PLoS Biology最新文献_第3页

It is time to authenticate the Microbiome Sciences with accredited educational programs and departments 现在是时候通过经认可的教育计划和部门对微生物组科学进行认证了

IF 9.8 1区生物学

PLoS Biology Pub Date : 2023-12-01 DOI: 10.1371/journal.pbio.3002420

Nichole Ginnan, Seth R. Bordenstein

引用次数: 0

Towards detailed predictions of coastal ecosystem function under climate change 努力详细预测气候变化下的沿岸生态系统功能

IF 9.8 1区生物学

PLoS Biology Pub Date : 2023-12-01 DOI: 10.1371/journal.pbio.3002430

Diego R. Barneche, Renato A. Morais

引用次数: 0

Editorial Note: The Communication Factor EDF and the Toxin-Antitoxin Module mazEF Determine the Mode of Action of Antibiotics. 编者按：通讯因子EDF和毒素抗毒素模块mazEF决定了抗生素的作用模式。

IF 7.8 1区生物学

PLoS Biology Pub Date : 2023-09-18 eCollection Date: 2023-09-01 DOI: 10.1371/journal.pbio.3002325

引用次数: 0

SEC14-like condensate phase transitions at plasma membranes regulate root growth in Arabidopsis. 质膜上类似SEC14的缩合物相变调节拟南芥根系的生长。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2023-09-18 eCollection Date: 2023-09-01 DOI: 10.1371/journal.pbio.3002305

Chen Liu, Andriani Mentzelopoulou, Fotini Papagavriil, Prashanth Ramachandran, Artemis Perraki, Lucas Claus, Sebastian Barg, Peter Dörmann, Yvon Jaillais, Philipp Johnen, Eugenia Russinova, Electra Gizeli, Gabriel Schaaf, Panagiotis Nikolaou Moschou

{"title":"SEC14-like condensate phase transitions at plasma membranes regulate root growth in Arabidopsis.","authors":"Chen Liu, Andriani Mentzelopoulou, Fotini Papagavriil, Prashanth Ramachandran, Artemis Perraki, Lucas Claus, Sebastian Barg, Peter Dörmann, Yvon Jaillais, Philipp Johnen, Eugenia Russinova, Electra Gizeli, Gabriel Schaaf, Panagiotis Nikolaou Moschou","doi":"10.1371/journal.pbio.3002305","DOIUrl":"10.1371/journal.pbio.3002305","url":null,"abstract":"Protein function can be modulated by phase transitions in their material properties, which can range from liquid- to solid-like; yet, the mechanisms that drive these transitions and whether they are important for physiology are still unknown. In the model plant Arabidopsis, we show that developmental robustness is reinforced by phase transitions of the plasma membrane-bound lipid-binding protein SEC14-like. Using imaging, genetics, and in vitro reconstitution experiments, we show that SEC14-like undergoes liquid-like phase separation in the root stem cells. Outside the stem cell niche, SEC14-like associates with the caspase-like protease separase and conserved microtubule motors at unique polar plasma membrane interfaces. In these interfaces, SEC14-like undergoes processing by separase, which promotes its liquid-to-solid transition. This transition is important for root development, as lines expressing an uncleavable SEC14-like variant or mutants of separase and associated microtubule motors show similar developmental phenotypes. Furthermore, the processed and solidified but not the liquid form of SEC14-like interacts with and regulates the polarity of the auxin efflux carrier PINFORMED2. This work demonstrates that robust development can involve liquid-to-solid transitions mediated by proteolysis at unique plasma membrane interfaces.","PeriodicalId":20240,"journal":{"name":"PLoS Biology","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10307574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective retention of dysfunctional mitochondria during asymmetric cell division in yeast. 酵母不对称细胞分裂过程中功能失调线粒体的选择性保留。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2023-09-18 eCollection Date: 2023-09-01 DOI: 10.1371/journal.pbio.3002310

Xenia Chelius, Veronika Bartosch, Nathalie Rausch, Magdalena Haubner, Jana Schramm, Ralf J Braun, Till Klecker, Benedikt Westermann

