Pharmacia最新文献_第8页

Selegiline induced differentiation of rat bone marrow mesenchymal stem cells to dopaminergic neurons in vitro Selegiline体外诱导大鼠骨髓间充质干细胞向多巴胺能神经元分化

Pharmacia Pub Date : 2023-10-04 DOI: 10.3897/pharmacia.70.e107909

Dhiya Altememy, Maryam Haji Ghasem Kashani, Pegah Khosravian

{"title":"Selegiline induced differentiation of rat bone marrow mesenchymal stem cells to dopaminergic neurons in vitro","authors":"Dhiya Altememy, Maryam Haji Ghasem Kashani, Pegah Khosravian","doi":"10.3897/pharmacia.70.e107909","DOIUrl":"https://doi.org/10.3897/pharmacia.70.e107909","url":null,"abstract":"Today, the use of mesenchymal stem cells (MSCs) for treating human diseases has attracted wide attention. The aim of this study is the expression of dopaminergic genes such as Nestin, patched Tumor Suppressor (PTCH), Sonic Hedgehog (SHH), Tyrosine Hydroxylase (TH) and Nuclear receptor-related factor 1 (NURR1) in MSCs after induction with selegiline. Rat bone marrow mesenchymal stem cells (rBMSCs) were extracted from femur and tibia bones and incubated with alpha Minimum Essential Medium (α-MEM) and 10% Fetal bovine serum (FBS). The stemness of cells at passage 4 was determined by the positive response to CD71 and CD90 markers and their differentiation into adipocytes and osteoblasts. The expression of SHH, PTCH, TH, NURR1 and Nestin genes in the cells after induction by 10-8 M selegiline for 48 hours was investigated by Reverse transcription polymerase chain reaction (RT-PCR) and Real Time-PCR methods. Isolated rBMSCs expressed CD71 and CD90 markers in culture conditions and could differentiate into adipocytes and osteoblasts. Induced cells showed neuronal morphology, positive response to Nestin and TH immunostaining. There was a significant increase of dopaminergic genes TH and NURR1 compared to the untreated cells. The results showed that selegiline with a dose of 10-8 M for 48 hours can lead to dopaminergic differentiation in rBMSCs.","PeriodicalId":20086,"journal":{"name":"Pharmacia","volume":"97 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135596830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Creatine lysinate – part II: effects on the motor coordination and muscle hypertrophy in mice 赖氨酸肌酸-第二部分:对小鼠运动协调和肌肉肥大的影响

Pharmacia Pub Date : 2023-10-04 DOI: 10.3897/pharmacia.70.e110718

Ivanka Kostadinova, Nikolai Danchev, Boycho Landzhov, Dobrina Tsvetkova, Lyubomir Marinov, Ivalina Ivanova

引用次数: 0

Isoniazid-induced liver injury risk level in different variants of N-acetyltransferase 2 (NAT2) polymorphisms: A literature review 异烟肼诱导的不同n -乙酰转移酶2 (NAT2)多态性变异的肝损伤风险水平:文献综述

Pharmacia Pub Date : 2023-10-04 DOI: 10.3897/pharmacia.70.e109869

Setiyo Budi Santoso, Prasojo Pribadi, Lalu Muhammad Irham

{"title":"Isoniazid-induced liver injury risk level in different variants of N-acetyltransferase 2 (NAT2) polymorphisms: A literature review","authors":"Setiyo Budi Santoso, Prasojo Pribadi, Lalu Muhammad Irham","doi":"10.3897/pharmacia.70.e109869","DOIUrl":"https://doi.org/10.3897/pharmacia.70.e109869","url":null,"abstract":"Individual NAT2 genotype identity data should be enriched to prevent Isoniazid-induced liver injury (IDILI) and optimize the dose of Isoniazid (INH). Therefore, this study aims to present the level of IDILI risk for specific genotype alleles. The data collection involves literature indexed by Google Scholar, Scopus, and Pubmed databases. The search uses a combination of the following keyword variants “INH” OR “INH”, “liver injury” OR “hepatotoxicity”, “polymorphism” OR “pharmacogenomic”, and “N-acetyltransferase 2” OR “NAT2”. Furthermore, the screening results of library sources were narrowed to 11 original articles that met the inclusion criteria. The IDILI risk assessment analysis due to NAT2 enzyme polymorphism following the odds ratio has a 95% confidence interval. The results showed that the IDILI risk level of the slow acetylator group was 3.11 times higher than other populations. Meanwhile, the rapid and intermediate acetylator groups were not at risk. Three variants related to *6 allele were classified as high risk; *6A/*6A risk 5.76 times, *6A/*7B (5.54 times), and *6/*7 (4 times). The three allele configurations of the *5 and *7 were also classified as a risk; *5B/*7B (5 times), *7B/*7B (3.23 times), and *5/*7 (2,74 times).","PeriodicalId":20086,"journal":{"name":"Pharmacia","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135590790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ngu-Vi-Tieu-Khat decoction, a Vietnamese traditional medicine, possesses hypoglycemic and hypolipidemic effects on streptozotocin-induced type-2 diabetic rat model 越南传统中药nku - vii - tiu - khat汤对链脲佐菌素诱导的2型糖尿病模型大鼠具有降血糖和降血脂的作用

