{"title":"Comparative Analysis of Otolith Micro-Characteristic in Schizothorax grahami and Spinibarbus sinensis","authors":"Qun Lu","doi":"10.17582/journal.pjz/20220411130406","DOIUrl":"https://doi.org/10.17582/journal.pjz/20220411130406","url":null,"abstract":"","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67558225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Ovsynch Estrus Synchronization in Holstein Friesian and Holstein Friesian-Jersey Crossbred Cows at District Thatta, Sindh, Pakistan","authors":"S. Shah","doi":"10.17582/journal.pjz/20220523070520","DOIUrl":"https://doi.org/10.17582/journal.pjz/20220523070520","url":null,"abstract":"The current study was conducted to assess the ovsynch estrus synchronization in imported pure Holstein Friesian and Holstein Friesian-Jersey crossbred cows at district Thatta Sindh. A total 30 Holstein Friesian and Holstein Friesian-Jersey crossbred cattle were selected and divided into three groups i.e., A (10 pure Holstein Friesian cows), B (10 Holstein Friesian-Jersey crossbred cows), and C (5 pure Holstein Friesian and 5 Holstein Friesian–Jersey crossbred cows) as control group. All selected animals were scanned through ultrasound to ensure the normality of reproductive tract. Animals of group A and group B were treated with Ovsynch protocol {GnRH; (Dalmarelin, FATRO) intramuscular at day 0 followed by PGF2α (Dalmazin; FATRO) intramuscular at day 7 and 2 nd GnRH intramuscular at day 9} for estrus synchronization while animals of group C was kept as untreated control (intramuscular injection of normal saline as placebo on day 0, 7 and 9). Animals of all group underwent fixed time insemination at 16 h of last treatment. Results showed that estrus response vary significantly among the groups (P<0.05). Overall estrus response was observed significantly higher in group B (80%), followed by A (60%) and C (10%). Intensity of estrus was determined by observing the estrus signs. Results showed significantly higher intensity of estrus signs in Group B than A and C for mucus discharge 90%, 65%,10%, flehmen 90%, 60%,10%, standing heat80%, 60%, 10% and sniffing vagina 80%, 70%, 10%, respectively. Estrus duration differs significantly among the treatment groups (P<0.05). Significantly maximum estrus duration was noted in group B (15.02 ± 0.21 h), then A (13.58 ± 2.22 h) and C (10 ± 0.00 h). In all groups blood samples were collected from the milk vein on day 0 (the first treatment), day 7, day 11 (one day after artificial insemination), day 18 and day 30 from all groups for determining progesterone level. Statistical analysis showed non-significant differences in progesterone level among groups at day 0. Furthermore, on Day 7, Group C differed significantly in progesterone level than A and B; however non-significant difference were observed in group A and B, while in group C difference was significant. On day 11 significantly highest values of blood progesterone were seen in group C (2.63 ± 0.22) followed by A (1.65 ± 0.35) and then B (1.48 ± 0.22). Statistical analysis showed that group C was significantly different from A and B, but there was non-significant difference between A and B. At day 18 highest P4 values were seen in B (4.20 ± 0.44) followed by A (3.33 ± 0.39) and then C (1.88 ± 0.59). Statistical analysis showed that group B and A had non-significant difference among each other, while both groups had significant differences with group C. Blood progesterone level for all the three groups on day 30 showed highest values in group B (10.90 ± 2.44) followed by A (8.28 ± 3.33) and C (4.53 ± 0.44). Statistical analysis showed significant difference am","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67560362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Summer Diet of South China Sika Deer (Cervus nippon kopschi) in Taohongling based on High-Throughput Sequencing and Metabarcoding trnLkopschi) in Taohongling based on High-Throughput Sequencing and Metabarcoding trnL","authors":"Yongtao Xu","doi":"10.17582/journal.pjz/20220629000640","DOIUrl":"https://doi.org/10.17582/journal.pjz/20220629000640","url":null,"abstract":"","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67562428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Insight into the Phylogenetic Position of Capreolus capreolus, the European Roe Deer, (Mammalia: Cervidae) from Turkey using Mitochondrial Genes","authors":"Perinçek Seçkinozan Şeker","doi":"10.17582/journal.pjz/20220621070609","DOIUrl":"https://doi.org/10.17582/journal.pjz/20220621070609","url":null,"abstract":"For contributing to a better understanding of the phylogenetic position and evolutionary history of Turkish roe deer, mitochondrial DNA sequence data belonging to a population from Artvin, Turkey were introduced by this study. The cytochrome b (cyt b ) and cytochrome c oxidase subunit I (COI) genes of mitochondrial DNA belonging to the four samples of Capreolus capreolus were partially sequenced and analyzed alongside the sequences obtained from GenBank. The cyt b and COI data sets included sequences that were 1059 and 646 bp long, respectively. The Turkish roe deer population in Artvin had a relatively low nucleotide diversity and high haplotype diversity. A total of two haplotypes were identified for cyt b , while three haplotypes were identified for COI. Of the haplotypes detected, two cyt b and two COI haplotypes were specific to Turkey. Molecular phylogenetic analyses; Maximum Likelihood (ML), Bayesian Inference (BI), and Network revealed topologies and evolutionary networks which were not definitely resolved. Consequently, Turkish roe deer haplotypes were intertwined with the roe deer haplotypes from Central Europe instead of forming an independent evolutionary clade, and were also, found genetically close to Central European haplotypes. According to the evolutionary dating analysis, the divergence of C. capreolus populations in Europe started at the beginning of the Pleistocene (approximately 2.72 mya). This analysis also demonstrated that the divergence of Turkish roe deer began at approximately 0.58 mya. Given the existence of, shared haplotypes, the relative positions of Turkish roe deer haplotypes in the topologies of the ML and BI phylogenetic trees and evolutionary networks, and the times of evolutionary divergence events within this species; the following results may be inferred: (1) roe deer individuals with the same genotype as those in Europe can also be found in areas further east, such as Turkey; (2) these could be considered to be representatives, which may have contributed to the formation and shaping processes of the known admixed genetic structure of European roe deer populations in Europe; and finally, (3) the roe deer populations may have probably entered Turkey from Europe when evolutionary divergence times based on BI analysis are considered.