Parasitology最新文献_第5页

A generalist vector-transmitted parasite exhibits population genetic structure among host genera. 一种多面手病媒传播寄生虫在宿主属间表现出种群遗传结构。

IF 2.1 3区医学

Parasitology Pub Date : 2025-01-21 DOI: 10.1017/S0031182024001641

Vincenzo A Ellis, Mélanie Duc, Arif Ciloglu, Olof Hellgren, Staffan Bensch

{"title":"A generalist vector-transmitted parasite exhibits population genetic structure among host genera.","authors":"Vincenzo A Ellis, Mélanie Duc, Arif Ciloglu, Olof Hellgren, Staffan Bensch","doi":"10.1017/S0031182024001641","DOIUrl":"10.1017/S0031182024001641","url":null,"abstract":"Generalist parasites experience selective pressures from the various host species they infect. However, it is unclear if parasite transmission among host species precludes the establishment of host-specific adaptations and population genetic structure. We assessed the population genetic structure of the vector-transmitted avian haemosporidian parasite Haemoproteus majoris (lineage WW2; n = 34 infections) in a single site in southern Sweden among 10 of its host species. The 2 best-sampled host genera were Phylloscopus (2 species, n = 15 infections) and Sylvia (4 species, n = 15). We designed a sequence capture protocol to isolate 1.13 Mbp (ca. 5%) of the parasite genome and identified 1399 variable sites among the sequenced infections. In a principal components analysis, infections of Phylloscopus and Sylvia species mostly separated along the first 2 principal components. Sites with the highest FST values between the genera were found in genes that have mostly not been implicated in infection pathways, but several sites code for amino acid changes. An analysis of molecular variance confirmed significant variation among host genera, but not among host species within genera. The distribution of Tajima's D among sequenced loci was negatively skewed, plausibly reflecting a history of bottleneck followed by population expansion. Tajima's D was lower in infections of Phylloscopus than Sylvia, plausibly because WW2 began infecting Phylloscopus hosts after it was already a parasite of Sylvia hosts. Our results provide evidence of vector-transmitted parasite population differentiation among host species in a single location. Future work should focus on identifying the mechanisms underlying this genetic population structure.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-10"},"PeriodicalIF":2.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sinistroporomonorchis bolini n. sp. (Trematoda: Monorchiidae) from the Ocellated killifish, Floridichthys polyommus (Cyprinodontidae) in the Yucatán Peninsula, Mexico based on an integrative taxonomy analysis. 墨西哥Yucatán半岛多角鳉鱼（Cyprinodontidae）中Sinistroporomonorchis bolini n. sp.（滴虫目：滴虫科）的综合分类分析。

IF 2.1 3区医学

Parasitology Pub Date : 2025-01-21 DOI: 10.1017/S0031182025000010

Leopoldo Andrade-Gómez, Juan F Espínola-Novelo, Brenda Solórzano-García, Gerardo Pérez-Ponce de León

{"title":"Sinistroporomonorchis bolini n. sp. (Trematoda: Monorchiidae) from the Ocellated killifish, Floridichthys polyommus (Cyprinodontidae) in the Yucatán Peninsula, Mexico based on an integrative taxonomy analysis.","authors":"Leopoldo Andrade-Gómez, Juan F Espínola-Novelo, Brenda Solórzano-García, Gerardo Pérez-Ponce de León","doi":"10.1017/S0031182025000010","DOIUrl":"10.1017/S0031182025000010","url":null,"abstract":"Members of Sinistroporomonorchis Wee, Cutmore, Pérez-del-Olmo & Cribb, 2020 represent a small group of trematodes belonging to the Monorchiidae Odhner, 1911 with 5 species described from mugilid hosts. Specimens consistent with the generic concept of Sinistroporomonorchis were obtained from Floridichthys polyommus (Cyprinodontidae); most of them were juveniles from 4 localities within the Yucatán Peninsula. After a detailed morphological examination including scanning electron microscopy images and a principal component analysis, the specimens collected represented a new species, Sinistroporomonorchis bolini n. sp. The new species can be differentiated by the presence of an overall large pharynx including the proportion of pharynx width to oral sucker width, a uterus arranged in 2 main lateral fields, and by presenting robust caeca. In addition, sequences of the 28S of large subunit of nuclear ribosomal RNA and cox1 of the mitochondrial DNA were obtained. Phylogenetic trees inferred from each dataset, placed all the specimens in a monophyletic clade, confirming that the isolates belonged to the same species. The new species is the sixth described for the genus Sinistroporomonorchis, the fifth described from the Yucatán Peninsula and the first described from a non-mugilid host.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-9"},"PeriodicalIF":2.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ivermectin vs moxidectin for treating Strongyloides stercoralis infection: a systematic review. 伊维菌素与莫西菌素治疗粪线虫感染的系统综述。

