Evaluation of resistant genotypes and their characterization using molecular markers linked for powdery mildew resistance in cucumber (Cucumis sativus L.)

IF 1.2 4区生物学 Q3 PLANT SCIENCES

Plant Genetic Resources: Characterization and Utilization Pub Date : 2022-01-07 DOI:10.1017/s1479262121000605

S. Sharma, A. Dar, Sakhsam Gupta, Ravinder Singh

引用次数: 0

Abstract

Powdery mildew (PM) is one of the most severe fungal diseases of cucumber that limits its production worldwide. In this study, 140 genotypes of cucumber were screened for disease resistance under field and artificial conditions, and then validated with eight known SSR markers linked to PM resistance. Among these genotypes, genotype GS140 was found resistant (R), whereas GS148, GS16 and GS70 were moderately resistant, and GS169 was found to be tolerant. Of all the eight markers, only C31, C80, C162, SSR16472 and SSR16881 amplified the reported linked allele. The 127 bp allele of SSR16881 was found to be associated with the lowest disease severity of 37.65%. The associated markers could further be verified for their usability using linkage studies and the contrast genotypes in the present study could serve as a tool for selection in future marker-assisted selection breeding strategies for PM resistance.

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黄瓜(Cucumis sativus L.)抗白粉病基因型评价及分子标记鉴定

白粉病是黄瓜最严重的真菌病害之一，严重制约了黄瓜的生产。本研究对140个黄瓜基因型进行了田间和人工条件下的抗病筛选，并利用8个已知的抗PM相关SSR标记进行了验证。在这些基因型中，基因型GS140为耐药(R)，基因型GS148、GS16和GS70为中等耐药，基因型GS169为耐药。在所有8个标记中，只有C31、C80、C162、SSR16472和SSR16881扩增了报道的连锁等位基因。SSR16881的127 bp等位基因与疾病严重程度最低的相关性为37.65%。相关标记可以通过连锁研究进一步验证其可用性，本研究的对比基因型可以作为未来标记辅助选择育种策略的选择工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Genetic Resources: Characterization and Utilization Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

2.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Plant Genetic Resources is an international journal which provides a forum for describing the application of novel genomic technologies, as well as their integration with established techniques, towards the understanding of the genetic variation captured in both in situ and ex situ collections of crop and non-crop plants; and for the airing of wider issues relevant to plant germplasm conservation and utilisation. We particularly welcome multi-disciplinary approaches that incorporate both a technical and a socio-economic focus. Technical aspects can cover developments in technologies of potential or demonstrated relevance to the analysis of variation and diversity at the phenotypic and genotypic levels.