Proceedings of the National Academy of Sciences of the United States of America最新文献_第5页

Compensate peer reviewers; do not conscript them. 补偿同行审稿人；不要征召他们。

IF 9.5 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2026-10-27 Epub Date: 2026-10-16 DOI: 10.1073/pnas.2619714123

Daniel A Gorelick, Jonas Kunst

引用次数: 0

Identification and characterization of neoplastic cells in keratin-positive giant cell-rich tumors. 角蛋白阳性富巨细胞肿瘤中肿瘤细胞的鉴定和表征。

IF 9.5 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2026-10-27 Epub Date: 2026-10-19 DOI: 10.1073/pnas.2611959123

Maarten van der Linde, John S A Chrisinger, Elizabeth G Demicco, Carina A Dehner, Gregory W Charville, Inge H Briaire-de Bruijn, Sushama Varma, ChunFang Zhu, Magdalena Matusiak, Judith V M G Bovée, Matt van de Rijn, David Gp van IJzendoorn

{"title":"Identification and characterization of neoplastic cells in keratin-positive giant cell-rich tumors.","authors":"Maarten van der Linde, John S A Chrisinger, Elizabeth G Demicco, Carina A Dehner, Gregory W Charville, Inge H Briaire-de Bruijn, Sushama Varma, ChunFang Zhu, Magdalena Matusiak, Judith V M G Bovée, Matt van de Rijn, David Gp van IJzendoorn","doi":"10.1073/pnas.2611959123","DOIUrl":"https://doi.org/10.1073/pnas.2611959123","url":null,"abstract":"Keratin-positive giant cell-rich tumor (KPGCT) is a newly described bone and soft tissue tumor. The tumor is characterized by scattered keratin-positive cells and the presence of HMGA2::NCOR2 fusions. It is not known if the HMGA2::NCOR2 fusion is located in the keratin-positive cells, and little is known about how KPGCT develops. KPGCT shares some histologic features with tenosynovial giant cell tumor (TGCT), a soft tissue tumor with CSF1 rearrangements. Single-nuclei RNA sequencing and Xenium spatial transcriptomics were used to elucidate the most likely mechanisms driving KPGCT and compare KPGCT to TGCT. We identified a population of cells coexpressing keratin, HMGA2, and CSF1 as the likely neoplastic cells; these cells constitute the minority of the cells in the tumor. These cells express no synovial markers, confirming KPGCT as a distinct entity, separate from TGCT. The bulk of the tumor consists of CSF1R-expressing macrophages and multinucleated giant cells (MNGCs), suggesting an important role for CSF1-CSF1R signaling. In addition, we find that the likely neoplastic cell population shows activation of the hippo signaling pathway, a possible central pathway in KPGCT tumorigenesis. The CSF1-CSF1R axis likely stimulates the migration and proliferation of macrophages, which form the majority of cells in the tumor, as well as their differentiation into MNGCs. These results provide a rationale for the use of CSF1R inhibitors, which have already shown efficacy in TGCT, as a therapy for KPGCT.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"123 43","pages":"e2611959123"},"PeriodicalIF":9.5,"publicationDate":"2026-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understory diversity is positively associated with overstory carbon sequestration. 林下多样性与林下碳固存呈正相关。

IF 9.5 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2026-10-27 Epub Date: 2026-10-19 DOI: 10.1073/pnas.2615624123

Yakun Zhang, Rui Zhang, Scott X Chang, Han Y H Chen, Anthony R Taylor, Masumi Hisano, Chen Chen, Sai Peng, Ya Wang, Xin Chen, Jiasheng Wu, Xinli Chen, Peter B Reich

