GW4 is regulated by GS2 to control grain width in rice

IF 4 3区生物学 Q1 PLANT SCIENCES

Plant Growth Regulation Pub Date : 2025-05-08 DOI:10.1007/s10725-025-01303-9

Kaixiong Wu, Yueying Wang, Xuemei Deng, Jialong Liu, Yi Wen, Junge Wang, Bingze Chai, Yue Wu, Peng Hu, Hao Wu, Lixin Zhu, Huisen Wang, Shuxian Gan, Guangheng Zhang, Li Zhu, Deyong Ren, Qiang Zhang, Yuchun Rao, Bin Zhang, Jiang Hu

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引用次数: 0

Abstract

Grain width is one of the important factors determining grain yield and appearance quality. However, the genetic and molecular mechanisms controlling grain width remain be largely unexplored. In this study, we characterized a grain width mutant gw4 (grain width on chromosome 4), which showed reduced grain width but did not affect grain length. Sequence analysis revealed that a single base substitution (G to A) of LOC_Os04g01590 in gw4 resulted in the conversion of original encoded glycine to aspartate. The gene encodes an arginase that is localized on the mitochondrial and is highly expressed in panicles. RNA-seq and expression analysis showed that gw4 can control glume width by regulating the expression of cell cycle and cell expansion genes. Additionally, we also found that GS2 acts upstream of GW4 and regulates its expression by directly binding to GW4 promoter. In conclusion, we revealed that GW4 plays an important role in maintaining rice grain development. These results provide new insight into the genetic mechanism of arginase in the control of grain width and provide a potential target for molecular breeding of grain size.

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GW4受GS2调控，控制水稻粒宽

籽粒宽度是决定籽粒产量和外观品质的重要因素之一。然而，控制晶粒宽度的遗传和分子机制在很大程度上仍未被探索。在本研究中，我们鉴定了一个粒宽突变体gw4（4号染色体上的粒宽），该突变体的粒宽减小，但对粒长没有影响。序列分析表明，gw4中LOC_Os04g01590的单碱基置换（G到a）导致原编码的甘氨酸转化为天冬氨酸。该基因编码一种精氨酸酶，这种精氨酸酶位于线粒体上，并在穗中高度表达。RNA-seq和表达分析表明，gw4可以通过调控细胞周期和细胞扩增基因的表达来调控颖片宽度。此外，我们还发现GS2作用于GW4的上游，并通过直接结合GW4启动子调控其表达。综上所述，我们发现GW4在维持水稻籽粒发育中起着重要作用。这些结果为精氨酸酶调控籽粒宽度的遗传机制提供了新的认识，并为籽粒大小的分子育种提供了潜在的靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Growth Regulation 生物-植物科学

CiteScore

6.90

自引率

9.50%

发文量

139

审稿时长

4.5 months

期刊介绍： Plant Growth Regulation is an international journal publishing original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research using hormonal, physiological, environmental, genetical, biophysical, developmental or molecular approaches to the study of plant growth regulation. Emphasis is placed on papers presenting the results of original research. Occasional reviews on important topics will also be welcome. All contributions must be in English.