在捷克共和国的奶牛和农场中存在伯纳氏科希氏菌。

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Polish journal of veterinary sciences Pub Date : 2022-06-01 DOI:10.24425/pjvs.2022.141807

A Dobos, I Fodor, T Tekin, D Đuričić, M Samardzija

{"title":"在捷克共和国的奶牛和农场中存在伯纳氏科希氏菌。","authors":"A Dobos, I Fodor, T Tekin, D Đuričić, M Samardzija","doi":"10.24425/pjvs.2022.141807","DOIUrl":null,"url":null,"abstract":"The aims of this study were to evaluate the prevalence of Coxiella burnetii on both herd and animal level based on ELISA and PCR tests. Antibodies to C. burnetii were detected in 22 out of the 24 bulk tank milk samples (91.6%) tested by ELISA and the IS1111 element of C. burnetii was detected in 10 out of the 24 samples (41.6%) by real-time polymerase chain reaction (PCR). ELISA testing showed individual seropositivity in 67 out of the 165 cows (40.6%) examined in 24 dairy cattle farms in different parts of the Czech Republic. Our study revealed that the prevalence of C. burnetii has increased substantially in the Czech Republic over the past 30 years, and that the causative agent is a potential risk factor for some reproductive problems in dairy farms and a possible risk factor for human infection.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Presence of Coxiella burnetii in dairy cattle and farms in the Czech Republic.\",\"authors\":\"A Dobos, I Fodor, T Tekin, D Đuričić, M Samardzija\",\"doi\":\"10.24425/pjvs.2022.141807\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The aims of this study were to evaluate the prevalence of Coxiella burnetii on both herd and animal level based on ELISA and PCR tests. Antibodies to C. burnetii were detected in 22 out of the 24 bulk tank milk samples (91.6%) tested by ELISA and the IS1111 element of C. burnetii was detected in 10 out of the 24 samples (41.6%) by real-time polymerase chain reaction (PCR). ELISA testing showed individual seropositivity in 67 out of the 165 cows (40.6%) examined in 24 dairy cattle farms in different parts of the Czech Republic. Our study revealed that the prevalence of C. burnetii has increased substantially in the Czech Republic over the past 30 years, and that the causative agent is a potential risk factor for some reproductive problems in dairy farms and a possible risk factor for human infection.\",\"PeriodicalId\":20366,\"journal\":{\"name\":\"Polish journal of veterinary sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2022-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Polish journal of veterinary sciences\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.24425/pjvs.2022.141807\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polish journal of veterinary sciences","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.24425/pjvs.2022.141807","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

本研究的目的是在ELISA和PCR检测的基础上，评估伯纳氏克希菌在畜群和动物水平上的流行情况。ELISA检测的24份散装罐乳样品中有22份(91.6%)检测到伯纳蒂胞杆菌抗体，实时聚合酶链反应(PCR)检测到伯纳蒂胞杆菌IS1111元件的有10份(41.6%)。ELISA检测显示，在捷克共和国不同地区的24个奶牛场检测的165头奶牛中，有67头(40.6%)呈个体血清阳性。我们的研究表明，在过去的30年里，伯纳蒂胞杆菌在捷克共和国的流行率大幅上升，病原体是奶牛场某些生殖问题的潜在危险因素，也是人类感染的可能危险因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Presence of Coxiella burnetii in dairy cattle and farms in the Czech Republic.

The aims of this study were to evaluate the prevalence of Coxiella burnetii on both herd and animal level based on ELISA and PCR tests. Antibodies to C. burnetii were detected in 22 out of the 24 bulk tank milk samples (91.6%) tested by ELISA and the IS1111 element of C. burnetii was detected in 10 out of the 24 samples (41.6%) by real-time polymerase chain reaction (PCR). ELISA testing showed individual seropositivity in 67 out of the 165 cows (40.6%) examined in 24 dairy cattle farms in different parts of the Czech Republic. Our study revealed that the prevalence of C. burnetii has increased substantially in the Czech Republic over the past 30 years, and that the causative agent is a potential risk factor for some reproductive problems in dairy farms and a possible risk factor for human infection.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Polish journal of veterinary sciences 农林科学-兽医学

CiteScore

1.40

自引率

0.00%

发文量

审稿时长

12-24 weeks

期刊介绍： Polish Journal of Veterinary Sciences accepts short communications, original papers and review articles from the field of, widely understood, veterinary sciences - basic, clinical, environmental, animal-origin food hygiene, feed hygiene, etc.