{"title":"Understory diversity is positively associated with overstory carbon sequestration.","authors":"Yakun Zhang, Rui Zhang, Scott X Chang, Han Y H Chen, Anthony R Taylor, Masumi Hisano, Chen Chen, Sai Peng, Ya Wang, Xin Chen, Jiasheng Wu, Xinli Chen, Peter B Reich","doi":"10.1073/pnas.2615624123","DOIUrl":"https://doi.org/10.1073/pnas.2615624123","url":null,"abstract":"Forests are a major terrestrial carbon sink, and higher overstory diversity is often associated with greater carbon accrual in trees and soils. Yet the contribution of the understory vegetation, the most species-rich forest stratum, to tree and soil carbon accumulation remains largely unknown. Here, we analyzed Canada's National Forest Inventory database to explore whether understory diversity (richness and evenness of shrubs, herbs, and bryophytes) is associated with carbon accumulation in overstory trees and soils across environmental gradients of air temperature, climate moisture, and nitrogen deposition. We found that herb and bryophyte species richness were positively associated with decadal tree carbon accumulation, with the strongest effects at warmer, drier, and nitrogen-limited sites. In contrast, understory diversity showed no consistent association with soil carbon accumulation. On a decadal scale, increasing herb and bryophyte species richness from their minimum to median values corresponded to 15% and 6% increases in tree carbon accumulation, respectively. These results provide large-scale observational evidence that diversity in lower vegetation layers is positively associated with overstory carbon sequestration and reveal strong environmental modulation of these multistratal biodiversity-ecosystem functioning relationships. These findings suggest that species-rich herb and bryophyte communities should be considered in strategies for sustaining forest carbon sinks, especially under warmer, drier conditions and nutrient limitation.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"123 43","pages":"e2615624123"},"PeriodicalIF":9.5,"publicationDate":"2026-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Climate-driven rodent demography provides a general, transferable basis for predicting arenavirus spillover. 气候驱动的啮齿动物人口学为预测沙粒病毒外溢提供了一个通用的、可转移的基础。

IF 9.5 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2026-10-27 Epub Date: 2026-10-19 DOI: 10.1073/pnas.2631478123

John M Drake, Nia W Carroll

引用次数: 0

Biotic response networks are an important organizer of the transcriptome in wild Arabidopsis thaliana populations. 生物响应网络是野生拟南芥群体转录组的重要组织者。

IF 9.5 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2026-10-27 Epub Date: 2026-10-19 DOI: 10.1073/pnas.2611539123

Ana Paula Leite Montalvão, Kevin D Murray, Ilja Bezrukov, Natalie Betz, Lucas P Henry, Paloma Duran, Patrick Boppert, Martina Kolb, Fabrice Roux, Joy Bergelson, Detlef Weigel, Wei Yuan

{"title":"Biotic response networks are an important organizer of the transcriptome in wild Arabidopsis thaliana populations.","authors":"Ana Paula Leite Montalvão, Kevin D Murray, Ilja Bezrukov, Natalie Betz, Lucas P Henry, Paloma Duran, Patrick Boppert, Martina Kolb, Fabrice Roux, Joy Bergelson, Detlef Weigel, Wei Yuan","doi":"10.1073/pnas.2611539123","DOIUrl":"https://doi.org/10.1073/pnas.2611539123","url":null,"abstract":"Extensive laboratory experimentation has revealed conserved molecular pathways controlling growth and stress responses in plants, yet how these programs operate in natural settings remains poorly understood. We have investigated the organization of the transcriptome in wild populations of Arabidopsis thaliana by sampling plants across 60 natural sites in Europe and North America over two seasons. Transcriptomes varied extensively among individuals and were only weakly structured by geography or season. No groups of plants stood out due to clear biotic or abiotic stress signatures known from the laboratory. While only a small portion of transcriptome variance was explained by disease symptoms or environmental and phenotypic variables, infection-associated signals accounted for the largest share of the explainable fraction. Biotic-response networks identified in controlled laboratory conditions were well conserved in wild transcriptomes, while other networks, including abiotic-response networks were substantially reorganized in wild plants. Together, these results indicate that while certain transcriptional modules are easily reidentified when moving from the wild to controlled conditions, many regulatory relationships differ in these two contexts, highlighting how ecological complexity shapes transcriptome organization in natural populations.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"123 43","pages":"e2611539123"},"PeriodicalIF":9.5,"publicationDate":"2026-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mediator-associated proteins and the small RNA-guided Polycomb repressive complex enhance cryptic transcription for transposon exclusion. 介质相关蛋白和小rna引导的Polycomb抑制复合物增强了转座子排除的隐转录。

