Photodiagnosis and Photodynamic Therapy最新文献

{"title":"Recent advances in the development of multiplexed nanophotonic biosensors.","authors":"Z.K.S. Mcotshana ,&nbsp;L.N. Thwala ,&nbsp;M.H.C. Tlomatsane ,&nbsp;E. van Steen ,&nbsp;P. Mthunzi-Kufa","doi":"10.1016/j.pdpdt.2024.104246","DOIUrl":"10.1016/j.pdpdt.2024.104246","url":null,"abstract":"<div><p>The field of nanophotonics has advanced and can be utilized as a method to detect different infectious diseases. The introduction of multiplex nanophotonic diagnostics has enabled the speedy and simultaneous detection of viral infections and specific biomarkers. The quick reaction times, high sensitivity, and specificity of multiplex nanophotonic diagnostics enable real-time identification of viruses without the need for nucleic acid amplification. This review presents an overview of nanophotonic tools used to identify diseases and particular biomarkers. The paper also examines possible research areas for the development of unique, cutting-edge multiplex nanophotonic diagnostics capable of concurrently detecting various diseases or biomarkers/biomolecules. Furthermore, it discusses barriers to further advancement and offers insight into anticipated trends.</p></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1572100024002837/pdfft?md5=b9afbd24a887e8a32f55d312dbcd90c6&pid=1-s2.0-S1572100024002837-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141312627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of photodynamic therapy (PDT) of cervical low-grade squamous intraepithelial lesion (LSIL)","authors":"Weihong Qi ,&nbsp;Qiubo Lv ,&nbsp;Lan Chen ,&nbsp;Shuai Huang ,&nbsp;Huiqi Zhan ,&nbsp;Zheng Huang","doi":"10.1016/j.pdpdt.2024.104247","DOIUrl":"10.1016/j.pdpdt.2024.104247","url":null,"abstract":"<div><h3>Background</h3><p>Prevention of high-risk HPV (HR-HPV) infection and effective medical intervention of persistent HPV infection and precancerous lesions are critical for the prevention of cervical cancer.</p></div><div><h3>Aims</h3><p>The aim of this retrospective comparative study was to evaluate the outcomes of ALA PDT and observation only in the management of low-grade squamous intraepithelial lesions (LSIL).</p></div><div><h3>Methods</h3><p>In PDT Group (<em>n</em> = 138), ALA PDT was applied to patients with colposcopic biopsy confirmed cervical LSIL accompanied with HR-HPV infection longer than 1 year or HPV 16/18 subtype infection. Cervical LSIL only patients received 3 times of ALA PDT and those with concurrent cervical canal or vaginal lesions received 6 times ALA PDT. Control Group (<em>n</em> = 69) received observation only. Colposcopy, TCT and HPV typing were performed before and after treatment. Patients were followed up for up to two years.</p></div><div><h3>Result</h3><p>The observation group showed 26.1%, 34.8% and 53.6% HR-HPV negative conversion at 3-6, 12 and 24 months, respectively. LSIL regression rate of the observation group was 33.33%, 36.23% and 65.22% at 3-6, 12 and 24 months, respectively. There was 62.32%, 80.56% and 89.22% patients achieved HPV clearance at 3-6, 12 and 24 months after PDT treatment, respectively. The LSIL remission rate was 89.86%, 94.40% and 96.08% at 3-6, 12 and 24 months after ALA PDT, respectively. The abnormal TCT (≧ ASCUS) was reduced from 92% to 10.1%, 4.6% and 3.9% at 3-6, 12 and 24 months after ALA PDT, respectively. The patient age was not a factor affecting the clearance of HPV infection and the LSIL regression rate of PDT treatment.</p></div><div><h3>Conclusions</h3><p>This study demonstrates that the application of multiple ALA PDT treatments has added value in achieving both short-term and long-term HPV and lesion clearance.