Plant Growth Regulation最新文献

{"title":"OsPCK1 regulates photosynthate transport from source to sink and affects grain filling in rice","authors":"Chengke Li, Xin Chen, Xiuqin Qiao, Yue Xu, Hui Xu, Lihong Zong, Zhiping Gao","doi":"10.1007/s10725-026-01493-w","DOIUrl":"https://doi.org/10.1007/s10725-026-01493-w","url":null,"abstract":"Ensuring global food security hinges on increasing rice ( Oryza sativa L.) yield, and grain-filling is a primary determinant of key yield components such as grain size and weight. Phospho enol pyruvate carboxykinase (PEPCK), encoded by the PCK gene (LOC_Os03g15050), has been identified in C 3 plants including rice, however, its biological roles and regulatory networks remain poorly understood in the absence of a complete C 4 photosynthetic apparatus. As a pivotal enzyme in gluconeogenesis, PEPCK may be intimately involved in carbon partitioning and the mobilization of photosynthetic products during grain filling. To investigate the functional mechanism of OsPCK1 (a transcript of rice PCK) Oryza sativa L. ssp. japonica Nipponbare (wild type, WT), the OsPCK1 knockout mutant ( ospck1 ), and overexpression lines ( OX10 , OX15 ) were employed as experimental materials. Through integrated phenotypic characterization, physiological measurements, and molecular analyses, we demonstrated that OsPCK1 positively regulated the expression of genes controlling panicle architecture and grain morphology. Additionally, OsPCK1 may play a role in facilitating the translocation of photoassimilates to reproductive organs, by upregulating key genes associated with sugar transport ( OsSUTs , OsSWEETs ) and starch biosynthesis ( OsAGPS1 , OsAGPL1 ), thereby improving yield-related traits. Conversely, the loss of OsPCK1 function resulted in significant downregulation of these genes, leading to compromised panicle fertility with reduced grain number and decreased 1000-grain weight. Our results suggest that OsPCK1 may regulate carbon allocation by concurrently influencing photosynthetic carbon assimilation and starch accumulation in grains, thereby contributing to sugar transport and starch biosynthesis during grain filling. These findings offer new insights into PEPCK function in C 3 plants and may provide a reference for future strategies aimed at increasing rice yield by optimizing carbon allocation.","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148672120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-harvest methyl jasmonate application enhances essential oil yield and citral content in Camphora officinarum Nees ex Wall","authors":"Qingyan Ling, Beihong Zhang, Zufei Xiao, Yao Shen, Jun Jiang, Zhiwei Xu, Qingqing Liu, Changlong Xiao, Rong Zeng, Yuanqiu Liu, Zhinong Jin","doi":"10.1007/s10725-025-01367-7","DOIUrl":"https://doi.org/10.1007/s10725-025-01367-7","url":null,"abstract":"Citral-type Camphora officinarum (C. officinarum) exhibits high economic value due to its branch and leaf essential oil enriched with citral, yet the low natural concentration of this compound necessitates urgent strategies to enhance yield and quality. Methyl jasmonate (MeJA), a recognized safe elicitor, stimulates aromatic compound accumulation in plants and promotes monoterpene synthesis in C. officinarum suspension cells. However, it remained unclear whether foliar MeJA application enhances monoterpene biosynthesis in C. officinarum. This study evaluated the effects of near-harvest foliar MeJA application at gradient concentrations 0 μmol/L (control, M0), 100 μmol/L (M100), 250 μmol/L (M250), and 500 μmol/L (M500) on growth, oil gland morphology, and essential oil composition in citral-type C. officinarum. The results demonstrated that: M100 treatment significantly increased plant height, branch biomass, leaf biomass, and total biomass compared to the control M0. M250 treatment induced structural modifications in oil cells, enlarging their diameter and increasing density, resulting in an 11.83% increase in essential oil yield per unit fresh leaf weight. Both M100 and M250 treatments enhanced citral content by 3.6% and 3.31%, respectively, while total essential oil production surged by 12.31% (M100) and 15.46% (M250). Principal component analysis (PCA) collectively demonstrated that 100–250 μmol/L MeJA (M100/M250) represented the optimal induction concentration for enhancing essential oil yield and promoting citral synthesis, whereas 500 μmol/L (M500) induced detrimental osmotic stress. These findings demonstrate that MeJA serves as a safe agricultural regulation approach for enhancing essential oil yield and citral content, offering valuable insights for other aromatic plant species.","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"105 6","pages":"1991-2006"},"PeriodicalIF":0.