Bioinformatics Analysis of Promoter Methylation and Clinical Significance of Tumor Suppressor Gene HOXA5 in Oral Cancer

IF 0.5 4区生物学 Q4 ZOOLOGY

Pakistan Journal of Zoology Pub Date : 2023-01-01 DOI:10.17582/journal.pjz/20230803165114

Haichao Wang

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引用次数: 0

Abstract

The HOXA5 gene is an important tumor suppressor gene, which has the function of regulating cell cycle, proliferation, and differentiation. Down regulation or inactivation of HOXA5 expression occurs in a variety of malignant tumors such as oral cancer (OC) and breast cancer. The promoter region of HOXA5 gene is often methylated, and it is of great value to explore the relationship between its methylation status and gene expression level. The objective of this study was to explore the promoter methylation status of tumor suppressor gene HOXA5 in OC. HOXA5 mRNA expression was detected by RT-PCR. OC CAL-27, H357, and HSC-3 cells were treated with different concentrations of 5-aza-deoxyeytidine to analyze the inhibitory effect of 5-aza-deoxyeytidine on methylation. The methylation status of HOXA5 gene promoter was closely related to OC. 5Aza-CdR could induce the expression of HOXA5 mRNA in OC cells, promote cell apoptosis, and inhibit the growth and spread of tumor cells. The promoter region of HOXA5 gene is hypermethylated in OC. It was concluded that 5Aza-CdR can inhibit the activity of DNA methyltransferase, restore the expression of HOXA5 gene, and inhibit the proliferation and spread of cancer cells.

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口腔癌抑癌基因HOXA5启动子甲基化的生物信息学分析及临床意义

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来源期刊

Pakistan Journal of Zoology 生物-动物学

CiteScore

1.10

自引率

16.70%

发文量

306

审稿时长

4.5 months

期刊介绍： Pakistan Journal of Zoology (Pakistan J. Zool.) publishes original articles in English on all aspects of animal life. Generally these articles will be in, or related to one of the following subject areas: Physiology, Cell Biology, Molecular Biology, Genetics, Bioinformatics, Toxicology, Forensic Science, Developmental Biology, Entomology, Parasitology, Microbiology, Biotechnology, Pathology, Palaeontology. Taxonomy, Environmental Biology, Wildlife, Fisheries, Vertebrate and Invertebrate Morphology. Additionally, the journal considers research on health and clinical studies. Short communications are regularly considered, however, uninvited review articles, first records/reports of known species, case reports/studies and survey reports are not published in Pakistan Journal of Zoology.