发布求助
文献互助 智能选刊 最新文献

Plant Science Letters最新文献

筛选
英文 中文
Phaseolus vulgaris lectin heterogeneity related to their metal content 菜豆凝集素异质性与金属含量的关系
Plant Science Letters Pub Date : 1984-11-01 DOI: 10.1016/0304-4211(84)90211-6
D. Manen, J.F. Manen, M.R. Morgan
{"title":"Phaseolus vulgaris lectin heterogeneity related to their metal content","authors":"D. Manen,&nbsp;J.F. Manen,&nbsp;M.R. Morgan","doi":"10.1016/0304-4211(84)90211-6","DOIUrl":"10.1016/0304-4211(84)90211-6","url":null,"abstract":"<div><p>The seed lectins of <em>Phaseolus vulgaris</em> cv. Contender typically consist of five tetrameric isolectins E<sub>4</sub>, E<sub>3</sub>L, E<sub>2</sub>L<sub>2</sub>, EL<sub>3</sub> and L<sub>4</sub>. However electrophoretic and isoelectric focusing analysis of each purified protomer show a great heterogeneity. We demonstrate that each tetrameric isolectin exists in five different electrophoretic forms depending on the amount of Mn<sup>2+</sup> cation bound to each isolectin. The effects of Mn<sup>2+</sup> binding on charge and/or molecular properties of the protomers are discussed. Under the same experimental conditions, if we use Ca<sup>2+</sup> ions instead of Mn<sup>2+</sup>, the abovemodifications are not observed.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90211-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76669400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Transition between seed reserve use and photosynthetic supply during development of maize seedlings 玉米幼苗发育过程中种子储备利用与光合供给的过渡
Plant Science Letters Pub Date : 1984-11-01 DOI: 10.1016/0304-4211(84)90199-8
E. Deleens , N. Gregory , R. Bourdu
{"title":"Transition between seed reserve use and photosynthetic supply during development of maize seedlings","authors":"E. Deleens ,&nbsp;N. Gregory ,&nbsp;R. Bourdu","doi":"10.1016/0304-4211(84)90199-8","DOIUrl":"10.1016/0304-4211(84)90199-8","url":null,"abstract":"<div><p>Using the carbon isotope ratio <span><math><msup><mi></mi><mn>13</mn></msup><mtext>C</mtext><msup><mi></mi><mn>12</mn></msup><mtext>C</mtext></math></span> we have determined the relative importance of carbon flow from the seed reserves and the carbon provided by leaf photosynthetic fixation during the course of development of maize seedlings. The transition from the heterotrophic stage to the autotrophic one corresponds to a growth crisis which occurs on the 10th day in our conditions. During the first 7 days after germination δ <sup>13</sup>C in leaves and roots is the same as that of the reserve tissues, then a rapid isotropic dilution by incorporation of photosynthetic carbon is observed. The balance between heterotropic and autotrophic carbon is obtained on the 10th day in the leaves but only on the 13th or 14th day in the roots. No autotropic carbon occurs in the roots before the 10th day. It seems that the growth crisis would be due or corresponds, at least partially, to the setting up of translocative function of products from the leaves to the roots.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90199-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77874873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 49
Purification of several NADP-dependent malate dehydrogenase isozymes from spinach leaves. Kinetic properties 菠菜叶片中几种nadp依赖性苹果酸脱氢酶同工酶的纯化。动态属性
Plant Science Letters Pub Date : 1984-11-01 DOI: 10.1016/0304-4211(84)90213-X
Nathalie Ferté, Jean-Claude Meunier
{"title":"Purification of several NADP-dependent malate dehydrogenase isozymes from spinach leaves. Kinetic properties","authors":"Nathalie Ferté,&nbsp;Jean-Claude Meunier","doi":"10.1016/0304-4211(84)90213-X","DOIUrl":"10.1016/0304-4211(84)90213-X","url":null,"abstract":"<div><p>Four NADP-malate dehydrogenase (NADP-MDH) (EC 1.1.1.82) isozymes from spinach leaves have been purified to apparent homogeneity by DEAE-cellulose and affinity chromatography, and molecular sieving. They have the same native molecular weight (≈57 000) as determined by sedimentation equilibration analysis. They can be distinguished by their eletrocphoretic and chromatographic (over DEAE-cellulose) behaviors. On this basis, they constitute two sets of two enzymes each: MDH-A and -B, and MDH-C and -D. Catalytic properties of the most abundant isozyme, MDH-A, have been studied. Both NADPH and oxaloacetate inhibit the forward reductive reaction. Other kinetic parameters are also presented.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90213-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77429327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Selection and characterization of NaCl tolerant cells from embryogenic cultures of Pennisetum purpureum schum. (Napier grass) 紫荆狼尾草胚性培养耐NaCl细胞的筛选与特性研究。(纳皮尔草)
Plant Science Letters Pub Date : 1984-11-01 DOI: 10.1016/0304-4211(84)90219-0
Stephen F. Chandler, Indra K. Vasil