</p></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1572100024002849/pdfft?md5=d64c6b23190e7c67b0203fd7461e56d3&pid=1-s2.0-S1572100024002849-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141319408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A nomogram to predict anal condyloma acuminatum recurrence in HIV-negative patients following photodynamic therapy: A decade-long retrospective clinical study at a single tertiary hospital","authors":"Shu Zhou ,&nbsp;Zhiyi Xu ,&nbsp;Li Gu ,&nbsp;Xiaoyu Zhai ,&nbsp;Jing Zhao ,&nbsp;Liqun Gu ,&nbsp;Bingrong Zhou ,&nbsp;Hui Hua","doi":"10.1016/j.pdpdt.2024.104245","DOIUrl":"10.1016/j.pdpdt.2024.104245","url":null,"abstract":"<div><h3>Background</h3><p>Anal condyloma acuminatum (CA) is marked by its thorny treatment and high recurrence rate. Although 5-aminolevulinic acid photodynamic therapy (ALA-PDT) demonstrates significant efficacy and safety in treating anal CA, it does not completely prevent recurrence. This study aimed to develop and validate a nomogram model in predicting the risk of relapse in HIV-negative patients with anal CA following treatment with ALA-PDT.</p></div><div><h3>Methods</h3><p>A retrospective analysis was conducted on patients diagnosed with anal CA who received combined CO₂ laser vaporization and ALA-PDT between January 2013 and May 2023. Patients were divided into recurrence and non-recurrence groups. A nomogram was developed based on factors showing statistical significance in multivariable logistic regression analysis. The discriminative ability and clinical utility of the nomogram were assessed via ROC curves and decision curve analysis, with internal validation performed through bootstrap resampling.</p></div><div><h3>Results</h3><p>Among the 176 patients included, 33 (18.75 %) experienced recurrence, while 143 did not. Independent predictors for recurrence included HPV types, history of anal intercourse, and the number of CO₂ laser treatments received. Incorporating these predictors, the nomogram demonstrated a superior diagnostic performance (area under the curve = 0.881, 95 % CI: 0.818–0.935) and a significant net benefit in decision curve analysis.</p></div><div><h3>Conclusions</h3><p>The nomogram accurately predicts the risk of recurrence in HIV-negative patients with anal CA following ALA-PDT. It offers a valuable tool for guiding preoperative clinical decision-making and establishing personalized treatment strategies to minimize the risk of relapse.</p></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1572100024002825/pdfft?md5=d5fba20c5fb160537e80d1f415f6e9bf&pid=1-s2.0-S1572100024002825-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141319406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indocyanine green fluorescence imaging technology for the treatment of chylothorax after oesophageal cancer: A case report","authors":"Anpeng Ran,&nbsp;Lei Ma,&nbsp;Dan He,&nbsp;Waresijiang Yibulayin,&nbsp;Abulimiti Abulaiti,&nbsp;Zhenhua Wu,&nbsp;Keming Xu,&nbsp;Xiayimaierdan Yibulayin,&nbsp;Paruke Alimu,&nbsp;Xiaohong Sun","doi":"10.1016/j.pdpdt.2024.104244","DOIUrl":"10.1016/j.pdpdt.2024.104244","url":null,"abstract":"<div><p>Chylothorax is a serious postoperative complication of oesophageal cancer, and to date, there is no standardized and effective intraoperative diagnostic tool that can be used to identify the thoracic duct and determine the location of lymphatic fistulas. A 50-year-old patient with oesophageal squamous cell carcinoma developed chylothorax after thoracolaparoscopy combined with radical resection of oesophageal cancer. Twelve hours after surgery, 1200 mL of clear fluid was drained from the thoracic drainage tube, and a chyle test was sent. A thoracothoracic duct ligation procedure was performed on the first day after surgery. Although fluid accumulating in the posterior mediastinum was observed, the location of the lymphatic fistula could not be determined. During the surgery, indocyanine green (ICG) was injected into the bilateral inguinal lymph nodes, and a fluorescent lens was used to determine the location of the lymphatic fistula so the surgeon could ligate the thoracic duct. ICG fluorescence imaging technology can help surgeons effectively manage chylothorax after oesophageal cancer surgery. To our knowledge, this is the first report to describe the use of ICG fluorescence imaging technology to treat postoperative chylothorax in patients with oesophageal cancer in China.