PDA Journal of Pharmaceutical Science and Technology最新文献_第5页

Quantifying and Assessing Cleaning and Disinfection Residues. 清洁和消毒残留物的量化和评估。

PDA Journal of Pharmaceutical Science and Technology Pub Date : 2024-12-26 DOI: 10.5731/pdajpst.2024.99909

Madison Hoal, Aoife Duffy

引用次数: 0

Application of Fourier-Transform Infrared Spectroscopy (FT-IR) for Staphylococcus Epidermidis Typing as a Tool for Contamination Control Strategy in a Pharmaceutical Industry Facility. 傅里叶变换红外光谱（FT-IR）用于表皮葡萄球菌分型在制药工业设施污染控制策略中的应用。

PDA Journal of Pharmaceutical Science and Technology Pub Date : 2024-12-26 DOI: 10.5731/pdajpst.2024.99906

J M P Loreiro, R C C Guimarães, T B Valadao, R V S L Miranda, J M Andrade, L V Costa, M L L Brandao

{"title":"Application of Fourier-Transform Infrared Spectroscopy (FT-IR) for Staphylococcus Epidermidis Typing as a Tool for Contamination Control Strategy in a Pharmaceutical Industry Facility.","authors":"J M P Loreiro, R C C Guimarães, T B Valadao, R V S L Miranda, J M Andrade, L V Costa, M L L Brandao","doi":"10.5731/pdajpst.2024.99906","DOIUrl":"https://doi.org/10.5731/pdajpst.2024.99906","url":null,"abstract":"The typing of micro-organisms in pharmaceutical factories often relies on expensive and time-consuming molecular techniques. So, the implementation of cheap, fast and reliable typing methods in the routine would speed up the investigation procedures improving the contamination control strategy. The Fourier-transform infrared (FT-IR) spectroscopy is a method that generates spectra, that enables to micro-organisms typing within 3 h. This study aimed to evaluate the FT-IR for typing S. epidermidis strains isolated from an immunobiological pharmaceutical industry in Brazil. Fifty strains were evaluated by FT-IR using IR Biotyper®. A dendrogram was created with the raw data to cluster the separation spectrum and the cut-off value was automatically calculated. Forty-four FT-IR profiles were obtained, a ratio of 1.14 strain/profile. From the five clusters formed, Cluster 1, 2 and 3 (6 strains) were isolated from environmental monitoring of air and operators (EMO). Cluster 4 (3 strains) were isolated from EMO and bioburden assays, suggesting that the environment could be the main source of bacterial contamination in the product analyzed in bioburden assay. Cluster 5 (2 strains) were isolated from EMO and a cell culture lineage used in quality control assays, suggesting that the environment could also be the main source of cell contamination.","PeriodicalId":19986,"journal":{"name":"PDA Journal of Pharmaceutical Science and Technology","volume":"78 6","pages":"761-762"},"PeriodicalIF":0.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Contamination and Isolator Gloves: If It All Came Down to the Size of a Hole? 微生物污染和隔离手套：如果一切都取决于一个洞的大小？

PDA Journal of Pharmaceutical Science and Technology Pub Date : 2024-12-26 DOI: 10.5731/pdajpst.2023.012913

Edith Filaire, Vincent Rochette, Elodie Pierre, Franck Arethuse, Pascal Champagnat, Patrick Coppens, Eric Gohier, Cyril Mounier, Antoine Toussaint, Julien Triquet, Christian Poinsot

{"title":"Microbial Contamination and Isolator Gloves: If It All Came Down to the Size of a Hole?","authors":"Edith Filaire, Vincent Rochette, Elodie Pierre, Franck Arethuse, Pascal Champagnat, Patrick Coppens, Eric Gohier, Cyril Mounier, Antoine Toussaint, Julien Triquet, Christian Poinsot","doi":"10.5731/pdajpst.2023.012913","DOIUrl":"10.5731/pdajpst.2023.012913","url":null,"abstract":"Isolators play a critical role in protecting both the product and the environment, as well as the personnel involved in pharmaceutical manufacturing, analytical procedures, and sterility testing. Gloves attached to the windows and doors of the isolator are designed to facilitate intervention, testing, and safety. However, due to their inherent characteristics and vulnerability to puncture or loss of integrity, they are recognized as a significant potential source of contamination. In addition to the possible pathways of contamination transfer, the size of glove holes plays a critical role in determining the risk of contamination. In this study, chlorosulphonated polyethylene (CSM) gloves were exposed to an aerosol containing Bacillus subtilis or Staphylococcus aureus This assessment aimed to ascertain the integrity of the gloves' seal. It was postulated that, below a certain aperture size, gloves used in isolator systems could establish an effective seal even if the external surface of the gloves exhibited modifications. Calibrated holes of different diameters (0.3, 0.5, 1, and 1.5 mm) were created using a femtosecond laser drilling technology. The holes were located on the tip of the middle finger. Based on the context of our study, passage of microorganisms through glove holes of a certain size does occur. Under the experimental conditions chosen, the cutoff for passage was determined to be a 0.5-mm hole, regardless of the microorganism evaluated. Although this study has some limitations, including the lack of a panel of microorganisms evaluated and the investigation of a single glove type called CSM, the high level of \"worst case\" challenge conditions provides compelling data to support our results. It would now be interesting to carry out studies at different production sites to assess their risk of contamination and relate this to their glove failure.","PeriodicalId":19986,"journal":{"name":"PDA Journal of Pharmaceutical Science and Technology","volume":" ","pages":"699-706"},"PeriodicalIF":0.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Metagenomic Analysis with Oligotrophic Enrichment Approach for Detecting Specified Microorganisms. 用寡营养富集方法检测特定微生物的宏基因组分析。