IF 2.1 3区医学

Parasitology Pub Date : 2025-01-17 DOI: 10.1017/S0031182024001215

Cesar Henriquez-Camacho, Jose A Pérez-Molina, Dora Buonfrate, Paola Rodari, Eduardo Gotuzzo, Benilde Luengo, María Nieves Plana

{"title":"Ivermectin vs moxidectin for treating Strongyloides stercoralis infection: a systematic review.","authors":"Cesar Henriquez-Camacho, Jose A Pérez-Molina, Dora Buonfrate, Paola Rodari, Eduardo Gotuzzo, Benilde Luengo, María Nieves Plana","doi":"10.1017/S0031182024001215","DOIUrl":"https://doi.org/10.1017/S0031182024001215","url":null,"abstract":"The aim was to assess the efficacy of ivermectin vs moxidectin for treating Strongyloides stercoralis infection. Ovid MEDLINE, Embase and Web of Science databases were searched for studies comparing ivermectin and moxidectin from inception to February 2024. The outcomes: elimination of infection or parasitological cure, mortality and serious adverse events. We calculated odds ratios (ORs) with 95% confidence intervals (CIs) for dichotomous data. Heterogeneity was assessed using Chi2 test for statistical heterogeneity and results of the I2 statistic. Two trials met the inclusion criteria that included 821 adult participants. Both studies were conducted in southeast Asia (Cambodia and Laos). Neither trial included immunocompromised patients. The mean age of the participants ranged from 40 to 45 years old, with a similar distribution of males and females. For all participants, S. stercoralis infection was confirmed by Baermann method. The evidence was moderate for parasitological cure rate. Certainty was downgraded by 1 level because of imprecision. Moxidectin was not inferior to ivermectin: OR 0.67, 95% CI 0.36–1.25 (P = 0.21), I2 = 0%, 821 participants. No deaths were reported in either trial. One trial reported mild adverse events. In total, 153/726 (21%) participants had an adverse event. The most reported symptoms were abdominal pain and headache. There is evidence for moderate quality that moxidectin is non-inferior to, and as safe as ivermectin; however, more high-quality and well-designed trials are needed. For patients with some underlying immunosuppressive disorder, or in patients who are very young or very old, current data are insufficient to be recommended.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-7"},"PeriodicalIF":2.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Helminth diversity of nutria (Myocastor coypus) across the Morava basin in the Czech Republic. 捷克共和国莫拉瓦盆地秧鸡（Myocastor coypus）螺旋虫的多样性。

IF 2.1 3区医学

Parasitology Pub Date : 2024-12-20 DOI: 10.1017/S0031182024001628

Michal Benovics, Eva Nosková, Anna Klimešová, Lucie Škorpíková, Ema Jaššová, Jakub Drimaj, Jan Slováček, Ondřej Mikulka

