithms are implemented on embedded computing platform of the wheelchair. Will be implemented ballistocardiography, electrocardiography, plethysmography, skin conductance smart sensing devices that extract the health status information in unobtrusive way. The informations from the smart sensors, as from the localization units based on GPS technology (for outdoor localization) and RFID technology (for indoor localization) are processed on the embedded computing platform based on DSP that also assure the wireless data transmission to the human machine interface (HMI) expressed by a tablet that run mobile OS such as Android or Windows Mobile. A web based information system is used as part of the electronic health record that assure the remote health care assistance of the wheelchair users.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121051220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Ex-vivo expansion of hematopoietic stem cells from umbilical cord blood 脐带血造血干细胞的体外扩增
1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026052
C. L. da Silva, Pedro Z. Andrade, Francisco dos Santos, J. Cabral
{"title":"Ex-vivo expansion of hematopoietic stem cells from umbilical cord blood","authors":"C. L. da Silva, Pedro Z. Andrade, Francisco dos Santos, J. Cabral","doi":"10.1109/ENBENG.2011.6026052","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026052","url":null,"abstract":"One of the major focuses in experimental hematology is the in vitro manipulation of hematopoietic stem cells (HSC) and progenitors with the goal of generating clinically relevant cell numbers from the limited number of cells present in available samples for multiple settings including bone marrow transplantation, somatic cell gene therapy and production of mature blood cell types. The goal of ex-vivo expansion is to induce proliferation of stem/progenitor cells, while maintaining their primary functional characteristics, namely, their ability to sustain long-term hematopoiesis in vivo. Although the hematopoietic system is one of the most intensively studied, with numerous clinical applications, it is still far from being completely understood, and hematopoietic cell culture remains among the most challenging culture systems due to its intrinsic heterogeneity and high variability derived from the cell source. The ability to successfully expand the numbers of human HSC in highly controlled culture systems (i.e. bioreactors) would clearly boost their current and future medical use.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125538420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Computational modelling in bone mechanics 骨力学中的计算模型
1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026079
P. Fernandes, M. Dias
{"title":"Computational modelling in bone mechanics","authors":"P. Fernandes, M. Dias","doi":"10.1109/ENBENG.2011.6026079","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026079","url":null,"abstract":"Bone tissue is a natural structural material that is able to adapt its form to the function. Thus, a mathematical description of the functional adaptation of bone is essential not only to understand the bone adaptive behaviour but also to build models that are able to support the design of new bone implants and bone scaffolds for tissue engineering and to help in disease diagnostic. This paper describes the research efforts, performed at the Institute of Mechanical Engineering (IDMEC-IST), in order to achieve mathematical and computational models to describe the bone behaviour and its response to the mechanical environment. A computational bone remodelling model is briefly described as well as its application to bone tissue engineering and implant design. It shows the role that computational tools can play in the development of new medical devices and to support pre-clinical evaluation.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130268134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Reconstruction of the evolutionary history of the DHA1 family of yeast multidrug resistance transporters of the major facilitator superfamily 酵母多药耐药转运体DHA1家族主要促进剂超家族的进化历史重建
1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026075
P. Dias, I. Sá-Correia