PDA Journal of Pharmaceutical Science and Technology Pub Date : 2024-12-26 DOI: 10.5731/pdajpst.2024.99902

B Marasa, S Daddy Gaoh, P Alusta, Y-J Lee, J J LiPuma, D Hussong, Y Ahn

{"title":"A Metagenomic Analysis with Oligotrophic Enrichment Approach for Detecting Specified Microorganisms.","authors":"B Marasa, S Daddy Gaoh, P Alusta, Y-J Lee, J J LiPuma, D Hussong, Y Ahn","doi":"10.5731/pdajpst.2024.99902","DOIUrl":"https://doi.org/10.5731/pdajpst.2024.99902","url":null,"abstract":"In pharmaceutical manufacturing, benefit is conferred in detection of specified microorganism (i.e., Burkholderia cepacia complex (BCC), E. coli, Pseudomonas aeruginosa, Salmonella enterica) not readily identified by culture-dependent methods. It's logical to test for the presence of \"specified microorganism\" using metagenomic analysis before culturing a \"specified organism\", especially when the organism isn't easy to culture. We developed a metagenomic analysis during enrichment to identify specified organisms. The enriched bacterial community consisted predominantly of Bacillus spp. and Stenotrophomonas spp., each contributing about 97-99% to total taxon abundance in TSB and 1/10× TSB. The specified microorganisms that were observed were Clostridium spp., Burkholderia spp., and Staphylococcus spp. (0.04 - 0.07%) in TSB, otherwise Burkholderia spp., Pseudomonas spp., Salmonella spp., Staphylococcus spp. and Escherichia spp. (0.01 - 1.73%) in 1/10× TSB. PreQ0 biosynthesis (PWY-6703) and guanosine ribonucleotides de novo biosynthesis (PWY-7221) were the most abundant pathways in 1/10× TSB-24 h. BCC chiefly contributed to the toluene degradation (PWY-5180 and PWY-5182) pathways. Initial results demonstrate the potential of the metagenomic approach during enrichment in water-based environments. These results indicate that a metagenomic enrichment approach to evaluating water samples can be useful to monitor specified organisms over time, including oligotrophs such as BCC in 1/10× TSB.","PeriodicalId":19986,"journal":{"name":"PDA Journal of Pharmaceutical Science and Technology","volume":"78 6","pages":"753-754"},"PeriodicalIF":0.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of ATP-Bioluminescence Detection as a Supportive Technology for Bioburden and Sterility Testing Processes. atp生物发光检测作为生物负荷和无菌检测过程支持技术的评价。

PDA Journal of Pharmaceutical Science and Technology Pub Date : 2024-12-26 DOI: 10.5731/pdajpst.2024.99905

Madaisabel Arias, Ashley Osuna-Najarro, Scott Lute, Reyes Candau-Chaucon, Erika Pfeiler, Sarah Johnson, Talia Faison

