菜豆凝集素异质性与金属含量的关系

D. Manen, J.F. Manen, M.R. Morgan
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引用次数: 2

摘要

菜豆种子凝集素的研究。竞争者通常由五种四聚体分离素E4、E3L、E2L2、EL3和L4组成。然而,电泳和等电聚焦分析表明,每个纯化的原聚体具有很大的异质性。我们证明了每个四聚体隔离素存在于五种不同的电泳形式,这取决于与每个隔离素结合的Mn2+阳离子的数量。讨论了Mn2+结合对原聚物的电荷和/或分子性质的影响。在相同的实验条件下,如果我们用Ca2+离子代替Mn2+,则不会观察到上述修饰。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phaseolus vulgaris lectin heterogeneity related to their metal content

The seed lectins of Phaseolus vulgaris cv. Contender typically consist of five tetrameric isolectins E4, E3L, E2L2, EL3 and L4. However electrophoretic and isoelectric focusing analysis of each purified protomer show a great heterogeneity. We demonstrate that each tetrameric isolectin exists in five different electrophoretic forms depending on the amount of Mn2+ cation bound to each isolectin. The effects of Mn2+ binding on charge and/or molecular properties of the protomers are discussed. Under the same experimental conditions, if we use Ca2+ ions instead of Mn2+, the abovemodifications are not observed.

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