Journal of Tissue Engineering and Regenerative Medicine最新文献_第7页

Effective decellularization of human skin tissue for regenerative medicine by supercritical carbon dioxide technique 超临界二氧化碳技术用于再生医学的人体皮肤组织的有效脱细胞

IF 3.3 3区生物学

Journal of Tissue Engineering and Regenerative Medicine Pub Date : 2022-11-08 DOI: 10.1002/term.3359

Nguyen Ngan Giang, Xuan-Tung Trinh, Jeonghun Han, Pham Ngoc Chien, Jua Lee, Seong-Rae Noh, Yongwoo Shin, Sun-Young Nam, Chan-Yeong Heo

{"title":"Effective decellularization of human skin tissue for regenerative medicine by supercritical carbon dioxide technique","authors":"Nguyen Ngan Giang, Xuan-Tung Trinh, Jeonghun Han, Pham Ngoc Chien, Jua Lee, Seong-Rae Noh, Yongwoo Shin, Sun-Young Nam, Chan-Yeong Heo","doi":"10.1002/term.3359","DOIUrl":"https://doi.org/10.1002/term.3359","url":null,"abstract":"Allotransplantation, performed using an acellular dermal matrix (ADM), plays a significant role in the cultivation of constituted and damaged organs in clinical. Herein, we fabricated an innovative ADM for allografting derived from decellularized human skin by utilizing the supercritical fluid of carbon dioxide to eliminate immunogenic components. By using histological staining, the ADM product demonstrated the successful removal of cellular constituents without exerting any harmful influence on the extracellular matrix. The results from DNA electrophoresis also supported this phenomenon by showing the complete DNA removal in the product, accompanied by the absence of Major Histocompatibility Complex 1, which suggested the supercritical fluid is an effective method for cellular withdrawal. Moreover, the mechanical property of the ADM products, which showed similarity to that of native skin, displayed great compatibility for using our human-derived ADM as an allograft in clinical treatment. Specifically, the cell viability demonstrated the remarkable biocompatibility of the product to human bio-cellular environment which was noticeably higher than that of other products. Additionally, the significant increase in the level of growth factors such as vascular endothelial growth factor, urokinase-type plasminogen activator receptor, granulocyte-macrophage colony-stimulating factor suggested the ability to stimulate cellular processes, proving the products to be innovative in the field of regeneration when applied to clinical in the future. This study provides a thoroughly extensive analysis of the new ADM products, enabling them to be applied in industrial and clinical treatment.","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"16 12","pages":"1196-1207"},"PeriodicalIF":3.3,"publicationDate":"2022-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6158248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Matricellular protein Tenascin-C enhances mesenchymal stem cell angiogenic and wound healing efficacy under ischemic conditions 基质细胞蛋白Tenascin-C增强缺血条件下间充质干细胞血管生成和伤口愈合的功效

IF 3.3 3区生物学

Journal of Tissue Engineering and Regenerative Medicine Pub Date : 2022-11-08 DOI: 10.1002/term.3367

Kyle Sylakowski, Peter Hwang, Amritha Justin, Hanshuang Shao, Diana Whaley, Yadong Wang, Alan Wells

{"title":"Matricellular protein Tenascin-C enhances mesenchymal stem cell angiogenic and wound healing efficacy under ischemic conditions","authors":"Kyle Sylakowski, Peter Hwang, Amritha Justin, Hanshuang Shao, Diana Whaley, Yadong Wang, Alan Wells","doi":"10.1002/term.3367","DOIUrl":"https://doi.org/10.1002/term.3367","url":null,"abstract":"Human mesenchymal stem cells/multipotent stromal cells (MSCs) hold great promise in aiding wound healing through their ability to modulate all phases of repair and regeneration, most notably their secretion of pro-regenerative paracrine factors. However, MSC clinical utility is hindered by poor survival rates post-transplantation due to the harsh microenvironment in injured tissue. Previous work has shown that the matricellular protein Tenascin-C (TNC) provides survival signaling to MSCs via the epidermal growth factor receptor by restricting its activation at the plasma membrane, resulting in enhanced prosurvival signals. Herein, we investigate how TNC influences MSC survival and MSC-mediated promotion of the wound healing process. This study examined the survival and angiogenic potential of MSCs cultured on TNC-coated surfaces under ischemic duress in vitro. We also assessed the angiogenic and wound healing outcomes of MSC + TNC in vivo using a CXCR3−/− mouse model that exhibits a delayed healing phenotype within the tissue replacement phase of repair. We found that MSCs in the presence of TNC exhibit higher levels of angiogenic-promoting processes, collagen maturation, and an overall better wound healing outcome than MSCs administered alone. This was seen in vitro in terms of enhanced tube formation. In vivo, the MSCs in the presence of TNC stabilized with a coacervate delivery system resulted in more regenerative wounds with accelerated maturation of the dermis. These findings suggest the coupling of TNC to MSCs as a promising tool for future MSC-ECM combinatorial therapies for wound healing applications.","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"16 12","pages":"1249-1260"},"PeriodicalIF":3.3,"publicationDate":"2022-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6050434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Three-dimensional wet electrospun scaffold system for the differentiation of adipose-derived mesenchymal stem cells to islet-like clusters 用于脂肪来源的间充质干细胞向胰岛样干细胞簇分化的三维湿式电纺丝支架系统