</p></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1572100024002813/pdfft?md5=79927f4b4a21e9dd81cd700569e1efa2&pid=1-s2.0-S1572100024002813-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141319409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A meta-analysis and systematic review of photodynamic therapy for diabetic foot ulcers","authors":"Chunliu Hou ,&nbsp;Lei Zhang ,&nbsp;Lifeng Wang ,&nbsp;Sai Zhao ,&nbsp;Jing Nie ,&nbsp;Mo lv ,&nbsp;Wenjie Zhang ,&nbsp;Xuesen Su ,&nbsp;Shouyuan Tian ,&nbsp;Yan Li","doi":"10.1016/j.pdpdt.2024.104228","DOIUrl":"10.1016/j.pdpdt.2024.104228","url":null,"abstract":"<div><h3>Background</h3><p>Diabetic foot ulcer (DFU) is a chronic and challenging condition, addressed through various treatments including photodynamic therapy (PDT) and standard of care (SOC), yet lacking consensus on the optimal approach. This study presents a comprehensive meta-analysis of randomized controlled trials to evaluate the efficacy and safety of PDT versus SOC in managing DFU.</p></div><div><h3>Methods</h3><p>An extensive literature search was conducted across PubMed, Embase, and the Cochrane Library databases to identify RCTs that compared the effectiveness of PDT with SOC in treating DFU. The primary metrics evaluated included changes in ulcer area, wound healing indices, and pain levels experienced by the patients.</p></div><div><h3>Results</h3><p>This meta-analysis incorporated data from 6 RCTs, encompassing 458 patients with 467 DFUs. The analysis indicated that while PDT led to a faster reduction in ulcer size compared to SOC, the difference was not statistically significant [mean difference (MD): 2.73cm², 95 % Confidence Interval (CI) -2.98 to 8.44; <em>p</em> &gt; 0.05]. However, a notable improvement was observed in the wound healing rate in the PDT group [MD: 29.26 %, 95 % CI 7.24 to 51.28; <em>p</em> = 0.01]. Based on the Visual Analog Scale (VAS), pain assessment revealed no significant difference between the two treatment groups [MD: 2.35, 95 % CI -2.36 to 7.06; <em>p</em> = 0.33].</p></div><div><h3>Conclusion</h3><p>The study suggests that PDT might offer an enhanced healing rate for DFUs compared to SOC alone, potentially leading to improved patient outcomes. Importantly, our findings highlight the superiority of photodynamic therapy in accelerating ulcer healing without an associated increase in complications.</p></div><div><h3>PROSPERO</h3><p>2023 CRD42023493930</p></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1572100024002667/pdfft?md5=8e6e781ac496afffc21ed5ce23adc5a0&pid=1-s2.0-S1572100024002667-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141312626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wide-field swept-source optical coherence tomography angiography (SS-OCTA) in the assessment of retinal vessel density and thickness in 4-to 16-year-old myopic children","authors":"Jingyu Mu ,&nbsp;Jing Wei ,&nbsp;Haoming Geng ,&nbsp;Wenhua Yi ,&nbsp;Xingzi Kang ,&nbsp;Juan Wen ,&nbsp;Junguo Duan","doi":"10.1016/j.pdpdt.2024.104240","DOIUrl":"10.1016/j.pdpdt.2024.104240","url":null,"abstract":"<div><h3>Purpose</h3><p>To analyze the characteristics of macular retinal vessel density and thickness in children with myopia.</p></div><div><h3>Methods</h3><p>A cross-sectional study was conducted. A total of 228 children aged 4–16 years who visited the Ineye Hospital of Chengdu University of Traditional Chinese Medicine from September 2022 to November 2023 were included. Those with −0.5D &lt; spherical equivalent (SE) &lt; +2.0D were included in the non-myopia group (150 eyes), those with −3.0D &lt; SE ≤ −0.5D were included in the low myopia group (246 eyes), and those with SE ≤ −3.0D were included in the moderate-to-high myopia group (60 eyes). All subjects underwent cycloplegic refraction, IOLmaster500, and Wide-field SS-OCTA (to exclude some peripheral retinal degeneration). Multiple linear regression analysis was used to analyze the correlation between macular ETDRS subfield of full retinal thickness (FRT), outer, inner retinal thickness (ORT, IRT), retinal vessel density (VD), deep and superficial retinal vessel density (DVD, SVD), and SE, axial length (AL).