Plant Growth Regulation最新文献

{"title":"Unraveling jasmonate-mediated plant immunity against green peach aphid (Myzus persicae) in peach: an integrated transcriptomic and RNA sequencing approach","authors":"Xianyou Wang, Yi Zeng, Li Li, Rongyao Fan, Yujun Yan, Longlong Guo, Ruijin Zhou","doi":"10.1007/s10725-024-01198-y","DOIUrl":"https://doi.org/10.1007/s10725-024-01198-y","url":null,"abstract":"<p>The green peach aphid (GPA) is considered one of the most destructive pests posing a significant threat to the growth and fruit quality of peach trees (<i>Prunus persica</i>). Long non-coding RNAs (lncRNAs) represent an essential group of endogenous RNAs that play gene regulatory roles in plants. In this study, we identified 1776 lncRNAs from healthy and GPA-infested <i>P. persica</i> tissues, employing high-throughput strand-specific RNA sequencing. Our rigorous analysis of differential gene expression yielded 2871 differentially-expressed genes (DEGs), with 1803 genes exhibiting upregulation and 1068 genes exhibiting downregulation in response to the presence of GPA in peach trees. Our findings reveal the potential of lncRNAs to serve as crucial microRNA (miRNA) targets, thereby exerting a significant influence on miRNA activity. We further predicted two differentially expressed lncRNA–DEG pairs (circ16–miR482a and circ116–miR319a) associated with jasmonic acid (JA) pathway. Notably, endogenous JA levels in peach trees were continuously induced, primarily as a resistance mechanism against GPA infestation. Furthermore, spray application of JA significantly curtailed the GPA population.</p>","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"166 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141942239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of individual and combined water deficit and high temperature on maize seed set: a field study","authors":"Xiwei Liu, Xuhong Chang, Demei Wang, Yanjie Wang, Xinglong Wang, Qingfeng Meng, Pu Wang","doi":"10.1007/s10725-024-01200-7","DOIUrl":"https://doi.org/10.1007/s10725-024-01200-7","url":null,"abstract":"<p>Drought and heat during flowering critically reduce maize seed set. Current understanding of how these conditions affect pollen release and silk development, which are key determinants of seed set, remains inadequate, particularly under combined water deficit (WD) and high temperature (HT) stresses. This study evaluated the effects of drought and heat on seed set in two maize hybrids, Zhengdan 958 and Demeiya 1, derived from temperate and cool-temperate regions, respectively. These hybrids were exposed to conditions of water deficit, high temperature, and combined water deficit and high temperature (WDHT) within semi-automatic rainout shelters in field ponds, enabling precise simulation of environmental stresses. Relative to controls, seed set in Zhengdan 958 decreased by 32% under WD, 30% under HT, and 41% under combined WDHT conditions. In Demeiya 1, seed set reductions were 26% for WD, 25% for HT, and 34% for WDHT. These reductions were linked to notable decreases in silk fresh weight, pollen weight, and pollen activity. Additionally, the ¹³C content in anthers and the dry matter allocated to tassels decreased by 14.5–53.5% and 3.0–21.0%, respectively. Similarly, the ¹³C content in silks and dry matter allocated to ears decreased by 61.0–91.5% and 14.0–40.0%, respectively. Cross-pollination studies indicated that both hybrids exhibited similar sensitivities to WD and HT; however, silk was more vulnerable under WD and pollen more so under HT in Zhengdan 958, while the reverse was true for Demeiya 1. The combined stresses of WDHT had the most severe effects on both silk and pollen in both hybrids. The observed decreases in seed set under stress conditions were primarily due to limited carbohydrate and dry matter accumulation in reproductive tissues, which impacted silk weight, pollen release, and viability. These findings highlight the critical impact of environmental stresses on the reproductive success of maize, emphasizing the need for strategies to enhance resilience to combined abiotic stresses.