{"title":"Extracellular and Intracellular Resistances and Capacitance of Cell Membranes and Cell Wall of Tea Leaves during the Rolling Process","authors":"Y. Mizukami, Y. Sawai, Y. Yamaguchi","doi":"10.5979/CHA.2006.29","DOIUrl":"https://doi.org/10.5979/CHA.2006.29","url":null,"abstract":"揉捻工程中における茶葉の電気インピーダンスの周波数軌跡から,揉捻工程中における茶葉の細胞内外の抵抗と細胞膜・壁の静電容量を調べた。細胞外抵抗の減少は,茶葉表面からの水分蒸発の影響よりも,揉捻により細胞内液が細胞外へ押出される影響を受けた結果であると考えられた。また,揉捻工程が進むと細胞内抵抗と細胞外抵抗が等しくなることは,細胞内外において水分状態の均一化が進むものと考えられた。揉捻工程において,細胞膜・壁の静電容量の減少は組織的な構造の変化が進んだ結果であることが推察された。ただし,これらの挙動は原料生葉の影響を受けた。","PeriodicalId":201655,"journal":{"name":"Chagyo Kenkyu Hokoku (Tea Research Journal)","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130723592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Estimation of the Genome Size of Tea (Camellia sinensis), Camellia (C.japonica), and their Interspecific Hybrids by Flow Cytometry","authors":"J. Tanaka, F. Taniguchi, N. Hirai, S. Yamaguchi","doi":"10.5979/CHA.2006.1","DOIUrl":"https://doi.org/10.5979/CHA.2006.1","url":null,"abstract":"Summary Using flow cytometry and staining by propidium iodide (PI), we estimated the genome sizes of tea (Camellia sinensis (L.) Kuntze), camellia (C. japonica L.), and their interspecific hybrids by comparing relative fluorescence density per nucleus to the values of rice (Oryza sativa), soybean (Glycine max), and wheat (Triticum aestivum), whose genome sizes are known. The genome sizes of tea and camellia were nearly equal and estimated to be 4.OG bases. Garden camellia 'Higo-Ozeki' had 1.5 times the DNA content per nucleus compared to other camellia clones. Based on chromosomal observations, size of the stoma, we conclude that 'Higo-Ozeki' is triploid.","PeriodicalId":201655,"journal":{"name":"Chagyo Kenkyu Hokoku (Tea Research Journal)","volume":"8 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126234129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Estimation of Soil Nitrogen Mineralization in Andosol Tea Gardens by Kinetic Analysis","authors":"K. Uchimura, N. Miura","doi":"10.5979/CHA.2005.100_1","DOIUrl":"https://doi.org/10.5979/CHA.2005.100_1","url":null,"abstract":"黒ボク土茶園における土壌窒素の無機化特性を反応速度論的に解析し,以下の結果を得ることができた。(1)黒ボク土茶園における土壌窒素の無機化量は,うね間土壌が樹冠下土壌よりも多く,下層より表層で多かった。(2)牛ふん堆肥を施用することにより,無施用土壌に比べて,可分解性窒素量は高まるものの,無機化速度は小さくなった。(3)土壌窒素の月別無機化量は7~8月に最大となり,年間窒素無機化量の4~5割が夏季の6月から8月に無機化した。(4)黒ボク土茶園では根群域のうね間深さ0~40cm,樹冠下深さ0~10cmにおいて無機化する年間土壌窒素量は,それぞれ10~15kg/10a,約10kg/10aで,茶園全体では20~25kg/10a供給されると推定された。(5)土壌窒素の年間無機化量は土壌中の全窒素含有率と相関が高く,全窒素含有率を測定することで,概ね推定できると考えられた。","PeriodicalId":201655,"journal":{"name":"Chagyo Kenkyu Hokoku (Tea Research Journal)","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127775607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Electrical Impedance Analysis of Tea Leaves in the Steaming Process","authors":"Y. Mizukami, D. Miyama, Y. Sawai, Y. Yamaguchi","doi":"10.5979/CHA.2005.100_21","DOIUrl":"https://doi.org/10.5979/CHA.2005.100_21","url":null,"abstract":"Frequency-dependent electrical impedance spectroscopy has been used to characterize the intracellular and extracellular resistances as well as the capacitance of the cell wall and cell membranes of tea leaves in the steaming process. The intracellular resistance of the steamed leaves decreased by approximately 90% as compared to that of fresh leaves. Water in the cytoplasm was transformed into mobile water by the steaming treatment. This is the main factor that causes the decrease in the intracellular resistance. However, when the treatment duration exceeded 20 s, there was a negligible influence on the change in the resistance. Therefore, this organized behavior was considered to occur within 20 s. On the other hand, since the indissoluble pectin that constitutes the cell wall became water soluble by the steaming treatment, positive ions accumulated on the cell wall at the beginning of the treatment. Therefore, the capacitance increased temporarily due to the treatment. In addition, when the steaming treatment was prolonged, the capacitance and extracellular resistance decreased due to deterioration and shrinkage of cellular tissues. Electrochemical impedance analysis can be employed to investigate the organized behaviors that occur in tea leaves in the steaming treatment. Therefore, a numerical method to