IF 3.3 3区生物学

Journal of Tissue Engineering and Regenerative Medicine Pub Date : 2022-11-08 DOI: 10.1002/term.3366

Jijo Wilson, V. G. Rahul, Lynda V. Thomas, Prabha D. Nair

{"title":"Three-dimensional wet electrospun scaffold system for the differentiation of adipose-derived mesenchymal stem cells to islet-like clusters","authors":"Jijo Wilson, V. G. Rahul, Lynda V. Thomas, Prabha D. Nair","doi":"10.1002/term.3366","DOIUrl":"https://doi.org/10.1002/term.3366","url":null,"abstract":"Stem cell-derived islet-like clusters (ILCs) are an alternative source of pancreatic beta cells for the treatment of diabetic mellitus. An ideal 3D culture platform for the generation of ILCs of desired cluster size is a challenge due to the clustering of islet cells in the 2D culture systems. The islet cells cultured in 2D conditions produce clusters of large size, which are less efficient in terms of insulin secretion and viability. In this study, we report that ILCs formed on a PCL-based wet electrospun fibrous scaffold with larger pore size produced clusters of the desired size, compared to that cultured on a conventional electrospun sheet. The collagen functionalization on this wet electrospun polycaprolactone (PCL) scaffold showed enhanced insulin secretion and cell viability compared to the non-functionalized or conventionally electrospun PCL scaffold. The collagen-coated wet electrospun 3D scaffold produced ILCs of cluster diameter 70 ± 20 μm and the conventionally electrospun PCL sheet produced larger ILC clusters of diameter 300 ± 10 μm. Hence the results indicate the collagen-functionalized wet electrospun scaffold system could be a potential scaffold for islet tissue engineering.","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"16 12","pages":"1276-1283"},"PeriodicalIF":3.3,"publicationDate":"2022-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6145522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Fabrication of BMP-2-peptide–Deferoxamine- and QK-peptide-functionalized nanoscaffolds and their application for bone defect treatment bmp -2肽-去铁胺和qk -肽功能化纳米支架的制备及其在骨缺损治疗中的应用

IF 3.3 3区生物学

Journal of Tissue Engineering and Regenerative Medicine Pub Date : 2022-11-08 DOI: 10.1002/term.3364

Zecheng Li, Shi Cheng, Ang Li, Chengchao Song, Anlong Jiang, Fangxing Xu, Hui Chi, Jinglong Yan, Guanghua Chen