{"title":"Reconstruction of the evolutionary history of the DHA1 family of yeast multidrug resistance transporters of the major facilitator superfamily","authors":"P. Dias, I. Sá-Correia","doi":"10.1109/ENBENG.2011.6026075","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026075","url":null,"abstract":"Multidrug resistance (MDR) is frequently mediated by the action of drug-efflux pumps, which are able to catalyze the active expulsion of several unrelated cytotoxic compounds out of the cell or their intracellular partitioning. A group of transporters involved in MDR belongs to the major facilitator superfamily (MFS) which, based on protein topology, phylogeny and function, divides into two families: 12-spanner drug:H+ antiporter-1 (DHA1) and 14-spanner drug:H+ antiporter-2 (DHA2). The evolutionary history of 422 DHA1 proteins identified in twenty-four hemiascomycetous genomes was reconstructed. The phylogenetic tree divided the DHA1 genes into 21 clusters, twelve containing no Saccharomyces cerevisiae members. Inclusion of gene neighbourhood data allowed to scrutinize the orthologous, paralogous, and ohnologous status of these genes, and also to identify ten main DHA1 gene lineages, spanning the whole hemiascomycetes clade. The DHA1 proteins of seven pathogenic Candida spp. were phylogenetically characterized.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"102 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122608832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification and exploitation of Burkholderia cepacia complex virulence factors as potential antimicrobial targets 洋葱伯克氏菌复合毒力因子作为潜在抗菌靶点的鉴定与开发
1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026036
S. A. Sousa, C. G. Ramos, J. Feliciano, J. Leitão
{"title":"Identification and exploitation of Burkholderia cepacia complex virulence factors as potential antimicrobial targets","authors":"S. A. Sousa, C. G. Ramos, J. Feliciano, J. Leitão","doi":"10.1109/ENBENG.2011.6026036","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026036","url":null,"abstract":"A multidisciplinary approach is being used to identify and validate virulence factors and determinants of bacteria of the Burkholderia cepacia complex (Bcc). Bcc is a group of problematic opportunistic pathogenic bacteria, particularly among cystic fibrosis patients, due to their easy transmissibility, resistance to multiple antibiotics and potential to cause a fatal necrotizing pneumonia. Genes encoding a Type II phosphomannose isomerase (BceAJ), an outer membrane protein A (Bcn-OmpA), and an acyl carrier protein (ACP) were already cloned and functionally characterized, envisaging their exploitation as new targets for the rational design of novel therapeutic strategies to fight infections caused by these emergent multi-drug resistant human pathogens.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117002037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic engineering of stem cells by non-viral vectors 非病毒载体干细胞的基因工程
1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026044
C. Madeira, S. Ribeiro, R. Mendes, Irina Pinheiro, C. L. da Silva, J. Cabral
{"title":"Genetic engineering of stem cells by non-viral vectors","authors":"C. Madeira, S. Ribeiro, R. Mendes, Irina Pinheiro, C. L. da Silva, J. Cabral","doi":"10.1109/ENBENG.2011.6026044","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026044","url":null,"abstract":"Stem/progenitor cells hold a great promise for application in several therapies due to their unique biological characteristics. With the purpose of harnessing these cells full potential in cell-or gene-based therapies it might be advantageous to enhance some of their features through gene delivery strategies. Accordingly, we are interested in developing efficient and safe methodologies to genetically engineer stem cells, boosting their therapeutic efficacy in Regenerative Medicine. In our work, delivery of plasmid DNA to human Bone Marrow Mesenchymal Stem Cells (BM-MSC) was optimized by lipofection and by a recently available microporation technique and no effect was observed in their immunophenotypic characteristics or differentiative potential. After lipofection similar number of plasmid copies was determined at different cell passages. Importantly, cell proliferation kinetics slowed down due to the presence of plasmid. Overall, we believe our findings are extremely useful towards the maximization of gene delivery to human MSC, without compromising cell function and viability.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"9 32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129104402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
OMICS approaches to reveal Burkholderia cenocepacia adaptive strategies to long-term residence in the lungs of cystic fibrosis patients under antibiotic therapy 组学方法揭示囊性纤维化患者在抗生素治疗下肺部长期居住的伯克霍尔德菌适应策略
1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026074
Andreia Madeira, N. Mira, P. Santos, C. Coutinho, Ana Pinto-de-Oliveira, A. Moreira, C. Roma-Rodrigues, I. Sá-Correia