Pharmacia Pub Date : 2023-10-03 DOI: 10.3897/pharmacia.70.e108879

Minh Hoang Le, Huyen Sanh Sam, Duy Toan Pham, Ngoc Chi Lan Nguyen, Ngoc Diem Le, Tran Nhat Phong Dao

引用次数: 0

Comparison of C-reactive protein, D-dimer, vitamin D and magnesium serum levels between migraineurs and tension-type headache sufferers 偏头痛患者和紧张性头痛患者血清中c反应蛋白、D-二聚体、维生素D和镁含量的变化Comparison

Pharmacia Pub Date : 2023-10-03 DOI: 10.3897/pharmacia.70.e103131

Khairul Putra Surbakti, Eka Mahendrayana, Chairil Amin Batubara

引用次数: 0

Colorimetric paper-based device for rapid screening of orlistat in weight loss supplements Colorimetric用于减肥补充剂中奥利司他快速筛选的纸质设备

Pharmacia Pub Date : 2023-10-03 DOI: 10.3897/pharmacia.70.e108150

Panadda Phattanawasin, Theerasak Rojanarata, Nusara Piyapolrungroj

引用次数: 0

Prevalence and risk factors of burnout among community pharmacists in Northeast region of Bulgaria - a pilot study Prevalence保加利亚东北地区社区药师职业倦怠风险因素的初步研究

Pharmacia Pub Date : 2023-10-03 DOI: 10.3897/pharmacia.70.e110145

Mariya Ivanova, Anna Todorova, Lora Georgieva

{"title":"Prevalence and risk factors of burnout among community pharmacists in Northeast region of Bulgaria - a pilot study","authors":"Mariya Ivanova, Anna Todorova, Lora Georgieva","doi":"10.3897/pharmacia.70.e110145","DOIUrl":"https://doi.org/10.3897/pharmacia.70.e110145","url":null,"abstract":"Background : Burnout is described as an emotional, mental and physical exhaustion observed in healthy people and directly related to their professional context. The objective is to study the level of burnout among community pharmacists and to identify the workplace factors affecting it. Materials and methods : Using a questionnaire, specifically designed by the authors of the study, an anonymous survey was conducted in the region of Varna, as the largest region of Northeastern Bulgaria. The questionnaire contains questions about the demographic characteristics; a standardised burnout measure tool (MBI-HSS-MP), and questions regarding the specifics of the work environment. According to MBI-HSS-MP, burnout is associated with high levels of emotional exhaustion (EE) and depersonalisation (DP) and low level of personal accomplishment (PA). Results : The participants in the pilot study were 127 masters of pharmacy. Regarding individual factors, a statistically significant relationship was found between age and the scales emotional exhaustion EE (p = 0.012), depersonalisation DP (0.028) and personal accomplishment PA (p = 0.028). Most susceptible to ЕЕ (p = 0.014) and DP (p = 0.008) were the age group 25–45 years while most satisfied with their PA were in the age group 46–55 years (p = 0.016). From the organisational factors, the large number of patients had the greatest impact on burnout (DP р = 0.000 /ЕЕ р = 0.006). The other key factors for burnout were an insufficient number of pharmacy staff, a heavy administrative burden and a wide range of medicinal products. Conclusion : It is necessary to develop training courses for young professionals to introduce them gradually into the work process and build up skills for effectively coping with stress. Proposals need to be made for reducing the administrative burden of pharmacists and maintaining staffing levels that are adequate to the patient flow and expanding the capabilities of The Specialised Electronic System for Tracing and Analysis System of Medicinal Products (SETASMP).","PeriodicalId":20086,"journal":{"name":"Pharmacia","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135697317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative analysis of total phenolic and flavonoid compounds in different extracts from ginger plant (Zingiber officinale) and evaluation of their anticancer effect against colorectal cancer cell lines Quantitative生姜植物(Zingiber officinale)不同提取物中总酚类和类黄酮类化合物的分析及其对结直肠癌细胞系的抗癌作用评价

