Carlos E. González‐Orozco, J. A. Osorio-Guarín, R. Yockteng
{"title":"Phylogenetic diversity of cacao (Theobroma cacao L.) genotypes in Colombia","authors":"Carlos E. González‐Orozco, J. A. Osorio-Guarín, R. Yockteng","doi":"10.1017/s1479262123000047","DOIUrl":"https://doi.org/10.1017/s1479262123000047","url":null,"abstract":"\u0000 Theobroma cacao L. (cacao) is an important tropical crop used to produce chocolate. Evolutionary relationships between cultivated and wild cacao genotypes and their genetic diversity are poorly understood. Exploring phylogenetic diversity and spatial patterns of both cultivated and crop wild relatives can improve the knowledge of the evolutionary history of a crop, giving insights into its cultivation, breeding programmes and conservation. This study identifies biodiversity priority areas in Colombia by calculating phylogenetic diversity indices using a set of 87 single nucleotide polymorphism markers. These were sourced from 279 genotypes conserved in the Corporación Colombiana de Investigación Agropecuaria (Agrosavia) germplasm collection. The Caribbean and North Andes areas exhibited the highest phylogenetic diversity and significantly high relative phylogenetic diversity. We propose that those regions where wild cacao occurs should be prioritized as conservation areas. Besides, cacao lineages that have recently diverged and are present in Arauca, Huila and Nariño areas, with significantly low relative phylogenetic diversity, should be prioritized for breeding programmes. The Amazonia genotypes were closer to the root of the phylogenetic tree, suggesting an older origin than those found in the Andes region. Our study highlights the importance of using T. cacao germplasm from the Amazonia region as a priority to recover relict diversity in breeding programmes and broaden the gene pool of modern cultivated cacao.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":"78 1","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78951501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Syed Zain Kashif, M. Aslam, Zaheer Ahmed, F. Saleem, F. Alghabari, H. Alsamadany, H. A. Alzahrani, F. S. Awan
{"title":"Development and genomic characterization of EMS induced mutant population of Zea mays L.","authors":"Syed Zain Kashif, M. Aslam, Zaheer Ahmed, F. Saleem, F. Alghabari, H. Alsamadany, H. A. Alzahrani, F. S. Awan","doi":"10.1017/s1479262123000023","DOIUrl":"https://doi.org/10.1017/s1479262123000023","url":null,"abstract":"\u0000 Maize is among major field crops which provides food, fodder and various byproducts to the industry. Development of better performing varieties is very important to enhance and strengthen the maize production system. In this study ethyl methanesulfonate (EMS) is used to induce genetic variation in maize. Mutant population was derived from two genotypes 100,003 and 100,004. EMS was applied under three different concentrations of 25, 50 and 75 mM. 25 mM was found as an ideal concentration resulting in maximum survival rate. Total 10 SSRs were used in this study, which amplified 28 alleles with average of 2.7 alleles. Analysis of molecular variance showed significant differences present among individuals. Average heterozygosity for mutants derived from 100,003 and 100,004 was 0.58 and 0.53, respectively. UPGMA analysis characterized the mutants into two main and many sub clusters. According to the principal component analysis, PC 1 and 2 contributed to 64.2% variability with eigenvalue greater than 1. Statistics showed maximum coefficients of variance in traits of leaf area, cobb height and plant height. Promising mutants were also identified and recommended for future breeding programme. In conclusion, EMS mutagenesis is an effective technique to develop novel mutants that can be exploited in future breeding programmes.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":"283 1","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85449647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecular characterization of Brassica genebank germplasm confirms taxonomic identity and reveals low levels and source of taxonomic errors","authors":"Erica M. Steadman, K. Whitehouse, S. Norton","doi":"10.1017/s1479262123000035","DOIUrl":"https://doi.org/10.1017/s1479262123000035","url":null,"abstract":"\u0000 Crop germplasm conserved in genebanks, are a fundamental resource of genetic diversity for crop improvement activities, underpinning future food security and sustainable agricultural practices. However, taxonomic errors in genebank germplasm (due to misclassification, contamination and poor data collation) restrict the effective use of this material for correct purpose. Earlier studies investigating species genetic diversity using genebank germplasm, have shown varying levels of taxonomic error within the Brassica species. In response to this reported taxonomic error of global collections, together with the availability of a multiplex PCR (MPCR) marker, targeting the specific chromosomes (A, B and C) of the