{"title":"Hairy roots as a potential source for the production of rosmarinic acid from genus Salvia","authors":"Somani Shriti, Agarwala Kuldeep Prasad, Wudali Narasimha Sudheer, Nagella Praveen","doi":"10.14719/pst.2541","DOIUrl":"https://doi.org/10.14719/pst.2541","url":null,"abstract":"The Salvia genus, a member of the Lamiaceae family, exhibits a rich array of secondary metabolites, including di- and triterpenoids, phenols, polyphenols, and essential oil compounds. These constituents contribute to valuable pharmacological activities such as antibacterial, antiviral, anti-inflammatory, and antioxidant properties. Among these metabolites, rosmarinic acid stands out as a particularly promising compound, deriving from the precursors phenylalanine and tyrosine. It belongs to the phenolic compound class and acts as an ester of caffeic acid, showcasing diverse therapeutic potentials like antifungal, antibacterial, antiviral, antioxidant, anticancer, anti-ageing, anti-inflammatory, and anti-diabetic effects. To facilitate the production of such secondary metabolites, plant tissue culture techniques have played a pivotal role, with hairy root cultures being one of the preferred methods. This review provides an extensive examination of the biosynthetic pathway of rosmarinic acid and its successful generation using hairy root cultures. Additionally, the review highlights the utilization of genetic modification tools and various biotic and abiotic elicitors, including yeast extract, methyl jasmonate, and silver ion (Ag+), in hairy root cultures of diverse Salvia species to enhance the production of rosmarinic acid.","PeriodicalId":20236,"journal":{"name":"Plant Science Today","volume":"16 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139246126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diana Jhulia Palheta de Sousa, Tamirys Marcelina da Silva, Marcio Augusto Costa Carmona Junior, Glauco André Dos Santos Nogueira, Ana Ecídia De Araújo Brito, Luma Castro de Souza, Cândido Ferreira de Oliveira Neto, Gerson Diego Pamplona Albuquerque
{"title":"Effect of salicylic acid on cowpea seedlings under saline stress","authors":"Diana Jhulia Palheta de Sousa, Tamirys Marcelina da Silva, Marcio Augusto Costa Carmona Junior, Glauco André Dos Santos Nogueira, Ana Ecídia De Araújo Brito, Luma Castro de Souza, Cândido Ferreira de Oliveira Neto, Gerson Diego Pamplona Albuquerque","doi":"10.14719/pst.2237","DOIUrl":"https://doi.org/10.14719/pst.2237","url":null,"abstract":"The aim of this work was applying salicylic acid (SA) in cowpea seedlings under saline stress. The experiment took place in the seed laboratory of the Universidade Federal Rural da Amazônia with a completely randomized experimental design in a 2 x 2 x 3 factorial scheme, with two bean cultivars (Canapu and Pingo-de-ouro), two levels of salicylic acid (0, and 0.50 mM) and three salt stress levels (0, 25, 50 mM). The seeds were previously soaked in salicylic acid (0 and 0.50 mM) for a period of 12 hours and then placed in germitest paper rolls for treatments with NaCl (0, 25, 50 mM) for a period of 12 days at room temperature constant 27 °C. There was a significant effect of cultivars, AS dose and NaCl concentrations and their interactions on most of the analyzed variables. Root and leaf proline concentrations were higher in pingo-de-ouro cultivar, Canapu cultivar had better performance in biomass accumulation. Salicylic acid reduced proteins in the leaves by 13.33%, while in the root there was an increase of 12.61%, ammonium concentrations reduced in the roots by 11.9%. When applied to salinity (25 and 50 mM) there was an increase of proteins in the leaves 40.83% and 27.48% respectively, and a reduction of amino acids of 30.24 and 25.24% in NaCl dosages (25 and 50 mM) respectively. Salinity reduced biomass accumulation and interfered with cellular solute production. However, the application of salicylic acid promoted salt stress tolerance in Canapu cultivar.","PeriodicalId":20236,"journal":{"name":"Plant Science Today","volume":"13 2","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139243470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The population structure of Nepeta pamirensis at different altitudes in the Pamirs (Tajikistan)","authors":"Alexey Yurievich Astashenkov, V.A. Cheryomushkina, Maryio Tilloevich Boboev","doi":"10.14719/pst.2630","DOIUrl":"https://doi.org/10.14719/pst.2630","url":null,"abstract":"The structure of alpine plant populations is one of the main criteria for assessing the current state of alpine flora. Species of the genus Nepeta, most of which belong to alpine plants, can be universal objects for assessing changes in environmental conditions, including the impact of anthropogenic pressure. The article discusses the ontogenetic structure and population size in connection with the change in the life form of Nepeta pamirensis at different heights of the Pamirs (3060-4250 m a.s.l.). Our research showed that the ontogenetic spectra of different populations are different. These differences are linked to changes in the life form, the length of ontogenesis and how well seeds reproduce. Populations were studied using transects. The individual seed