Pancreatology最新文献

{"title":"Methionine restriction inhibits pancreatic cancer proliferation while suppressing JAK2/STAT3 pathway","authors":"Manabu Maebashi ,&nbsp;Kentaro Miyake ,&nbsp;Jun Yamamoto ,&nbsp;Kota Sahara ,&nbsp;Tomoko Akiyama ,&nbsp;Yayoi Kimura ,&nbsp;Itaru Endo","doi":"10.1016/j.pan.2024.11.023","DOIUrl":"10.1016/j.pan.2024.11.023","url":null,"abstract":"<div><h3>Background</h3><div>Methionine restriction (MR) has been demonstrated to exhibit anti-tumor effects in various types of cancer, including pancreatic cancer (PC). However, the detailed mechanism induced by MR remains still unclear. This study aims to reveal the underlying mechanism of MR on PC by proteomic analysis.</div></div><div><h3>Material &amp; methods</h3><div>Human PC cell lines were cultured in both standard and MR media to evaluate the effect of MR. The differences in protein expression were evaluated through proteomic analysis. Ingenuity Pathway Analysis (IPA) was performed to identify proteins potentially associated with tumor growth in vitro. The proteins associated with the anti-tumor effect were validated using western blotting, real-time PCR, and ELISA. An experimental model involving subcutaneous PC mice was established for the assessment of the effectiveness of the MR diet and the expression of target proteins through immunohistochemical staining.</div></div><div><h3>Results</h3><div>Cell proliferation was suppressed in the MR media compared to the standard media. IPA analysis showed that STAT3 was decreased in the Apoptotic Pathway of Pancreatic Cancer Cell lines in the MR group. Western blotting showed MR decreased STAT3 expression. Real-time PCR showed that MR decreased JAK2 and STAT3 mRNA expression in Panc-1 and Mia-PaCa 2, but not in Capan-1. ELISA revealed that NF-kB expression was decreased in the MR group. In the in vivo study, the final estimated tumor volume in the MR group was significantly lower than the control group (p &lt; 0.01). Immunostaining of resected specimens showed that STAT3 expression was suppressed in the MR group.</div></div><div><h3>Conclusion</h3><div>MR suppressed the JAK2/STAT3 pathway and decreased NF-kB in some PC cell lines.</div></div>","PeriodicalId":19976,"journal":{"name":"Pancreatology","volume":"25 1","pages":"Pages 108-117"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concomitant virus infection increases mortality and worsens outcome of acute pancreatitis: A systematic review and meta-analysis","authors":"Gefu Cai ,&nbsp;Eszter Ágnes Szalai ,&nbsp;Petrana Martinekova ,&nbsp;Ximeng Li ,&nbsp;Xinyi Qian ,&nbsp;Dániel Sándor Veres ,&nbsp;Zoltán Péterfi ,&nbsp;Jaishree Biswakarma ,&nbsp;Rita Nagy ,&nbsp;Alexandra Mikó ,&nbsp;Szabolcs Ábrahám ,&nbsp;Bálint Erőss ,&nbsp;Péter Hegyi ,&nbsp;Andrea Szentesi","doi":"10.1016/j.pan.2024.12.005","DOIUrl":"10.1016/j.pan.2024.12.005","url":null,"abstract":"<div><h3>Background</h3><div>Acute pancreatitis (AP) is a major health threat, with a high mortality rate in severe forms. Though alcohol and bile-induced factors are the most common causes, increasing evidence suggests that viral infections such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and human immunodeficiency virus (HIV) may also trigger AP development. Our study aims to explore this association in greater detail.</div></div><div><h3>Methods</h3><div>After the PROSPERO registration, we systematically searched PubMed, Embase, Cochrane Library, China Science and Technology Journal Database, China National Knowledge Infrastructure, and Wanfang Data Knowledge Service Platform in February 2023. We included studies with the following PECO framework: Population: AP patients, Exposure/Comparison: with/without virus infection, Outcome: mortality, severity, and complications of AP. Pooled odds ratios (OR) were calculated with 95 % confidence intervals (CIs).