six Brassica species in U's triangle, this study was undertaken to confirm the taxonomic identity of accessions within the Australian Grains Genebank's (AGG) long-term Brassica collection. A total of 5161 accessions were analysed with MPCR for taxonomic identification, of which, 4842 (93.8%) were confirmed to be consistent (correct) with their labelled taxonomy, while the remaining 319 (6.2%) were identified as taxonomically inconsistent (in-error). Through the evaluation of earlier regeneration and original seed of the error accessions with MPCR, we determined that 80.9% of the taxonomic errors were traced back to the original seed, while 19.1% of errors were the result of genebank seed regeneration handling practices. Results from this study directly enhance information of the AGG Brassica collection and shape directions for distribution, acquisition and regeneration practices within the AGG and potentially other global genebanks, which will facilitate in a more effective use of these valuable genetic resources by researchers and breeders.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":"18 1","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83597436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Humara Fayaz, A. Wani, Asma Hamid Mir, Raheel Shafeeq Khan, F. Jan, M. Yasin, M. A. Bhat, R R Mir
{"title":"Trait variations for seed physical characteristics in chickpea (Cicer arietinum L.) from the Western Himalayas","authors":"Humara Fayaz, A. Wani, Asma Hamid Mir, Raheel Shafeeq Khan, F. Jan, M. Yasin, M. A. Bhat, R R Mir","doi":"10.1017/s1479262123000084","DOIUrl":"https://doi.org/10.1017/s1479262123000084","url":null,"abstract":"\u0000 Chickpea (Cicer arietinum L.) is one of the most important grain legumes after common bean. In the present study, a total of 35 chickpea genotypes including desi and Kabuli types of cultivated chickpea (C. arietinum) were evaluated for seed physical characteristics that are very important for seed storage, processing and determining seed quality traits. Therefore, it has become imperative to study the seed physical characteristics of chickpea during the present study. The analysis of data revealed that substantial variation is present among the genotypes under study with respect to seed physical characteristics such as seed size, seed volume, seed density, hydration capacity, hydration index, swelling capacity and swelling index. These traits are considered important to determine the seed rate at the time of sowing of the crop, cooking quality, storage and processing and thereby have a direct bearing on the economics of chickpea consumption. Our results indicated that the desi, Kabuli and pea-shaped genotypes differ significantly in seed physical characteristics with overall superior trait performance of the Kabuli type compared to other seed types, making them superior with respect to consumer/market demands.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":"39 1","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80172516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sabreena Bhat, Muslima Nazir, S. Zargar, Samiullah Naik, W. A. Dar, Bilal A. Bhat, Reetika Mahajan, B. Ganai, P. Sofi, S. Zargar
{"title":"In-depth morphological assessment revealed significant genetic variability in common buckwheat (Fagopyrum esculentum) and tartary buckwheat (Fagopyrum tataricum) germplasm","authors":"Sabreena Bhat, Muslima Nazir, S. Zargar, Samiullah Naik, W. A. Dar, Bilal A. Bhat, Reetika Mahajan, B. Ganai, P. Sofi, S. Zargar","doi":"10.1017/S1479262123000321","DOIUrl":"https://doi.org/10.1017/S1479262123000321","url":null,"abstract":"Abstract Buckwheat (Fagopyrum spp.) is an important crop in the high-altitude regions of the Northwest Indian Himalayas. The agro-climatic heterogeneity of this region offers a great deal of diversity in the agro-morphology of buckwheat species. In this study, a total of 61 accessions of Fagopyrum esculentum and Fagopyrum tataricum were characterized for 17 morphological (8 qualitative and 9 quantitative) traits. Significant differences (P < 0.0001) among all the traits were revealed by one-way analysis of variance. Further, significant phenotypic variability in both qualitative as well as quantitative traits was also observed. Both positive and negative correlations were observed between the traits of agronomic relevance. The principal component analysis (PCA) reveals about 69% variability among the first six components. The accessions were divided into two key clusters with numerous subclusters by considering the unweighted pair group method with arithmetic mean dendrogram. A cluster of 19 accessions was formed utilizing a PCA scatter plot indicating accessions with maximum values for important quality traits like plant height, leaf blade width, stem colour (red), primary branches, inflorescence length, flower colour (greenish-yellow), seed anthocyanin colour (green), seed shape (ovate) and seed weight. These accessions can be of vital significance for future buckwheat breeding programmes. The findings from the current study will form a favourable base for genetic resource management, improved cultivation and applications of buckwheat at the commercial level in the northwestern Himalayas of India.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":"22 1","pages":"417 - 424"},"PeriodicalIF":1.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75367978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oluwadurotimi S. Aworunse, J. Popoola, R. Paliwal, O. O. Obembe