IF 9.5 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2026-10-27 Epub Date: 2026-10-20 DOI: 10.1073/pnas.2606005123

Zhihao Zhai, Haoyu He, Tomoko Noto, Mingmei Liucong, Jialuo Li, Julie Saksouk, Yike Liu, Xue Wang, Yujie Liu, Kazufumi Mochizuki, Miao Tian

{"title":"Mediator-associated proteins and the small RNA-guided Polycomb repressive complex enhance cryptic transcription for transposon exclusion.","authors":"Zhihao Zhai, Haoyu He, Tomoko Noto, Mingmei Liucong, Jialuo Li, Julie Saksouk, Yike Liu, Xue Wang, Yujie Liu, Kazufumi Mochizuki, Miao Tian","doi":"10.1073/pnas.2606005123","DOIUrl":"https://doi.org/10.1073/pnas.2606005123","url":null,"abstract":"Suppression of transposable elements (TEs) is critical for maintaining genome integrity. The evolutionary arms race between TEs and their eukaryotic host organisms has driven the development of small RNA-dependent mechanisms that specifically suppress TEs while leaving non-TE regions unperturbed. In the binucleated ciliate Tetrahymena, small RNAs (sRNAs) are generated from both TE and non-TE regions of the germline genome, whereas noncoding RNAs (ncRNAs) from the TE-free somatic genome target and eliminate non-TE sRNAs, thereby selectively preserving TE-specific sRNAs for guiding TE recognition. Since sRNAs are produced from both strands, the counteracting ncRNAs must also occur bidirectionally. However, the mechanism by which the somatic genome generates ncRNAs from both strands remains unresolved. Here, we show that these ncRNAs arise from widespread cryptic transcription initiated outside canonical promoters and dependent on the developmentally specific Mediator complex-associated proteins Dima1 and Emit2. We further demonstrate that sRNA-guided recruitment of the Tetrahymena Polycomb repressive complex 2 and Dima1 occupancy are mutually reinforcing, promoting local accumulation of RNA polymerase II, which may facilitate cryptic transcription from these loci in a histone methyltransferase-independent manner. Disruption of Dima1 abolishes cryptic transcription, leads to aberrant accumulation of non-TE sRNAs, and results in erroneous genome elimination and cell death. These findings reveal a regulatory circuit involving sRNAs, Polycomb, and the Mediator-associated protein Dima1 that promotes cryptic transcription, thereby ensuring accurate TE exclusion and genome integrity.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"123 43","pages":"e2606005123"},"PeriodicalIF":9.5,"publicationDate":"2026-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149228664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

mechanoLight: A genetically encoded fluorescent biosensor for visualizing 5-HT2AR mechanotransduction in live cells. mechanoLight：一种基因编码荧光生物传感器，用于可视化活细胞中5-HT2AR的机械转导。

IF 9.5 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2026-10-27 Epub Date: 2026-10-19 DOI: 10.1073/pnas.2610816123

