Plant Genetic Resources: Characterization and Utilization最新文献_第9页

Rice blast resistance gene profiling of Thai, Japanese and International rice varieties using gene-specific markers 利用基因特异性标记分析泰国、日本和国际水稻品种抗稻瘟病基因

IF 1.1 4区生物学

Plant Genetic Resources: Characterization and Utilization Pub Date : 2022-05-12 DOI: 10.1017/s1479262122000089

Wattanaporn Teerasan, Pattaraborn Moonsap, Apinya Longya, K. Damchuay, S. Ito, P. Tasanasuwan, Sureeporn Kate-ngam, C. Jantasuriyarat

{"title":"Rice blast resistance gene profiling of Thai, Japanese and International rice varieties using gene-specific markers","authors":"Wattanaporn Teerasan, Pattaraborn Moonsap, Apinya Longya, K. Damchuay, S. Ito, P. Tasanasuwan, Sureeporn Kate-ngam, C. Jantasuriyarat","doi":"10.1017/s1479262122000089","DOIUrl":"https://doi.org/10.1017/s1479262122000089","url":null,"abstract":"\u0000 Rice blast disease, caused by Magnaporthe oryzae, is one of the most damaging diseases of rice worldwide. Cultivation of rice varieties carrying resistance genes is the most economic and successful strategy to control the disease. In this study, 451 rice varieties from around the world including 363 Thai landrace rice varieties, 21 Thai improved rice varieties, 43 Japanese rice varieties and 24 worldwide rice varieties were screened by PCR technique using gene-specific markers for 10 rice blast resistance genes: Pi9, Piz-t, Pi50, Pigm(t), Pid2, Pid3, Pia, Pik, Pi54 and Pita. The results showed that 382 (99.48%) Thai rice varieties have at least one resistance gene and two rice varieties, ‘Hom’ and ‘Bak muay’, contained eight out of ten screened rice blast resistance genes. 320 rice varieties (83.33%) contained three or more rice blast resistance genes. The frequency of the rice blast resistance gene ranges from 87.76–9.64 per cent, of which the Pid3 gene has the highest frequency and the Pi54 gene has the lowest frequency. Two major resistance genes, found in Japanese rice varieties, are the Pik gene (76.74%) and the Pi9 gene (72.09%). While two major resistance genes, found in the international rice varieties are the Pi9 gene (66.67%) and the Pi54 gene (62.50%). The disease resistance gene profile of each rice variety obtained from this study will benefit the rice blast resistant breeding programme in the future.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83697832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Characterization of novel low-molecular-weight glutenin subunit genes from the diploid wild wheat relative Aegilops umbellulata 野生小麦二倍体亲缘小叶麦麸蛋白低分子亚基基因的研究

IF 1.1 4区生物学

Plant Genetic Resources: Characterization and Utilization Pub Date : 2022-05-12 DOI: 10.1017/s1479262122000016

Wenyang Wang, Wenjun Ji, Lihua Feng, S. Ning, Zhongwei Yuan, M. Hao, Lianquan Zhang, Zehong Yan, Bihua Wu, Dengcai Liu, Lin Huang

{"title":"Characterization of novel low-molecular-weight glutenin subunit genes from the diploid wild wheat relative Aegilops umbellulata","authors":"Wenyang Wang, Wenjun Ji, Lihua Feng, S. Ning, Zhongwei Yuan, M. Hao, Lianquan Zhang, Zehong Yan, Bihua Wu, Dengcai Liu, Lin Huang","doi":"10.1017/s1479262122000016","DOIUrl":"https://doi.org/10.1017/s1479262122000016","url":null,"abstract":"\u0000 Low molecular weight glutenin subunits (LWM-GSs) play a crucial role in determining wheat flour processing quality. In this work, 35 novel LMW-GS genes (32 active and three pseudogenes) from three Aegilops umbellulata (2n = 2x = 14, UU) accessions were amplified by allelic-specific PCR. We found that all LMW-GS genes had the same primary structure shared by other known LMW-GSs. Thirty-two active genes encode 31 typical LMW-m-type subunits. The MZ424050 possessed nine cysteine residues with an extra cysteine residue located in the last amino acid residue of the conserved C-terminal III, which could benefit the formation of larger glutenin polymers, and therefore may have positive effects on dough properties. We have found extensive variations which were mainly resulted from single-nucleotide polymorphisms (SNPs) and insertions and deletions (InDels) among the LMW-GS genes in Ae. umbellulata. Our results demonstrated that Ae. umbellulata is an important source of LMW-GS variants and the potential value of the novel LMW-GS alleles for wheat quality improvement.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74381519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological description based on DUS characters and molecular characterization of ‘Sikkim Primitive’ maize: an endangered unique genetic resource 濒危独特遗传资源“锡金原始”玉米DUS特征形态描述及分子特征