{"title":"Helminth diversity of nutria (Myocastor coypus) across the Morava basin in the Czech Republic.","authors":"Michal Benovics, Eva Nosková, Anna Klimešová, Lucie Škorpíková, Ema Jaššová, Jakub Drimaj, Jan Slováček, Ondřej Mikulka","doi":"10.1017/S0031182024001628","DOIUrl":"10.1017/S0031182024001628","url":null,"abstract":"The nutria was introduced to Europe from South America and kept for the fur industry. This semiaquatic rodent became a well-established species in the Czech Republic; however, it still poses a significant threat to the native fauna, not only as a natural competitor but also as a vector of non-indigenous parasites. Our research aimed to investigate the diversity of endoparasitic helminths in nutria, with a particular focus on assessing the risk posed by helminth species with zoonotic potential. A total of 46 nutria cadavers were collected at 8 locations in the Morava River basin and examined using standard parasitological post-mortem procedures. Additionally, coprological and molecular methods were used to identify the parasites. The presence of 6 helminth species was revealed. The highest prevalence was observed for Strongyloides myopotami (78.3%) and Trichuris myocastoris (37.0%), both of which are host-specific nematodes of nutria. Only 2 trematode taxa were recorded (Echinostoma sp. and a representative of the family Psilostomidae). The presence of alveolar hydatid cysts of Echinococcus multilocularis in the livers of 5 nutria specimens was also recorded. Herein, we provide novel molecular data for each parasite species collected, which is valuable for future phylogenetic analyses. Our findings also demonstrate that nutria in the Czech Republic serve as a carrier of helminths with zoonotic potential, particularly E. multilocularis and S. myopotami. Although the nutria is a relatively new species in local fauna, its synanthropic behaviour raises concerns about potential threats to human health, underscoring the importance of exercising caution when handling these animals.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-11"},"PeriodicalIF":2.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiology and transmission patterns of Cryptosporidium spp., and Giardia duodenalis within a One Health framework in rural areas of Eastern Algeria. 阿尔及利亚东部农村地区“同一个健康”框架下隐孢子虫和十二指肠贾第虫的流行病学和传播模式

IF 2.1 3区医学

Parasitology Pub Date : 2024-12-18 DOI: 10.1017/S0031182024001616

Sadiya Maxamhud, Nassiba Reghaissia, AbdElKarim Laatamna, Eleni Gentekaki, Anastasios D Tsaousis

{"title":"Epidemiology and transmission patterns of Cryptosporidium spp., and Giardia duodenalis within a One Health framework in rural areas of Eastern Algeria.","authors":"Sadiya Maxamhud, Nassiba Reghaissia, AbdElKarim Laatamna, Eleni Gentekaki, Anastasios D Tsaousis","doi":"10.1017/S0031182024001616","DOIUrl":"10.1017/S0031182024001616","url":null,"abstract":"Gastrointestinal infections constitute a significant global health concern, particularly in tropical and subtropical regions, caused by various pathogens. Among these, Cryptosporidium spp. and Giardia duodenalis are noteworthy due to their zoonotic potential. In Algeria, molecular epidemiological data on cryptosporidiosis and giardiasis are limited. To fill this gap, the present study aimed to examine the transmission dynamics of Cryptosporidium spp., and Giardia duodenalis in various households. A total of 216 samples were collected from the rural Guelma and Souk Ahras provinces, located in the eastern part of Algeria. These included human and animal faeces, as well as water and soil samples. DNA was extracted, followed by nested PCR targeting the SSU rRNA gene to detect Cryptosporidium spp., while the gp60 gene was amplified for subtyping. Detection of G. duodenalis was performed by qPCR targeting the SSU rRNA gene, followed by amplification of tpi, bg and gdh genes for genotyping and subtyping. Several Cryptosporidium species, including C. bovis, C. ryanae, C. andersoni and C. parvum, were identified in human, animal and environmental samples. The zoonotic C. parvum subtype IIaA17G2R1 was detected in human, animal and soil samples. Giardia duodenalis assemblage B was detected in a human sample, while assemblage E was found in cattle and sheep. The current investigation underscores the importance of the One Health approach in addressing issues related to intestinal parasites, highlighting the need for improved surveillance and control measures in rural settings.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-10"},"PeriodicalIF":2.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide association study analysis of single nucleotide variants in L. infantum associated with IL-6 inflammatory response in visceral leishmaniasis. 内脏利什曼病婴儿乳杆菌单核苷酸变异与IL-6炎症反应相关的全基因组关联研究分析。