In-Yeop Baek, Hyunbin Kim, Duwan Kwon, Hahnbeom Park, Jihye Seong

{"title":"mechanoLight: A genetically encoded fluorescent biosensor for visualizing 5-HT2AR mechanotransduction in live cells.","authors":"In-Yeop Baek, Hyunbin Kim, Duwan Kwon, Hahnbeom Park, Jihye Seong","doi":"10.1073/pnas.2610816123","DOIUrl":"https://doi.org/10.1073/pnas.2610816123","url":null,"abstract":"G protein-coupled receptors (GPCRs) are pivotal sensors of the mechanical environment, yet how they sense and transduce physical stimuli into biochemical signals within live cells remains largely elusive. Here we introduce mechanoLight, a genetically encoded red fluorescent biosensor designed to report the mechanical activation of the 5-hydroxytryptamine 2A receptor (5-HT2AR). By incorporating a circularly permuted mApple (cp-mApple) into the third intracellular loop of 5-HT2AR with the optimized linker (m-linker), mechanoLight selectively detects conformational changes of 5-HT2AR in response to fluid shear stress while exhibiting minimal sensitivity to 5-HT. In sharp contrast, psychLight2, the previously developed green 5-HT2AR biosensor, primarily detects ligand-induced 5-HT2AR activation, suggesting that mechanoLight and psychLight2 capture distinct conformational modes of 5-HT2AR activation. Integrated analysis of linker-swapping experiments, structural modeling, and molecular dynamics simulations revealed that the flexibility of the m-linker underlies the mechanosensitivity of mechanoLight. We further extend this strategy to other mechanosensitive GPCRs, enabling visualization of their mechanoreactivity in live cells. The mechanoLight signal reflects the mechanosensitivity of 5-HT2AR, as shear stress-induced 5-HT2AR activation triggers Gαq-dependent signaling pathways, including phospholipase C (PLC) activation, intracellular Ca2+ mobilization, and ERK phosphorylation. Mutational analysis further identifies N-terminal glycosylation and helix 8 as structural determinants of 5-HT2AR mechanotransduction. Together, our findings establish 5-HT2AR as a mechanotransducer and introduce mechanoLight as a powerful tool for deciphering the molecular principles of GPCR mechanotransduction in live cells.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"123 43","pages":"e2610816123"},"PeriodicalIF":9.5,"publicationDate":"2026-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calibration, accuracy, and domain variation across one million collective forecasts from prediction platforms. 来自预测平台的100万个集体预测的校准、准确性和域变化。

IF 9.5 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2026-10-27 Epub Date: 2026-10-19 DOI: 10.1073/pnas.2615627123

Justin Dickerson, Hilla Shinitzky, Maayan Peer, Mattan S Ben-Shachar, Michael Gilead

引用次数: 0

Scientists disrupt less but more persistently with career aging. 科学家在职业老龄化方面的干扰较少，但更持久。

IF 9.5 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2026-10-27 Epub Date: 2026-10-19 DOI: 10.1073/pnas.2626763123

Nan Deng, Liying Yang, Shlomo Havlin, An Zeng

{"title":"Scientists disrupt less but more persistently with career aging.","authors":"Nan Deng, Liying Yang, Shlomo Havlin, An Zeng","doi":"10.1073/pnas.2626763123","DOIUrl":"https://doi.org/10.1073/pnas.2626763123","url":null,"abstract":"Disruption describes the extent to which a scientific work overturns the prior knowledge on which it builds and leads to a new line of research. A scientist's tendency to produce disruptive work has been found recently to decline over the career. However, the disruption index captures only how strongly a paper overturns prior work and ignores whether that disruption is later accepted or overturned by subsequent research. The persistent disruption (PD) framework adds this second perspective, identifying work that disrupts prior research and is then consolidated rather than displaced by later work. We examine how PD evolves over the career and find the opposite pattern: The probability of producing a persistent disruptive paper increases with career age. Senior scientists disrupt prior work less often compared to young, but once they do, the disruption tends to persist rather than being quickly superseded. Our results suggest that this reversal feature is due to the experience and we corroborate it across several dimensions of recognition. We also show that, at a fixed level of disruptiveness, the more senior the scientist is, it attracts more citations, social-media attention, and research grants. Nobel laureates also show delayed persistent disruptions. They exhibit high disruptive ability early in their careers, yet their prize-winning work appears years later. This lag further indicates that experience is needed to turn disruptive ability into the field's most celebrated contributions.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"123 43","pages":"e2626763123"},"PeriodicalIF":9.5,"publicationDate":"2026-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bridging spatial scales in respiratory viral infection. 呼吸道病毒感染的桥接空间尺度。

IF 9.5 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2026-10-27 Epub Date: 2026-10-20 DOI: 10.1073/pnas.2614436123

Ulrik Hvid, Bjarke Frost Nielsen, Kim Sneppen

引用次数: 0