Plant disease最新文献

Diversity and Fungicide Sensitivity of Colletotrichum Species Causing Glomerella Leaf Spot and Apple Bitter Rot in Northern China. 中国北方引起肾小球叶斑病和苹果苦腐病的炭疽菌种类多样性及杀菌剂敏感性。

IF 4.4 2区农林科学

Plant disease Pub Date : 2026-05-07 DOI: 10.1094/PDIS-12-25-2469-RE

Wenjing Chen, Chenxiao Wu, Huiwen Ma, Ruokun Wu, Yuning Zheng, Zheyan Qiao, Muhammad Hussnain Qaisar, Rong Zhang, Guangyu Sun, Mingqi Zhu, Xiaofei Liang

{"title":"Diversity and Fungicide Sensitivity of Colletotrichum Species Causing Glomerella Leaf Spot and Apple Bitter Rot in Northern China.","authors":"Wenjing Chen, Chenxiao Wu, Huiwen Ma, Ruokun Wu, Yuning Zheng, Zheyan Qiao, Muhammad Hussnain Qaisar, Rong Zhang, Guangyu Sun, Mingqi Zhu, Xiaofei Liang","doi":"10.1094/PDIS-12-25-2469-RE","DOIUrl":"https://doi.org/10.1094/PDIS-12-25-2469-RE","url":null,"abstract":"Glomerella leaf spot (GLS) and apple bitter rot (ABR), collectively associated with over 20 Colletotrichum species, are two distinct diseases that cause substantial losses in apple production. In this study, phylogenetic analysis of 177 isolates collected across five provinces in northern China identified four species within the Colletotrichum gloeosporioides species complex (CGSC), namely C. aenigma, C. fructicola, C. gloeosporioides and C. siamense. C. siamense was the dominant agent of ABR (68.8%) whereas C. aenigma was the primary cause of GLS (76.9%), a pattern distinct from pathogen populations in the American continents. Fungicide profiling of the 177 isolates revealed that 41.2% of isolates showed high-level resistance to the quinone outside inhibitor (QoI) fungicide pyraclostrobin, largely attributable to the G143A mutation in the cytb gene. In contrast, sensitivity to the DMI fungicide difenoconazole remained largely unaltered. Inter- and intraspecific variation in fungicide tolerance was evident, with C. aenigma exhibiting significantly greater tolerance to both pyraclostrobin and difenoconazole compared to C. siamense. Further screening of nine additional fungicides identified prochloraz, fluazinam, penthiopyrad, and cyprodinil as highly effective against pyraclostrobin-resistant isolates. This study reveals the diversity of Colletotrichum pathogens of apple in northern China and highlights a critical need for fungicide resistance surveillance in production, and provides useful information for mitigating QoI fungicide resistance based on rational fungicide rotation.","PeriodicalId":20063,"journal":{"name":"Plant disease","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of polysaccharide peptide for the effective elimination of AYRSpV in kiwifruit. 多糖肽在猕猴桃中有效清除AYRSpV的应用。

IF 4.4 2区农林科学

Plant disease Pub Date : 2026-05-07 DOI: 10.1094/PDIS-12-25-2498-RE

Yuan Sun, Mengjia Cheng, Shengyao Tan, Xiaoling Li, Caiting Hou, Yunfeng Wu, Zhao Lei

引用次数: 0

Decadal Spatiotemporal Dynamics and Genetic Diversity of Erwinia amylovora in China (2016-2025) Revealed by CRISPR Genotyping. CRISPR基因分型揭示的中国淀粉型欧米茄（Erwinia amylovora）年代际时空动态及遗传多样性

IF 4.4 2区农林科学

Plant disease Pub Date : 2026-05-07 DOI: 10.1094/PDIS-01-26-0014-RE

Guanzhong Du, Jian Han, Benzhong Fu, Yingzhe Yuan, Xue Jiang, Qiang Sheng, Ming Luo, Baishi Hu