evaluate the steaming . treatment can be established using electrochemical impedance analysis.","PeriodicalId":201655,"journal":{"name":"Chagyo Kenkyu Hokoku (Tea Research Journal)","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123599748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Accuracy of SPS-GPS and Application to Tea Field Management","authors":"T. Araki, D. Miyama, M. Miyazaki","doi":"10.5979/CHA.2005.1","DOIUrl":"https://doi.org/10.5979/CHA.2005.1","url":null,"abstract":"In this study, accuracy and availability of SPS-GPS (Standard Positioning Service GPS) receiver as a positioning device was evaluated by a fixed-point and site test in a tea field. The SPS-GPS has a positioning precision of about 5.33 m (2 d RMS) with continuous observation for 12 hours at fixed-point. By using GPS, plotting and computing 1349m2(±3%)area of tea field can be done easily in 13 minutes by a single operator. Therefore, GPS has sufficient accuracy, and is a simple device to collect geographical information.","PeriodicalId":201655,"journal":{"name":"Chagyo Kenkyu Hokoku (Tea Research Journal)","volume":"2005 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128944942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Kohata, N. Hayashi, Eiji Ohtake, Shukou Kuwabara, Tomomi Ujihara, N. Teshima, Shino Kuwagata, T. Odashima
{"title":"Changes in Metal Contents during the Manufacturing Process of Sencha and in Different Positions of the Tea Shoot","authors":"K. Kohata, N. Hayashi, Eiji Ohtake, Shukou Kuwabara, Tomomi Ujihara, N. Teshima, Shino Kuwagata, T. Odashima","doi":"10.5979/CHA.2005.31","DOIUrl":"https://doi.org/10.5979/CHA.2005.31","url":null,"abstract":"Changes in metal contents during the manufacturing process of Sencha and in different positions of the tea shoot were investigated using inductively coupled plasma (ICP)-mass spectrometry (MS) and ICP-AES (atomic optical emission spectrometry) methods. The contents of Ba, Cu, Fe, Ni and Zn increased after the primary drying process, while that of Sr decreased after the final rolling process. Only Zn showed significant difference at 5% level between the contents of fresh leaves and crude tea, suggesting that the increase of Zn should be taken into consideration for the differentiation of the geographic origin of the tea. The contents of Al, Ba, Ca, Co, Mn and Sr were richer in the lower position of the tea shoot, while those of Cu, Fe, Mg, Ni, P and Zn were richer in the upper position of the tea shoot. The contents of K and Rb hardly changed regardless of the position. The stem showed a unique metal composition compared to that of the leaf. This result suggests that a tea sample processed from the stem should not be used for the differentiation of the geographic origin of the tea.","PeriodicalId":201655,"journal":{"name":"Chagyo Kenkyu Hokoku (Tea Research Journal)","volume":"225 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120956334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Miyama, T. Araki, M. Miyazaki, Kunimichi Higashi
{"title":"Development of Walking-type Fertilizer Applicator with Velocity Interlocking Device of Discharge","authors":"D. Miyama, T. Araki, M. Miyazaki, Kunimichi Higashi","doi":"10.5979/CHA.2005.11","DOIUrl":"https://doi.org/10.5979/CHA.2005.11","url":null,"abstract":"The newly developed walking-type fertilizer applicator has a fluted roll type metering device and a velocity interlocking device of fertilizer discharge. The fluted roll type metering device, adopted in developed fertilizer applicator, was equipped with rubber fluted roll with four impellers, and could meter the fertilizers of various physical properties precisely. In the range of 80rpm or less of rotational speed of fluted roll, there were proportional relationships between the rotational speeds of roll and the amount of discharge. The experimental result of field test showed that the velocity interlocking device could spread the fertilizer evenly in the field under the condition of the wide variety of work speed. The coefficients of variation were less than 4%.","PeriodicalId":201655,"journal":{"name":"Chagyo Kenkyu Hokoku (Tea Research Journal)","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114078650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}