IF 1.1 4区生物学

Plant Genetic Resources: Characterization and Utilization Pub Date : 2022-05-10 DOI: 10.1017/s1479262122000065

C. Kapoor, Shweta Singh, R. Avasthe, C. Raj, Matber Singh, Hissay L. Lepcha

{"title":"Morphological description based on DUS characters and molecular characterization of ‘Sikkim Primitive’ maize: an endangered unique genetic resource","authors":"C. Kapoor, Shweta Singh, R. Avasthe, C. Raj, Matber Singh, Hissay L. Lepcha","doi":"10.1017/s1479262122000065","DOIUrl":"https://doi.org/10.1017/s1479262122000065","url":null,"abstract":"\u0000 ‘Sikkim Primitive’ (SP) maize locally known as ‘murali makkai’ in Sikkim is a unique genetic resource exhibiting prolificacy and excellent popping capacity. Status of SP has reached extinction level due to its very small population size and neglected conservation efforts in-situ. In an initial effort to conserve and revive this landrace, characterization and documentation was carried out with 31 morphologically assayed traits recorded at different growth stages along with molecular characterization with simple-sequence repeat (SSR) markers. Plants exhibited prolificacy (5–6 cobs/plant) and excellent popping capacity along with other distinct traits. Plants were tall with thin stem, loose drooping tassel with anthocyanin coloration present at the base of glumes and in brace roots. Cobs were medium sized carrying small seeds with low test weight (87.90 g). A total of 22 SSR markers show amplification in murali makkai with markers bnlg1083, umc1353, umc1128, bnlg1017, bnlg2077, umc2298 and umc2373 amplified unique amplicons ranging from 100 to 800 bp. The characterized set of traits and molecular characterization for murali makkai will facilitate in utilization for genetic improvement and maintenance of genetic purity.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84452366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Identification of potential donors for false smut resistance in elite breeding lines of rice (Oryza sativa L.) under field conditions 稻田条件下水稻优良选育品系抗假黑穗病潜在供体的鉴定

IF 1.1 4区生物学

Plant Genetic Resources: Characterization and Utilization Pub Date : 2022-05-04 DOI: 10.1017/s1479262122000041

J. S. Lore, J. Jain, Sanjay Kumar, Ishwinder Kamboj, N. Sidhu, R. Khanna, R. Kaur, G. S. Mangat

{"title":"Identification of potential donors for false smut resistance in elite breeding lines of rice (Oryza sativa L.) under field conditions","authors":"J. S. Lore, J. Jain, Sanjay Kumar, Ishwinder Kamboj, N. Sidhu, R. Khanna, R. Kaur, G. S. Mangat","doi":"10.1017/s1479262122000041","DOIUrl":"https://doi.org/10.1017/s1479262122000041","url":null,"abstract":"\u0000 False smut of rice is an emerging disease and caused severe damage to hybrids and inbred rice cultivars grown in Asian countries. The objective of the study was to quantify of false smut resistance and identification of donors in some of the advanced breeding lines and rice varieties developed at Punjab Agricultural University, Ludhiana, India. A total of 31 genotypes were evaluated for three years in two planting date per year under field conditions. The lines were categorized into short, medium and long durations based on days to flowering. False smut was quantified using different disease variables such as per cent infected panicle, number of false smut ball per plant and disease score. Disease variables were significantly and positively correlated to each other. The infected panicle ranged 0.0–75.4% was observed among the genotypes. Three advanced lines namely RGS-2 (short), RGM-3 (medium) and RGL-3 (long) showed the lowest ranged 0.0–4.9% of infected panicle as compared to susceptible checks (47.7–75.4%). The genotypes were divided into five groups according to a component of resistance. The third group had the lowest average values (3.3%) of infected panicle as compared to the fifth group with the highest values (36.2%) of the infected panicle. The overall trend of disease variables was higher in short duration genotypes as compared to medium and long durations. Weather factors such as rain fall, rainy days and high relative humidity during the flowering period were favourable for disease development. The genotypes with lower disease variables could be utilized in diseases resistance breeding programme.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85259649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic stability of cryopreserved ornamental Lilium germplasm 观赏百合超低温种质的遗传稳定性