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10725-025-01367-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotype-dependent enhancement of tomato transformation and regeneration by GRF4 and GIF1","authors":"Yaping Tang, Xinyan Shen, Xuan Deng, Guoru Zhang, Lei Song, Bo Liu, Shengbao Yang, Yongen Lu, Bo Ouyang","doi":"10.1007/s10725-025-01381-9","DOIUrl":"https://doi.org/10.1007/s10725-025-01381-9","url":null,"abstract":"Agrobacterium-mediated genetic transformation of plants, including tomato, faces challenges related to genotype variability and regeneration rates. Research suggests that growth-regulating factors (GRFs) and their interacting factors (GIFs) are key to enhancing transformation and regeneration in various plant species. However, their specific effects on tomato remain underexplored. In this study, we identified and analyzed 11 SlGRF homologous genes in tomato and validated the interaction between SlGRF4 and SlGIF1. In addition, we demonstrated the effectiveness of the RUBY visual marker system in tomato transgenic research. Developmental regulators significantly influenced callus transformation and shoot regeneration rates across three tomato genotypes, with the SlGRF4-GIF1 combination providing the most consistent enhancement in both processes. These findings hold promise for tomato improvement via genome editing.","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"105 5","pages":"1789-1801"},"PeriodicalIF":0.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10725-025-01381-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147885323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of terminal heat stress on stay-green and senescence process could explain genetic variation in grain yield and nutritional profile in wheat (Triticum aestivum L.)","authors":"M. V. Lekhana, Rajesh Kumar Meena, T. V. Anirudh, Mainak Barman, Abhishek Kumar, M. S. Sai Reddy, Amit Sharma, Rajeev N. Bahuguna, Satish Kumar Singh","doi":"10.1007/s10725-025-01345-z","DOIUrl":"https://doi.org/10.1007/s10725-025-01345-z","url":null,"abstract":"Wheat is a staple food crop for the majority of the world’s population. Heat stress affects reproductive development, seed-set and seed weight, eventually reducing yield and total biomass in wheat. Stay-green contributes to seed weight by allowing more assimilate partitioning to grain. Conversely, senescence is essential for the recycling of nutrients to developing grain. We have studied a set of 26 high-yielding wheat genotypes to study the effect of terminal heat stress on the stay-green and senescence process to explain genetic variation and trade-off associated between grain yield and grain nutrients under field conditions. Our results revealed that heat stress accelerated the senescence process and significantly reduced average grain filling duration by 8 days. The negative effect of heat stress on spikelet fertility and seed weight resulted in reduced grain yield (25%). On the contrary, a positive influence of heat was observed on grain protein (6–21%), grain minerals, and reduced C:N ratio. Interestingly, genotype (HTWYT42) with a longer stay-green period, showed higher yield under heat stress, whereas genotype (HTWYT43) showed early onset but a slower pace of senescence accumulating more grain protein, minerals and lower C:N ratio under heat stress. This study demonstrates a strong trade-off between grain yield and nutrition. However, maintaining an optimum balance between stay-green and a slower rate of senescence could be crucial to minimizing this trade-off, and achieving both higher yield and improve grain nutrition. Furthermore, understanding the mechanisms underlying slower senescence in source tissues could be essential for developing wheat genotypes with enhanced grain nutrient content.","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"105 5","pages":"1545-1557"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10725-025-01345-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GW4 is regulated by GS2 to control grain width in rice","authors":"Kaixiong Wu, Yueying Wang, Xuemei Deng, Jialong Liu, Yi Wen, Junge Wang, Bingze Chai, Yue Wu, Peng Hu, Hao Wu, Lixin Zhu, Huisen Wang, Shuxian Gan, Guangheng Zhang, Li Zhu, Deyong Ren, Qiang Zhang, Yuchun Rao, Bin Zhang, Jiang Hu","doi":"10.1007/s10725-025-01303-9","DOIUrl":"https://doi.org/10.1007/s10725-025-01303-9","url":null,"abstract":"Grain width is one of the important factors determining grain yield and appearance quality. However, the genetic and molecular mechanisms controlling grain width remain be largely unexplored. In this study, we characterized a grain width mutant gw4 (grain width on chromosome 4), which showed reduced grain width but did not affect grain length. Sequence analysis revealed that a single base substitution (G to A) of LOC_Os04g01590 in gw4 