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Improved NMR contrast for mouse mammary cancer by safe physiological agents. 安全生理药物改善小鼠乳腺癌核磁共振造影。
Physiological chemistry and physics Pub Date : 1982-01-01
P T Beall
{"title":"Improved NMR contrast for mouse mammary cancer by safe physiological agents.","authors":"P T Beall","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Common safe physiological agents such as diuretics, hormones, and oral lipids can increase differences in NMR parameters between uninvolved breast tissue in tumor bearing animals and mammary carcinomas. The differential response of the normal tissue to such agents, and the relative unresponsiveness of tumors, increased NMR distinction by as much as 250%.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18202133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of SH-bearing compounds on the electron spin resonance signal of normal and cancerous tissues. 含sh化合物对正常和癌组织电子自旋共振信号的影响。
Physiological chemistry and physics Pub Date : 1982-01-01
F E Knock, P R Gascoyne
{"title":"Effects of SH-bearing compounds on the electron spin resonance signal of normal and cancerous tissues.","authors":"F E Knock,&nbsp;P R Gascoyne","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17376398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification of horse muscle acylphosphatase antibodies by affinity chromatography. 用亲和层析法纯化马肌肉酰基磷酸酶抗体。
Physiological chemistry and physics Pub Date : 1982-01-01
A Berti, G Liguri, M Stefani, P Nassi, G Ramponi
{"title":"Purification of horse muscle acylphosphatase antibodies by affinity chromatography.","authors":"A Berti, G Liguri, M Stefani, P Nassi, G Ramponi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Horse muscle acylphosphatase antibodies were obtained by immunizing rabbits with the highly purified antigen cross-linked with glutaraldehyde. Specific antibodies were purified from the immunoglobulin fraction by affinity chromatography using a matrix coupled with the pure antigen as immunoadsorbent. The purified antibodies were partially characterized by immunodiffusion and immunoprecipitin techniques. These antibodies could be used to study aspects of the muscle acylphosphatase structure, localization and other biological properties.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17370549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Temperature-dependence of ATP level, organic phosphate production and Na,K-ATPase in human lymphocytes. 人淋巴细胞ATP水平、有机磷酸盐生成和Na、k -ATP酶的温度依赖性。
Physiological chemistry and physics Pub Date : 1982-01-01
W Negendank, C Shaller
{"title":"Temperature-dependence of ATP level, organic phosphate production and Na,K-ATPase in human lymphocytes.","authors":"W Negendank,&nbsp;C Shaller","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It was previously shown that human lymphocytes maintain a normal accumulation of K+ and exclusion of Na+ between 37 degrees and 10 degrees C., and a significant net accumulation of K+ and exclusion of Na+ at even lower temperatures. The studies reported here show that the level of ATP is near-normal for at least 24 hours between 37 degrees and 10 degrees C., but that ATP synthesis and utilization are progressively and markedly decreased with decreasing temperatures below 37 degrees C. The activities of the membrane Na+- and K+-activated ATPases have typical marked temperature-dependences. Therefore, the normal accumulation of K+ and exclusion of Na+ between 37 degrees and 10 degrees C., and the normal rate of Na+ efflux at these temperatures, do not correlate with properties of the Na+,K+-ATPase or with rates of synthesis and utilization of ATP.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17376397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Binding of inactivated tyrosine aminotransferase to microsomal membranes. 失活的酪氨酸转氨酶与微粒体膜的结合。
Physiological chemistry and physics Pub Date : 1982-01-01
D Di Cola, G Federici
{"title":"Binding of inactivated tyrosine aminotransferase to microsomal membranes.","authors":"D Di Cola,&nbsp;G Federici","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Tyrosine aminotransferase from guinea pig liver spontaneously inactivates both in crude homogenates and in tissue slices. In the course of inactivation the cytosolic enzyme progressively translocates only into the microsomal fraction under an inactive form. Translocated enzyme activity can be restored by dithiothreitol addition which also produces the release of the enzyme from the microsomal particles. The specific binding of tyrosine aminotransferase to microsomal particles as a critical event for subsequent proteolytic degradation of the enzyme is postulated.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17202870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Topological aspects of ion transport in complex epithelia (frog skin). 复杂上皮(青蛙皮肤)中离子运输的拓扑方面。