was taken as the counting unit. In total, 3 populations were studied and more than 750 individuals of different ontogenetic states were included in the analysis. The ontogenetic structure of populations was characterized using basic demographic indicators: the recovery index, ageing index, generative index and the ecological density index. Depending on the altitude gradient, the species was characterized by different efficiencies of seed reproduction, which determined the different densities of individuals in plant communities of distribution. In general, with an increase in the height of distribution of a species, the structure of the population became more stable.","PeriodicalId":20236,"journal":{"name":"Plant Science Today","volume":"26 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139242859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Efficiency of some local isolates of arbuscular mycorrhizae in the growth and productivity of potatoes (Solanum tuberosum L.) in plastic pots","authors":"Nour Sabah Naji, Naeem Saeed Dhiyab, R. Abed","doi":"10.14719/pst.2923","DOIUrl":"https://doi.org/10.14719/pst.2923","url":null,"abstract":"The present study was conducted using plastic pots to investigate the efficiency of 15 local isolates of mycorrhizal fungi in enhancing the growth and productivity of Arizona variety potatoes (Solanum tuberosum L.). These isolates were obtained from various wild plants, including the Sweet rush plant, Sudan grass, and Millet, collected from different districts in Diyala Governorate. The isolates were obtained through the single spore cultivation technique in the Department of Agricultural Research in Al-Zafaraniya. The results demonstrated that all 15 fungal isolates had the ability to infect the roots of potato plants. Notably, M11 showed a significant superiority in infection severity (98.3%), the number of spores was 67.67 spores/gm of soil, and the infection rate was 90.0%. The results also highlighted the impact of these isolates on various parameters related to vegetative and root growth as well as yield. Specifically, isolates M1 and M12 were found to be superior in promoting plant height and increasing leaf area, which reached 218.3 cm2. Isolate M4 was superior in increasing the chlorophyll content of leaves, reaching a level of 47.4 spad. In the context of vegetative growth, isolate M3 produced a notable fresh weight of 8.236 g, while isolate M8 yielded a dry vegetative weight of 7.533 g. Regarding the root system, isolate M11 displayed superiority in root length, reaching 45.20 cm. Isolate M8 showed a higher number of tubers, amounting to 11.33 g, whereas isolate M7 produced tubers with a weight of 178.5 g.","PeriodicalId":20236,"journal":{"name":"Plant Science Today","volume":"111 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139244763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biofuels from cyanobacteria -a metabolic engineering approach","authors":"P Pooja, K Edison Lekshmi, Pradeep N S","doi":"10.14719/pst.2505","DOIUrl":"https://doi.org/10.14719/pst.2505","url":null,"abstract":"The concern about the limited availability of petroleum-based fuels and their role in increasing CO2 levels in the atmosphere has sparked significant attention toward biofuel and bioenergy production. The global pursuit of sustainable energy sources has catalyzed innovative research into alternative biofuel production strategies. Transforming CO2 into usable fuels and chemicals is gaining even more prominence. Cyanobacteria, renowned for their photosynthetic ability, have emerged as promising candidates for biofuel synthesis. Their ability to convert solar energy and carbon dioxide into valuable biofuels makes them a compelling avenue for sustainable energy solutions. Using metabolic engineering principles, researchers have endeavored to optimize cyanobacterial metabolic pathways, enhance photosynthetic efficiency, and redirect carbon flux toward biofuel precursors. Numerous species of cyanobacteria offer genetic and metabolic traits that facilitate manipulation, and their photosynthetic characteristics imply that carbohydrates, fatty acids, and even alcohol could serve as potential renewable sources for biofuels. This review showcases cyanobacteria's ability as a biofuel source and emphasizes the transformative influence of metabolic engineering employed in the creation and production of \"cyanofuels”","PeriodicalId":20236,"journal":{"name":"Plant Science Today","volume":"120 13","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135137576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Seed Vigor of Soybean Treated by Corona Discharge Plasma","authors":"Lianfeng Lin, Runing Liang, Xin Liu, Dan Zhang, Mingzhe Wang, Wenhao Zhao, Xiaojiang Tang, Baoxia Li, Guannan Shi, Wenzhuo Chen, Junwei Guo, Eric Robert, Feng Huang","doi":"10.14719/pst.2288","DOIUrl":"https://doi.org/10.14719/pst.2288","url":null,"abstract":"There is a huge gap between the output and demand of soybean in China. How to improve the seed vigor of soybean has always been a research focus. Low temperature plasma (LTP) is a new green technology, which is widely used in crop seed treatment. Corona plasma is a typical