</p>","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"53 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141882379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dissecting the roles of increased mRNA m6A methylation in autotetraploidization in Stevia rebaudiana","authors":"Juan Liu, Mingpu Tan, Yingying Zhang, Jie Zhao, Huijie Liu, Peng Liu, Wenna Meng, Anping Ding, Zengxu Xiang, Mingjia Chen","doi":"10.1007/s10725-024-01199-x","DOIUrl":"https://doi.org/10.1007/s10725-024-01199-x","url":null,"abstract":"<p>N6-methyladenosine (m⁶A) is the most abundant modification in eukaryotic mRNA. m⁶A functions in embryo development, flowering time regulation, and fruit ripening. Although polyploidization, a significant factor in plant evolution, leads to phenotypic changes, the roles of m⁶A in plant polyploidization remain unclear. Here, we observed increased leaf area, fresh weight, and thickness upon autotetraploidization in stevia (<i>Stevia rebaudiana</i>). To examine phenotypic and molecular changes following polyploidization, we quantified m⁶A abundance in RNA and conducted m⁶A immunoprecipitation sequencing (m⁶A-seq) and transcriptome analysis of autotetraploid and diploid stevia. Polyploidization led to increased m⁶A levels in RNA, especially in mRNA. m⁶A-seq methylome profiling revealed ~ 20,000 transcripts containing m⁶A, primarily in 3′ untranslated regions. Moreover, 2642 differentially modified m⁶A peaks (DMPs) were hypermethylated (hyper-DMPs) post polyploidization, and transcripts with hyper-DMPs were mainly associated with zeatin and flavonoid biosynthesis. Comparative analysis unveiled a possible correlation between m⁶A methylation and mRNA abundance, as confirmed by in vitro mRNA stability assays. The transcripts of many candidate genes involved in auxin, cytokinin, wax, and DNA biosynthesis, the cell cycle, and the cell wall exhibited hypermethylation and higher abundance in autotetraploid stevia. The contents of wax and auxin compounds significantly increased in autotetraploid stevia, suggesting that m⁶A modification helps maintain higher expression of these target genes. Our findings point to an m⁶A-orchestrated regulatory circuit where m⁶A hypermethylates and upregulates DMP-marked transcripts of auxin and wax biosynthesis genes, thereby determining the accumulation of auxin and wax compounds following stevia polyploidization, which contributes to the phenotypic changes following its autotetraploidization.</p>","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"18 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141882374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Constitutive expression of MsPROPEP1 enhances salinity and drought tolerance in alfalfa (Medicago sativa)","authors":"Haoyan Tang, Xinying Guo, Jingwen Shang, Wenqi Li, Jiaqi Fu, Yuguang Song, Wei Dong","doi":"10.1007/s10725-024-01183-5","DOIUrl":"https://doi.org/10.1007/s10725-024-01183-5","url":null,"abstract":"<p>Plant peptide hormones play crucial roles in plant development, defense against pathogens, and tolerance to abiotic stress. However, only a limited number of hormone-like peptides have been confirmed to contribute to salt and drought stress tolerance in plants beyond <i>Arabidopsis</i>. This study focuses on the isolation and characterization of <i>MsPROPEP1</i>, a propeptide precursor gene from the leguminous grass <i>Medicago sativa</i>. The transcription of <i>MsPROPEP1</i> was shown to be inducible by NaCl, polyethylene glycol (PEG), and abscisic acid (ABA). Constitutive expression of <i>MsPROPEP1</i> in alfalfa seedlings alleviated growth restrictions caused by salinity or osmotic stress and increased sensitivity to ABA-induced stomatal closure. Furthermore, application of synthesized MsPep1 peptide enhanced tolerance to stress induced by NaCl and PEG. In <i>MsPROPEP1</i> overexpression transgenic plants, activation of several ABA-dependent stress-responsive genes was observed. These results highlight <i>MsPROPEP1</i> as a potential candidate for genetic manipulation to enhance salinity and drought tolerance in legume species.