Polish journal of veterinary sciences最新文献_第8页

Comparative analysis of CpG islands in two genotypes of African swine fever virus. 两种非洲猪瘟病毒基因型CpG岛的比较分析。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2022-09-01 DOI: 10.24425/pjvs.2022.142030

Y-Y Yu, M-S X, Q Liu

{"title":"Comparative analysis of CpG islands in two genotypes of African swine fever virus.","authors":"Y-Y Yu, M-S X, Q Liu","doi":"10.24425/pjvs.2022.142030","DOIUrl":"https://doi.org/10.24425/pjvs.2022.142030","url":null,"abstract":"African swine fever (ASF) is an acute, hemorrhagic, and devastating viral infectious disease that causes important economic losses to the swine industry. Currently, there are no effective vaccines or drugs available. Epigenetic mechanisms, especially cytosine methylation of cytosine- -phosphate-guanine (CpG) islands, have a significant impact on the life cycle of several viruses. Hence, drugs targeting DNA methylation may potentially be used for the treatment of ASF. Here, we selected the inner core, core shell, inner membrane, capsid, and external envelope membrane, to analyze the characteristics of CpG islands in the ASF virus (ASFV) genomes. Furthermore, we analyzed the promoters and CpG islands in the upstream regions of these genes. Results showed that the CpG islands of seven genes were conserved in the genomes of two genotype of ASFV strains, whereas the CpG islands of other genes were relatively conserved (ASFV strains differed mainly in the quantity of CpG islands). The different distribution of CpG islands in the genomes of different ASFV strains may affect their methylation status, which may in turn affect the regulation of viral gene expression, leading to different clinical outcomes. In addition, the predicted promoter regions based on the upstream sequences of most genes overlapped with CpG island positions. Methylation of the binding sites of the promoter regions inhibits the binding of the transcription factors to the promoters, thus inhibiting the activation of the promoters and limiting the synthesis of viral proteins. The results of this study provide a basis for exploring new antiviral therapeutic strategies from an epigenetic perspective.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33482691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular detection and therapeutic study of Trypanosoma brucei evansi from naturally infected horses in Punjab, Pakistan. 巴基斯坦旁遮普自然感染马的布氏伊文氏锥虫分子检测及治疗研究。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2022-09-01 DOI: 10.24425/pjvs.2022.142027

J Zahoor, M Kashif, A Nasir, M Bakhsh, M F Qamar, A Sikandar, A Rehman

{"title":"Molecular detection and therapeutic study of Trypanosoma brucei evansi from naturally infected horses in Punjab, Pakistan.","authors":"J Zahoor, M Kashif, A Nasir, M Bakhsh, M F Qamar, A Sikandar, A Rehman","doi":"10.24425/pjvs.2022.142027","DOIUrl":"https://doi.org/10.24425/pjvs.2022.142027","url":null,"abstract":"Trypanosomiasis is one of the severe pathogenic infections, caused by several Trypanosoma species, affecting both animals and humans, causing substantial economic losses and severe illness. The objective of this study was to determine the molecular diagnosis and the risk factors associated with trypanosomiasis in District Jhang, Punjab, Pakistan. For this purpose, blood samples were randomly collected from 200 horses. A predesigned questionnaire was used to collect data on risk factors before the sample collection. The microscopy examination through Giemsa staining, formol gel test and PCR techniques were used to find the prevalence. The prevalence was recorded as 22.5% with microscopy examination, 21% through formol gel test and 15.5% with PCR based results. Analysis of risk factors associated with Trypanosoma brucei evansi occurrence was carried out using Chi-square test. It showed the prevalence of Trypanosoma brucei evansi was significantly (p⟨0.05) associated with sex, age, rearing purpose and body condition whereas non-significantly (p⟩0.05) with insects control practices. This study supports the idea that PCR is a sensitive, robust and more reliable technique to diagnose trypanosomiasis. It was concluded that Trypanosoma brucei evansi is widely prevalent in Jhang (Pakistan), highlighting a dire need to develop control strategies and education programmes to control this disease in developing countries.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33481422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on the effect of an androgenic hormone product on the functional status of common sebaceous glands in dogs. 雄激素产品对犬普通皮脂腺功能状态影响的研究。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2022-09-01 DOI: 10.24425/pjvs.2022.142023