IF 2.1 3区医学

Parasitology Pub Date : 2024-12-13 DOI: 10.1017/S0031182024001598

Amanda Miranda da Silva, Kátia Silene Sousa Carvalho, Caio Andrey Bezerra Januário, Raquel Gomes de Sena Carneiro Caldas, Bianka Lopes da Silva Paulino, Débora Cavalcante Braz, Dorcas Lamounier Costa, Gabriel da Luz Wallau, Wilson Jose da Silva Junior, Carlos Henrique Nery Costa

{"title":"Genome-wide association study analysis of single nucleotide variants in L. infantum associated with IL-6 inflammatory response in visceral leishmaniasis.","authors":"Amanda Miranda da Silva, Kátia Silene Sousa Carvalho, Caio Andrey Bezerra Januário, Raquel Gomes de Sena Carneiro Caldas, Bianka Lopes da Silva Paulino, Débora Cavalcante Braz, Dorcas Lamounier Costa, Gabriel da Luz Wallau, Wilson Jose da Silva Junior, Carlos Henrique Nery Costa","doi":"10.1017/S0031182024001598","DOIUrl":"10.1017/S0031182024001598","url":null,"abstract":"Elevated levels of IL-6 in plasma are associated with the severity of visceral leishmaniasis (VL). The clinical manifestations of VL vary among patients, influenced by host factors and the virulence of the Leishmania infantum parasite. Considering that severe VL may result from an exaggerated inflammatory response, this study investigated whether IL-6 could serve as a biomarker to identify pro-inflammatory virulence factors. We conducted a genome-wide association study (GWAS) analysis on L. infantum isolates from patients with VL, whose IL-6 concentrations were measured. The analysis revealed that the relationship between IL-6 levels and clinical outcomes (survival vs mortality) had an area under the curve (AUC) of 0.67 (95% CI 0.52–0.81). A cut-off of 391.7 pg mL−1 for IL-6 was established to conduct a logistic regression analysis. We identified 10 029 single nucleotide variants (SNVs) across 62 genomes, resulting in 6,948 SNVs after filtering, of which 6,341 are located in protein-coding regions. The association analysis with PLINK identified 722 variants, of which 35 showed significant associations, with odds ratios ≥3.3, primarily in coding regions. These findings demonstrate that IL-6 levels tended to be associated with the fatal outcome of VL and highlight 35 novel genetic variants that could serve as potential biomarkers for prognosis. Further research into the biological role of these variants may lead to new therapeutic targets and improve the clinical management of VL, especially in identifying high-risk patients.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-9"},"PeriodicalIF":2.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repurposing statins for the treatment of larval cestodiases: in silico evaluation of statin-HMG-CoA reductase interactions and assessment of statin effects on a cestode model. 将他汀类药物重新用于治疗幼虫绦虫病：他汀类药物-HMG-CoA 还原酶相互作用的硅学评估以及他汀类药物对绦虫模型影响的评估。

IF 2.1 3区医学

Parasitology Pub Date : 2024-12-13 DOI: 10.1017/S0031182024001586

Marina Monteiro Guedes, Jeferson Camargo de Lima, Jéssica Andrade Paes, María Del Pilar Cevasco Contreras, Ana María Celentano, Arnaldo Zaha, Karina Mariante Monteiro, Mara Cecilia Rosenzvit, Henrique Bunselmeyer Ferreira

引用次数: 0

Variation of functional diversity structure measured as combined species dominance, functional diversity, and functional redundancy in two taxa of ectoparasitic arthropods at two spatial scales: host-associated, ecological, and geographic effects. 寄主相关效应、生态效应和地理效应两个空间尺度下，以物种优势度、功能多样性和功能冗余为衡量指标的两个异寄生节肢动物类群功能多样性结构的变化