</p></div><div><h3>Results</h3><p>There were statistically significant differences (<em>P</em> &lt; 0.05) in FRT in the central fovea (1 mm diameter)and perifovea (Diameter 3 to 6 mm) among the non-myopia group, low myopia group, and moderate-to-high myopia group. The three groups also showed statistically significant differences (<em>P</em> &lt; 0.05) in VD in the central fovea and parafovea with a diameter of 1 to 3 mm (except the lower part). In multiple linear regression analysis adjusted for gender and age, SE and AL were found to be correlated with FRT in all ETDRS regions (except the central fovea) (<em>P</em> &lt; 0.01), and SE and AL were correlated with IRT in the central fovea and perifovea, respectively (β range −2.302 to 1.652; <em>P</em> &lt; 0.05). SE and AL were also correlated with ORT in the parafovea and perifovea, respectively (β range −4.371 to −2.344; <em>P</em> &lt; 0.05). AL was negatively correlated with VD in the central fovea and parafovea (except the inferior region) (<em>P</em> &lt; 0.05), as well as with DVD in all ETDRS regions (β range −1.314 to −1.031; <em>P</em> &lt; 0.05). AL was only negatively correlated with SVD in the parafoveal nasal region (β = −0.633, <em>P</em> &lt; 0.05). Additionally, the correlation between AL and DVD, ORT was higher than that with SVD, IRT.</p></div><div><h3>Conclusion</h3><p>The more severe the myopia, the longer the AL, the thinner the FRT in the perifovea, and the lower the VD in both the fovea and parafovea in children. In addition, DVD and ORT were more significantly correlated with AL, suggesting that they may be more closely related to the growth of AL.</p></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1572100024002771/pdfft?md5=1fa293e46575633394852dcbb3f5a5e7&pid=1-s2.0-S1572100024002771-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141312628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold atmospheric plasma in combination with laser therapy provides a window for the treatment of hyperproliferative skin disease","authors":"Liyun Wang ,&nbsp;Miaomiao Ren ,&nbsp;Cheng Chen ,&nbsp;Xingyu Yang ,&nbsp;Chenchen Zhang ,&nbsp;Jing Gao ,&nbsp;Jingwen Wang ,&nbsp;Chunjun Yang","doi":"10.1016/j.pdpdt.2024.104243","DOIUrl":"10.1016/j.pdpdt.2024.104243","url":null,"abstract":"<div><p>Modern medical understanding suggests that hyperproliferative skin diseases (HSDs) are complex syndromes characterized by localized hypertrophy or hyperplasia and infiltration of inflammatory cells. Various treatments, including systemic and topical pharmacotherapy, laser interventions, photodynamic therapy, and surgery, have been proposed for managing HSDs. However, challenges such as wound healing and recurrence after laser treatment have hindered the effectiveness of laser therapy. To overcome these challenges, we conducted a study combining laser therapy with cold atmospheric plasma (CAP) for the treatment of HSDs. Seven patients with different forms of HSDs, who had not responded well to conventional treatments, were enrolled in the study. These HSDs included cases of erythroplasia of Queyrat, pyoderma gangrenosum, keloids and hypertrophic scars, cellulitis, cutaneous lichen planus, and verruca vulgaris. Laser therapy was performed to remove the hyperplastic skin lesions, followed immediately by daily CAP treatment. The results were promising, with all patients successfully treated and no recurrence observed during the follow-up periods. The combined application of CAP and laser therapy proved to be an effective and complementary strategy for managing HSDs. This innovative approach provide evidence for addressing the limitation of laser therapy by utilizing CAP to promote wound healing and mitigate inflammatory responses.</p><p>Chinese Clinical Trial Registry (ChiCTR2300069993).