Proceedings for Annual Meeting of The Japanese Pharmacological Society最新文献_第7页

Involvement of Pgc-1α in the skeletal muscle on glucose uptake via the peripheral sympathetic nervous system in rats 大鼠骨骼肌中Pgc-1α参与外周交感神经系统葡萄糖摄取

Proceedings for Annual Meeting of The Japanese Pharmacological Society Pub Date : 2022-01-01 DOI: 10.1254/jpssuppl.96.0_yia02-6

D. Sato, Nozomi Imaizumi, M. Kusunoki, L. Miyamoto

引用次数: 0

Role of TRPV1 in hyaluronan-induced apoptosis avoidance in MCF-7 cells TRPV1在透明质酸诱导的MCF-7细胞凋亡避免中的作用

Proceedings for Annual Meeting of The Japanese Pharmacological Society Pub Date : 2022-01-01 DOI: 10.1254/jpssuppl.95.0_1-p-084

Yuki Kaguchi, Yuto Hoshino, Yuichi Fukuyama, Takuya Yamaguchi, J. Yamazaki

引用次数: 0

Identification of the therapeutic target for tendinopathy through the combination of real world data analysis and pharmacological experiments 通过实际数据分析和药理实验相结合，确定肌腱病变的治疗靶点

Proceedings for Annual Meeting of The Japanese Pharmacological Society Pub Date : 2022-01-01 DOI: 10.1254/jpssuppl.96.0_yia05-3

Haruka Furuta, Mari Yamada, T. Nagashima, Shuichi Matsuda, K. Nagayasu, H. Shirakawa, S. Kaneko

引用次数: 0

Organoid-based analysis of human kidney development and disease 基于类器官的人类肾脏发育和疾病分析

Proceedings for Annual Meeting of The Japanese Pharmacological Society Pub Date : 2022-01-01 DOI: 10.1254/jpssuppl.95.0_3-sl11

R. Nishinakamura

{"title":"Organoid-based analysis of human kidney development and disease","authors":"R. Nishinakamura","doi":"10.1254/jpssuppl.95.0_3-sl11","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_3-sl11","url":null,"abstract":"Recapitulating the three-dimensional organ structure in vitro is a major challenge for developmental biology and regenerative medicine. The kidney develops by the reciprocal interactions between the nephron progenitor and ureteric bud, and the origins of these two types of precursors are spatially distinct. Based on these developmental findings, we previously established the induction protocols of the nephron progenitors from pluripotent stem cells (PSCs). Induced nephron progenitors robustly formed nephrons: glomeruli and renal tubules. By generating iPS cellderived nephron organoids from a patient with the congenital nephrotic syndrome, we reproduced the glomerular abnormalities that represent the initial phase of this disease. We also established the protocols to induce the ureteric bud from mouse and human PSCs. Mouse organoids reassembled from the differentially induced ureteric bud and nephron progenitors developed the inherent architectures of the embryonic kidney. Humans ureteric bud organoids were applied to autosomal dominant polycystic kidney disease to successfully reproduce cyst formation in vitro. Thus, kidney organoids will serve as useful basis to analyze human kidney development and disease.","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84166149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of tumor releasing factor, cGAMP on gene expression and calcium response in astrocytes 肿瘤释放因子cGAMP对星形胶质细胞基因表达及钙反应的影响

Proceedings for Annual Meeting of The Japanese Pharmacological Society Pub Date : 2022-01-01 DOI: 10.1254/jpssuppl.95.0_1-o-030

Momoko Goto, Toya Okawa, Daiki Shirane, Hiroki Tanaka, H. Akita, A. Hisaka, Hiromi Sato

引用次数: 0

PDGF-BB-stimulated brain pericytes release the mediators related to blood-brain barrier dysfunction. pdgf - bb刺激的脑周细胞释放与血脑屏障功能障碍相关的介质。

Proceedings for Annual Meeting of The Japanese Pharmacological Society Pub Date : 2022-01-01 DOI: 10.1254/jpssuppl.95.0_1-p-014

Seki Haruka, F. Takata, Yuki Egashira, T. Iwao, Miki Yokoya, Miho Yasunaga, Naotaka Aridome, Yuko Fukunaga, S. Dohgu

引用次数: 0

Antimicrobial activities of ginseng saponins isolated from Red Ginseng to non-tuberculous mycobacteria 红参人参皂苷对非结核分枝杆菌的抑菌活性研究

Proceedings for Annual Meeting of The Japanese Pharmacological Society Pub Date : 2022-01-01 DOI: 10.1254/jpssuppl.96.0_2-b-p-177

Noriaki Samukawa, Takehiro Yamaguchi, K. Samukawa, Kentaro Tokudome, T. Homma, S. Matsunaga, S. Tomita

引用次数: 0

The analysis of Period1 gene expression in vivo and in vitro using a micro PMT system at the early stage in diabets 利用微PMT系统分析早期糖尿病患者体内和体外Period1基因的表达

Proceedings for Annual Meeting of The Japanese Pharmacological Society Pub Date : 2022-01-01 DOI: 10.1254/jpssuppl.95.0_3-p-260

Riku Sato, Megumi Kanai, Miho Wakamatsu, Kotomi Kanagaki, Yoshimitsu Goto, Takeshi Yamaguchi, N. Iigima, K. Hamada, Toshiyuki Hamada

引用次数: 0

Development of a fluorescent labeling method for the live-cell superresolution imaging of synaptic molecules. 突触分子活细胞超分辨成像荧光标记方法的发展。

Proceedings for Annual Meeting of The Japanese Pharmacological Society Pub Date : 2022-01-01 DOI: 10.1254/jpssuppl.95.0_1-p-001

Ishikawa Hiroki, S. Namiki, D. Asanuma, K. Hirose

{"title":"Development of a fluorescent labeling method for the live-cell superresolution imaging of synaptic molecules.","authors":"Ishikawa Hiroki, S. Namiki, D. Asanuma, K. Hirose","doi":"10.1254/jpssuppl.95.0_1-p-001","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_1-p-001","url":null,"abstract":"Synaptic transmission is regulated by nanoscale assembly of synaptic molecules. Stimulated emission depletion (STED) microscopy is a modality of superresolution microscopy that allows visualizing such nanoscale molecular distribution. However, photobleaching severely limits time-series imaging by STED microscopy in living neurons. In this study, we aimed to develop a fluorescence labeling method that circumvents the limitation due to photo-bleaching in STED microscopy. Our method is based on a fusion protein tag that reversibly binds a small organic dye and turns on its fluorescence emission. We reasoned that this reversibility in binding enables everlasting imaging because the bleached dye should be continuously displaced by a fresh dye. Accordingly, we prepared expression constructs of the protein tag fused with synaptic molecules, RimBP2, CAST, and Rim1a. When expressed in cultured hippocampal neurons, the synaptic localization of these molecules was visualized under a standard fluorescence microscope. We successfully performed STED imaging of RimBP2 and CAST with the spatial resolution of less than 100 nm at 0.2 Hz for 10 min. Thus, our fluorescence labeling method enables live STED microscopy in neurons which will be useful to unveil the dynamic feature of nanoscale molecular distribution underlying synaptic function. Poster Session","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79935412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CDK8/19 inhibitors induce M2-like macrophage polarization. CDK8/19抑制剂诱导m2样巨噬细胞极化。

Proceedings for Annual Meeting of The Japanese Pharmacological Society Pub Date : 2022-01-01 DOI: 10.1254/jpssuppl.96.0_1-b-p-068

Natsumi Mizuno, S. Shiga, Y. Yanagawa

引用次数: 0