</p>","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"295 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141870140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foliar application of abscisic acid and glycine betaine induces tolerance to water scarcity in wheat","authors":"Md. Sabibul Haque, Kh Sabbir Hossain, Artho Baroi, Salman Alamery, KOTB A. Attia, Yaser M. Hafez, Md. Tanveer Hussain, Md. Nesar Uddin, Md. Alamgir Hossain","doi":"10.1007/s10725-024-01190-6","DOIUrl":"https://doi.org/10.1007/s10725-024-01190-6","url":null,"abstract":"<p>The global climate model predicts frequent and severe droughts in the future, resulting in limited crop production affecting growth and yield. The extent of drought tolerance in two wheat cultivars (WMRI-1 and BARI GOM-33) was investigated by foliar application of abscisic acid (ABA) and glycine betaine (GB) at the anthesis and grain filling stages. A pot experiment maintaining a completely randomized design with four replications was set in the field laboratory of the Department of Crop Botany, Bangladesh Agricultural University, Mymensingh, Bangladesh. A total of four treatments were followed such as (i) Control (20–22% moisture content), (ii) Drought (8–10% moisture content), (iii) Drought + ABA, and (iv) Drought + GB. The plants at the reproductive stage were sprayed weekly with ABA (20 µM) and GB (50 mM) solutions under drought conditions. Drought stress significantly declined chlorophyll content and photosynthetic rate in comparison to control. However, the foliar application of ABA and GB under drought considerably increased the rate of photosynthesis and pigment contents in the flag leaves compared to the plants grown under individual drought conditions. A considerable increase in lipid peroxidation and H₂O₂ content in flag leaves was recorded in all drought conditions, while these values were reduced in ABA and GB treatments. ABA and GB application significantly enhanced the catalase and peroxidase enzyme activities and total antioxidant capacity, reducing oxidative damage and increasing grain yield. A hierarchical clustering heatmap using the stress tolerance index (STI) showed that Drought + ABA and Drought + GB secured higher STI scores, suggesting a greater drought tolerance in both cultivars compared to individual drought treatments. In conclusion, foliar spraying of ABA and GB enhanced drought tolerance in both wheat cultivars by altering physiology and antioxidative defense, suggesting a declined state of oxidative damage with increased yield.</p>","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"107 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141870138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive expression analysis of ERF transcription factors during chilling acclimation in Saintpaulia","authors":"Daichi Kurata, Kento Fukutomi, Kanae Kubo, Kenta Shirasawa, Hideki Hirakawa, Munetaka Hosokawa","doi":"10.1007/s10725-024-01181-7","DOIUrl":"https://doi.org/10.1007/s10725-024-01181-7","url":null,"abstract":"<p>Saintpaulia (<i>Saintpaulia ionantha</i>), a popular indoor ornamental potted plant, is native to the highlands of Kenya and Tanzania where temperatures rarely fall below 4 °C. Chilling injury during cultivation and transportation is a major commercial problem in Saintpaulia. In this study, we investigated chilling acclimation in Saintpaulia ‘Kilauea’. Plants grown at 20 °C (14 h light/10 h dark) displayed rapid and severe chilling injury after 24-h exposure to 4 °C. However, chilling injury at 4 °C could be dramatically reduced by pre-treating the plants at 10 °C but not at 6 °C. From whole genome analysis, 161 ethylene-responsive factors (ERFs) were identified and classified into 12 clades according to existing reports. Among these ERFs, 43, 8, and 4 ERFs were upregulated at 12, 24, and 48 h after 10 °C treatment, respectively. Most of these ERFs had GCC box and/or DRE/CRT core motifs-like sequences in their upstream regions. Finally, we compared the expression of ERFs between the treatments for 24 h at 10 °C, an effective temperature for chilling acclimation, and 6 °C, an ineffective temperature. The results showed that the expression of all six ERFs we investigated was increased by the 10 °C treatment, but not or only barely increased by the 6 °C treatment. This study suggests that Saintpaulia, a subtropical plant, can acclimate to low temperatures and that ERF upregulation is involved in chilling acclimation.