IF 1.1 4区生物学

Plant Genetic Resources: Characterization and Utilization Pub Date : 2022-05-04 DOI: 10.1017/s147926212200003x

J. Song, J. Yi, J. Bae, Jung-ro Lee, M. Yoon, Young-yi Lee

{"title":"Genetic stability of cryopreserved ornamental Lilium germplasm","authors":"J. Song, J. Yi, J. Bae, Jung-ro Lee, M. Yoon, Young-yi Lee","doi":"10.1017/s147926212200003x","DOIUrl":"https://doi.org/10.1017/s147926212200003x","url":null,"abstract":"\u0000 The genus Lilium contains a number of ornamental crop species, which are commercially important in many countries. As they are vegetatively propagated, maintaining genetic stability is essential for their efficient conservation. In this study, we investigated the genetic stability of regenerated plants of three cultivars (L. bolanderi ‘Lenora’, L. bolanderi ‘Mount Duckling’ and L. bolanderi ‘Mount Dragon’) and one variety (L. callosum var. flavum) after cryopreservation, compared with fresh (donor) and non-cryopreserved plants using morphological traits and ISSR markers. No differences in morphological parameters including flower, stigma and pollen colour, floral spots, floral direction or polymorphic bands were observed between control (fresh and non-cryopreserved) and cryopreserved plantlets. In addition, based on the resulting UPGMA dendrogram, the four taxa were divided into different clusters. All cryopreserved, non-cryopreserved and fresh plants in each group could be grouped together in a single cluster with more than 97 or 100% similarity. The results suggest a very low level or the absence of genetic variation in terms of morphological and genetic stability among the plants regenerated after cryopreservation.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73642615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Identification of high-oleic peanut chemical mutants and functional analysis of mutated FAD2B gene 高油花生化学突变体的鉴定及突变FAD2B基因的功能分析

IF 1.1 4区生物学

Plant Genetic Resources: Characterization and Utilization Pub Date : 2022-05-04 DOI: 10.1017/s1479262122000053

H. Han, Z. Wang, X. Wang, Xiushan Sun, C. Fang, C. Wang

{"title":"Identification of high-oleic peanut chemical mutants and functional analysis of mutated FAD2B gene","authors":"H. Han, Z. Wang, X. Wang, Xiushan Sun, C. Fang, C. Wang","doi":"10.1017/s1479262122000053","DOIUrl":"https://doi.org/10.1017/s1479262122000053","url":null,"abstract":"\u0000 It is generally accepted that high-oleic crops have at least 70% oleate. As compared to their normal-oleic counterparts, oil and food products made from high-oleic peanut have better keeping quality and are much healthier. Therefore, high-oleic peanut is well recognized by processors and consumers. However, owing to the limited availability of high-oleic donors, most present-day high-oleic peanut varietal releases merely have F435 type FAD2 mutations. Through screening of a mutagenized peanut population of 15L46, a high-yielding peanut line with desirable elliptical oblong large seeds, using near infrared model for predicting oleate content in individual single seeds, high-oleic peanut mutants were identified. Sequencing FAD2A and FAD2B of the mutants along with the wild type revealed that these mutants possessed G448A FAD2A (F435 type FAD2A mutation) and G558A FAD2B (non-F435 type FAD2B mutation). Expression of the wild and mutated type FAD2B in yeast verified that the functional mutation contributed to the high-oleic phenotype in these mutants. The mutants provided additional high-oleic donors to peanut quality improvement.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82911508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of resistance against anthracnose (Colletotrichum capsici and C. gloeosporioides) in chilli landraces collected from the northeastern region of India 印度东北地区辣椒地方品种对炭疽病(辣椒炭疽病和辣椒炭疽病)抗性评价

IF 1.1 4区生物学

Plant Genetic Resources: Characterization and Utilization Pub Date : 2022-03-25 DOI: 10.1017/s1479262121000666