discharge mode of plasma, which can affect the vigor of seeds. The effect of different discharge power on the soybean seed vigor by plasma treatment was experimentally investigated. Plasma discharge characteristic wavelength and spatial distribution were analysed. It shows that the corona discharge spectrum mainly exhibits the strong ultraviolet radiation and 90% of the spectral intensity focused in the center of discharge region. Water absorption and germination index of seeds and the fresh weight of seedlings were used to characterize the specific effects caused by different plasma powers. The results show that plasma treatment has a significant effect on the early stage of germination and can significantly affect the soybean seed vigor and growth. Overdose treatment will cause inhibiting effect. This study provides an experimental basis for the practical agriculture application of corona plasma seed treatment.","PeriodicalId":20236,"journal":{"name":"Plant Science Today","volume":" 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135290997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Asrorov Akmal, Ayubov Mirzakamol, M Darmanov Mukhtor, Narmatov Sardor, Mamajanov Akramjon, Bozorov Ilhomjon, N Khusenov Naim, Matusikova Ildiko, T Buriev Zabardast, Y Abdurakhmonov Ibrokhim
{"title":"Chemical Interventions to Alleviate Salt Stress in Cotton Plants: A Review","authors":"M Asrorov Akmal, Ayubov Mirzakamol, M Darmanov Mukhtor, Narmatov Sardor, Mamajanov Akramjon, Bozorov Ilhomjon, N Khusenov Naim, Matusikova Ildiko, T Buriev Zabardast, Y Abdurakhmonov Ibrokhim","doi":"10.14719/pst.2522","DOIUrl":"https://doi.org/10.14719/pst.2522","url":null,"abstract":"The alleviation of salt stress in cotton plants through the application of exogenous chemicals has emerged as a viable strategy to mitigate the adverse effects on various plant attributes, including growth, development, yield, and flowering. Plant hormones, known for their efficacy at low doses, have garnered significant attention in this context. Despite being inherently susceptible to salt stress, cotton plants experience severe impediments in water absorption from the soil, leading to delayed growth and development. Several phytohormones, including jasmonic acid, salicylic acid, and glycine betaine, have been extensively investigated in numerous studies for their potential to ameliorate salt stress in cotton plants. Promising results have been obtained with both foliar and seed treatments employing these substances. This foundational knowledge has paved the way for the development of alternative strategies to mitigate salt stress. However, the practical utilization of these chemicals is hindered by their elevated cost. Plant growth regulators, such as nitric oxide and melatonin, have also garnered interest for their ability to alleviate salt stress in cotton plants. Numerous studies have corroborated their effectiveness in this regard. This review comprehensively examines the aforementioned substances and extracts that have been investigated for their potential to mitigate the detrimental effects of salt stress on cotton plants.","PeriodicalId":20236,"journal":{"name":"Plant Science Today","volume":" 25","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135292093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ignat Ignatov, Teodora P. Popova, Tanya Toshkova-Yotova, Veronika Deleva, Ralitsa Bankova, Nikolai Neshev, Mario T. Iliev, Toshka E. Petrova, Mila D. Kaleva, Christos Drossinakis, Alexander I. Ignatov, Zhechko Dimitrov, Irina Gotova, Fabio Huether, Mariana Angelcheva, Ivan Angushev, Nedyalka Valcheva, Stoil D. Karadzhov, Sadek Baiti
{"title":"Research on the structuring of water clusters in Chlorella vulgaris water suspension","authors":"Ignat Ignatov, Teodora P. Popova, Tanya Toshkova-Yotova, Veronika Deleva, Ralitsa Bankova, Nikolai Neshev, Mario T. Iliev, Toshka E. Petrova, Mila D. Kaleva, Christos Drossinakis, Alexander I. Ignatov, Zhechko Dimitrov, Irina Gotova, Fabio Huether, Mariana Angelcheva, Ivan Angushev, Nedyalka Valcheva, Stoil D. Karadzhov, Sadek Baiti","doi":"10.14719/pst.2493","DOIUrl":"https://doi.org/10.14719/pst.2493","url":null,"abstract":"Many bioactive compounds of natural origin have beneficial effects on human health and are used to treat different diseases. Chlorella is a genus of green algae with a high potential for producing biologically active substances. Exposure to extreme conditions can enhance its antioxidant activity and the production of concrete metabolites. C. vulgaris is cultivated in plantations. It is accessible in pharmacies and drugstores. The Health Act of 2005 in Bulgaria allows the therapeutic and prophylactic use of herbs, both independently by patients and as prescribed by a doctor. This study performed comparative spectral analyses of C. vulgaris using a 1% suspension of C. vulgaris in deionized water (v/v) by the methods of Non-equilibrium energy spectrum (NES) and Differential non-equilibrium energy spectrum (DNES). The research was performed in order to make indirect studies of the biological effects of C. vulgaris, which are connected with calcium conductivity and anti-inflammatory and anti-tumor effects. The effects of structuring of water clusters by C. vulgaris were examined. The data from spectral analyses, connected with a peak at (E =-0.1312 eV)(?