resulted in the conversion of original encoded glycine to aspartate. The gene encodes an arginase that is localized on the mitochondrial and is highly expressed in panicles. RNA-seq and expression analysis showed that gw4 can control glume width by regulating the expression of cell cycle and cell expansion genes. Additionally, we also found that GS2 acts upstream of GW4 and regulates its expression by directly binding to GW4 promoter. In conclusion, we revealed that GW4 plays an important role in maintaining rice grain development. These results provide new insight into the genetic mechanism of arginase in the control of grain width and provide a potential target for molecular breeding of grain size.","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"105 4","pages":"1181-1193"},"PeriodicalIF":0.0,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10725-025-01303-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147913006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential phytohormone related senescence regulation in main and lateral roots of longan (Dimocarpus longan Lour.)","authors":"Lin Liu, Liqin Liu, S. Y. Shi, Bo Shu","doi":"10.1007/s10725-025-01327-1","DOIUrl":"https://doi.org/10.1007/s10725-025-01327-1","url":null,"abstract":"Root senescence (RS) is a common phenomenon in longans; however, its regulation is poorly understood. In this study, the main root was divided into three segments from tip to crown, i.e., distal (MDR), middle (MMR) and proximal (MPR) parts, and lateral roots emerging from the corresponding segments of the main root, i.e., lateral roots from the distal (LDR), middle (LMR) and proximal (LPR) parts, were used for analysis of morphological characteristics, physiological root activity, transcriptome, proteome, and phytohormone quantification, in order to understand the regulation of RS in longan seedlings. The root hair and epidermis of the proximal parts of the main and lateral roots exhibited senescence, and activity decreased from the distal to the proximal parts, suggesting that both the main and lateral roots underwent senescence. The transcriptome profile showed that 2692 and 1406 differentially expressed genes were involved in the RS of the main and lateral roots, respectively, and the proteome data exhibited 222 and 124 differentially accumulated proteins involved in the RS of the main and lateral roots, respectively, based on the MPR vs. MMR and LPR vs. LMR groups. Based on the correlation analysis of DAPs and DEGs, the KEGG pathways of carotenoid biosynthesis (abscisic acid) and steroid biosynthesis (brassinosteroid) were found to be significantly different in the main roots, and two KEGG pathways related to plant hormones, phenylalanine metabolism (salicylic acid) and cysteine and methionine metabolism (ethylene), were significant in the lateral roots. Furthermore, the abscisic acid concentration in MMR was 9.47% lower than that in MPR and was negatively related to the root activity of the main root. The concentration of 1-aminocyclopropane-1-carboxylic acid increased 1.59 times from LDR to LPR and was negatively related to the activity of the lateral root. Thus, the results of this study indicate that different phytohormones are related to senescence regulation in the main and lateral roots of longan seedlings. In addition, transcriptome and proteome data provide basic information related to RS in longan seedlings.","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"105 4","pages":"1139-1156"},"PeriodicalIF":0.0,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10725-025-01327-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147885454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin improves aluminum resistance in rice (Oryza Sativa) via a nitric oxide dependent pathway","authors":"Chun Yan Tu, Yong Qiang Gao, Lu Zheng, Jun Wei, Ren Fang Shen, Xiao Fang Zhu","doi":"10.1007/s10725-025-01302-w","DOIUrl":"https://doi.org/10.1007/s10725-025-01302-w","url":null,"abstract":"Aluminum (Al) toxicity represents a significant constraint on crop growth in acidic soils. Al stress can stimulate the biosynthesis of melatonin (MT) in rice (Oryza sativa L.). The exogenous application of 20 μM MT markedly reduces Al accumulation in root tips and effectively mitigates Al stress. By enhancing the levels of root hemicellulose and down-regulating the expression of two genes sensitive to aluminum rhizotoxicity (OsSTAR1 and OsSTAR2), which function to obscure Al binding sites within the cell wall, MT significantly improves the fixation of Al by the cell wall. Furthermore, MT diminishes cytoplasmic entry of Al by reducing expression levels of nramp aluminum transporter 1 gene (OsNRAT1), while inducing expression of an aluminum-sensitive gene (OsALS1), thereby promoting vacuolar