{"title":"Decadal Spatiotemporal Dynamics and Genetic Diversity of Erwinia amylovora in China (2016-2025) Revealed by CRISPR Genotyping.","authors":"Guanzhong Du, Jian Han, Benzhong Fu, Yingzhe Yuan, Xue Jiang, Qiang Sheng, Ming Luo, Baishi Hu","doi":"10.1094/PDIS-01-26-0014-RE","DOIUrl":"https://doi.org/10.1094/PDIS-01-26-0014-RE","url":null,"abstract":"Fire blight, caused by Erwinia amylovora, is a critical quarantine disease that emerged in China over the past decade. To characterize the population structure, genetic diversity, and virulence differentiation of E.amylovora, we performed CRISPR-based genotyping on 270 isolates collected from Xinjiang and Gansu between 2016 and 2025, China. All isolates contained three CRISPR loci (CR1, CR2, and CR4), revealing 15 distinct genotypes. Notably, 14 genotypes were identified in China for the first time, all differing from the North American reference strain Ea055 (A, a, α). The identified genotypes clustered into eight distinct lineages, with Lineage I comprising the dominant A-type (A, t, α) and three related genotypes, representing 86.7% of the total isolates. The dominant Central Asian genotype (A, t, α) represented 84.1% of isolates and remains the primary invasive lineage. However, significant regional differentiation was observed: Lineages III and V were unique to Zhangye, Gansu (2023-2025), where genotype (ie, t, α) became regionally dominant (63.3%). Furthermore, our data suggest that genotypes A and D, originating from Central Asian populations, were independently introduced into the Ili and Aksu regions of Xinjiang, China, respectively. Pathogenicity assays on detached pear leaves (Pyrus sinkiangensis) showed 61.5% of strains were highly/strongly virulent; no direct correlation was observed between CRISPR genotypes and virulence levels. These findings reveal that since its introduction, E. amylovora has undergone localized adaptation, and population differentiation has occurred at the CRISPR genotype level, resulting in a complex genetic landscape. This decadal analysis provides a critical scientific foundation for tracing fire blight spread and management strategies.","PeriodicalId":20063,"journal":{"name":"Plant disease","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and efficacy evaluation of 5% Metalaxyl·Dimethomorph Fumigant for managing Phytophthora leaf fall disease in rubber trees. 5%甲螨·啶虫啉熏蒸剂防治橡胶树疫霉病的研制及效果评价

IF 4.4 2区农林科学

Plant disease Pub Date : 2026-05-07 DOI: 10.1094/PDIS-02-26-0257-SC

Bing Xiao Chen, Lin Kun Li, Meng Wang, Congying Xu, Shanying Zhang, Xiaoyu Liang, Yu Zhang

{"title":"Development and efficacy evaluation of 5% Metalaxyl·Dimethomorph Fumigant for managing Phytophthora leaf fall disease in rubber trees.","authors":"Bing Xiao Chen, Lin Kun Li, Meng Wang, Congying Xu, Shanying Zhang, Xiaoyu Liang, Yu Zhang","doi":"10.1094/PDIS-02-26-0257-SC","DOIUrl":"https://doi.org/10.1094/PDIS-02-26-0257-SC","url":null,"abstract":"Rubber tree leaf fall disease, caused by Phytophthora botryosa, leads to severe defoliation and yield loss, yet no fungicides are currently registered for its control. Conventional spray applications often fail in tall rubber plantations due to dense canopies and hydrophobic leaf surfaces that limit coverage. This study developed a fumigant-delivered fungicide formulation designed to address these challenges. 5% metalaxyl·dimethomorph fumigant was optimized at a 4:1 active ingredient ratio. The final formulation (4% metalaxyl, 1% dimethomorph, 44% KMnO4, 4.4% starch, 28% wood powder, 9.4% NH4Cl, and the remainder kaolin) ignited readily and produced dense, upward-diffusing smoke, meeting the national quality standards for fumigant formulations. Laboratory assays showed that 0.04 g a.i. of the 5% MD FU completely inhibited the mycelial growth of P. botryosa. In detached leaf inoculation tests, 5% MD FU provided 100% protection against infection and 87% curative efficacy at 0.04 g a.i. of the 5% MD FU. A greenhouse trial on potted rubber seedlings demonstrated up to 70.54% disease control at 0.96 g a.i. of the 5% MD FU. Finally, a field trial in a mature rubber plantation confirmed effective disease suppression: the highest dosage of 1200 g/hm2 achieved 70.20% disease control, with a low dosage of 400 g/hm2 providing 60.14% control, demonstrating the formulation's effectiveness across a range of application rates. The fumigant vehicle efficiently delivered the fungicides into the canopy, resulting in uniform deposition on foliage and significant reduction of leaf disease. These findings confirm that 5% MD FU offers a superior alternative for managing Phytophthora leaf fall in rubber trees.","PeriodicalId":20063,"journal":{"name":"Plant disease","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the Effects of Potato Vine-Kill Methods on the Preharvest DNA Levels of Soil-Borne Fungal Pathogens, Incidence of Potato Virus Y in Progeny Tubers, and Yield in the San Luis Valley, Colorado. 在美国科罗拉多州圣路易斯谷地，马铃薯藤杀法对收获前土传真菌病原菌DNA水平、马铃薯Y病毒子代块茎发病率和产量影响的比较