</p>","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"51 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141870139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated physio-biochemical and transcriptomic analysis reveals mechanism underlying salt tolerance in walnut","authors":"Xu Zheng, Ruining Nie, Ao Li, Chengxu Wu, Xinying Ji, Jiali Tang, Junpei Zhang","doi":"10.1007/s10725-024-01193-3","DOIUrl":"https://doi.org/10.1007/s10725-024-01193-3","url":null,"abstract":"<p>Walnut, an essential oilseed tree, is widely cultivated in regions such as Xinjiang, China. However, extensive saline-alkali land has significantly reduced the walnut field. Consequently, breeding salt-tolerant walnut rootstocks is crucial to development of industry. In this study, two walnut varieties (Cd and Xw) were subjected to NaCl salinity (0, 50, 100, 150 mM) to investigate physiological and molecular mechanisms of salt stress resistance. Our results revealed that salinity stress reduced growth parameters (shoot biomass, root biomass, relative ground and height growth) and photosynthetic pigments (Pn, Tr, and Cond) in both Cd and Xw compared to control plants. Additionally,as stress duration increased, proline content and antioxidant enzyme activity decreased. Transcriptomic analysis identified 9077 differentially expressed genes (DEGs), with Gene Ontology(GO) analyses indicating their involvement in hormone signaling, photosynthesis, light harvesting, and photosystems (I and II). WGCNA analysis identified hub genes highly associated with salt response, focusing on the light-harvesting chlorophyll protein complex <i>(JrLHCb5</i>, <i>JrLHCa</i>), MAPK signaling pathway (<i>JrSAPK2</i>), and hormone signaling processes (<i>JrPYL4</i>, <i>JrSCL13</i>). This study provides scientific evidence for further exploration of physiological and molecular mechanisms of salt tolerance in walnuts.</p>","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"13 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141870089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics and related genes analysis revealed the distinct mechanism of drought resistance in novel buckwheat and cultivated species","authors":"Xiao Zhang, Miao Yang, Zhang Liu, Yi Huang, Lei Zhang, Fan Yang, Junqing Gong, Dongao Huo","doi":"10.1007/s10725-024-01189-z","DOIUrl":"https://doi.org/10.1007/s10725-024-01189-z","url":null,"abstract":"<p>Buckwheat (<i>Fagopyrum tataricum</i>), a crop with both food and medicinal applications, holds significant economic value particularly in regions with arid or barren land. In order to explore the physiological and metabolic mechanism of drought stress resistance in different varieties of buckwheat, we conducted analyses on morphophysiological indicators and metabolomes of two distinct drought-tolerant buckwheat varieties exposed to drought treatments of different durations. The results revealed that drought stress exacerbated cell membrane damage, reduced chlorophyll content, and impaired antioxidant enzyme activity in both buckwheat varieties. However, the novel species DK19 exhibited greater resistance to drought compared to cultivated buckwheat K33. Metabolomics approaches were employed to examine metabolite changes in DK19 and K33 during two critical periods (day 7 and day 11) of drought stress. As a result, 15 significantly altered metabolites were identified in different materials within the network of amino acid biosynthesis pathways. Furthermore, 16 significantly enriched metabolites in the phenylpropanoid biosynthesis pathway as well as 17 significantly enriched metabolites in flavonoids biosynthesis pathway were detected. Additionally, based on metabolite changes, we determined differential expression levels of six genes related to amino acid metabolism networks and 12 key genes highly associated with secondary metabolism. Moreover, we observed distinct accumulation patterns of secondary metabolites phenylpropanoids (including hydroxycinnamates and flavonoids) in the two materials, which may contribute to the improved drought resistance of the novel tartary buckwheat cultivars. These findings provide valuable clues for the breeding of perennial buckwheat and solid foundations for genetically improving the drought resistance of buckwheat in the future.