{"title":"Selective retention of dysfunctional mitochondria during asymmetric cell division in yeast.","authors":"Xenia Chelius, Veronika Bartosch, Nathalie Rausch, Magdalena Haubner, Jana Schramm, Ralf J Braun, Till Klecker, Benedikt Westermann","doi":"10.1371/journal.pbio.3002310","DOIUrl":"10.1371/journal.pbio.3002310","url":null,"abstract":"Decline of mitochondrial function is a hallmark of cellular aging. To counteract this process, some cells inherit mitochondria asymmetrically to rejuvenate daughter cells. The molecular mechanisms that control this process are poorly understood. Here, we made use of matrix-targeted D-amino acid oxidase (Su9-DAO) to selectively trigger oxidative damage in yeast mitochondria. We observed that dysfunctional mitochondria become fusion-incompetent and immotile. Lack of bud-directed movements is caused by defective recruitment of the myosin motor, Myo2. Intriguingly, intact mitochondria that are present in the same cell continue to move into the bud, establishing that quality control occurs directly at the level of the organelle in the mother. The selection of healthy organelles for inheritance no longer works in the absence of the mitochondrial Myo2 adapter protein Mmr1. Together, our data suggest a mechanism in which the combination of blocked fusion and loss of motor protein ensures that damaged mitochondria are retained in the mother cell to ensure rejuvenation of the bud.","PeriodicalId":20240,"journal":{"name":"PLoS Biology","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10310579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asymmetric connections with starburst amacrine cells underlie the upward motion selectivity of J-type retinal ganglion cells. 与星突无长突细胞的不对称连接是J型视网膜神经节细胞向上运动选择性的基础。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2023-09-18 eCollection Date: 2023-09-01 DOI: 10.1371/journal.pbio.3002301

Bo Wang, Yifeng Zhang

{"title":"Asymmetric connections with starburst amacrine cells underlie the upward motion selectivity of J-type retinal ganglion cells.","authors":"Bo Wang, Yifeng Zhang","doi":"10.1371/journal.pbio.3002301","DOIUrl":"10.1371/journal.pbio.3002301","url":null,"abstract":"Motion is an important aspect of visual information. The directions of visual motion are encoded in the retina by direction-selective ganglion cells (DSGCs). ON-OFF DSGCs and ON DSGCs co-stratify with starburst amacrine cells (SACs) in the inner plexiform layer and depend on SACs for their direction selectivity. J-type retinal ganglion cells (J-RGCs), a type of OFF DSGCs in the mouse retina, on the other hand, do not co-stratify with SACs, and how direction selectivity in J-RGCs emerges has not been understood. Here, we report that both the excitatory and inhibitory synaptic inputs to J-RGCs are direction-selective (DS), with the inhibitory inputs playing a more important role for direction selectivity. The DS inhibitory inputs come from SACs, and the functional connections between J-RGCs and SACs are spatially asymmetric. Thus, J-RGCs and SACs form functionally important synaptic contacts even though their dendritic arbors show little overlap. These findings underscore the need to look beyond the neurons' stratification patterns in retinal circuit studies. Our results also highlight the critical role of SACs for retinal direction selectivity.","PeriodicalId":20240,"journal":{"name":"PLoS Biology","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10300937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A TOX-ic axis of epigenetic stem cell maintenance and chemoresistance in colon cancer. 癌症表观遗传干细胞维持和化疗耐药性的TOX-ic轴。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2023-09-15 eCollection Date: 2023-09-01 DOI: 10.1371/journal.pbio.3002295

Christopher G Hubert, Shaun R Stauffer, Justin D Lathia

引用次数: 0

azyx-1 is a new gene that overlaps with zyxin and affects its translation in C. elegans, impacting muscular integrity and locomotion. azyx-1是一个与zyxin重叠的新基因，影响其在秀丽隐杆线虫中的翻译，影响肌肉的完整性和运动。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2023-09-15 eCollection Date: 2023-09-01 DOI: 10.1371/journal.pbio.3002300

Bhavesh S Parmar, Amanda Kieswetter, Ellen Geens, Elke Vandewyer, Christina Ludwig, Liesbet Temmerman