{"title":"Fabrication of BMP-2-peptide–Deferoxamine- and QK-peptide-functionalized nanoscaffolds and their application for bone defect treatment","authors":"Zecheng Li, Shi Cheng, Ang Li, Chengchao Song, Anlong Jiang, Fangxing Xu, Hui Chi, Jinglong Yan, Guanghua Chen","doi":"10.1002/term.3364","DOIUrl":"https://doi.org/10.1002/term.3364","url":null,"abstract":"The microenvironment in the healing process of large bone defects requires suitable conditions to promote osteogenesis and angiogenesis. Coaxial electrospinning is a mature method in bone tissue engineering (BTE) and allows functional modification. Appropriate modification methods can be used to improve the bioactivity of scaffolds for BTE. In this study, coaxial electrospinning with QK peptide (a Vascular endothelial growth factor mimetic peptide) and BMP-2 peptide–DFO (BD) was performed to produce double-modified PQBD scaffolds with vascularizing and osteogenic features. The morphology of coaxially electrospun scaffolds was verified by scanning electron microscopy (SEM) and transmission electron microscopy. Laser scanning confocal microscopy and Fourier transform infrared spectroscopy confirmed that BD covalently bound to the surface of the P and PQ scaffolds. In vitro, the PQBD scaffold promoted the adhesion and proliferation of bone marrow stromal cells (BMSCs). Both QK peptide and BD showed sustainable release and preservation of biological activity, enhancing the osteogenic differentiation of BMSCs and the migration of human umbilical vein endothelial cells and promoting angiogenesis. The combined ability of these factors to promote osteogenesis and angiogenesis is superior to that of each alone. In vivo, the PQBD scaffold was implanted into the bone defect, and after 8 weeks, the defect area was almost completely covered by new bone tissue. Histology showed more mature bone tissue and more blood vessels. PQBD scaffolds promote both angiogenesis and osteogenesis, offering a promising approach to enhance bone regeneration in the treatment of large bone defects.","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"16 12","pages":"1223-1237"},"PeriodicalIF":3.3,"publicationDate":"2022-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6158256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Application of amniotic membranes in reconstructive surgery of internal organs—A systematic review and meta-analysis 羊膜在内脏器官重建手术中的应用——系统综述和荟萃分析

IF 3.3 3区生物学

Journal of Tissue Engineering and Regenerative Medicine Pub Date : 2022-11-04 DOI: 10.1002/term.3357

Lennart P. Maljaars, Sohayla Bendaoud, Arnoud W. Kastelein, Zeliha Guler, Carlijn R. Hooijmans, Jan-Paul W. R. Roovers

{"title":"Application of amniotic membranes in reconstructive surgery of internal organs—A systematic review and meta-analysis","authors":"Lennart P. Maljaars, Sohayla Bendaoud, Arnoud W. Kastelein, Zeliha Guler, Carlijn R. Hooijmans, Jan-Paul W. R. Roovers","doi":"10.1002/term.3357","DOIUrl":"https://doi.org/10.1002/term.3357","url":null,"abstract":"Amniotic membrane (AM) has great potential as a scaffold for tissue regeneration in reconstructive surgery. To date, no systematic review of the literature has been performed for the applications of AM in wound closure of internal organs. Therefore, in this systematic review and meta-analysis, we summarize the literature on the safety and efficacy of AM for the closure of internal organs. A systematic search was performed in MEDLINE-PubMed database and OVID Embase to retrieve human and controlled animal studies on wound closure of internal organs. The Cochrane Risk of Bias tool for randomized clinical trials and the SYRCLE risk of bias tool for animal studies were used. Meta-analyses (MAs) were conducted for controlled animal studies to assess efficacy of closure, mortality and complications in subjects who underwent surgical wound closure in internal organs with the application of AM. Sixty references containing 26 human experiments and 36 animal experiments were included. The MAs of the controlled animal studies showed comparable results with regard to closure, mortality and complications, and suggested improved mechanical strength and lower inflammation scores after AM application when compared to standard surgical closure techniques. This systematic review and MAs demonstrate that the application of AM to promote wound healing of internal organs appears to be safe, efficacious, and feasible.","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"16 12","pages":"1069-1090"},"PeriodicalIF":3.3,"publicationDate":"2022-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/term.3357","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6124630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The inflammasome in biomaterial-driven immunomodulation 生物材料驱动免疫调节中的炎性体

IF 3.3 3区生物学

Journal of Tissue Engineering and Regenerative Medicine Pub Date : 2022-11-03 DOI: 10.1002/term.3361