IF 2.1 3区医学

Parasitology Pub Date : 2024-12-12 DOI: 10.1017/S0031182024001483

Boris R Krasnov, Michal Stanko, Maxim V Vinarski, Natalia P Korallo-Vinarskaya, Irina S Khokhlova

{"title":"Variation of functional diversity structure measured as combined species dominance, functional diversity, and functional redundancy in two taxa of ectoparasitic arthropods at two spatial scales: host-associated, ecological, and geographic effects.","authors":"Boris R Krasnov, Michal Stanko, Maxim V Vinarski, Natalia P Korallo-Vinarskaya, Irina S Khokhlova","doi":"10.1017/S0031182024001483","DOIUrl":"https://doi.org/10.1017/S0031182024001483","url":null,"abstract":"The functional diversity structure of a community can be represented as a combination of three additive components (species dominance D, functional redundancy R, and functional diversity Q) (DRQ approach in which different facets of functional differences between species are considered simultaneously). We applied this concept to assemblages of fleas and gamasid mites parasitic on small mammals at continental (across regions of the Palearctic) and regional (across sampling sites in Slovakia) scales and asked: What are the relative effects of host species, biome/habitat type, and geographic locality on the DRQ composition of a parasite assemblage? At the continental scale, regions were partitioned according to predominant biome or geographic position in a continental section. At the regional scale, sampling sites were partitioned according to habitat type or geographic locality. We tested for differences in the functional diversity structure (measured as the DRQ composition) of an ectoparasite assemblage (a) within a host species between biomes/habitat types or continental sections/localities and (b) between host species within a biome/habitat type or a continental section/locality. At both scales, the functional diversity structure of both flea and mite assemblages differed mainly between host species within a biome/habitat or geographic regions/locations, whereas differences in the DRQ composition between biomes/habitats or geographic regions/locations were only detected in a few host species. We compare our results with the results of earlier studies and conclude that the DRQ approach has an advantage over a single diversity metric and allows a better understanding of spatial variation in different facets of ectoparasite diversity.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-10"},"PeriodicalIF":2.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and serodiagnostic efficacy of a novel echinococcosis-specific recombinant fusion antigen rAgB8/1-Em18-Eg95. 新型包虫病特异性重组融合抗原rAgB8/1-Em18-Eg95的表达及血清诊断效果

IF 2.1 3区医学

Parasitology Pub Date : 2024-12-03 DOI: 10.1017/S0031182024001173

Yang Xianwei, Wang Tao, Chen Yin, Wang Wentao

{"title":"Expression and serodiagnostic efficacy of a novel echinococcosis-specific recombinant fusion antigen rAgB8/1-Em18-Eg95.","authors":"Yang Xianwei, Wang Tao, Chen Yin, Wang Wentao","doi":"10.1017/S0031182024001173","DOIUrl":"https://doi.org/10.1017/S0031182024001173","url":null,"abstract":"Echinococcosis lacks sensitive serological diagnostic tools. The echinococcosis-specific antigens Eg95, AgB8/1 and the Em18 gene sequences were fused and expressed as the novel recombinant antigens rAgB8/1-Em18-Eg95 (T3) and rEm18-Eg95 (T2), used for the diagnosis of hydatid disease, prepared into an enzyme-linked immunosorbent reaction (ELISA) kit, and evaluated for their serological diagnostic value. The relative molecular weight of the T3 protein was 88.1 kDa, the purified concentration was 1.5 mg mL−1, and the purity was 80%. The relative molecular weight of T2 protein was 79.9 kDa, the total protein concentration was 0.5 mg mL−1, and the purity was less than 50%. The overall coincidence rate of T2 protein was low, and it was impossible to distinguish between negative and positive sera. The T3 antigen was coated at 1.0 μg mL−1, the cutoff value was 0.5271, and the serum dilution ratio was 1:400. A T3 ELISA kits (96 tests) was constructed to detect the serum of 272 clinically and pathologically confirmed cases. The sensitivity of T3 was 93.8%, and the specificity was 83.3%. The parasite cross-reaction was 30%. Satisfactorily, the Pearson correlation coefficient between the T3 OD value and lesion diameter was 0.707, showing a strong correlation. T3 exhibits better antigenicity than T2, and the prepared T3 ELISA diagnostic kits reached the laboratory diagnostic level of a commercial kits. T3 can distinguish human cystic echinococcosis (CE) and alveolar echinococcosis (AE) more significantly and predict the diameter of lesions according to the OD value, which provides practical value for drug or surgical efficacy.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-8"},"PeriodicalIF":2.1,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preclinical evaluation of immunogenicity and protective efficacy of a recombinant chimeric protein vaccine against visceral leishmaniasis. 内脏利什曼病重组嵌合蛋白疫苗免疫原性和保护效力的临床前评估。