{"title":"Evaluation of ATP-Bioluminescence Detection as a Supportive Technology for Bioburden and Sterility Testing Processes.","authors":"Madaisabel Arias, Ashley Osuna-Najarro, Scott Lute, Reyes Candau-Chaucon, Erika Pfeiler, Sarah Johnson, Talia Faison","doi":"10.5731/pdajpst.2024.99905","DOIUrl":"https://doi.org/10.5731/pdajpst.2024.99905","url":null,"abstract":"A challenge facing the biomanufacturing industry is the lengthy timeline for quality control testing that delays critical go/no-go decision making for the rapid release of drug products. The recommended USP methods for bioburden and sterility testing are surface-spread plating method and direct inoculation method, respectively. These compendial methods are reliable; however, results take approximately 5-7 days for bioburden testing (USP< 61>) and no less than 14 days for sterility testing (USP < 71>). ATP-bioluminescence detection is a rapid microbial method (RMM) that can reduce the time to result for both bioburden and sterility testing, taking 18-24 hours and 6 days, respectively. This study aims to evaluate the performance of ATP-bioluminescence in comparison to compendial methods to understand the implications of using this technology in bioburden and sterility testing processes. The research conducted consisted of simulating contamination to assess the detection capabilities of the ATP-bioluminescence assay. Results from compendia and ATP-bioluminescence detection were analyzed for comparability. Findings from this study will provide insight on the use of this platform as an alternative tool for in-process testing.","PeriodicalId":19986,"journal":{"name":"PDA Journal of Pharmaceutical Science and Technology","volume":"78 6","pages":"759-760"},"PeriodicalIF":0.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Commentary on "Understanding the Non-Equivalency of Bio-Fluorescent Particle Counts versus the Colony Forming Unit". 关于 "理解生物荧光颗粒计数与菌落形成单位的非等效性 "的评论。

PDA Journal of Pharmaceutical Science and Technology Pub Date : 2024-12-26 DOI: 10.5731/pdajpst.2024.012995

Félix A Montero Julian

引用次数: 0

Early Detection of Microbial Contaminants in Cell-Based Products Using the Milliflex^® Rapid System Combined with a Selective Lysis Solution. 使用Milliflex®快速系统结合选择性裂解溶液早期检测细胞产品中的微生物污染物。

PDA Journal of Pharmaceutical Science and Technology Pub Date : 2024-12-26 DOI: 10.5731/pdajpst.2024.99907

Cecile Delbos, Frederic Olivieri, Farah Bouhedda, Estelle Alvergnas, Renaud Chollet

{"title":"Early Detection of Microbial Contaminants in Cell-Based Products Using the Milliflex® Rapid System Combined with a Selective Lysis Solution.","authors":"Cecile Delbos, Frederic Olivieri, Farah Bouhedda, Estelle Alvergnas, Renaud Chollet","doi":"10.5731/pdajpst.2024.99907","DOIUrl":"https://doi.org/10.5731/pdajpst.2024.99907","url":null,"abstract":"The Milliflex® Rapid System is a proven automated ATP-based solution used for rapid bioburden and sterility testing. Combining membrane filtration, ATP bioluminescence and imaging, it provides microbial enumeration in significantly less time than traditional methods. Detecting microbial contamination in cell-based products remains a challenge due to filterability issues and interference from mammalian ATP. To address this, a sample processing method has been developed using a selective cell lysis solution and a free ATP removal enzyme effective at both low and high mammalian cells densities. The processing of Chinese Hamster Ovary (CHO) cells ranging from 106 to 107 cells/mL with lysis solution eliminates the mammalian ATP background in 15 minutes. This requires a ratio of 4 mL of lysis solution per mL of sample at 106 cells/mL or 9 mL of lysis solution per mL of sample at 107 cells/mL. CHO cells were inoculated separately with 6 microorganisms including Cutibacterium acnes ATCC 6919 and then processed with the lysis solution before enumeration with the Milliflex® Rapid System. Results showed a rapid enumeration of the 6 microorganisms with good recovery according to the control.","PeriodicalId":19986,"journal":{"name":"PDA Journal of Pharmaceutical Science and Technology","volume":"78 6","pages":"763-764"},"PeriodicalIF":0.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Differential Pressures Across Sterilizing Filter Membranes with Various Test Solutions. 用不同的测试溶液评估灭菌过滤膜的压差。

PDA Journal of Pharmaceutical Science and Technology Pub Date : 2024-12-26 DOI: 10.5731/pdajpst.2024.99904

Zachary Bendiks, Leesa McBurnie

{"title":"Assessment of Differential Pressures Across Sterilizing Filter Membranes with Various Test Solutions.","authors":"Zachary Bendiks, Leesa McBurnie","doi":"10.5731/pdajpst.2024.99904","DOIUrl":"https://doi.org/10.5731/pdajpst.2024.99904","url":null,"abstract":"FDA recommends monitoring differential pressure across filter membranes during sterile filtration process validation. However, few resources are available to help pharmaceutical manufacturers anticipate expected differential pressures during sterilizing filtration of different solutions. To address this gap, Meissner evaluated differential pressures across different filtration membranes using various test solutions at increasing pump speeds. Specifically, we investigated differential pressures across sterilizing-grade PVDF, PES, and PTFE membrane discs, either in series or with downstream 0.4 μm PES analysis discs commonly used in bacterial retention testing. The test solutions employed for this study include saline, grapeseed oil, FBS, and DMEM cell culture media with 10% FBS. These solutions were chosen based on their differing physicochemical properties and their relevance to the pharmaceutical industry. This work will serve as a reference for pharmaceutical manufacturers and help them anticipate differential pressures across sterilizing filter membranes at different pump speeds based on the physicochemical properties of their drug products.","PeriodicalId":19986,"journal":{"name":"PDA Journal of Pharmaceutical Science and Technology","volume":"78 6","pages":"757-758"},"PeriodicalIF":0.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review of Artificial Intelligence and Machine Learning in Product Life Cycle Management. 人工智能和机器学习在产品生命周期管理中的应用综述。