IF 4.4 2区农林科学

Plant disease Pub Date : 2026-05-07 DOI: 10.1094/PDIS-02-26-0424-RE

Mohamad Chikh-Ali, Yuan Zeng, Jeremy Daniel, Hafiz Muhammad Usman Aslam, Amy O Charkowski

{"title":"Comparison of the Effects of Potato Vine-Kill Methods on the Preharvest DNA Levels of Soil-Borne Fungal Pathogens, Incidence of Potato Virus Y in Progeny Tubers, and Yield in the San Luis Valley, Colorado.","authors":"Mohamad Chikh-Ali, Yuan Zeng, Jeremy Daniel, Hafiz Muhammad Usman Aslam, Amy O Charkowski","doi":"10.1094/PDIS-02-26-0424-RE","DOIUrl":"https://doi.org/10.1094/PDIS-02-26-0424-RE","url":null,"abstract":"Potato (Solanum tuberosum) is susceptible to several fungal pathogens, including Alternaria solani, Alternaria alternata, Rhizoctonia solani, and Colletotrichum coccodes, which can significantly reduce yield and tuber quality. Vine-kill is commonly used by potato producers to facilitate harvest, enhance tuber maturation and skin set, and manage tuber size. This study evaluated how different vine-kill methods affect soil DNA levels of these four fungal pathogens, potato virus Y (PVY) incidence in progeny tubers, and tuber yield and size distribution in the San Luis Valley, Colorado. Treatments included pulling, flailing, and chemical desiccation with Reglone (diquat dibromide) combined with either Aim EC (carfentrazone-ethyl) or Super Tin 4L (triphenyltin hydroxide). Vine-kill methods did not significantly affect yield, size profile, or PVY incidence. However, soil pathogen DNA levels measured as DNA copy number by qPCR varied among treatments and year. In 2022, pulling resulted in the lowest levels of A. solani, A. alternata, and R. solani, and the second lowest level of C. coccodes. In 2023, pulling provided no significant benefit, but in 2025, it reduced soil levels of all four pathogens relative to desiccation and natural senescence, while flailing resulted in the lowest soil pathogen DNA levels in 2025 for both R. solani, and C. coccodes. Between the two commonly used practices, flailing generally resulted in lower fungal pathogen DNA levels detected than chemical desiccation. Overall, pulling and flailing reduced soil fungal pathogen DNA levels, suggesting that vine-kill practices might influence disease management in potato production systems.","PeriodicalId":20063,"journal":{"name":"Plant disease","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of blue light-assisted viability quantitative PCR for accurate detection of viable Erwinia amylovora. 优化蓝光辅助活力定量PCR准确检测淀粉弧菌活菌。

IF 4.4 2区农林科学

Plant disease Pub Date : 2026-05-07 DOI: 10.1094/PDIS-03-26-0455-RE

Nguyen Van Khanh, Pham Thi Hong Trang, Yong Hoon Lee

{"title":"Optimization of blue light-assisted viability quantitative PCR for accurate detection of viable Erwinia amylovora.","authors":"Nguyen Van Khanh, Pham Thi Hong Trang, Yong Hoon Lee","doi":"10.1094/PDIS-03-26-0455-RE","DOIUrl":"https://doi.org/10.1094/PDIS-03-26-0455-RE","url":null,"abstract":"Accurate quantification of viable cells is crucial for reliable disease surveillance before and during outbreaks. However, conventional quantitative PCR (qPCR) often overestimates the inoculum potential because it amplifies DNA from non-viable cells. Viability-qPCR (v-qPCR) using propidium monoazide (PMA) can address this limitation, but inconsistent dye treatment and photoactivation protocols frequently compromise the assay's reproducibility. Here, we developed a standardized blue light (BL)-assisted v-qPCR method for the selective and quantitative detection of viable Erwinia amylovora. Through systematic optimization of PMA concentration, dark incubation time, and photoactivation parameters, we found that 5-20 µM PMA, combined with a 10-min dark incubation, effectively suppressed amplification from dead cells without affecting detection of viable cells. In pure bacterial suspensions in PBS, moderate BL photoactivation (300 µmol/m²s for 20 min) effectively suppressed dead-cell DNA (99.98%), comparable to the performance of a commercial PMA-Lite™ device (>1000 µmol/m2s), while exhibiting lower phototoxicity under our tested conditions. The optimized v-qPCR showed strong linearity (R2 = 0.999) from 104 to 108 CFU/mL with an amplification efficiency of 99.0% and maintained quantitative performance in mixtures of live and dead cells. Using replicated no-template controls, the assay demonstrated a clearly defined limit of blank (LoB), and limits of detection (LoD) and quantification (LoQ) of 5×104 CFU/mL under controlled conditions in PBS, including in the presence of high dead-cell DNA. Overall, these results indicate that light intensity is a key determinant of PMA activation efficiency and potential phototoxicity and provide an experimental basis for optimizing v-qPCR assays for viable E. amylovora, with further validation required for complex plant matrices.","PeriodicalId":20063,"journal":{"name":"Plant disease","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Negative impacts of Phytophthora nicotianae race 1 on highly resistant tobacco variety KT215LC in the greenhouse. 烟草疫霉小种1对温室内高抗性烟草品种KT215LC的负面影响。