IF 2.1 3区医学

Parasitology Pub Date : 2024-11-28 DOI: 10.1017/S0031182024001240

Daniela P Lage, Danniele L Vale, Fabiana A G Maia, Vívian T Martins, Marcela G P Silva, Nathalia C Galvani, Mariana M Cardoso, Gabriel J L Moreira, Eduarda M Sombrio, Camila S Freitas, Breno L Pimenta, Karolina O M Falcão, Saulo S G Dias, Raquel S Bandeira, Isabela A G Pereira, Grasiele S V Tavares, Antônio L Teixeira, Miguel A Chávez-Fumagalli, Bruno M Roatt, Ricardo A Machado-de-Ávila, Eduardo A F Coelho

{"title":"Preclinical evaluation of immunogenicity and protective efficacy of a recombinant chimeric protein vaccine against visceral leishmaniasis.","authors":"Daniela P Lage, Danniele L Vale, Fabiana A G Maia, Vívian T Martins, Marcela G P Silva, Nathalia C Galvani, Mariana M Cardoso, Gabriel J L Moreira, Eduarda M Sombrio, Camila S Freitas, Breno L Pimenta, Karolina O M Falcão, Saulo S G Dias, Raquel S Bandeira, Isabela A G Pereira, Grasiele S V Tavares, Antônio L Teixeira, Miguel A Chávez-Fumagalli, Bruno M Roatt, Ricardo A Machado-de-Ávila, Eduardo A F Coelho","doi":"10.1017/S0031182024001240","DOIUrl":"https://doi.org/10.1017/S0031182024001240","url":null,"abstract":"Visceral leishmaniasis (VL) is a tropical disease that can be fatal if acute and untreated. Diagnosis is difficult, the treatment is toxic and prophylactic vaccines do not exist. Leishmania parasites express hundreds of proteins and several of them are relevant for the host's immune system. In this context, in the present study, 10 specific T-cell epitopes from 5 parasite proteins, which were identified by antibodies in VL patients’ sera, were selected and used to construct a gene codifying the new chimeric protein called rCHI. The rCHI vaccine was developed and thoroughly evaluated for its potential effectiveness against Leishmania infantum infection. We used monophosphoryl lipid A (MPLA) and polymeric micelles (Mic) as adjuvant and/or delivery system. The results demonstrated that both rCHI/MPLA and rCHI/Mic significantly stimulate an antileishmanial Th1-type cellular response, with higher production of IFN-γ, TNF-α, IL-12 and nitrite in vaccinated animals, and this response was sustained after challenge. In addition, these mice significantly reduced the parasitism in internal organs and increased the production of IgG2a isotype antibodies. In vivo and in vitro toxicity showed that rCHI is safe for the mammalians, and the recombinant protein also induced in vitro lymphoproliferative response and production of Th1-type cytokines by human cells, which were collected from healthy subjects and treated VL patients. These data suggest rCHI plus MPLA or micelles could be considered as a vaccine candidate against VL.","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1-13"},"PeriodicalIF":2.1,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142740134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0