N. Dwivedi, Dipti S. Tirkey, S. Katoch, L. Prasad

{"title":"Evaluation of resistance against anthracnose (Colletotrichum capsici and C. gloeosporioides) in chilli landraces collected from the northeastern region of India","authors":"N. Dwivedi, Dipti S. Tirkey, S. Katoch, L. Prasad","doi":"10.1017/s1479262121000666","DOIUrl":"https://doi.org/10.1017/s1479262121000666","url":null,"abstract":"\u0000 Anthracnose or fruit rot disease caused by Colletotrichum spp. leads to substantial economic losses in chilli (Capsicum annuum L.) production worldwide. In the present study, 24 different Bhut Jolokia chilli landraces and seven Capsicum annuum cultivars have been collected from the northeastern region of India and subsequently screened under in vitro and in vivo conditions against Colletotrichum capsici and C. gloeosporiodes infections. During field evaluation, eight chilli genotypes (CC0164, CC0165, CC0191, CC0192, CC0202, CC0206, CC0209 and CC0218) were highly resistant and 12 genotypes (CC0154, CC0179, CC0181, CC0183, CC0186, CC0189, CC0193, CC0198, CC0205, CC0210, CC0213 and CC0217) were found in resistant category against C. capsici infection. During in-vitro germplasm evaluation, 11 and 12 landraces were found to be highly resistant to C. capsici and C. gloeosporioides infections, respectively. According to the findings, the majority of Bhut Jolokia chilli landraces are resistant to anthracnose. Given the difficulties farmers experience as a result of excessive use of fungicides and pesticides, germplasm screening for host resistance has already begun. The resistant lines identified in the current study offers better choices to tackle anthracnose and could be used effectively in breeding programs to develop anthracnose resistant varieties.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73953169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Morpho-metric characterization and genetic variability studies in chrysanthemum 菊花形态计量学特征及遗传变异研究

IF 1.1 4区生物学

Plant Genetic Resources: Characterization and Utilization Pub Date : 2022-02-08 DOI: 10.1017/s1479262121000629

G. Kumar, Varun M. Hiremath, A. Tiwari, Vanlalruati, S. Sindhu

{"title":"Morpho-metric characterization and genetic variability studies in chrysanthemum","authors":"G. Kumar, Varun M. Hiremath, A. Tiwari, Vanlalruati, S. Sindhu","doi":"10.1017/s1479262121000629","DOIUrl":"https://doi.org/10.1017/s1479262121000629","url":null,"abstract":"\u0000 Germplasm characterization is essential to estimate variation and identify desirable genotypes for crop improvement. The current study was conducted on eight qualitative and 14 quantitative traits related to growth and floral attributes for characterization and grouping of 54 chrysanthemum genotypes. Wide variability was observed among most of the traits and principal component analysis revealed that the first two principal components explained most of the existing variation (>98%) in germplasm. Genotypic coefficient of variation exhibited a wide range from 9.41% (ratio of leaf length/width) to 105.99% (corolla tube length of ray florets), while phenotypic coefficient of variation varied from 10.49% (ratio of leaf length/width) to 106.38% (corolla tube length of ray florets). Broad sense heritability estimates were higher (>96%) for most of the characters, except for traits such as ratio of leaf length/width and peduncle thickness. Forty-four pair-wise inter-trait combinations showed positive genotypic and phenotypic correlations for 91 possible combinations. D2 analysis revealed that genotypes of clusters I and IV to be highly distinct, and hybridization between them might have better chance to obtain desirable types. D2 analysis confirmed that the cultivars in cluster IV namely, Red D Spoon, Tokyo Soldier, Yellow Reflex and hierarchical clustering showed that the cultivar Tokyo Soldier to be highly diverse. It is recommended to use the above genotypes in crossing programme for obtaining better progenies.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78412932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In-vivo haploid induction potential of Himalayan maize (Zea mays) and cogon grass (Imperata cylindrica) gene pools in different segregational cycles of intra and inter-generic crosses of wheat 小麦属内和属间杂交不同分离周期喜马拉雅玉米(Zea mays)和白茅(Imperata ica)基因库的体内单倍体诱导电位

IF 1.1 4区生物学

Plant Genetic Resources: Characterization and Utilization Pub Date : 2022-01-24 DOI: 10.1017/s1479262121000642