compartmentation to lower cytoplasmic Al concentration. Additionally, MT substantially decreases Nitric oxide (NO) accumulation induced by Al in roots. The alleviation effect exerted by MT on Al toxicity can be replicated through administration of the NO scavenger carboxyl-PTIO (c-PTIO). Moreover, the Al-sensitive phenotype exhibited in the osgsnor mutant, coupled with the absence of mitigation by MT, further substantiates that MT alleviates Al toxicity in rice primarily via regulation of NO content, potentially relying on NO signal transduction pathways. In summary, MT plays a pivotal role in modulating rice resistance to Al toxicity and stimulates multiple pathways to enhance tolerance against this abiotic stressor. This study provides new insights into understanding mechanisms underlying rice resistance to abiotic stresses.","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"105 3","pages":"707-723"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10725-025-01302-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147909787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and metabolomic unraveling of nitrogen use efficiency in sorghum: the quest for molecular adaptations to low-nitrogen stress","authors":"Wendong Gu, Yihao Feng, Chang Liu, Xiaolong Shi, Lei Han, Chunjuan Liu, Yufei Zhou","doi":"10.1007/s10725-025-01296-5","DOIUrl":"https://doi.org/10.1007/s10725-025-01296-5","url":null,"abstract":"Sorghum (Sorghum bicolor L. Moench), renowned for its resilience in marginal environments, frequently encounters productivity limitations due to insufficient nitrogen (N) availability. This investigation employed transcriptomic and metabolomic approaches to elucidate the mechanisms underlying nitrogen use efficiency (NUE) in two sorghum genotypes, 398B and CS3541, exhibiting contrasting NUE responses to low-N stress. N deficiency significantly diminished plant height, root dry weight, and leaf dry weight in both genotypes, while markedly increasing root length. Notably, 398B demonstrated a pronounced increase in root length and exhibited higher nitrogen uptake efficiency (NUpE) and utilization efficiency (NUtE) compared to CS3541. Transcriptomic analyses revealed that starch and sucrose metabolism, as well as phenylpropanoid biosynthesis pathways, were significantly enriched in both genotypes, indicative of enhanced carbon (C) and N metabolism. Notably, two low-affinity nitrate transporter genes (Sobic.001G302800.v3.2, NRT1/PTR6.3 and Sobic.004G193000.v3.2, NRT1/PTR6.4) were upregulated in 398B roots under N deficiency, suggesting a crucial role in facilitating N uptake. Metabolomic profiling of sorghum leaves under low-N conditions highlighted an accumulation of phenolic acids, flavonoids, flavanols, and saccharides in 398B, suggesting improved N utilization and assimilation. Overall, integrated transcriptome and metabolome analyses identified key genes and metabolites associated with NUE. Our findings provide novel insights into the genetic and molecular basis of NUE in sorghum, particularly emphasizing the role of root structural adaptations and the regulation of specific transporter genes in enhancing NUpE. Additionally, our study underscored the importance of metabolic adjustments in leaves for improving NUtE. These results contribute to the development of sorghum genotypes with enhanced NUE, offering the potential for sustainable agricultural practices in low-N environments.","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"105 3","pages":"687-706"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10725-025-01296-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and physiological analysis reveals the accumulation mechanism of anthocyanin and apigenin in purple celery (Apium graveolens L.)","authors":"Miao Sun, Peng Zhao, Tao Yang, Xuan-Huan Qiao, Jiaming Luo, Jun-Hao Zhao, Fangfang Liu, Xiang Li, Ai‐Sheng Xiong","doi":"10.1007/s10725-024-01261-8","DOIUrl":"https://doi.org/10.1007/s10725-024-01261-8","url":null,"abstract":"","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"105 1","pages":"153-165"},"PeriodicalIF":0.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10725-024-01261-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147880377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin in redox network and hormonal balance in mungbean seedlings due to fungicide-induced seed stress","authors":"Manuela Giraldo-Acosta, Antonio Caño, Josefa Hernández‐Ruíz, Marino B. Arnao","doi":"10.1007/s10725-024-01256-5","DOIUrl":"https://doi.org/10.1007/s10725-024-01256-5","url":null,"abstract":"","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"105 1","pages":"55-70"},"PeriodicalIF":0.0,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10725-024-01256-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}