</div></div><div><h3>Results</h3><div>Altogether, 29 cohorts with 2,295,172 patients were identified for the meta-analysis and 858 cases for the qualitative synthesis. Patients with concurrent SARS-CoV-2 infection and AP exhibited heightened odds of in-hospital mortality (OR: 3.15, CI: 2.08–4.76), and necrosis (OR: 1.83, CI: 1.13–2.97). Mild AP was less prevalent in the SARS-CoV-2 group (OR: 0.37, CI: 0.14–0.97) compared to moderately severe and severe AP together. Contrarily, no evidence was found that concomitant HIV infection elevated in-hospital mortality (OR: 1.12, CI: 0.92–1.37) or sepsis occurrence (OR:1.21, CI: 0.41–3.59).</div></div><div><h3>Conclusion</h3><div>Patients co-diagnosed with AP and SARS-CoV-2 infection require heightened attention due to an increased risk of mortality and complications. No evidence was found that HIV infection elevated the risk of a more severe outcome.</div></div>","PeriodicalId":19976,"journal":{"name":"Pancreatology","volume":"25 1","pages":"Pages 20-28"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance of administering postoperative pancreatic enzyme replacement therapy for fat digestion and absorption functions in patients who underwent initial total pancreatectomy","authors":"Ryuta Shintakuya ,&nbsp;Kenichiro Uemura ,&nbsp;Tatsuaki Sumiyoshi ,&nbsp;Kenjiro Okada ,&nbsp;Kenta Baba ,&nbsp;Takumi Harada ,&nbsp;Yasutaka Ishii ,&nbsp;Shiro Oka ,&nbsp;Koji Arihiro ,&nbsp;Yoshiaki Murakami ,&nbsp;Shinya Takahashi","doi":"10.1016/j.pan.2024.12.011","DOIUrl":"10.1016/j.pan.2024.12.011","url":null,"abstract":"<div><h3>Objectives</h3><div>To evaluate the effects of postoperative pancreatic enzyme replacement therapy on fat digestion and absorption in patients following initial total pancreatectomy.</div></div><div><h3>Methods</h3><div>Data were retrospectively collected from patients who underwent initial total pancreatectomy at our department between 2012 and 2020. Fat digestion, absorption functions, serum nutritional markers, HbA1c levels, and hepatic steatosis before and after the initial total pancreatectomy were evaluated. The rate of change in these parameters pre- and 1-year postoperatively were compared between patients with initial total pancreatectomy and pancreaticoduodenectomy. Patients underwent the ¹³C-labeled mixed triglyceride breath test to evaluate fat digestion and absorption functions. Hepatic steatosis was assessed using computed tomography.</div></div><div><h3>Results</h3><div>Of 17 consecutive patients who underwent initial total pancreatectomy, 12 were men, and the median age was 70 years. All 17 patients received 1800 mg pancrelipase when food intake was resumed after surgery. The pre- and 1-year postoperative median % dose ¹³C cum 7 h (%), serum nutritional markers, HbA1c levels, and liver computed tomography findings did not differ significantly. Two patients had nonalcoholic fatty liver disease after surgery, without serious disease progression. In total, 48 patients who underwent pancreaticoduodenectomy were found eligible. The median change in % dose ¹³C cum 7 h (%), serum nutritional markers, HbA1c levels, and liver computed tomography findings pre and 1-year postoperatively showed no significant differences between the initial total pancreatectomy and pancreaticoduodenectomy groups.</div></div><div><h3>Conclusions</h3><div>High-dose pancreatic enzyme replacement therapy after initial total pancreatectomy might maintain fat digestion and absorption functions and nutritional status and prevent hepatic steatosis.</div></div>","PeriodicalId":19976,"journal":{"name":"Pancreatology","volume":"25 1","pages":"Pages 160-166"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond 4 weeks? Rethinking the timing of LAMS removal in WOPN.","authors":"Saswati Kar, Subhabrata Biswal, Hemanta Kumar Nayak","doi":"10.1016/j.pan.2025.01.010","DOIUrl":"https://doi.org/10.1016/j.pan.2025.01.010","url":null,"abstract":"","PeriodicalId":19976,"journal":{"name":"Pancreatology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter re: Outcomes of percutaneous endoscopic versus endoscopic transmural necrosectomy for necrotizing pancreatitis.","authors":"Saxon Connor, John