Pharmacia Pub Date : 2023-10-02 DOI: 10.3897/pharmacia.70.e103936

Nadeen Waleed Al-Areer, Khaldun M. Al Azzam, Rima H. Al Omari, Ibrahim Al-Deeb, Lyazzat Bekbayeva, El-Sayed Negim

{"title":"Quantitative analysis of total phenolic and flavonoid compounds in different extracts from ginger plant (Zingiber officinale) and evaluation of their anticancer effect against colorectal cancer cell lines","authors":"Nadeen Waleed Al-Areer, Khaldun M. Al Azzam, Rima H. Al Omari, Ibrahim Al-Deeb, Lyazzat Bekbayeva, El-Sayed Negim","doi":"10.3897/pharmacia.70.e103936","DOIUrl":"https://doi.org/10.3897/pharmacia.70.e103936","url":null,"abstract":"Objectives : To quantify quercetin, gallic acid, rutin, naringin, and caffeic acid in the rhizome of Zingiber officinale different extracts in seven different solvents (methanol, ethanol, ethyl acetate, water, dichloromethane, chloroform, and n -hexane), for the first time, using HPLC/UV. Also, to study the anticancer activity of Zingiber officinale different extracts by evaluating its in vitro toxicity on HT-29 colorectal cancer cell line. Methods : The fresh and dried rhizomes were extracted using Soxhlet (SOX) and maceration (MAC) methods. Separation of compounds was conducted using HPLC. The cell line used for MTT cell proliferation assay antiproliferative; is HT-29 (HTB-38) colorectal adenocarcinoma. Results : The MTT test indicated that powder ginger extracted by MAC or SOX showed high cytotoxicity activity (IC 50 &lt;50) against HT-29 cells, except water using SOX, which showed mild cytotoxicity activity. The fresh ginger extracted by MAC using dichloromethane and those extracted by SOX using ethyl acetate showed strong cytotoxicity activity (IC 50 &lt;50). Conclusion : The phenolic and flavonoid contents of ginger can vary depending on the different extracts from ginger plant. Also, HPLC results revealed that quercetin was the highest in all extracts.","PeriodicalId":20086,"journal":{"name":"Pharmacia","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135828914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amiodarone prevention for atrial fibrillation relapse after surgical ablation 胺碘酮预防房颤消融术后复发

Pharmacia Pub Date : 2023-09-28 DOI: 10.3897/pharmacia.70.e110256

Ivayla Zheleva-Kyuchukova, Dimitar Kyuchukov

{"title":"Amiodarone prevention for atrial fibrillation relapse after surgical ablation","authors":"Ivayla Zheleva-Kyuchukova, Dimitar Kyuchukov","doi":"10.3897/pharmacia.70.e110256","DOIUrl":"https://doi.org/10.3897/pharmacia.70.e110256","url":null,"abstract":"Background : Atrial fibrillation (AF) is the most common heart rhythm disorder. AF ablation based on endovascular or surgical techniques is a standard of care in everyday practice. Early recurrence of atrial arrhythmias is a significant problem after ablative procedures. Prophylactic use of antiarrhythmic drugs has become a possible solution. Materials and methods : We performed a retrospective observational cohort study of 59 patients with heart disease requiring surgery under extracorporeal circulation (ECC) and have received radiofrequency ablation (RFA) for chronic or persistent AF with a successful conversion to sinus rhythm. Patients were divided into two groups: 36 were treated with amiodarone for three months postoperatively (treatment group), and 23 had no concomitant amiodarone therapy (control group). We compared the groups regarding freedom from recurrent atrial arrhythmia and the frequency of conduction disorders requiring permanent pacemaker implantation. Results : No differences between the groups were found regarding demographic characteristics, risk profile, and heart diseases indicating cardiac surgery. During follow-up, a significantly higher proportion of patients with sinus rhythm in the group receiving amiodarone was found (80.56% vs. 52.17%; p &lt; 0.041). No cases of premature discontinuation of amiodarone for side effects or noncompliance were registered in the treatment group. Conclusion : Three months of amiodarone therapy post-surgical RFA of AF is an effective and safe strategy for relapse prevention.","PeriodicalId":20086,"journal":{"name":"Pharmacia","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135425154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complementary and alternatives care for postpartum preeclampsia: A scoping review Complementary和产后子痫前期的替代护理:范围审查

Pharmacia Pub Date : 2023-09-28 DOI: 10.3897/pharmacia.70.e110572

Susilo Rini, Soetrisno Soetrisno, Tri Rejeki Andayani, Suminah Suminah

引用次数: 0