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67562527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification of Nine Gastropod Species with Three New Records using Cytochrome Oxidase Sub-Unit 1 Marker","authors":"Qurat-ul-Ain Ijaz","doi":"10.17582/journal.pjz/20220701070727","DOIUrl":"https://doi.org/10.17582/journal.pjz/20220701070727","url":null,"abstract":"Qurat-ul-Ain Ijaz1, Amna Sulaman1, Dost Muhammad Baloch2, Muhammad Shafi2, Faiz Muhammad1* and Shahnaz Rashid1 1Center of Excellence in Marine Biology, University of Karachi, Karachi-75270, Pakistan 2Lasbella University of Agriculture, Water and Marine Sciences, Lasbella, Uthal Article Information Received 01 July 2022 Revised 15 August 2022 Accepted 14 September 2022 Available online 27 April 2023 (early access)","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67563212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Nutritional Content on Artificially Supplied Seed Utilization of Wintering Birds in a Mixed Forest, South Korea","authors":"Hyun-Su Hwang","doi":"10.17582/journal.pjz/20220928070901","DOIUrl":"https://doi.org/10.17582/journal.pjz/20220928070901","url":null,"abstract":"1Team of Specific Protected Area Research, National Institute of Ecology, Seocheon 33657, South Korea 2School of Bioresource and Bioscience, Chung-Ang University, Ansung, South Korea 3Animal Resources Division, National Institute of Biological Resources, Incheon 22689, South Korea. Article Information Received 28 September 2022 Revised 19 October 2022 Accepted 09 November 2022 Available online10 January 2023 (early access)","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67567553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Value of Procalcitonin Combined with C-Reactive Protein Assay in the Early Clinical Diagnosis of Bloodstream Infection","authors":"Cheng-Hong Chen","doi":"10.17582/journal.pjz/20221011021005","DOIUrl":"https://doi.org/10.17582/journal.pjz/20221011021005","url":null,"abstract":".","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67567828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Haemato-biochemical Changes and Various Risk Factors Associated with Bovine Coronavirus Infection in Cattle Calves","authors":"S. Abbas","doi":"10.17582/journal.pjz/20220930120931","DOIUrl":"https://doi.org/10.17582/journal.pjz/20220930120931","url":null,"abstract":"Bovine coronavirus (BCoV) is a major viral pathogen linked to respiratory and enteric problems in newborn calves. The goal of this study is to look into the molecular occurrence, haemato-biochemical changes, and risk factors associated with the occurrence of BCoV infection in cattle calves at different dairy farms and small households in the district of Jhelum, Pakistan. From July 2020 to June 2021, 200 faecal samples were collected from newborn cattle calves exhibiting symptoms of diarrhoea and dysentery. S&C Biotech Bovine Coronavirus Antigen Rapid Test Kits were used to screen samples, which were then subjected to RT-PCR for molecular characterization. For the haemato-biochemical analysis, blood was taken from calves infected with BCoV. A questionnaire was used to collect information about the risk factors associated with the occurrence of BCoV infection. BCoV infection was found in 3.5% (7/200) of calves using diagnostic screening kits and 3.0% (6/200) of calves using RT-PCR. Breed, age, sex, vomiting, previous history of diarrhoea, body conditions, food type, colostrum feeding, deworming history, living environment, seasonal variation, and cohabitation with other animals were all assumed risk factors for BCoV infection ( p< 0.05). There was a significant ( p< 0.001) decrease in MCV (fl) and a significant ( p< 0.006) increase in TLC (1x10 3 /cm 3 ) on haematological analysis in BCoV-infected calves. TEC (1x10 6 /cm 3 ), WBC (1x10 6 /cm 3 ), RBC (1x10 6 /cm 3 ), and Hct% were significantly ( p< 0.05) higher. Similarly, infected calves had a non-significant ( p< 0.090) increase in K (mEq/L) and a significant ( p< 0.000) decrease in Na (mEq/L), Ca (mmol/L), Cu (mol/L), and Fe (mol/L) on biochemical analysis. It was concluded that the occurrence of BCoV infection was predisposed by assumed risk factors, and haemato-biochemical alterations were observed in BCoV infected calves.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"32 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67568168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ameliorative Effect of Dietary Glutamine on Antioxidant Capacity of Broiler Chicks During Starter Phase Under Cold Stress","authors":"Wenfang Lou","doi":"10.17582/journal.pjz/20221013091007","DOIUrl":"https://doi.org/10.17582/journal.pjz/20221013091007","url":null,"abstract":"1Department of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang, China 2College of Animal Science and Technology, Jiangxi Agriculture University, Jiangxi Province Key Laboratory of Animal Nutrition, Engineering Research Center of Nutritional Feed Development, Nanchang, China 3College of Animal Science, Anhui Science and Technology University, Fengyang, China 4College of Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67568378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Population Data and Internal Validation of the 21 Short Tandem Repeat Loci in Turkish Population","authors":"Aatif Ahmad Khan","doi":"10.17582/journal.pjz/20221013061003","DOIUrl":"https://doi.org/10.17582/journal.pjz/20221013061003","url":null,"abstract":"","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67568707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}