C. Kapoor, H. Chaudhary, P. Sharma, Ashima Relan, N. Manoj, Kritika Singh, V. K. Sood

{"title":"In-vivo haploid induction potential of Himalayan maize (Zea mays) and cogon grass (Imperata cylindrica) gene pools in different segregational cycles of intra and inter-generic crosses of wheat","authors":"C. Kapoor, H. Chaudhary, P. Sharma, Ashima Relan, N. Manoj, Kritika Singh, V. K. Sood","doi":"10.1017/s1479262121000642","DOIUrl":"https://doi.org/10.1017/s1479262121000642","url":null,"abstract":"\u0000 Maize and Imperata cylindrica have been utilized globally as a pollen source for induction of haploids in wheat through chromosome elimination technique. Pollen parents with a higher haploid induction rate are desired for recovering the high frequency of haploids in wheat and related species. The present investigation was carried out with the aim to assess haploid induction efficiency of diverse germplasm of maize and I. cylindrica in different generations of intra and intergeneric crosses of hexaploid and tetraploid wheat and triticale-wheat derivatives. Crosses of twenty-six lines (female) with each of two I. cylindrica and twenty-one maize genotypes (testers) were evaluated for four haploid induction parameters viz., pseudoseed formation frequency (PFF), embryo formation frequency (EFF), haploid regeneration frequency (HRF) and haploid formation frequency (HFF). I. cylindrica outperformed maize in haploid induction rate with a frequency of embryos formed with I. cylindrica (18.39%) were significantly higher as compared to maize (4.08%). In the case of I. cylindrica genotype Ic-ye identified best with mean EFF of 30.55, 14.48 and 25.43% for hexaploids, tetraploids and triticale × wheat derivatives, respectively whereas in the case of maize genotype HPMC-60 performed best with EFF of 12.61% for hexaploids, HPMC-58 (12.58%) for tetraploids and HPMC-16 for triticale × wheat derivatives with EFF of 8.91%. I. cylindrica genotype Ic-ye and maize genotypes HPMC-14, HPMC-53, HPMC-60, HPMC-64 with significantly positive GCA for haploid induction parameters may be utilized as efficient pollen parents for recovering higher frequency of haploids in wheat.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84442382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genetic combining, heterosis analysis for horticultural traits in tomato (Solanum lycopersicum L.) using ToLCV-resistant lines and molecular validation of Ty genes 番茄(Solanum lycopersicum L.)抗tolcv株系遗传组合、园艺性状杂种优势分析及Ty基因的分子验证

IF 1.1 4区生物学

Plant Genetic Resources: Characterization and Utilization Pub Date : 2022-01-07 DOI: 10.1017/s1479262121000630

R. Chandel, A. Sadashiva, K. Ravishankar, A. Das, B. M. Rout, Saurabh Singh

{"title":"Genetic combining, heterosis analysis for horticultural traits in tomato (Solanum lycopersicum L.) using ToLCV-resistant lines and molecular validation of Ty genes","authors":"R. Chandel, A. Sadashiva, K. Ravishankar, A. Das, B. M. Rout, Saurabh Singh","doi":"10.1017/s1479262121000630","DOIUrl":"https://doi.org/10.1017/s1479262121000630","url":null,"abstract":"\u0000 Tomato is a pivotal vegetable crop worldwide concerning human nutrition, economy and in service to biotechnology. Tomato leaf curl virus (ToLCV) is a begomovirus transmitted through the whitefly (Bemisia tabaci) and is responsible for severe losses in tomato production. In this context, the current investigation was carried out to determine heterotic combiners with high yield, resistance to ToLCV and mode of gene action for economically important traits. For this, 11 prescreened inbred lines were crossed in a half diallel fashion to generate 55 F1 hybrids. The 55 crosses with 11 parents and commercial checks were evaluated for different horticultural traits and resistance to ToLCV. The molecular validation with SCAR markers TG0302 and SCAR1 confirmed the presence of ToLCV-resistant genes in parents and their crosses. The hybrid IIHR-2902 × IIHR-2852 showed the presence of both Ty-2 and Ty-3 alleles in the homozygous state. The estimation of σSCA2 and σGCA2 was significant. It also indicated that the genetic control of target traits was under additive and non-additive gene effects. The values of σA/D2 along with σGCA2/σSCA2 found to be less than unity indicates the preponderance of non-additive gene action in the expression of the studied traits except for percent disease incidence. The parental line IIHR-2919 was the best combiner for fruit and yield traits. The cross combinations IIHR-2913 × IIHR-2898 exhibited significantly higher economic heterosis for yield along with the presence of Ty-2 and Ty-3 genes. The study paves the way for breeding high yielding and ToLCV-resistant hybrids in tomato.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72926361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3