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Oxidation of tyrosine to dopachrome by peroxidase isolated from murine melanoma. 小鼠黑色素瘤中分离的过氧化物酶氧化酪氨酸生成多巴胺。
Physiological chemistry and physics Pub Date : 1982-01-01
M R Okun, L Schley, R Ziegelstein, H Blair
{"title":"Oxidation of tyrosine to dopachrome by peroxidase isolated from murine melanoma.","authors":"M R Okun,&nbsp;L Schley,&nbsp;R Ziegelstein,&nbsp;H Blair","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Peroxidase, isolated from B16 mouse melanoma, converted tyrosine to dopachrome in the presence of either dopa or dihydroxyfumarate co-factor. A suspended homogenate of cloned, cultured B16 mouse melanoma cells also showed peroxidatic conversion of tyrosine to dopachrome in the presence of dihydroxyfumarate co-factor. The findings confirm previous histochemical, autoradiographic-histochemical, and EM-histochemical studies showing that melanoma peroxidase can convert tyrosine to melanin.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 1","pages":"8-12"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17356955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Semiconductor properties of natural melanins. 天然黑色素的半导体特性。
Physiological chemistry and physics Pub Date : 1982-01-01
T Strzelecka
{"title":"Semiconductor properties of natural melanins.","authors":"T Strzelecka","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Basic semiconductor characteristics of natural melanins isolated from bovine eye, human dark hair, and banana peel were obtained by means of the dc dark conductivity experiments and optical absorption measurements. The results were compared with results obtained for synthetic melanin. Specific conductivity in natural melanins is of the order 10(-11) omega -1 cm-1 and in synthetic melanin 10(-8) omega -1 cm-1. Thermal activation energies in the range 298-333 degrees K are eye melanin, 0.93 eV; hair melanin, 1.01 eV; banana melanin, 1.04 eV; whereas synthetic melanin has two values of activation energy: up to 311 degrees K, 0.1 eV; above 313 degrees K, 0.78 eV. Optical gaps are: in eye melanin, 1.73 eV; in hair melanin, 1.35 eV; in banana melanin, 1.55 eV; and in synthetic melanin, 1.40 eV. The observed differences between natural melanins and the synthetic one could be explained by either the presence of protein residues in natural melanins or the influence of the isolation method on their electrical properties.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 3","pages":"223-31"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18200710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of functional group on the liposome surface by colloid titration. 胶体滴定法检测脂质体表面官能团。
Physiological chemistry and physics Pub Date : 1982-01-01
Y Noda, T Katayama, Y Hirai, H Hayashi, Y Kanemasa
{"title":"Detection of functional group on the liposome surface by colloid titration.","authors":"Y Noda,&nbsp;T Katayama,&nbsp;Y Hirai,&nbsp;H Hayashi,&nbsp;Y Kanemasa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Suspensions of phospholipid liposomes (phosphatidylcholine, phosphatidylglycerol, phosphatidylethanoline and phosphatidylserine) were examined by colloid titration with polydiallyldimethylammonium chloride and potassium polyvinylsulfate as polycationic and polycationic and polyanionic titrants, respectively. In addition, the electrophoretic mobilities of the liposomes were measured with a cytopherometer. The following conclusions are drawn from the results of the titration: Phosphate and ammonium groups of phosphatidylcholine in liposomes form intramolecular or intermolecular salt linkages. Phosphate on the surface of phosphatidylglycerol liposomes shows a constant dissociation at above pH 3. Liposomes of phosphatidylethanolamine have no charge at low pH and a maximum negative charge above pH 10.5. On the surface of phosphatidylserine liposomes, the carboxyl group is completely dissociated at pH 6-7 and the phosphate group is released completely by the conversion of the ammonium ion to an amino group above pH 10.5. Stemming from these results, colloid titration can reveal the dissociation or the situation on the surface of liposomes more precisely than electrophoresis. Thus colloid titration is useful for determining the states of functional groups on the surface of phospholipid liposomes.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 4","pages":"375-9"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18202130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anesthetic-protein interaction: suppression of dye binding and limitation of applicability of the Scatchard plot. 麻醉蛋白相互作用:染料结合的抑制和Scatchard图适用性的限制。
Physiological chemistry and physics Pub Date : 1982-01-01
T Mashimo, Y Suezaki, I Ueda
{"title":"Anesthetic-protein interaction: suppression of dye binding and limitation of applicability of the Scatchard plot.","authors":"T Mashimo,&nbsp;Y Suezaki,&nbsp;I Ueda","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 6","pages":"543-51"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18202330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quantitative measurements of tissue blood flow by fast pulse heated thermistors. 用快速脉冲加热热敏电阻定量测量组织血流量。
Physiological chemistry and physics Pub Date : 1982-01-01
M Ferrari, I Giannini, F Carta, L Argiolas, A Carpi
{"title":"Quantitative measurements of tissue blood flow by fast pulse heated thermistors.","authors":"M Ferrari,&nbsp;I Giannini,&nbsp;F Carta,&nbsp;L Argiolas,&nbsp;A Carpi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Continuous measurements of local blood flow were performed by means of short pulse heated miniature thermistors. Results obtained in rat cerebral cortex or skeletal muscle show good stability of the recordings and the sensitivity of the system to phasic changes in local blood flow. Experiments on perfused rabbit kidney support the possibility of using this method for quantitative measurements.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 6","pages":"553-60"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18202331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Studies on aspartase VIII. Protease-mediated activation: comparative survey of protease specificity for activation and peptide cleavage. 天冬氨酸酶的研究。蛋白酶介导的活化:蛋白酶活化和肽裂解特异性的比较研究。