</p></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1572100024002801/pdfft?md5=c9f16c67f46c7312402de747384eb528&pid=1-s2.0-S1572100024002801-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141307720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of photodynamic therapy on treatment response and survival in patients with recurrent oral squamous cell carcinoma: A systematic review","authors":"Miguel Cardoso ,&nbsp;Carlos Miguel Marto ,&nbsp;Anabela Paula ,&nbsp;Ana Sofia Coelho ,&nbsp;Inês Amaro ,&nbsp;Marta Pineiro ,&nbsp;Teresa M.V.D. Pinho e Melo ,&nbsp;Manuel Marques Ferreira ,&nbsp;Maria Filomena Botelho ,&nbsp;Eunice Carrilho ,&nbsp;Mafalda Laranjo","doi":"10.1016/j.pdpdt.2024.104242","DOIUrl":"10.1016/j.pdpdt.2024.104242","url":null,"abstract":"<div><h3>Background</h3><p>This systematic review assessed the effectiveness of photodynamic therapy (PDT) in patients with recurrent oral squamous cell carcinoma (OSCC).</p></div><div><h3>Methods</h3><p>Clinical studies on recurrent OSCC treated with PDT alone were included. Combined treatment strategies were excluded. The search was performed on Medline/Pubmed, Cochrane Library, Embase, Web of Science and ClinicalTrials.gov, manual search, and grey literature.</p></div><div><h3>Results</h3><p>The eleven included studies were observational. The risk of bias and methodological quality were evaluated using the Newcastle-Ottawa Quality Assessment Scale. The studies reported the use of hematoporphyrin derivative, Photofrin^Ⓡ, Foscan^Ⓡ and 5-aminolevulinic acid. Data on treatment response and survival was collected. Secondarily, postoperative courses and patient's quality of life/acceptance were reported whenever available. Photofrin^Ⓡ and Foscan^Ⓡ were the most used photosensitisers, with more complete responses. Lesions responding less favourably were on posterior regions or deep-seated in the tissue.</p></div><div><h3>Conclusions</h3><p>Although treatment response differs between treatment protocols, PDT stands as a viable treatment option to be considered, as it can achieve therapeutic results and disease-free, long-lasting periods. Partial treatment responses may be of interest when achieving eligibility for other treatment strategies. Despite this study's limitations, which considered four photosensitisers, Photofrin^Ⓡ was the most used but more recent photosensitisers like Foscan^Ⓡ have greater chemical stability, tissue penetration, and may be more efficacious on recurrent OSCC.</p></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1572100024002795/pdfft?md5=d0032f7eadb3eba6cc58147476c4a8c7&pid=1-s2.0-S1572100024002795-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141302343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful treatment of a lactating women with severe acne through the combination of fire needle and photodynamic therapy: A case report","authors":"Lei Tang,&nbsp;Xun Zhou,&nbsp;Ge Su","doi":"10.1016/j.pdpdt.2024.104234","DOIUrl":"10.1016/j.pdpdt.2024.104234","url":null,"abstract":"<div><p>Severe acne, characterized by cysts and nodules, can significantly impact a patient's self-image and quality of life [<span>1</span><strong>]</strong>. In China, first-line treatments for severe acne typically include oral isotretinoin, topical benzoyl peroxide, and oral or topical antibiotics [<span>2</span><strong>]</strong>. However, due to concerns about safety, oral isotretinoin and antibiotics are not recommended for lactating women, posing challenges in treating acne in this population and often leading to emotional distress. While photodynamic therapy has shown effectiveness in patients unwilling to take oral medications [<span>3</span><strong>]</strong>, treating severe acne during lactation remains a complex issue with limited research available. In this unique case, fire needle combined with photodynamic therapy was successfully utilized to address severe acne in a lactating patient. Following treatment, the patient experienced clearance of cysts, nodules, and pustules, as well as an improvement in depressive symptoms, yielding significant outcomes. Nevertheless, the efficacy and safety of this combined approach warrant further investigation through clinical trials.