PDA Journal of Pharmaceutical Science and Technology Pub Date : 2024-10-22 DOI: 10.5731/pdajpst.2023.012922

Maria Ana Martins da Cruz Borges Batalha, Daniel Alexandre Marques Pais, Rui Alexandre Estrela de Almeida, Ângela Sofia Gomes Martinho

{"title":"A Review of Artificial Intelligence and Machine Learning in Product Life Cycle Management.","authors":"Maria Ana Martins da Cruz Borges Batalha, Daniel Alexandre Marques Pais, Rui Alexandre Estrela de Almeida, Ângela Sofia Gomes Martinho","doi":"10.5731/pdajpst.2023.012922","DOIUrl":"10.5731/pdajpst.2023.012922","url":null,"abstract":"The pursuit of harnessing data for knowledge creation has been an enduring quest, with the advent of machine learning (ML) and artificial intelligence (AI) marking significant milestones in this journey. ML, a subset of AI, emerged as the practice of employing mathematical models to enable computers to learn and improve autonomously based on their experiences. In the pharmaceutical and biopharmaceutical sectors, a significant portion of manufacturing data remains untapped or insufficient for practical use. Recognizing the potential advantages of leveraging the available data for process design and optimization, manufacturers face the daunting challenge of data utilization. Diverse proprietary data formats and parallel data generation systems compound the complexity. The transition to Pharma 4.0 necessitates a paradigm shift in data capture, storage, and accessibility for manufacturing and process operations. This paper highlights the pivotal role of AI in converting process data into actionable knowledge to support critical functions throughout the whole product life cycle. Furthermore, it underscores the importance of maintaining compliance with data integrity guidelines, as mandated by regulatory bodies globally. Embracing AI-driven transformations is a crucial step toward shaping the future of the pharmaceutical industry, ensuring its competitiveness and resilience in an evolving landscape.","PeriodicalId":19986,"journal":{"name":"PDA Journal of Pharmaceutical Science and Technology","volume":" ","pages":"604-612"},"PeriodicalIF":0.0,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142009211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Root Cause Analysis of the Visible Particles Commonly Encountered in the Biopharmaceutical Industry. 生物制药行业常见可见微粒的识别和根本原因分析。

PDA Journal of Pharmaceutical Science and Technology Pub Date : 2024-10-22 DOI: 10.5731/pdajpst.2023.012894

Bo Wang, Shanshan Zhang, Mengyi Chen, Ming Lei, Tian Gao, Wei Fan, Jincui Huang, Xiaolin Cao

{"title":"Identification and Root Cause Analysis of the Visible Particles Commonly Encountered in the Biopharmaceutical Industry.","authors":"Bo Wang, Shanshan Zhang, Mengyi Chen, Ming Lei, Tian Gao, Wei Fan, Jincui Huang, Xiaolin Cao","doi":"10.5731/pdajpst.2023.012894","DOIUrl":"10.5731/pdajpst.2023.012894","url":null,"abstract":"Visible particle is an important issue in the biopharmaceutical industry, and it may occur across all the stages in the life cycle of biologics. Upon the occurrence of visible particles, it is often necessary to conduct chemical identification and root cause analysis to safeguard the safety and efficacy of the biotherapeutic products. In this article, we present a number of typical particles and relevant root cause analysis in the categories of extrinsic, intrinsic, and inherent particles that are commonly encountered in the biopharma industry. In particular, the optical images of particles obtained both in situ and after isolation are provided, along with spectral and elemental information. The particle identification was carried out with multiple microscopic and microspectroscopic techniques, including stereo optical microscopy, Fourier-transform infrared microscopy, confocal Raman microscopy, scanning electron microscopy, and energy dispersive X-ray spectroscopy. Both commercial and in-house spectral databases were used for comparison and identification. In addition to particle identification, we placed significant efforts on the root cause analysis of the addressed particles with the intention to provide a relatively whole picture of the particle-related issues and practical references to particle mitigation for our peers in the biopharmaceutical industry.","PeriodicalId":19986,"journal":{"name":"PDA Journal of Pharmaceutical Science and Technology","volume":" ","pages":"586-603"},"PeriodicalIF":0.0,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141186449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0