Physiologia Bohemoslovaca最新文献_第3页

Influence of the composition of rat central lymph on the pharmacokinetics (the steady state during infusion, bioavailability, absorption) of diazepam, studied in the blood and lymph. 大鼠中央淋巴组成对地西泮在血液和淋巴中的药代动力学(输注时的稳态、生物利用度、吸收)的影响。

Physiologia Bohemoslovaca Pub Date : 1990-01-01

J Lamka, O Jindrová, S Gallová, R Uhrová, J Kvĕtina

{"title":"Influence of the composition of rat central lymph on the pharmacokinetics (the steady state during infusion, bioavailability, absorption) of diazepam, studied in the blood and lymph.","authors":"J Lamka, O Jindrová, S Gallová, R Uhrová, J Kvĕtina","doi":"","DOIUrl":"","url":null,"abstract":"Diazepam was administered by infusion to three groups of rats with an induced differentiated total lipid content in their lymph (unfed, fed, oil-fed) and its lymph/blood concentration ratios in the steady state were determined. Ratio values were highest in the group with the highest lymph lipid content (the oil-fed group, 2.20 +/- 0.08) and fell significantly in the other groups (fed group 1.46 +/- 0.09, unfed group 1.15 +/- 0.05). The areas under the blood and lymph concentration curves after the intravenous (i.v.) and intraduodenal (i.d.) administration of diazepam were used to determine absolute (F) and lymphatic (FL) bioavailability. The F value in the blood can be raised by increasing the amount of lipids, whereas in the lymph, under the same conditions, it falls. During the i.v. and i.d. administration of diazepam, FL always rises with an increase in the amount of lipids in the lymph. The role played by the lymphatic system in total diazepam absorption was determined from the experimental results of its i.d. administration. The absolute values are very low (0.043-0.316%), but are significantly influenced by the presence of lipids.","PeriodicalId":20547,"journal":{"name":"Physiologia Bohemoslovaca","volume":"39 5","pages":"403-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13304883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of age, sex and hypoxia on plasma dopamine-beta-hydroxylase activity in the rat. 年龄、性别和缺氧对大鼠血浆多巴胺- β -羟化酶活性的影响。

Physiologia Bohemoslovaca Pub Date : 1990-01-01

J Koudelová, J Mourek

{"title":"Influence of age, sex and hypoxia on plasma dopamine-beta-hydroxylase activity in the rat.","authors":"J Koudelová, J Mourek","doi":"","DOIUrl":"","url":null,"abstract":"Using rats (Wistar strain) of our own breed, we studied dopamine-beta-hydroxylase (E.C. 1.14.17.1) (DBH) activity in the plasma of animals of different ages (in correlation to sex) under normal conditions and after exposure to altitude hypoxia (corresponding to 7000 or 9000 m and lasting 20 min). The enzyme was determined by the method of Kato et al. (1974). We found that the given plasma enzyme activity was significantly higher in females than in males, throughout the whole life-span. In addition, we found that minimum activity was reached on about the 14th and 21st day of postnatal life and again on the 40th day, while maximum activity was recorded at the ages of 5, 30 and 35 days and in adult rats. In adult animals (males and females), exposure to altitude hypoxia was followed by a statistically significant increase in plasma DBH activity, which was much more pronounced in females than in males. In males, 240 min after terminating hypoxia plasma DBH activity had returned to normal, but in females it was still significantly raised; after 48 h, plasma DBH activity in females was identical to the activity before exposure to hypoxia. In rats aged 5 and 35 days, hypoxia evoked a fluctuating response. A decrease in activity immediately after terminating hypoxia was followed at 60 min by a return to normal, but at 240 min there was again a significant decrease. In 21-day-old rats, hypoxia did not induce any significant change in plasma DBH activity (the initial activity level in this group was very low).","PeriodicalId":20547,"journal":{"name":"Physiologia Bohemoslovaca","volume":"39 5","pages":"409-16"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13304884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pulmonary endocrine cells and the pulmonary circulation in various species in the Himalaya. 喜马拉雅地区不同物种的肺内分泌细胞和肺循环。

Physiologia Bohemoslovaca Pub Date : 1990-01-01

D Williams

引用次数: 0

Localisation of the origin of self-sustained after-discharges (SSADs) in the rat: the serrated wave (SerW) type of SSAD. 大鼠放电后自我维持(SSAD)起源的定位:SSAD的锯齿波(SerW)型。

Physiologia Bohemoslovaca Pub Date : 1990-01-01

M Pohl, P Mares

引用次数: 0

Time course of lipid peroxidation during incomplete ischaemia followed by reperfusion in rat brain. 大鼠脑不完全缺血再灌注时脂质过氧化的时间过程。

Physiologia Bohemoslovaca Pub Date : 1990-01-01

L Horáková, L Lukovic, V Uraz, S Stolc

引用次数: 0

The isolated perfused heart according to Langendorff technique--function--application. 根据朗根多夫技术-功能-应用的离体灌注心脏。