G Gâjâilă, M Ghiță, G Zagrai, D C Cotor, A M Ionescu, A M Zagrai Măierean, A Damian, G Cotor

{"title":"Research on the effect of an androgenic hormone product on the functional status of common sebaceous glands in dogs.","authors":"G Gâjâilă, M Ghiță, G Zagrai, D C Cotor, A M Ionescu, A M Zagrai Măierean, A Damian, G Cotor","doi":"10.24425/pjvs.2022.142023","DOIUrl":"https://doi.org/10.24425/pjvs.2022.142023","url":null,"abstract":"Nowadays, a high incidence of cutaneous tumors is observed in domestic carnivorous in pet clinic, the sebaceous glands being the starting point for the development of these tumors. The hormonal imbalances are considered to be the most common etiology for these tumors, so the current research is based on the effects of an androgenic like hormonal drug on the functionality of the sebaceous glands in dogs. For this purpose, 32 dogs were distributed in 4 groups: control group 1 (8 dogs - females), control group 2 (8 dogs - males), experimental group 1 (8 dogs - females) and experimental group 2 (8 dogs - males). The investigation targeted the pH of skin and the rate of sebum's secretion. The animals from the experimental groups were treated with Anabolin forte for 2 days at a dose of 1 mg/kg body weight/day, intramuscular. The obtained results revealed that administration of Anabolin forte in males induced a significantly distinct intensification (with 10.66%) of sebum's secretion and a significant decrease of pH of the skin (with 17.1%) compared to the animals from the control group. The administration of Anabolin forte in females induced a significantly distinct intensification (with 17.47%) of sebum's secretion and a significant decrease of the pH of the skin (with 14.32%) compared to the animals from the control group.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33482737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of dexamethasone and minocycline alone and combined with N-acetylcysteine and vitamin E on serum matrix metalloproteinase-9 and coenzyme Q10 levels in aflatoxin B1 administered rats. 地塞米松和米诺环素单用及联用n -乙酰半胱氨酸和维生素E对黄曲霉毒素B1大鼠血清基质金属蛋白酶-9和辅酶Q10水平的影响

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2022-09-01 DOI: 10.24425/pjvs.2022.142026

B Tras, H Eser Faki, Z Ozdemir Kutahya, E Bahcivan, B Dik, K Uney

{"title":"The effects of dexamethasone and minocycline alone and combined with N-acetylcysteine and vitamin E on serum matrix metalloproteinase-9 and coenzyme Q10 levels in aflatoxin B1 administered rats.","authors":"B Tras, H Eser Faki, Z Ozdemir Kutahya, E Bahcivan, B Dik, K Uney","doi":"10.24425/pjvs.2022.142026","DOIUrl":"https://doi.org/10.24425/pjvs.2022.142026","url":null,"abstract":"This study aimed to determine the effects of dexamethasone and minocycline alone and combined treatment with N-acetylcysteine (NAC) and vitamin E on serum coenzyme Q10 (CoQ10) and matrix metalloproteinase-9 (MMP-9) levels in rats administered aflatoxin B1 (AFB1). The study was carried out on 66 male Wistar rats. Following the intraperitoneal (IP) administration of AFB1 at dose of 2 mg/kg, minocycline (45 and 90 mg/kg, IP) and dexamethasone (5 and 20 mg/kg, IP) were administered alone and combined with NAC (200 mg/kg, IP) and vitamin E (600 mg/kg, IP). CoQ10 and MMP-9 levels were analyzed using the HPLC-UV method and a commercial kit by ELISA, respectively. AFB1 increased MMP-9 level and decreased CoQ10 level compared to the control group. After dexamethasone and minocycline administration, there is no increase in CoQ10 level, which is caused by AFB1. However, dexamethasone and minocycline combined with NAC+vitamin E caused significant increases in CoQ10 levels. Dexamethasone and minocycline alone and combined with NAC+vitamin E decreased MMP-9 levels compared to the single AFB1 treated group. The use of MMPs inhibitors and oxidative stress-reducing agents is anticipated to be beneficial in the poisoning with AFB1.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33482694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular screening of gastric Helicobacter pullorum recovered from different avian species in Egypt. 埃及不同禽种胃幽门螺杆菌的分子筛选。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2022-09-01 DOI: 10.24425/pjvs.2022.142019