{"title":"Start codon-targeted marker evaluation of genetic relationship and population structure in southern Nigerian fluted pumpkin (Telfairia occidentalis Hook F.) collection","authors":"Oluwadurotimi S. Aworunse, J. Popoola, R. Paliwal, O. O. Obembe","doi":"10.1017/S1479262123000308","DOIUrl":"https://doi.org/10.1017/S1479262123000308","url":null,"abstract":"Abstract Fluted pumpkin (Telfairia occidentalis Hook F.) is an underutilized indigenous leafy vegetable with enormous prospects for food security in sub-Saharan Africa. However, relatively little is known about genetic relationships and population structure in the species. In this study, 32 landraces of fluted pumpkin collected across three southern geographical regions in Nigeria were assessed for genetic diversity and population structure using 8 start codon-targeted (SCoT) makers. The polymorphic information content of the SCoT markers ranged from 0.48 in SCoT36 to 0.94 in SCoT28, with an average of 0.77. Hierarchical cluster dendrogram based on Ward's method and principal component analysis grouped the landraces into four clusters without affiliation to provenance. Overall, the mean values of the population genetic diversity parameters – Nei's gene diversity (H) and Shannon's information index (I) showed values of 0.28 ± 0.01 and 0.43 ± 0.02, respectively, implying a narrow genetic base for the landraces. The result was further corroborated by a very close Nei's genetic distance and identity among populations of the landraces. Furthermore, the south-west population exhibited the higher genetic diversity (H = 0.31 ± 0.02 and I = 0.45 ± 0.03). Population structure analysis inferred three subpopulations for the accessions with varying degrees of allelic admixture. An analysis of molecular variance revealed that almost all the genetic variation occurred within (99%) than between (1%) populations. The findings shed light on the genetic diversity of southern Nigerian fluted pumpkin and have significant implications for the characterisation, conservation, exploitation and improvement of the species.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":"68 1","pages":"406 - 416"},"PeriodicalIF":1.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78258174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xujuan Li, J. Mao, Xuekuan Chen, Xiuqin Lin, Xiao‐Yu Wang, Chaohua Xu, Xin-Long Liu, Xin Lu
{"title":"Molecular cytology identification of 22 sugarcane germplasm clones from Sri Lanka","authors":"Xujuan Li, J. Mao, Xuekuan Chen, Xiuqin Lin, Xiao‐Yu Wang, Chaohua Xu, Xin-Long Liu, Xin Lu","doi":"10.1017/S1479262123000278","DOIUrl":"https://doi.org/10.1017/S1479262123000278","url":null,"abstract":"Abstract Germplasm innovation can provide materials for breeding sugarcane cultivars. Saccharum officinarum is the main source of high-sugar and high-yield genes in sugarcane breeding. ‘Nobilization’ is the theoretical basis for exploiting S. officinarum, and S. officinarum authenticity directly affects sugarcane nobility breeding efficiency. Herein, the authenticity of 22 SLC-series S. officinarum clones imported from Sri Lanka and preserved in the China National Germplasm Repository of Sugarcane (NGRS) was explored by four-primer amplification-arrested mutation PCR (ARMS PCR) and somatic chromosome number counting. The amplified bands from SLC 08 120 and SLC 08 131 were the same with those from S. officinarum clone Badila, i.e. a common band of 428 bp and a S. officinarum-specific band of 278 bp, hence they were tentatively assigned as S. officinarum clones. The other 20 SLC clones had both 278 bp (S. officinarum-specific) and 203 bp (S. spontaneum-specific) bands, which are hybrid characteristics. In addition, the chromosome numbers of SLC 08 120 and SLC 08 131 are both 80, belong to typical S. officinarum. While the chromosome numbers of the other 20 materials are ranging from 101 to 129, consistent with hybrids of S. officinarum and S. spontaneum. This molecular cytological characterization indicates that among the 22 introduced SLC-series clones, only two, SLC 08 120 and SLC 08 131, were S. officinarum. Future agronomic trait and resistance analyses could facilitate their use as crossing parents in sugarcane breeding.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":"43 1","pages":"377 - 382"},"PeriodicalIF":1.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89426213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hamama Guetteche, Ali Jarrar, Ines Khiyel, Narimane Djekkoun, Leila Rouabah, A. Rouabah, A. Benbelkacem, P. Nick