Neoptolemos","doi":"10.1016/j.pan.2025.01.001","DOIUrl":"https://doi.org/10.1016/j.pan.2025.01.001","url":null,"abstract":"","PeriodicalId":19976,"journal":{"name":"Pancreatology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patient-derived organoids from pancreatic cancer after pancreatectomy: Feasibility and organoid take rate in treatment-naïve periampullary tumors.","authors":"Marcus T T Roalsø, Marina Alexeeva, Celine Oanæs, Martin Watson, Dordi Lea, Claudia Zaharia, Hanne R Hagland, Kjetil Søreide","doi":"10.1016/j.pan.2024.12.018","DOIUrl":"https://doi.org/10.1016/j.pan.2024.12.018","url":null,"abstract":"<p><strong>Background/objective: </strong>Patient-derived organoids (PDOs) have emerged as essential for ex vivo modelling for pancreatic cancer (PDAC) but reports on efficacy and organoid take rate are scarce. This study aimed to assess the feasibility of establishing PDOs from resected specimens in periampullary tumors.</p><p><strong>Methods: </strong>Patients undergoing surgery for suspected periampullary cancer were included. PDO protocol amendments were tested, with organoid take rate as outcome measure. Samples from resected specimens were processed and expanded per protocol. Pooled estimate of take rates of PDOs in PDAC was derived from literature search.</p><p><strong>Results: </strong>23 specimens were available for PDO, of which 10 were PDAC. In 15 patients other histopathology was found: neuroendocrine tumors (NET; n = 2), neuroendocrine carcinoma (NEC; n = 1), intraductal papillary mucinous neoplasm (IPMN; n = 4), distal cholangiocarcinoma (dCCA; n = 1), ampullary carcinoma (n = 1), duodenal carcinoma (n = 1), intra-ampullary papillary tubular neoplasm (IAPN; n = 1), indeterminate PDAC/ampullary carcinoma(n = 1), and one patient with chronic inflammation/fibrosis. Organoid cultures were grown from 7 of 10 (70 %) PDAC, 1 dCCA, 1 NEC, 1 duodenal carcinoma, 1 indeterminate tumor type and 1 ampullary carcinoma (i.e. 12/18; 66.7 % across periampullary cancers). Overall take rate of PDOs was 12 of 23 (52.2 %) for all tumors. A pooled mean estimate PDO take rate of 62.3 % (95 % CI:54.8-69.3 %) was reported across available studies in the literature.</p><p><strong>Conclusion: </strong>In the current study, we found that PDOs could be established from resected pancreatic tumors in over half of resected periampullary tumors, and highest in PDACs. As such, generating a pancreatic cancer PDO biobank for translational research was feasible after cryopreservation.</p>","PeriodicalId":19976,"journal":{"name":"Pancreatology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endoscopic ultrasound is useful for the risk stratification of mucinous pancreatic cystic lesions: A long-term prospective study.","authors":"Sahar Wesali, Antonio Molinaro, Björn Lindkvist, Per Hedenström, Riadh Sadik","doi":"10.1016/j.pan.2024.12.006","DOIUrl":"https://doi.org/10.1016/j.pan.2024.12.006","url":null,"abstract":"<p><strong>Objectives: </strong>The aims of this prospective observational study were to test worrisome features on endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) as a stratification tool in patients with mucinous pancreatic cystic lesions (PCLs), and to assess these patients' long-term risk of adenocarcinoma and mortality.</p><p><strong>Methods: </strong>Patients with suspected PCLs on cross-sectional imaging who underwent EUS-FNA at Sahlgrenska University Hospital between February 2007 and February 2018 were consecutively enrolled. The main inclusion criterion was the final diagnosis of a mucinous PCL. The results from EUS-FNA were analyzed in the context of outcome gathered from medical records of follow-up until February 2021.</p><p><strong>Results: </strong>Of 334 patients undergoing EUS-FNA, 171 (51 %) had a final diagnosis of a mucinous PCL. 29/171 (17 %) patients were diagnosed with HGD or adenocarcinoma <6 months after EUS-FNA, with 28/29 (97 %) patients having at least one worrisome feature on EUS-FNA. A solid component in mucinous PCLs on EUS was independently associated with the presence of HGD or adenocarcinoma (OR 23.6, 95 % CI 6.1-91.6, p < .001). A total of 4/142 (3 %) patients developed adenocarcinoma during the follow-up period (median = 61.4 months). Overall, in 80/82 (98 %) of the patients without worrisome features on EUS-FNA, HGD or adenocarcinoma was not detected. Six of the 21 (29 %) patients with HGD or adenocarcinoma who underwent surgery as initial management died from pancreatic cancer during follow-up.