R Elhelw, M Elhariri, H M Salem, H S Khalefa, D A Hamza, Z S Ahmed

{"title":"Molecular screening of gastric Helicobacter pullorum recovered from different avian species in Egypt.","authors":"R Elhelw, M Elhariri, H M Salem, H S Khalefa, D A Hamza, Z S Ahmed","doi":"10.24425/pjvs.2022.142019","DOIUrl":"https://doi.org/10.24425/pjvs.2022.142019","url":null,"abstract":"Helicobacter pullorum (H. pullorum) is a bacterium that colonizes the intestines of poultry and causes gastroenteritis. Because these species are known as human and/or animal pathogens, identification of H. pullorum is becoming increasingly necessary. The bacterium has been linked to colitis and hepatitis in humans after being transmitted by infected meat consumption. Misdiagnosis of other enteric zoonotic pathogens such as Campylobacter and other Helicobacter species makes the diagnosis of H. pullorum extremely difficult. This study focused on the molecular detection of H. pullorum from the stomach (proventriculus and gizzard) of different avian species as new target organs for detection and transmission between avian species. Proventriculus and gizzards were obtained from 40 freshly dead chickens and resident wild birds (n=40). Diarrhea was found in the farms that were surveyed. DNA was extracted from all collected samples to conduct PCR amplification. The samples were screened for Helicobacter genus-specific 16s using C97 and C05 primers. To confirm the existence of H. pullorum, the positive samples were sequenced. H. pullorum was recorded in two out of 40 chicken samples. In addition, H. pullorum was recorded in one out of 40 resident wild birds. The 16S rRNA gene sequence for Helicobacter genus-specific in poultry and wild birds showed a 100% homology. In conclusion, broiler chickens and resident wild birds are possible reservoirs for H. pullorum, according to this report, and possibly act as a source of infection for humans via the food supply.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33481110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effect of alkaloid berberine on the susceptibility of nontuberculous mycobacteria to antibiotics. 生物碱小檗碱对非结核分枝杆菌抗生素敏感性的影响。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2022-09-01 DOI: 10.24425/pjvs.2022.142034

K Puk, L Guz

引用次数: 0

Vitamin E protected the mouse adrenal gland against immobilization stress. 维生素E对小鼠肾上腺抗固定应激有保护作用。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2022-09-01 DOI: 10.24425/pjvs.2022.142029

F Aşır, Y Nergiz, A Pala

{"title":"Vitamin E protected the mouse adrenal gland against immobilization stress.","authors":"F Aşır, Y Nergiz, A Pala","doi":"10.24425/pjvs.2022.142029","DOIUrl":"https://doi.org/10.24425/pjvs.2022.142029","url":null,"abstract":"In this study, we aimed to investigate the effects of vitamin E on mouse adrenal glands in immobilization stress. Twenty-eight male, 10-week-old, BALB/C mice weighing 30-45 grams were divided into four groups. Mice were placed in a cage where no movement was allowed 6 hours/day for 7 days for immobilization stress. 10 ml/kg vitamin E was administered orogastrically 1 hour before immobilization stress in the vitamin E and stress+vitamin E group. At the end of the 7th day, all the animals were subjected to elevated-plus maze (anxiety) and forced swimming (depression) tests. Left adrenal glands were dissected for routine paraffin tissue embedding protocol. Adrenal sections were stained with hematoxylin-eosin and Azan. Malonaldehyde (MDA) levels were also measured in the adrenal tissues. Anxiety level (0.023), depression level (p=0.042) and MDA values (p=0.01) were significantly increased in the stress group. Histological sections of the stress group showed cortical atrophy, medullary hypertrophy, vascular dilation and hemorrhage. Azan staining revealed a thinned capsule and corticomedullary fibrosis in the stress group. Pathologies induced by immobilization stress were mostly reversed after vitamin E administration. The results suggested that vitamin E alleviates adverse effects of immobilization stress (oxidative, behavioral and histopathologic changes) in mice.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33481514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