IF 4.4 2区农林科学

Plant disease Pub Date : 2026-05-07 DOI: 10.1094/PDIS-09-25-1993-RE

Kate Turner, Mitchell Richmond, Zachariah Hansen

{"title":"Negative impacts of Phytophthora nicotianae race 1 on highly resistant tobacco variety KT215LC in the greenhouse.","authors":"Kate Turner, Mitchell Richmond, Zachariah Hansen","doi":"10.1094/PDIS-09-25-1993-RE","DOIUrl":"https://doi.org/10.1094/PDIS-09-25-1993-RE","url":null,"abstract":"Phytophthora nicotianae, a soilborne oomycete, causes one of the most economically devastating diseases of tobacco known as black shank. In the past several years, burley tobacco growers have reported declining yields. Phytophthora nicotianae has two important races (race 0 and 1) with race 1 currently being the predominant race in tobacco growing areas across the U.S. Black shank is managed through a combination of crop rotation, fungicides, and selection of resistant varieties. We hypothesize that P. nicotianae race 1 could be contributing to burley tobacco yield decline in race 1-resistant varieties despite remaining mostly asymptomatic in the presence of black shank. A greenhouse trial was established to evaluate growth parameters of the resistant variety 'KT215LC' which is fully resistant to race 0 (level 10/10 resistance) and highly resistant to race 1 (level 9/10 resistance). Hybrid 404LC, which is fully susceptible to races 0 and 1, was used as a check to ensure inoculum virulence. Race 1 reduced plant height, stem diameter, leaf length, leaf number, and leaf, stem, and root mass in 'KT215LC'. These results suggest that P. nicotianae may be contributing to yield decline on varieties with high resistance to race 1. Knowledge gained from these results could help burley tobacco growers make more informed decisions when managing their tobacco crop to maximize their profits and reduce losses due to black shank.","PeriodicalId":20063,"journal":{"name":"Plant disease","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological, Molecular, and Pathogenic Characterization of Alternaria Associated with Apple Fruit Rot in China. 中国苹果果腐病稻交菌的形态、分子和致病特性研究。

IF 4.4 2区农林科学

Plant disease Pub Date : 2026-05-07 DOI: 10.1094/PDIS-02-26-0408-RE

Pengzhao Chen, Chao Hu, Liyun Guo, Xiao-Qiong Zhu

{"title":"Morphological, Molecular, and Pathogenic Characterization of Alternaria Associated with Apple Fruit Rot in China.","authors":"Pengzhao Chen, Chao Hu, Liyun Guo, Xiao-Qiong Zhu","doi":"10.1094/PDIS-02-26-0408-RE","DOIUrl":"https://doi.org/10.1094/PDIS-02-26-0408-RE","url":null,"abstract":"Alternaria fruit rot is one of the most prevalent postharvest diseases of apples in China. To clarify the species associated with this disease, we characterized Alternaria isolates from symptomatic fruit using morphological traits and multi-gene phylogenetic analyses, and further assessed their pathogenicity on apple. A total of 373 isolates were obtained from decayed apples collected in major production and storage regions, including Shandong, Shaanxi, Gansu, and Beijing. Based on colony characteristics and sporulation patterns, isolates were assigned to two groups resembling A. alternata and A. tenuissima. Phylogenetic analyses of Histone 3 (H3), Alternaria major allergen Alt a1 (Alt a1), Calmodulin (CaM), and plasma membrane ATPase (PMA) genes, however, failed to separate the two groups, and all isolates morphologically identified as A. tenuissima clustered within A. alternata. Single nucleotide polymorphism (SNP) network analysis further showed that although some isolates shared haplotypes or formed intermixed clusters, most grouped according to geographic origin or host, forming distinct Chinese, Chilean and U.S. lineages. Pathogenicity tests demonstrated that all tested isolates caused fruit rot, with virulence varying among isolates. These results indicate that A. alternata is the causal agent of postharvest Alternaria fruit rot of apples in China.","PeriodicalId":20063,"journal":{"name":"Plant disease","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disruption of two effector genes reveals their contribution to peach brown rot caused by Monilinia fructicola. 两个效应基因的破坏揭示了它们在桃褐腐病中的作用。