</p><p><strong>Conclusions: </strong>EUS-FNA is useful for the risk stratification of mucinous PCLs. The low incidence of adenocarcinoma over time after a negative EUS-FNA may allow for a less resource intensive surveillance strategy.</p>","PeriodicalId":19976,"journal":{"name":"Pancreatology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends and impact of endoscopic ultrasound utilization for suspected intraductal papillary mucinous neoplasms.","authors":"Lillian Wang, Priyanka Vatsavayi, Shounak Majumder, Ferga C Gleeson, Elizabeth Rajan, Barham K Abu Dayyeh, Andrew C Storm, Shifa Umar, Saran T Velaga, William S Harmsen, Santhi S Vege, Vinay Chandrasekhara","doi":"10.1016/j.pan.2024.12.001","DOIUrl":"https://doi.org/10.1016/j.pan.2024.12.001","url":null,"abstract":"<p><strong>Background: </strong>Multiple management guidelines for intraductal papillary mucinous neoplasms (IPMNs) have been published to improve risk stratification and resource utilization. This study aims to evaluate trends in endoscopic ultrasound (EUS) use and agreement between cross-sectional imaging and EUS for specific pancreas cystic lesion (PCL) features.</p><p><strong>Methods: </strong>This retrospective cohort study included consecutive adults undergoing EUS for suspected IPMN detected with cross-sectional imaging (CT/MRCP) between 2013 and 2015 (Cohort 1) and 2018-2020 (Cohort 2). Clinical, radiographic, EUS, cytologic, and outcomes data were collected. IPMN were defined as high-risk/worrisome (having any Fukuoka high risk (HR) or worrisome feature (WF)), low risk (cyst size 10-29.9 mm, without Fukuoka HR or WF), or very low risk (cyst size <10 mm, without Fukuoka HR or WF). Chi-square, Fisher's exact test, Wilcoxon rank-sum test and Kappa statistics were used. Impact of the COVID-19 pandemic was assessed via post-hoc sensitivity analysis.</p><p><strong>Results: </strong>Of 711 patients, 292 (41.1 %) were in Cohort 1. More patients in Cohort 1 underwent EUS for non-high risk/non-worrisome PCL on pre-EUS imaging (65.8 % vs. 54.9 %, P < 0.01). Good agreement between pre-EUS imaging and EUS for WF (84.1 %) and HR (96.6 %) was seen. EUS-FNA impacted surveillance and surgical referral in 29.5 % (P = 0.51). Median surveillance duration was 917 days (IQR 367, 1439 days), during which pancreatic ductal adenocarcinoma (PDAC) was diagnosed in 7.5 % (P = 0.11).</p><p><strong>Conclusions: </strong>In this series EUS over-utilization for low risk IPMN improved over time reflecting more conservative guidelines, without changing PDAC diagnosis rates. There was good agreement between cross-sectional imaging and EUS for specific PCL features.</p>","PeriodicalId":19976,"journal":{"name":"Pancreatology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The modern human-specific cognitive genes and pancreatic cancer risk","authors":"Pelin Ünal ,&nbsp;Federico Canzian","doi":"10.1016/j.pan.2024.05.028","DOIUrl":"10.1016/j.pan.2024.05.028","url":null,"abstract":"","PeriodicalId":19976,"journal":{"name":"Pancreatology","volume":"24 ","pages":"Pages e12-e13"},"PeriodicalIF":2.8,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasmacytoid dendritic cells reduce acinar cell regeneration via IL33 and IFNA in an autoimmune pancreatitis model","authors":"Elisabeth Orgler ,&nbsp;Nicole Schreiner ,&nbsp;Hellen Ishikawa-Ankerhold ,&nbsp;Lisa Fahr ,&nbsp;Georg Beyer ,&nbsp;Julia Mayerle ,&nbsp;Ivonne Regel","doi":"10.1016/j.pan.2024.05.030","DOIUrl":"10.1016/j.pan.2024.05.030","url":null,"abstract":"","PeriodicalId":19976,"journal":{"name":"Pancreatology","volume":"24 ","pages":"Pages e13-e14"},"PeriodicalIF":2.8,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}