</p>","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"63 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141784088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimating the role of maize Y-EPSPS gene in glyphosate resistance in Arabidopsis transgenic lines","authors":"Fan-Hao Wang, Chen Zhang, Chun-Lai Wang, Xiao-Tong Wei, Si-Yan Liu, Shu-Yan Guan, Yi-Yong Ma","doi":"10.1007/s10725-024-01188-0","DOIUrl":"https://doi.org/10.1007/s10725-024-01188-0","url":null,"abstract":"<p>The herbicide glyphosate inhibits the key enzyme 5-enolpyruvate shikimate-3-phosphate synthase (EPSPS) in the aromatic amino acid synthesis pathway of plants. This study aims to explore the <i>Y-EPSPS</i> gene derived from maize through codon optimization and validate its glyphosate resistance in Arabidopsis Thaliana. Germination rates of seeds under different glyphosate concentrations revealed that seeds overexpressing the <i>Y-EPSPS</i> gene exhibited higher germination rates compared to wild-type seeds. DAB and NBT staining methods were used to measure ROS levels in Arabidopsis plants under 0.8 mM glyphosate stress, showing that plants overexpressing <i>Y-EPSPS</i> had lower ROS levels compared to wild-type plants. Soluble sugar and malondialdehyde (MDA) content were higher in <i>Y-EPSPS</i> overexpressing plants, whereas MDA content was lower, indicating a potential stress response to glyphosate. Chlorophyll content and FV/FW ratio were higher in plants overexpressing <i>Y-EPSPS</i> compared to wild-type plants, suggesting reduced susceptibility to glyphosate. Enzyme activity and gene expression analysis further demonstrated significant increases in POD, SOD, and CAT enzyme activities in <i>Y-EPSPS</i> overexpressing plants compared to wild-type, while SD enzyme activity decreased significantly. Expression levels of ROS detoxification-related genes (<i>AtCAT3</i> and <i>AtSOD1</i>) and stress defense-related genes (<i>AtLTP3</i>, <i>AtSOS1</i>, and <i>DQSD</i>) were also elevated to varying degrees in <i>Y-EPSPS</i> overexpressing plants compared to wild-type plants. These results indicate that the optimized <i>Y-EPSPS</i> gene confers certain resistance to glyphosate.</p>","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"42 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141784089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of three DREB-like genes encoding dehydration-responsive element binding proteins in chicory (Cichorium intybus)","authors":"Ji Wang, Long Zhao, Mengjia Zhou, Ling Yang, Yu Cheng, Ebru Toksoy Öner, Huijuan Zhang, Jingjing Xu, Qingsong Zheng, Mingxiang Liang","doi":"10.1007/s10725-024-01167-5","DOIUrl":"https://doi.org/10.1007/s10725-024-01167-5","url":null,"abstract":"<p>Three novel DREB (Dehydration-Responsive Element Binding protein) genes, designated as <i>CiDREB2A</i>, <i>CiDREB5A</i> and <i>CiDREB6A</i>, were individually isolated from chicory (<i>Cichorium intybus</i> L). Based on phylogenetic tree analysis, all three genes possessed a conserved AP2/EREBP domain and were subsequently categorized into the A2, A5, and A6 subgroups within the DREB subfamily, respectively. All of them contained a conserved AP2/EREBP domain and were classified into the A2, A5 and A6 subgroup of the DREB subfamily based on phylogenetic tree analysis, respectively. Quantitative real-time PCR analysis indicated that these genes were all induced by abiotic stresses. A subcellular localization assay revealed that these CiDREBs were localized in the nucleus. In addition, these three CiDREB proteins were also identified to bind the DRE motif of <i>RD19A</i> as shown by yeast one-hybrid experiments. Overexpression of <i>CiDREB2A</i> was found to promote sensitivity to high temperature at the post-germination stages in Arabidopsis while expression of <i>heat shock proteins</i> were decreased in the overexpressing plants compared to that of the control group under high temperature treatment. These results suggest that these CiDREBs may play an important role in regulation of stress-responsive signaling in chicory. </p>","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"16 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141572807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}