{"title":"Selection and characterization of NaCl tolerant cells from embryogenic cultures of Pennisetum purpureum schum. (Napier grass)","authors":"Stephen F. Chandler,&nbsp;Indra K. Vasil","doi":"10.1016/0304-4211(84)90219-0","DOIUrl":"10.1016/0304-4211(84)90219-0","url":null,"abstract":"<div><p>Sodium chloride (salt) tolerant callus was selected from leaf-derived embryogenic callus of <em>Pennisetum purpureum</em>. The tolerant callus was obtained after repeated subculture on medium containing 1.25% NaCl (direct selection), or after gradually increasing salt concentration to 2% during serial subculture (step-wise selection). For both types of callus optimal growth occurred on 0.25% NaCl. Fresh weight to dry weight ratios (FW/DW) decreased in unselected callus grown on high (1.25–2%) salt concentrations, but not in tolerant callus. The callus obtained by direct selection was less sensitive to KCl than unselected callus. After 30–40 weeks under selection, the tolerant callus became necrotic, but healthy callus was recovered from it after transfer to salt-free medium. The callus recovered showed no retention of tolerance and preliminary experiments indicated that plants regenerated from it were more sensitive to salt irrigation than those regenerated from unselected callus.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90219-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81999934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 43
Spinach nitrate reductase: Further purification and removal of ‘nicked’ sub-units by affinity chromatography 菠菜硝酸盐还原酶:通过亲和层析进一步纯化和去除“缺口”亚基
Plant Science Letters Pub Date : 1984-11-01 DOI: 10.1016/0304-4211(84)90208-6
R.J. Fido, B.A. Notton
{"title":"Spinach nitrate reductase: Further purification and removal of ‘nicked’ sub-units by affinity chromatography","authors":"R.J. Fido,&nbsp;B.A. Notton","doi":"10.1016/0304-4211(84)90208-6","DOIUrl":"10.1016/0304-4211(84)90208-6","url":null,"abstract":"<div><p>NADH-nitrate reductase (EC 1.6.6.1) from spinach (<em>Spinacea oleracea</em> L. v. Noorman) has been purified 3400-fold by a multi-stage procedure involving streptomycin sulphate, (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>, hydroxylapatite, molecular seiving and two stages of affinity chromatography using blue-Sepharose and 5′ AMP-Sepharose. The enzyme has a specific activity of 51 μmol NO<sub>2</sub><sup>−</sup> produced min<sup>−1</sup> mg<sup>−1</sup> and a functional haem with extinction coefficients (mM) of 127 at 412 nm (oxidized) and 172 at 423 (NADH-reduced). Gel electrophoresis indicates two sub-units of approx. 110 000 and 120 000.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90208-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84854378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 23
Photodynamic damage to plant leaf tissue by rose bengal 月季对植物叶片组织的光动力损伤
Plant Science Letters Pub Date : 1984-11-01 DOI: 10.1016/0304-4211(84)90194-9
J.Paul Knox, Alan D. Dodge
{"title":"Photodynamic damage to plant leaf tissue by rose bengal","authors":"J.Paul Knox,&nbsp;Alan D. Dodge","doi":"10.1016/0304-4211(84)90194-9","DOIUrl":"10.1016/0304-4211(84)90194-9","url":null,"abstract":"<div><p>The photodynamic action of the xanthene dye rose bengal upon pea leaf tissue was investigated. Irradiation of pea leaf discs treated with aqueous solutions of the sensitizer resulted in the loss of chlorophyll. Irradiation through a filter of rose bengal solution did not result in chlorophyll loss. The loss of chlorophyll was dependent upon light intensity and oxygen levels. The enchancement of pigment loss in leaf discs supplied with deuterium oxide (D<sub>2</sub>O) supports a photodynamic mechanism involving singlet oxygen (<sup>1</sup>O<sub>2</sub>).</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90194-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75779030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 41
Isolation and partial characterization of two isolectins from Lathyrus ochrus (L.) DC. seeds 石竹(Lathyrus ochrus)中两种分离素的分离及部分鉴定直流。种子
Plant Science Letters Pub Date : 1984-11-01 DOI: 10.1016/0304-4211(84)90197-4
Pierre Rougé , Benildo Sousa-Cavada
{"title":"Isolation and partial characterization of two isolectins from Lathyrus ochrus (L.) DC. seeds","authors":"Pierre Rougé ,&nbsp;Benildo Sousa-Cavada","doi":"10.1016/0304-4211(84)90197-4","DOIUrl":"10.1016/0304-4211(84)90197-4","url":null,"abstract":"<div><p>Two isolectins have been isolated from <em>Lathyrus ochrus</em> (L.) DC. seeds by ammonium sulfate precipitation, affinity chromatography on Sephadex G-100 and subsequent chromatofocusing. The isolectins, whose isoelectric points are respectively pH 7.2 and pH 6.0, have a relative molecular mass about 49 000 and are tetramers consisting of two slightly different kinds of light (<em>M</em><sub><em>r</em></sub> 4500) and heavy (<em>M</em><sub><em>r</em></sub> 20 000) subunits. The amino acid composition, N-terminal amino acids, carbohydrate and metal content of both the isolectins and their subunits have been compared. The isolectins are non-specific in hemagglutination and agglutinate equally well human erythrocytes of different ABO groups. Their hemagglutinating activity is inhibited best by D-mannose, D-glucose and their α-methylglucosides derivatives. They share antigenic determinants in common with other <em>Lathyrus</em> lectins and other lectins belonging to the <em>Vicieae</em> tribe.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90197-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75978111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 29