IF 4.4 2区农林科学

Plant disease Pub Date : 2026-05-07 DOI: 10.1094/PDIS-12-25-2432-RE

Anselmo López Sánchez-Ortiz, Rosario Torres, María Gómez, Núria Vall-Llaura, Matthias Hahn

{"title":"Disruption of two effector genes reveals their contribution to peach brown rot caused by Monilinia fructicola.","authors":"Anselmo López Sánchez-Ortiz, Rosario Torres, María Gómez, Núria Vall-Llaura, Matthias Hahn","doi":"10.1094/PDIS-12-25-2432-RE","DOIUrl":"https://doi.org/10.1094/PDIS-12-25-2432-RE","url":null,"abstract":"Brown rot, caused by Monilinia spp., is a major disease of stone fruit, yet the molecular determinants of Monilinia fructicola pathogenicity, particularly effector-related mechanisms remain poorly defined. Here, two candidate effector genes were functionally analysed by targeted mutagenesis. Resistance cassettes (hygromycin or fenhexamid) flanked by ~1 kb homology flanks were used for protoplast-mediated transformation, and repeated single-spore isolation was applied to obtain pure transformants. PCR screening and qPCR expression analyses identified two homokaryotic knockouts of mfru030g00190 and several heterokaryotic knockdown mutants of mfru004g02710, both encoding small phytotoxic effector proteins. In vitro characterization revealed no major differences between mutants and the wild type in colony morphology, radial growth, or conidiation in different media (MEA and peach-based) and temperatures (15 °C, 25 °C), indicating that these genes are not required for vegetative development. By contrast, fruit inoculation assays on peach demonstrated that deletion of mfru030g00190 significantly reduced disease incidence, whereas mfru004g02710 knockdown mutants showed a more moderate decrease and no consistent reduction in lesion size. These outcomes support the view that necrotrophic fungi deploy redundant effector repertoires, where disruption of individual genes attenuates but does not abolish pathogenicity. This study establishes a practical workflow for generating and purifying M. fructicola mutants and provides direct evidence of the contribution of effectors to infection. The results lay a foundation for broader functional genomics in Monilinia and highlight the need to identify cognate host targets in Prunus, a key step toward leveraging effector biology for resistance breeding and durable disease-management strategies.","PeriodicalId":20063,"journal":{"name":"Plant disease","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aspergillus flavus populations in matrices of the corn agroecosystem. 玉米农业生态系统基质中的黄曲霉种群。

IF 4.4 2区农林科学

Plant disease Pub Date : 2026-05-07 DOI: 10.1094/PDIS-09-25-2006-RE

Mark Weaver, Michael J Brewer, Lilly C Park, Pius A Bradicich, Krishna N Reddy

{"title":"Aspergillus flavus populations in matrices of the corn agroecosystem.","authors":"Mark Weaver, Michael J Brewer, Lilly C Park, Pius A Bradicich, Krishna N Reddy","doi":"10.1094/PDIS-09-25-2006-RE","DOIUrl":"https://doi.org/10.1094/PDIS-09-25-2006-RE","url":null,"abstract":"Infection of corn by the pathogen Aspergillus flavus can result in the contamination of the grain with aflatoxin, a potent, regulated mycotoxin. A. flavus has high genetic diversity, even within a single field, but it is unknown if the population is homogenous among the different matrices of the corn agroecosystem or if there are discernable subpopulations. We collected isolates of A. flavus from two sites for two years from soil, grain, corn silks, and Helicoverpa zea moths and larvae. This collection of over 1000 isolates of A. flavus were screened and characterized based upon aflatoxigenic potential, sclerotial morphotype and 17 simple sequence repeat (SSR) markers. The collection from the Corpus Christi, Texas study site had a higher frequency of aflatoxigenic isolates than the collection from Stoneville, Mississippi. The genotype of a commercially available biocontrol isolate of A. flavus was present at both study sites, but was 5 times more abundant at Stoneville, Mississippi than at Corpus Christi, Texas. SSR-based population genetics indicated that the A. flavus populations collected from various matrices were not differentiated and that there appeared to be significant gene flow among the populations within each site.","PeriodicalId":20063,"journal":{"name":"Plant disease","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0