Physiologia Bohemoslovaca Pub Date : 1990-01-01

H J Döring

{"title":"The isolated perfused heart according to Langendorff technique--function--application.","authors":"H J Döring","doi":"","DOIUrl":"","url":null,"abstract":"1. Definition. The isolated perfused heart according to Langendorff is a preparation which is easily obtained from all warm-blooded animals. The heart is usually perfused at constant pressure, but frequently at constant flow, mostly with oxygenated saline solutions. The ventricles do not fill with the perfusate and therefore do not perform pressure-volume work. If, however, an intraventricular balloon is inserted, the ventricle can contract isovolumetrically. 2. Measuring parameters. Mechanical parameters of the working myocardium (contractile force, volume, ventricular diameter), mean coronary flow, bioelectrical parameters (EKG, monophasic injury potentials), and cardiac rhythm can be measured and recorded. 3. Function tests. The intact function of the working myocardium and the coronary vessels should be examined using various simple tests regarding the myocardium, the smooth musculature, and the endothelium of coronary vessels. 4. Possible applications. In the Langendorff-heart of normal or pretreated animals, inotropic, chronotropic, antiarrhythmic or vasoactive substances can be investigated in the steady state or by means of specific stress tests. In addition, the preparation is particularly suitable for biochemical studies of myocardial metabolism. 6. Advantages. Due to the technical ease with which a variety of parameters can be measured, a single heart can provide maximum information about the condition of the myocardium and the coronary vessels. Thus, it is not surprising to find that 90 years after its first description, the isolated perfused heart according to Langendorff continues to be utilized.","PeriodicalId":20547,"journal":{"name":"Physiologia Bohemoslovaca","volume":"39 6","pages":"481-504"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13258713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The circadian and circa-annual response of rat serum corticosterone to different stressors. 大鼠血清皮质酮对不同应激源的昼夜和年循环反应。

Physiologia Bohemoslovaca Pub Date : 1990-01-01

I Ahlers, E Ahlersová, K Halátová, B Smajda, M Toropila

引用次数: 0

Influence of chronic alterations of salt intake and aging on the kinetic of red cell Na+ and K+ transport in Sprague-Dawley rats. 盐摄入的慢性改变和衰老对Sprague-Dawley大鼠红细胞Na+和K+转运动力学的影响。

Physiologia Bohemoslovaca Pub Date : 1990-01-01

J Zicha, J Duhm

{"title":"Influence of chronic alterations of salt intake and aging on the kinetic of red cell Na+ and K+ transport in Sprague-Dawley rats.","authors":"J Zicha, J Duhm","doi":"","DOIUrl":"","url":null,"abstract":"The kinetics of Na+ and K+ (Rb)+ transport mediated by the Na(+)-K+ pump and Na(+)-K+ cotransport system (assessed as a function of Rb+o and Na+i) as well as the magnitude of cation leaks were determined in red cells of young male rats subjected to chronic salt deprivation or salt loading (0.1% and 8% NaCl diet). These salt intake alterations induced moderate kinetic changes of the Na(+)-K+ pump which did not result in significant changes of ouabain-sensitive (OS) Rb+ uptake or Na+ net extrusion at in vivo Na+i and K+o concentrations because a decreased affinity for Na+i in salt-loaded animals was compensated by an increased maximal transport rate. High furosemide-sensitive (FS) Rb+ uptake in red cells of salt-deprived rats was caused by an increase of both the maximal transport rate and the affinity for Rb+o. Cation leaks were also higher in salt-deprived than in salt-loaded rats. In three age groups of rats fed a 1% NaCl diet FS Rb+ uptake (but not FS Na+ net uptake) rose with age due to an increasing maximal transport rate whereas the affinity of the cotransport system for Rbo+ did not change. The age-dependent changes in the kinetics of the Na(+)-K+ pump resulted in a slight decrease of OS Rb+ uptake with age that was not paralleled by corresponding Na+ net extrusion. No major age-related changes of cation leaks were found. Thus some intrinsic properties of red cell transport systems can be altered by salt intake and aging.","PeriodicalId":20547,"journal":{"name":"Physiologia Bohemoslovaca","volume":"39 1","pages":"37-44"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13296946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of severe incomplete ischaemia and postischaemic reperfusion on phospholipids and unesterified cholesterol in rabbit spinal cord. 严重不完全缺血和缺血后再灌注对兔脊髓磷脂和未酯化胆固醇的影响。

Physiologia Bohemoslovaca Pub Date : 1990-01-01

G Halát, N Lukácová, M Chavko, J Marsala

{"title":"Effect of severe incomplete ischaemia and postischaemic reperfusion on phospholipids and unesterified cholesterol in rabbit spinal cord.","authors":"G Halát, N Lukácová, M Chavko, J Marsala","doi":"","DOIUrl":"","url":null,"abstract":"Effect of severe incomplete ischemia, induced by occlusion of the abdominal aorta caudal to the left renal artery for 40 min, and postischemic reperfusion for 6 h, 1 and 4 days on phospholipid composition and unesterified cholesterol concentration was studied in the lumbosacral (L3-S1) spinal cord separated into the gracile fascicle (Fg), dorsal part without Fg (Dp) and ventral part (Vp). Ischemia decreased the inositol phospholipid (PI) concentration in Dp and Vp and this was recovered during reperfusion. Within 6 h following ischemia, ethanolamine (PE) and serine (PS) phospholipid concentrations decreased in Dp and PS also in Vp. During the long reperfusion intervals the concentrations of the two major phospholipids, PE and choline phospholipid (PC) declined in Fg, Dp and Vp. No changes were observed in sphingomyelin (SM). The concentration of unesterified cholesterol (UC) was lower throughout the reperfusion period in Dp and Vp, while the decrease in Fg was delayed. The molar ratio UC/TPL was reduced starting from 24 h of reperfusion. The pattern of changes, which were delayed in the white matter as compared to Dp and Vp (containing the gray matter) indicated severe damage to the membrane structures in the tissue, developed during reoxygenation, that was related to decreased tissue viability.","PeriodicalId":20547,"journal":{"name":"Physiologia Bohemoslovaca","volume":"39 4","pages":"351-60"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13304303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pulmonary endocrine cells in chronic bronchitis and emphysema. 慢性支气管炎和肺气肿中的肺内分泌细胞。

Physiologia Bohemoslovaca Pub Date : 1990-01-01

M Sissons, J Gosney

引用次数: 0