引用次数: 0

A new polymodal gating model of the proton-activated chloride channel. 一种新的质子激活氯化物通道的多模式门控模型。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2023-09-15 eCollection Date: 2023-09-01 DOI: 10.1371/journal.pbio.3002309

Piao Zhao, Cheng Tang, Yuqin Yang, Zhen Xiao, Samantha Perez-Miller, Heng Zhang, Guoqing Luo, Hao Liu, Yaqi Li, Qingyi Liao, Fan Yang, Hao Dong, Rajesh Khanna, Zhonghua Liu

{"title":"A new polymodal gating model of the proton-activated chloride channel.","authors":"Piao Zhao, Cheng Tang, Yuqin Yang, Zhen Xiao, Samantha Perez-Miller, Heng Zhang, Guoqing Luo, Hao Liu, Yaqi Li, Qingyi Liao, Fan Yang, Hao Dong, Rajesh Khanna, Zhonghua Liu","doi":"10.1371/journal.pbio.3002309","DOIUrl":"10.1371/journal.pbio.3002309","url":null,"abstract":"The proton-activated chloride (PAC) channel plays critical roles in ischemic neuron death, but its activation mechanisms remain elusive. Here, we interrogated PAC channel gating using its unique bidirectional modulator C77304 as a pharmacological probe. C77304 activated the PAC channel by acting on its proton gating, while simultaneously inhibiting channel activity at higher doses, through interaction with two modulatory sites with different affinities and state-dependence. Excitingly, we revealed that PAC undergoes intrinsic proton gating-independent voltage activation, which was defined by an ion-flux gating mechanism. Scanning-mutagenesis and molecular dynamics simulation confirmed that E181, E257, and E261 in human PAC form the primary proton sensors, as alanine mutations eliminated the channel’s proton gating while sparing the voltage-dependent gating. This proton sensing mechanism was basically conserved among orthologous PAC channels. Collectively, our data unveils the polymodal gating and proton sensing mechanisms in the PAC channel which may inspire potential drug development.","PeriodicalId":20240,"journal":{"name":"PLoS Biology","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10529583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10265086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transient eco-evolutionary dynamics early in a phage epidemic have strong and lasting impact on the long-term evolution of bacterial defences. 噬菌体流行早期的短暂生态进化动力学对细菌防御的长期进化有着强烈而持久的影响。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2023-09-15 eCollection Date: 2023-09-01 DOI: 10.1371/journal.pbio.3002122

Bridget Nora Janice Watson, Elizabeth Pursey, Sylvain Gandon, Edze Rients Westra

{"title":"Transient eco-evolutionary dynamics early in a phage epidemic have strong and lasting impact on the long-term evolution of bacterial defences.","authors":"Bridget Nora Janice Watson, Elizabeth Pursey, Sylvain Gandon, Edze Rients Westra","doi":"10.1371/journal.pbio.3002122","DOIUrl":"10.1371/journal.pbio.3002122","url":null,"abstract":"Organisms have evolved a range of constitutive (always active) and inducible (elicited by parasites) defence mechanisms, but we have limited understanding of what drives the evolution of these orthogonal defence strategies. Bacteria and their phages offer a tractable system to study this: Bacteria can acquire constitutive resistance by mutation of the phage receptor (surface mutation, sm) or induced resistance through their CRISPR-Cas adaptive immune system. Using a combination of theory and experiments, we demonstrate that the mechanism that establishes first has a strong advantage because it weakens selection for the alternative resistance mechanism. As a consequence, ecological factors that alter the relative frequencies at which the different resistances are acquired have a strong and lasting impact: High growth conditions promote the evolution of sm resistance by increasing the influx of receptor mutation events during the early stages of the epidemic, whereas a high infection risk during this stage of the epidemic promotes the evolution of CRISPR immunity, since it fuels the (infection-dependent) acquisition of CRISPR immunity. This work highlights the strong and lasting impact of the transient evolutionary dynamics during the early stages of an epidemic on the long-term evolution of constitutive and induced defences, which may be leveraged to manipulate phage resistance evolution in clinical and applied settings.","PeriodicalId":20240,"journal":{"name":"PLoS Biology","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10263043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0