Variation in content of dihydromaleimide and its glucoside in pea seedlings as affected by growth and red light 生长和红光对豌豆幼苗二氢马来酰亚胺及其糖苷含量的影响
Plant Science Letters Pub Date : 1984-11-01 DOI: 10.1016/0304-4211(84)90201-3
Kensuke Miyamoto , Koji Hasegawa , Tohru Hashimoto
{"title":"Variation in content of dihydromaleimide and its glucoside in pea seedlings as affected by growth and red light","authors":"Kensuke Miyamoto ,&nbsp;Koji Hasegawa ,&nbsp;Tohru Hashimoto","doi":"10.1016/0304-4211(84)90201-3","DOIUrl":"10.1016/0304-4211(84)90201-3","url":null,"abstract":"<div><p>Dihydromaleimide (DHMD) and its O-β-glucoside (G-DHMD) are synthesized after germination of pea (<em>Pisum sativum</em> L., cv. Progress No. 9) seed and increase in content accompanying the growth of the shoot. The free aglycone is mostly localized in the apical region, while the glucoside is distributed in the lower par of the shoot. Neither the cotyledons nor the root contain these compounds. The synthesis of DHMD is promoted by phytochrome action but that of the glucoside is not, although the glucoside accumulate more in red light than in darkness. However, the increase of DHMD is not great enough to explain the red light-induced growth inhibition.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90201-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79092209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Production of polysaccharide-degrading enzymes by Septoria Nodorum in culture and during pathogenesis 芽孢Septoria Nodorum在培养和发病过程中产生的多糖降解酶
Plant Science Letters Pub Date : 1984-11-01 DOI: 10.1016/0304-4211(84)90204-9
Paolo Magro
{"title":"Production of polysaccharide-degrading enzymes by Septoria Nodorum in culture and during pathogenesis","authors":"Paolo Magro","doi":"10.1016/0304-4211(84)90204-9","DOIUrl":"10.1016/0304-4211(84)90204-9","url":null,"abstract":"<div><p><em>Septoria nodorum</em> produced cell wall-degrading enzymes (CWDE) (oolygalacturonase (PG), xylanase (XY) and cellulase (CX)) both in mineral medium supplemented with wheat cell walls and in inoculated wheat leaves. The same in vitro and in vivo isoenzyme pattern for PG (pI 6.2 and 9.4) and XY (pI 5.2) was exhibited by isoelectric focusing resolution. CX was present in culture filtrates with one poorly resolved peak (pI 4.3–4.7). In infected tissues, an additional peak at pI 7.8 appeared. Some characteristics of <em>S. nodorum</em> polysaccharidases were compared with those of other leaf spot pathogens.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90204-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85555510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Effect of abscisic acid on nitrate uptake, respiration and photosynthesis in green algae 脱落酸对绿藻硝酸盐吸收、呼吸和光合作用的影响
Plant Science Letters Pub Date : 1984-11-01 DOI: 10.1016/0304-4211(84)90195-0
Wolfram R. Ullrich, Günter Kunz
{"title":"Effect of abscisic acid on nitrate uptake, respiration and photosynthesis in green algae","authors":"Wolfram R. Ullrich,&nbsp;Günter Kunz","doi":"10.1016/0304-4211(84)90195-0","DOIUrl":"10.1016/0304-4211(84)90195-0","url":null,"abstract":"<div><p>In the unicellular green algae, <em>Chlorella fusca</em> and <em>Ankistrodesmus (Monorhaphidium) braunii</em>, nitrate uptake in the light as in the dark was stimulated by physiological concentrations (20 μM) of abscisic acid (ABA), up to 200% by 500 μM. Dark respiration was increased to a similar extent by low ABA concentrations, but up to 13-fold by 500 μM ABA. Addition of 50 mM glucose raised the respiration level to approx. 3-fold, but did not alter the ABA stimulation. Photosynthetic O<sub>2</sub> evolution showed only insignificant inhibition by ABA at high concentrations. The intracellular level of glucose, not that of sucrose, was elevated in the presence of ABA. This suggests that increased starch degradation may be involved in the stimulation of respiration and nitrate metabolism by ABA, rather than direct interference with the plasmalemma. The data show an action of ABA in algae although to our present knowledge, it is not produced in their cells.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90195-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88167842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信