Physiological chemistry and physics Pub Date : 1982-01-01
N Yumoto, K Mizuta, M Tokushige, R Hayashi
{"title":"Studies on aspartase VIII. Protease-mediated activation: comparative survey of protease specificity for activation and peptide cleavage.","authors":"N Yumoto,&nbsp;K Mizuta,&nbsp;M Tokushige,&nbsp;R Hayashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The active species of aspartase from Escherichia coli is further 3-5 fold activated upon limited proteolysis with trypsin releasing carboxy-terminal peptides as reported previously (N. Yumoto, M. Tokushige, and R. Hayashi. Biochim. Biophys. Acta, 616, 319 (1980) ). Survey of the protease specificity for the activation revealed that subtilisin BPN' and several other proteases having far broader substrate specificity than trypsin also activated the enzyme. The results of sequence analyses revealed that subtilisin BPN' cleaved mainly the serylarginine bond near the carboxy-terminal and released an octapeptide, while trypsin cleaved mainly the arginyltyrosine bond which is just next to the subtilisin cleavage site. These results suggest that the protease-mediated activation does not necessarily require a site-specific peptidyl cleavage, but the cleavage of any bond within a certain region centered at arginine, the eighth residue from the carboxy-terminal, is sufficient.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 4","pages":"391-7"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17816957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A preliminary study of the mechanism of the hypoglycemic effect of polymeric prostaglandin PGBx in hereditary diabetic mice. Insulin response curves. 聚合前列腺素PGBx对遗传性糖尿病小鼠降血糖作用机制的初步研究。胰岛素反应曲线。
Physiological chemistry and physics Pub Date : 1982-01-01
F W Cope
{"title":"A preliminary study of the mechanism of the hypoglycemic effect of polymeric prostaglandin PGBx in hereditary diabetic mice. Insulin response curves.","authors":"F W Cope","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Polymeric prostaglandin PGBx normalizes the high blood sugar seen in hereditary diabetic mice. PGBx does not enhance the sensitivity of these mice to the hypoglycemic effect of insulin. It is therefore proposed that the hypoglycemic effect of PGBx may be an indirect consequence of induction of increased consumption by genetically defective mitochondria of two and three carbon fragments produced at the bottom of the glycolysis chain.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 5","pages":"420-2"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17817463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
L-lanthionine oxidation by snake venom L-amino acid oxidase. 蛇毒l -氨基酸氧化酶氧化l -硫氨酸。
Physiological chemistry and physics Pub Date : 1982-01-01
G Ricci, G Federici, G Lucente, M Achilli, D Cavallini
{"title":"L-lanthionine oxidation by snake venom L-amino acid oxidase.","authors":"G Ricci,&nbsp;G Federici,&nbsp;G Lucente,&nbsp;M Achilli,&nbsp;D Cavallini","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>L-Lanthionine is oxidized by snake venom L-amino acid oxidase with the release of one mole of ammonia per mole of lanthionine. Spectrophotometric, chromatographic, and analytical properties are all consistent with the identification of the mono-keto derivative of lanthionine as the first enzymatic product of the reaction. This then cyclizes into a dihydrothiazine dicarboxylic acid which is further subject to spontaneous changes. Authentic samples of the thiazine derivative have been prepared by interacting L-cysteine with bromopyruvic acid. The properties of the synthetic product are described and compared with those exhibited by the enzymatic product.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 3","pages":"193-9"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18200705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cell dehydration during tissue freezing interpreted as Bradley isotherm desorption of structured cell water. 组织冷冻过程中的细胞脱水解释为结构细胞水的布拉德利等温解吸。
Physiological chemistry and physics Pub Date : 1982-01-01
F W Cope
{"title":"Cell dehydration during tissue freezing interpreted as Bradley isotherm desorption of structured cell water.","authors":"F W Cope","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Tissue freezing without tissue death commonly shows ice formation in water outside but not inside of the cells. This observation conflicts with the expectation derived from the classical picture of the cell as a bag of liquid water with equal osmotic pressures and therefore equal depression of freezing points on both sides of the cell membrane. However, if intracellular water is structured in the form of multiple polarized layers adsorbed on cell proteins in accord with the Bradley isotherm, the prediction of theory is in harmony with experimental data.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 5","pages":"415-9"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18201979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electronically excited molecules in the formation and degradation of melanins. 电子激发的分子在黑色素的形成和降解。
Physiological chemistry and physics Pub Date : 1982-01-01
D Slawinska, J Slawinski
{"title":"Electronically excited molecules in the formation and degradation of melanins.","authors":"D Slawinska,&nbsp;J Slawinski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Light emission (chemiluminescence, CL) from model reactions in vitro, relevant to the formation and degradation of melanins has been investigated. On the basis of chemiluminescence kinetics and spectra, absorption and fluorescence changes and semi-empirical calculations, it has been shown that the common exergonic step of the chemiexcitation is the oxidative opening of the six-membered ring of indoloquinone. The data indicating cytotoxic agents formation such as H2O2 O2- and 1O2 as well as the energy transfer to strong fluorescers are presented. Physiological implications of the generation of excited molecules during the biosynthesis and degradation of melanins are discussed in terms of \"photobiochemistry without light.\"</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 4","pages":"363-74"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18202129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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