Physiological chemistry and physics Pub Date : 1982-01-01
E G Huf, J R Howell
{"title":"Topological aspects of ion transport in complex epithelia (frog skin).","authors":"E G Huf,&nbsp;J R Howell","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A weak point of the current concept of the kinetics of ion flow in complex epithelial tissue membranes, such as frog skin, is the supposition that these tissue membranes and their exterior environments can be looked upon as a \"three compartment system.\" In the present study a new and more realistic conceptual framework, a \"multicompartment system,\" is applied to a computer assisted kinetic analysis of experimental data. These deal with Na+ flows in \"tight\" and \"leaky\" frog skins, prior to and after the treatment with the Na+-blocking drug amiloride. It is shown by numerical examples that unpredictable Na+ flux patterns in frog skin arise from two diverse contributing factors: 1) The constitutive physical relationships which govern the local events at the level of the plasma membranes, and 2) the much neglected topology, i.e., the \"connectedness\" of the heterogeneous compartments.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18033628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Factors influencing the water proton relaxation in nuclear fractions from tissues of normal and tumor-bearing animals. 影响正常和荷瘤动物组织核组分水质子弛豫的因素。
Physiological chemistry and physics Pub Date : 1982-01-01
S G Modak, C A Chaudhary, S R Kasturi, R S Phadke, S Shah, S S Ranade
{"title":"Factors influencing the water proton relaxation in nuclear fractions from tissues of normal and tumor-bearing animals.","authors":"S G Modak,&nbsp;C A Chaudhary,&nbsp;S R Kasturi,&nbsp;R S Phadke,&nbsp;S Shah,&nbsp;S S Ranade","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Water proton spin-lattice relaxation times (T1) were measured in nuclear fractions from liver, kidney, and spleen tissues from normal and tumor-bearing animals (TBA) at two frequencies. At 9 MHz, the T1 values for TBA nuclear fractions are less than those for normal nuclear fractions, contrary to observations at the cellular level. Trace metal ion concentration and nucleic acid content estimated for these nuclear fractions suggest that the DNA-metal ion interaction might be responsible for the observed differences in the T1 of nuclear fractions at low frequencies.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18193676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aluminum changes the conformation of calmodulin. 铝改变了钙调素的构象。
Physiological chemistry and physics Pub Date : 1982-01-01
N Siegel, C Suhayda, A Haug
{"title":"Aluminum changes the conformation of calmodulin.","authors":"N Siegel,&nbsp;C Suhayda,&nbsp;A Haug","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18199290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibition of nitroblue tetrazolium reduction by metallothionein. 金属硫蛋白对硝基蓝四氮还原的抑制作用。
Physiological chemistry and physics Pub Date : 1982-01-01
M Shiraishi, K Utsumi, S Morimoto, I Joja, S Iida, Y Takeda, K Aono
{"title":"Inhibition of nitroblue tetrazolium reduction by metallothionein.","authors":"M Shiraishi,&nbsp;K Utsumi,&nbsp;S Morimoto,&nbsp;I Joja,&nbsp;S Iida,&nbsp;Y Takeda,&nbsp;K Aono","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Metallothionein inhibited in a concentration-dependent fashion the reduction of nitroblue tetrazolium [NBT] mediated by xanthine oxidase and by NADH-phenazine methosulfate. This catalytic activity of metallothionein for dismutation of O2- is dependent on the copper content in metallothionein.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17949845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of dietary sorbitol on alkaline phosphatase and glucose-6-phosphatase in the mouse. 山梨醇对小鼠碱性磷酸酶和葡萄糖-6-磷酸酶的影响。
Physiological chemistry and physics Pub Date : 1982-01-01
A Zanobini, A M Firenzuoli
{"title":"Effect of dietary sorbitol on alkaline phosphatase and glucose-6-phosphatase in the mouse.","authors":"A Zanobini,&nbsp;A M Firenzuoli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Mice fed a sorbitol-enriched diet show measurable increases of alkaline phosphatase, glucose-6-phosphatase, and glucose-6-phosphate in the liver. The increases are positively correlated with duration of the sorbitol feeding program. The results could imply that sorbitol promotes glycogen synthesis.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17370548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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