Daniela P. Vasconcelos, Artur P. águas, Judite N. Barbosa

{"title":"The inflammasome in biomaterial-driven immunomodulation","authors":"Daniela P. Vasconcelos, Artur P. águas, Judite N. Barbosa","doi":"10.1002/term.3361","DOIUrl":"https://doi.org/10.1002/term.3361","url":null,"abstract":"Inflammasomes are intracellular structures formed upon the assembly of several proteins that have a considerable size and are very important in innate immune responses being key players in host defense. They are assembled after the perception of pathogens or danger signals. The activation of the inflammasome pathway induces the production of high levels of the pro-inflammatory cytokines Interleukin (IL)-1β and IL-18 through the caspase activation. The procedure for the implantation of a biomaterial causes tissue injury, and the injured cells will secrete danger signals recognized by the inflammasome. There is growing evidence that the inflammasome participates in a number of inflammatory processes, including pathogen clearance, chronic inflammation and tissue repair. Therefore, the control of the inflammasome activity is a promising target in the development of capable approaches to be applied in regenerative medicine. In this review, we revisit current knowledge of the inflammasome in the inflammatory response to biomaterials and point to the yet underexplored potential of the inflammasome in the context of immunomodulation.","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"16 12","pages":"1109-1120"},"PeriodicalIF":3.3,"publicationDate":"2022-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/term.3361","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6064565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Nanofibrous polytetrafluoroethylene/poly(ε-caprolactone) membrane with hierarchical structures for vascular patch 具有层次化结构的聚四氟乙烯/聚(ε-己内酯)纳米纤维膜

IF 3.3 3区生物学

Journal of Tissue Engineering and Regenerative Medicine Pub Date : 2022-11-03 DOI: 10.1002/term.3354

Yulu Liu, Ya Liu, Zhiyuan Bai, Dongfang Wang, Yiyang Xu, Qian Li

{"title":"Nanofibrous polytetrafluoroethylene/poly(ε-caprolactone) membrane with hierarchical structures for vascular patch","authors":"Yulu Liu, Ya Liu, Zhiyuan Bai, Dongfang Wang, Yiyang Xu, Qian Li","doi":"10.1002/term.3354","DOIUrl":"https://doi.org/10.1002/term.3354","url":null,"abstract":"With the prevalence of cardiovascular diseases, developing cardiovascular supplements is becoming increasingly urgent. The ability of cells to rapidly adhere and proliferate to achieve endothelialization is extremely important for vascular grafts. In this work, we electrospun polytetrafluoroethylene (PTFE) nanofibrous membranes and used induced crystallization to manufacture poly(ε-caprolactone) (PCL) shish-kebab microstructures on PTFE nanofibers to overcome the inertness of PTFE, and promote cell adhesion and proliferation. PCL lamella periodically grew on the surface of PTFE nanofibers yielding a hierarchical structure, which improved the biocompatibility and mechanical properties of the PTFE nanofibrous membrane. The deposition of PCL lamella improved the hydrophilicity of electrospun PTFE nanofibers membrane, leading to good cell proliferation and adhesion. Also, due to the surface inertness of the substrate material PTFE, this PTFE/PCL composite film has good anti-platelet adhesion properties. Furthermore, cell proliferation could be regulated by controlling the integrity of the PCL crystal network. The vascular patch showed similar mechanical properties to natural blood vessels, providing a new strategy for vascular tissue engineering.","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"16 12","pages":"1163-1172"},"PeriodicalIF":3.3,"publicationDate":"2022-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6064567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stem cell-based strategies for skeletal muscle tissue engineering 基于干细胞的骨骼肌组织工程策略

IF 3.3 3区生物学

Journal of Tissue Engineering and Regenerative Medicine Pub Date : 2022-10-12 DOI: 10.1002/term.3355

Christofer Baldwin, Johntaehwan Kim, Srikanth Sivaraman, Raj R. Rao

引用次数: 1

Accelerated vascularization of a novel collagen hydrogel dermal template 一种新型胶原水凝胶真皮模板的加速血管化

IF 3.3 3区生物学

Journal of Tissue Engineering and Regenerative Medicine Pub Date : 2022-10-11 DOI: 10.1002/term.3356