=9.45 ?m) (?=1058 cm-1), revealed anti-inflammatory effects. The anti-oxidant and anti-tumor effects of C. vulgaris were shown at (E=-0.1387 eV)(?=8.95 ?m)(?=1117 cm-1). The results showed effects of improvement of calcium conductivity and anti-inflammatory, antioxidant and antitumor effects of C. vulgaris on human health.","PeriodicalId":20236,"journal":{"name":"Plant Science Today","volume":" 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135290607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C S Ramyashree, C Morris Carol, P Kruthika, Pappuswamy Manikantan, Chaudhary Aditi, Meyyazhagan Arun, Arumugam Vijaya Anand, Balasubramanian Balamuralikrishnan
{"title":"Role of Bacillus thuringiensis in development of transgenic plants","authors":"C S Ramyashree, C Morris Carol, P Kruthika, Pappuswamy Manikantan, Chaudhary Aditi, Meyyazhagan Arun, Arumugam Vijaya Anand, Balasubramanian Balamuralikrishnan","doi":"10.14719/pst.2370","DOIUrl":"https://doi.org/10.14719/pst.2370","url":null,"abstract":"One of the most significant advancements in plant biotechnology has been the production of genetically engineered plants. Due to the effects of pests damaging the majority of crops, the development of pest immunity was necessary for crop preservation. Plants that have had their gene makeup altered in-utero, such as Bacillus thuringiensis, which has insecticidal properties and helps protect crops from pests, are referred to as \"genetically modified plants.\" Cry proteins, which are poisonous proteins that exist in the state of crystals, are the major genes responsible for the development of transgenic plants. Based on the effect of different pest species, cry proteins are divided into many categories. Since they are extremely specific by nature and only affect the target proteins, they are considered environmentally beneficial pesticides since they have no impact on the physiologically significant soil bacteria or other bacterial flora. These cry proteins stay as dormant crystals, but when a pest consumes plants, the inactive form of the crystals becomes active in the alkaline stomach pH of the microorganism, aiding in the rupture of the gut epithelium and ultimately causing the microorganism to die. These days, transgenic plants have been created, including BT corn, BT rice, sugarcane, brinjal, potato, tomato, and many more, it was also discovered that using these transgenic plants increased crop productivity. Transgenic plants can prevent several ecological issues associated with traditional pesticides, including the emergence of resistance, their toxicity to non-target living things, and the buildup of toxic waste in the environment.","PeriodicalId":20236,"journal":{"name":"Plant Science Today","volume":" 10","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135292370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Drought stress tolerance in rice: a critical insight","authors":"Debapriya Choudhury, Chandrama Mukherjee, Shinjan Dey, Sikha Dutta","doi":"10.14719/pst.2613","DOIUrl":"https://doi.org/10.14719/pst.2613","url":null,"abstract":"Drought is currently a serious threat for farming especially in rice cultivation, due to its substantial water requirements throughout its lifecycle. Drought is one of the major environmental constraints disrupting the growth and yield of rice plants, affecting them at physiological, morphological, biochemical and molecular levels. Global climate change exacerbates this issue, leading to substantial economic losses. As rice is a major food crop worldwide, the demand for rice production is increasing in tandem with the expanding human population. Consequently, it has become imperative to utilize drought-prone areas for agriculture and develop drought-tolerant rice genotypes. In addition to conventional breeding methods, the application of multi-omics approaches proves most effective in meeting the need to enhance drought tolerance in rice plants. Protective mechanisms, such as morphological adaptation, physiological acclimatization, cellular adjustments and antioxidant defense, play pivotal roles in helping plants overcome drought stress. Plant-microbial interactions are important for plants to overcome drought-induced adversities. Furthermore, applications of conventional approaches, omics approaches and nanotechnology are very promising for generating climate smart agriculture. Our aim in this review is to focus on drought stress tolerance in rice including drought-tolerant rice genotypes, their adaptation mechanisms, the unveiling the genes, transcription factors, microRNAs (miRNA) involved, microbial assistance and exploring approaches to mitigate drought stress in rice plants. The present review might throw some light on understanding the mechanism of drought stress tolerance in rice, including its molecular crosstalk and biochemical dynamics, for future researchers.","PeriodicalId":20236,"journal":{"name":"Plant Science Today","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135725104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}