The application of videodermoscopic examination in assessing the skin and its products in pure Arabian horses in the summer and winter seasons. 视频皮肤镜检查在夏冬两季纯阿拉伯马皮肤及其制品评估中的应用。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2022-09-01 DOI: 10.24425/pjvs.2022.142024

A Pomorska-Zniszczyńska, M P Szczepanik, G Kalisz, N Adamczyk, M Tarach, M Sobuś, B Abramowicz

{"title":"The application of videodermoscopic examination in assessing the skin and its products in pure Arabian horses in the summer and winter seasons.","authors":"A Pomorska-Zniszczyńska, M P Szczepanik, G Kalisz, N Adamczyk, M Tarach, M Sobuś, B Abramowicz","doi":"10.24425/pjvs.2022.142024","DOIUrl":"https://doi.org/10.24425/pjvs.2022.142024","url":null,"abstract":"Videodermoscopic examinations have only recently started to be used in veterinary medicine and usually involve a small group of animals. The aim of this study was to compare specific dermoscopic parameters in selected areas of the body of Arabian horses in the summer and winter seasons. The research was conducted between 2018-2019. The procedure was performed on the left side of the horse's body in seven areas: head, neck, chest, flank, rump, mane, and tail. 42 purebred Arabian horses aged 1-25 (median), 39 mares and 3 stallions were qualified for the study. An Italian Video-Dermascope 7 (Medici Medical SRL with the 3 VIDIX 5Mpx camera and the VX1 overlay - Contact type cap Ø 3.5 cm) was used in the dermatoscopic evaluation. Specialist Cellsens Olympus software for analysing microscopic images was used to perform the measurements. Regardless of the area assessed, the skin in the summer months is darker than in the winter months. Hair thickness ranged from 44.82 (chest in summer) to 87.45 um (mane in winter). Regarding hair density, the number of hairs in the field of view of the dermatoscope ranged from 990 (summer mane) to 3680 (head in winter). Our research showed that the season of the year influences the amount of hair without significantly affecting its thickness.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33482735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Desmopathy of the collateral ligaments of the equine distal interphalangeal joint - a prevalence in Poland between 2016-2019. 马远端指间关节副韧带粘连病——2016-2019年在波兰流行。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2022-06-01 DOI: 10.24425/pjvs.2022.141816

M Mieszkowska, J Abako, M Mieszkowski, Y Zhalniarovich

{"title":"Desmopathy of the collateral ligaments of the equine distal interphalangeal joint - a prevalence in Poland between 2016-2019.","authors":"M Mieszkowska, J Abako, M Mieszkowski, Y Zhalniarovich","doi":"10.24425/pjvs.2022.141816","DOIUrl":"https://doi.org/10.24425/pjvs.2022.141816","url":null,"abstract":"Desmopathy of the collateral ligaments of the equine interphalangeal joint is caused by a combination of factors, including hoof shape, excessive loading and ground surface. This complex problem poses a diagnostic challenge due to the non-specificity of perineural analgesia and the limitations associated with the most popular imaging methods such as radiography and ultrasonography. The aim of this study was to retrospectively determine the prevalence of desmopathy of the collateral ligaments of the equine distal interphalangeal joint in Poland between 2016 and 2019, and to establish the frequency and type of the associated pathologies. Desmopathy of the collateral ligaments of the distal interphalangeal joint was diagnosed in 14% of 152 horses examined by magnetic resonance imaging (MRI). In 64% of the cases, other changes were observed in the equine digit, and in 36% of the cases, desmopathy was the only diagnosed problem. Desmopathy of the collateral ligaments is not a frequently reported pathology in the distal part of the equine limb. The diagnostic difficulties described in this article suggest that considerable caution should be exercised when formulating the final diagnosis, prognosis and treatment options.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40526791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Presence of Coxiella burnetii in dairy cattle and farms in the Czech Republic. 在捷克共和国的奶牛和农场中存在伯纳氏科希氏菌。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2022-06-01 DOI: 10.24425/pjvs.2022.141807

A Dobos, I Fodor, T Tekin, D Đuričić, M Samardzija

引用次数: 0