{"title":"OMICS approaches to reveal Burkholderia cenocepacia adaptive strategies to long-term residence in the lungs of cystic fibrosis patients under antibiotic therapy","authors":"Andreia Madeira, N. Mira, P. Santos, C. Coutinho, Ana Pinto-de-Oliveira, A. Moreira, C. Roma-Rodrigues, I. Sá-Correia","doi":"10.1109/ENBENG.2011.6026074","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026074","url":null,"abstract":"Burkholderia cepacia complex (Bcc) bacteria are serious opportunistic pathogens, especially in immunocompromised and cystic fibrosis (CF) patients. The first epidemiological survey of Bcc bacteria involved in pulmonary infections among the CF population under surveillance at the major Portuguese CF Treatment Center at Santa Maria Hospital was published in 2000. Based on a collection of clinical isolates gathered during the epidemiological surveys and on indications from molecular microbiology studies, we are exploiting OMICS approaches (quantitative proteomics based on 2D-Difference Gel Electrophoresis — 2D-DIGE — and transcriptomic analysis) to compare the genomic expression programs of clonal isolates retrieved from chronically infected CF patients. The proteomes and the transcriptomes of the first B. cenocepacia isolate recovered from a CF patient and a clonal variant obtained after 3 years of persistent infection and following intravenous antibiotic therapy, were recently compared. Results are providing clues on molecular mechanisms underlying bacterial adaptation and resistance to the CF airways stressing environment, to host defense mechanisms and to therapeutically administered antibiotics.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115734255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Towards a high-throughput drug discovery platform for the screening of GPCR targets in cells 迈向细胞中筛选GPCR靶点的高通量药物发现平台
1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026042
D. Prazeres, S. Martins, J. R. Trabuco, G. Monteiro, A. Juskowiak, J. Conde, V. Chu
{"title":"Towards a high-throughput drug discovery platform for the screening of GPCR targets in cells","authors":"D. Prazeres, S. Martins, J. R. Trabuco, G. Monteiro, A. Juskowiak, J. Conde, V. Chu","doi":"10.1109/ENBENG.2011.6026042","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026042","url":null,"abstract":"G-protein-coupled receptors (GPCRs) play a key role in physiologic and pathologic processes via signal transduction pathways that convert extracellular information into intracellular functions. The identification of drugs that target GPCRs usually relies on functional, cell-based assays. Here, the binding of a test compound to a receptor alters the conformation of the associated G-protein, initiating a cascade of events. The magnitude of the response is typically measured by monitoring key elements in the cascade (e.g., calcium) with fluorescent dyes or with luminescent reporter proteins (e.g., aequorin). The goal of this project is to develop cell microchips for the screening of drugs that act upon GPCRs. The basic unit of the chip will be composed of: i) 15–100 micron-sized test sites where the cells are placed and contacted with the candidate drug via a microfluidic network, and ii) independent, micron-sized amorphous silicon photodetectors that convert the photons of generated light into an electric current and that can be arrayed.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115758392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
neuroCornea — Diabetic peripheral neuropathy early diagnosis and follow-up through in vivo automatic analysis of corneal nerves morphology 神经角膜-通过角膜神经形态的体内自动分析,糖尿病周围神经病变的早期诊断和随访
1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026071
Ana Ferreira, João Lamas, L. Gomes, S. Silva, C. Loureiro, J. Domingues, J. S. Silva, M. Morgado
{"title":"neuroCornea — Diabetic peripheral neuropathy early diagnosis and follow-up through in vivo automatic analysis of corneal nerves morphology","authors":"Ana Ferreira, João Lamas, L. Gomes, S. Silva, C. Loureiro, J. Domingues, J. S. Silva, M. Morgado","doi":"10.1109/ENBENG.2011.6026071","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026071","url":null,"abstract":"Peripheral diabetic neuropathy is the major cause of chronic disability in diabetic patients. The early diagnosis and accurate assessment of peripheral neuropathy are important to define the higher risk patients, decrease patient morbidity and assess the performance of new therapies. However, the peripheral neuropathy diagnosis often fails or occurs only when patients became symptomatic due to the non-availability of a simple non invasive method for early diagnosis. Corneal confocal microscopy is a non-invasive imaging modality that can document corneal nerves morphology. In this project, we will develop a technique for early diagnosis of diabetic neuropathy based on automatic analysis of corneal nerves images. This project comprises the development of automatic algorithms for nerve segmentation and morphometric parameters extraction, the evaluation of the best parameters for early diagnosis and follow-up of diabetic neuropathy and the development of a corneal confocal imaging module to be used as an add-on to a standard slit-lamp.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"2017 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114194014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信