</p></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1572100024002710/pdfft?md5=9e20cad5d220e7efdb601661771e7223&pid=1-s2.0-S1572100024002710-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141289066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel H‑tert immortalized human sebaceous gland cell line (XL-i-20) for the investigation of photodynamic therapy","authors":"Jia Liu ,&nbsp;Detian Xu ,&nbsp;Jianna Yan ,&nbsp;Bo Wang ,&nbsp;Linglin Zhang ,&nbsp;Xiaojing Liu ,&nbsp;Haiyan Zhang ,&nbsp;Guorong Yan ,&nbsp;Jiayi Yang ,&nbsp;Qingyu Zeng ,&nbsp;Xiuli Wang","doi":"10.1016/j.pdpdt.2024.104238","DOIUrl":"10.1016/j.pdpdt.2024.104238","url":null,"abstract":"<div><h3>Background</h3><p>Acne vulgaris is a species-specific human disease. To date, there has been no established human sebocyte cell line of Asian origin. Our previous study has demonstrated the efficacy of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) in the treatment of acne vulgaris, primarily attributed to its cytotoxic properties; however, its regulatory mechanism remains largely unknown.</p></div><div><h3>Objectives</h3><p>To establish an immortalized human sebocyte cell line derived from Chinese population and investigate the underlying mechanism of ALA-PDT.</p></div><div><h3>Methods</h3><p>Human primary sebocytes were transfected with the human <em>tert</em> gene (<em>h‑tert</em>). The biological characteristics, including cell proliferation, cell markers, and sebum secretion function, were compared between primary sebocytes and the immortalized sebocytes (XL-i-20). Stimulations such as ALA-PDT, were applied respectively to both primary sebocytes and XL-i-20 cells to assess changes in their cellular functions. The transcriptome differences between primary sebocytes and XL-i-20 sebocytes were investigated using RNA-seq analysis. The XL-i-20 cell line was used to establish a sebaceous gland (SG) organoid culture, serving as a representative model of SG for the investigation of ALA-PDT.</p></div><div><h3>Results</h3><p>The <em>h‑tert</em> immortalized sebocyte cell line exhibited the ability to be consecutively cultured for more than fifty passages. Both primary and immortalized cells expressed sebocyte markers such as epithelial membrane antigens (EMA, or MUC-1), Cytokeratin 7 (CK7) and adipose differentiation-related protein associated antigens (ADRP), and maintained sebum secretion function. The proliferative capacity of XL-i-20 was found to be significantly higher than that of primary sebocytes. The responses of XL-i-20 to ALA-PDT were indistinguishable from those elicited by primary sebocytes. Cell viability and sebum secretion were decreased after ALA-PDT in both two cell lines, and lipid-related proteins (SREBP-1/PPARγ) were down-regulated. The transcriptome data consistently demonstrated upregulation of genes related to inflammatory responses and downregulation of genes involved in lipid metabolism in both cell types following PDT. The analysis of common differential genes of primary sebocytes and XL-i-20 sebocytes post ALA-PDT showed that TNF signaling pathways, MAPK signaling pathways and JAK-STAT signaling pathways were activated. The SG organoids were spherical, which expressed markers of FANS and PLET1. Ki-67 was down-regulated after ALA-PDT.</p></div><div><h3>Conclusions</h3><p>We have developed an <em>h‑tert</em> immortalized sebocyte cell line from an Asian population. The cell line, XL-i-20, maintains the essential characteristics of its parent primary sebocytes. Moreover, XL-i-20 sebocyte exhibited a significant respond to ALA-PDT, demonstrating comparable phenotypic and molecular changes to primary sebocytes. Th","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1572100024002758/pdfft?md5=b99de19e689796dd7a41b654e312fdfe&pid=1-s2.0-S1572100024002758-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141289064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}