{"title":"The popular Algerian barley landraces Saïda and Tichedrett are autochthonous – evidence from RAPD, SSR and agrophenological markers","authors":"Hamama Guetteche, Ali Jarrar, Ines Khiyel, Narimane Djekkoun, Leila Rouabah, A. Rouabah, A. Benbelkacem, P. Nick","doi":"10.1017/S1479262123000291","DOIUrl":"https://doi.org/10.1017/S1479262123000291","url":null,"abstract":"Abstract The characterisation of traditional barley varieties from North Africa is promising with respect to detect traits linked with resilience to drought. Nine Algerian barley varieties comprising the economically relevant local landraces Saïda183 and Tichedrett were genotyped by random amplifying polymorphic DNA (RAPD) and by Simple Sequence Repeat (SSR) markers to get insight into their phylogenetic relationship and to compare the efficiency of the two approaches. The SSR markers were chosen such that all chromosomes of barley were represented by at least one marker. In addition, a principal component analysis (PCA) of morpho-agronomical traits was conducted. We found that the phylogeny based on the RAPD markers reflected the geographical distribution of the tested varieties, while the phylogeny based on the SSR markers reflected the pedigree. Among the RAPD markers, the maximal polymorphism information content was obtained for BY14 with a value of 0.82, for the SSR markers, Bmg13 was most informative with a value of 0.60. The similarity matrix used to the construct the phylogenetic tree, inferred a close relationship (Jaccard Index 0.8 based on RAPD markers) between the accessions Fouara and Oued-Athmania occurring in close vicinity. The SSR markers group the autochthonous varieties Saïda183 and Tichedrett together with a Jaccard Index of 0.8. The close phylogenetic relationship between Saïda183 and Tichedrett is also supported by the PCA of morphological and agronomic traits. Our data underpin the diversity of Algerian barley varieties and lend support to the autochthonous origin of these two Algerian landraces.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":"57 1","pages":"394 - 405"},"PeriodicalIF":1.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80823954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Tsivelikas, E. Avramidou, P. Ralli, Ioannis Ganopoulos, Theodoros Moysiadis, A. Kapazoglou, F. Aravanopoulos, A. Doulis
{"title":"Genetic diversity of Greek grapevine (Vitis vinifera L.) cultivars using ampelographic and microsatellite markers","authors":"A. Tsivelikas, E. Avramidou, P. Ralli, Ioannis Ganopoulos, Theodoros Moysiadis, A. Kapazoglou, F. Aravanopoulos, A. Doulis","doi":"10.1017/s147926212200020x","DOIUrl":"https://doi.org/10.1017/s147926212200020x","url":null,"abstract":"\u0000 Grapevine (Vitis vinifera L.) is a major worldwide crop of high economic importance, tightly interwoven with the traditions and the culture of many civilizations. The Greek vineyard is one of the oldest in the world composed of an ample number of highly diverse indigenous landraces. However, over the last decades the local cultivated grapevine germplasm has undergone a drastic reduction of diversity, due to the established market preferences for international varieties. In the current work a combined approach involving both, ampelographic traits and microsatellite markers has been undertaken, to study the genetic diversity within and among 96 grapevine genotypes belonging to 36 V. vinifera subsp. vinifera cultivars, predominantly representing autochthonous Greek landraces. Results revealed high genetic diversity for the Greek cultivars yielding a mean number of alleles per locus 14.69 and mean polymorphic information content 0.848. Hierarchical cluster analysis, employing both, ampelographic and microsatellite data, showed a clear distinction based on the origin of the germplasm; Anatolian versus Mediterranean. Principal component analysis, based on the most informative ampelographic traits, coupled to the results from genetic structure analysis further corroborated the proposal of germplasm differentiation on the basis of geographic origin. This information can be further utilized for the reconstitution of the Greek vineyard and can significantly contribute towards a rational conservation and utilization strategy for breeding or for direct cultivation of the Greek indigenous grapevine germplasm.","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":"5 1","pages":""},"PeriodicalIF":1.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81833133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Reviewers’ List January 2022 to December 2022","authors":"","doi":"10.1017/s1479262123000448","DOIUrl":"https://doi.org/10.1017/s1479262123000448","url":null,"abstract":"","PeriodicalId":20252,"journal":{"name":"Plant Genetic Resources: Characterization and Utilization","volume":"104 1","pages":"442 - 442"},"PeriodicalIF":1.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76207896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}