Adam Weisel, Rachael Cohen, Jason A. Spector, Yulia Sapir-Lekhovitser

{"title":"Accelerated vascularization of a novel collagen hydrogel dermal template","authors":"Adam Weisel, Rachael Cohen, Jason A. Spector, Yulia Sapir-Lekhovitser","doi":"10.1002/term.3356","DOIUrl":"https://doi.org/10.1002/term.3356","url":null,"abstract":"Full thickness skin loss is a debilitating problem, most commonly reconstructed using split thickness skin grafts (STSG), which do not reconstitute normal skin thickness and often result in suboptimal functional and esthetic outcomes that diminish a patient's quality of life. To address the minimal dermis present in most STSG, engineered dermal templates were developed that can induce tissue ingrowth and the formation of neodermal tissue. However, clinically available dermal templates have many shortcomings including a relatively slow rate and degree of neovascularization (∼2–4 weeks), resulting in multiple dressing changes, prolonged immobilization, and susceptibility to infection. Presented herein is a novel composite hydrogel scaffold that optimizes a unique scaffold microarchitecture with native hydrogel properties and mechanical cues ideal for promoting neovascularization, tissue regeneration, and wound healing. In vitro analysis demonstrated the unique combination of improved mechanical attributes with native hydrogel properties that promotes cell invasion and remodeling within the scaffold. In a novel 2-stage rat model of full thickness skin loss that closely mimics clinical practice, the composite hydrogel induced rapid cell infiltration and neovascularization, creating a healthy neodermis after only 1 week onto which a skin graft could be placed. The scaffold also elicited a gradual and favorable immune response, resulting in more efficient integration into the host. We have developed a dermal scaffold that utilizes simple but unique collagen hydrogel architectural cues that rapidly induces the formation of stable, functional neodermal tissue, which holds tremendous promise for the treatment of full thickness skin loss.","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"16 12","pages":"1173-1183"},"PeriodicalIF":3.3,"publicationDate":"2022-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5810267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In vitro testing and efficacy of poly-lactic acid coating incorporating antibiotic loaded coralline bioceramic on Ti6Al4V implant against Staphylococcus aureus 载抗生素珊瑚生物陶瓷聚乳酸包被Ti6Al4V种植体抗金黄色葡萄球菌的体外试验及效果观察

IF 3.3 3区生物学

Journal of Tissue Engineering and Regenerative Medicine Pub Date : 2022-10-07 DOI: 10.1002/term.3353

Ipek Karacan, Besim Ben-Nissan, Jerran Santos, Stanley Yiu, Peta Bradbury, Stella M. Valenzuela, Joshua Chou

{"title":"In vitro testing and efficacy of poly-lactic acid coating incorporating antibiotic loaded coralline bioceramic on Ti6Al4V implant against Staphylococcus aureus","authors":"Ipek Karacan, Besim Ben-Nissan, Jerran Santos, Stanley Yiu, Peta Bradbury, Stella M. Valenzuela, Joshua Chou","doi":"10.1002/term.3353","DOIUrl":"https://doi.org/10.1002/term.3353","url":null,"abstract":"Biofilm formation on an implant surface is most commonly caused by the human pathogenic bacteria Staphylococcus aureus, which can lead to implant related infections and failure. It is a major problem for both implantable orthopedic and maxillofacial devices. The current antibiotic treatments are typically delivered orally or in an injectable form. They are not highly effective in preventing or removing biofilms, and they increase the risk of antibiotic resistance of bacteria and have a dose-dependent negative biological effect on human cells. Our aim was to improve current treatments via a localized and controlled antibiotic delivery-based implant coating system to deliver the antibiotic, gentamicin (Gm). The coating contains coral skeleton derived hydroxyapatite powders (HAp) that act as antibiotic carrier particles and have a biodegradable poly-lactic acid (PLA) thin film matrix. The system is designed to prevent implant related infections while avoiding the deleterious effects of high concentration antibiotics in implants on local cells including primary human adipose derived stem cells (ADSCs). Testing undertaken in this study measured the rate of S. aureus biofilm formation and determined the growth rate and proliferation of ADSCs. After 24 h, S. aureus biofilm formation and the percentage of live cells found on the surfaces of all 5%–30% (w/w) PLA-Gm-(HAp-Gm) coated Ti6Al4V implants was lower than the control samples. Furthermore, Ti6Al4V implants coated with up to 10% (w/w) PLA-Gm-(HAp-Gm) did not have noticeable Gm related adverse effect on ADSCs, as assessed by morphological and surface attachment analyses. These results support the use and application of the antibacterial PLA-Gm-(HAp-Gm) thin film coating design for implants, as an antibiotic release control mechanism to prevent implant-related infections.","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"16 12","pages":"1149-1162"},"PeriodicalIF":3.3,"publicationDate":"2022-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6123225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1