Pharmaceutical Methods最新文献_第9页

Simultaneous Spectrophotometric Estimation of Candesartan Cilexetil and Hydrochlorothiazidein Tablet Dosage Form 坎地沙坦西蕾蒂酯和氢氯噻嗪片剂型的同时分光光度测定

Pharmaceutical Methods Pub Date : 2015-12-01 DOI: 10.5530/PHM.2015.6.23

M. M. Annapurna, J. Kumar

{"title":"Simultaneous Spectrophotometric Estimation of Candesartan Cilexetil and Hydrochlorothiazidein Tablet Dosage Form","authors":"M. M. Annapurna, J. Kumar","doi":"10.5530/PHM.2015.6.23","DOIUrl":"https://doi.org/10.5530/PHM.2015.6.23","url":null,"abstract":"A new simple derivative spectrophotometric method has been developed for the simultaneous determination of Candesartan Cilexetil and Hydrochlorothiazide in tablet dosage forms. Candesartan antagonizes the effect of angiotensin II (vasoconstriction and aldosterone secretion) by blocking the angiotensin II receptor in vascular smooth muscle and the adrenal gland and decrease the blood pressure whereas Hydrochlorothiazide increases chloride, sodium and water excretion by interfering with transport of sodium ions across renal tubular epithelium. And there by show the diuretic action. The first derivative method is based on the measurement of absorbance of one drug at the zero crossing point of another drug. Candesartan Cilexetil and Hydrochlorothiazide were determined at two different wavelengths 222.69 (zero crossing point of Hydrochlorothiazide) and 254.63 nm (zero crossing point of Candesartan Cilexetil) from the derivative spectra respectively. The methods hows linearity over the concentration range 0.5-50 and 0.1-50 µg/ml for Candesartan Cilexetil and Hydrochlorothiazide respectively in phosphate buffer. The proposed method was validated and can be used for routine analysis of combined tablet dosage forms containing Candesartan Cilexetil and Hydrochlorothiazide. Key words: Candesartan Cilexetil, Derivative spectroscopy, Hydrochlorothiazide, Simultaneous determination.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"33 1","pages":"148-151"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81255406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Simultaneous Derivative Spectrophotometric Determination of Lornoxicam and Paracetamol in Tablet Dosage Forms 同时导数分光光度法测定片剂中氯诺昔康和扑热息痛的含量

Pharmaceutical Methods Pub Date : 2015-12-01 DOI: 10.5530/PHM.2015.6.19

Bharampuram Akhil, R. K. Chaitanya, B. Venkatesh, M. M. Annapurna

引用次数: 3

Analytical Stress Degradation Studies of Cabazitaxel (A Semi synthetic Natural Taxoid) using Liquid Chromatography 半合成天然类紫杉醇卡巴他赛的液相色谱分析应力降解研究

Pharmaceutical Methods Pub Date : 2015-12-01 DOI: 10.5530/PHM.2015.6.21

B. Venkatesh, M. M. Annapurna, K. Pramadvara

引用次数: 2

New Derivative and Differential Spectrophotometric Methods for the Determination of Pterostilbene-An Antioxidant 新的导数和微分分光光度法测定紫檀芪- a抗氧化剂

Pharmaceutical Methods Pub Date : 2015-12-01 DOI: 10.5530/PHM.2015.6.22

M. M. Annapurna, J. Kumar

{"title":"New Derivative and Differential Spectrophotometric Methods for the Determination of Pterostilbene-An Antioxidant","authors":"M. M. Annapurna, J. Kumar","doi":"10.5530/PHM.2015.6.22","DOIUrl":"https://doi.org/10.5530/PHM.2015.6.22","url":null,"abstract":"Pterostilbene found in blueberries and Pterocarpus marsupium heartwood is structurally similar to resveratrol. It is used for the treatment of cancer, diabetes and fungal infections. Three spectrophotometric methods have been developed for the determination of Pterostilbene. Two first derivative methods and one difference spectrophotometric method were attempted. Two first derivative spectroscopic methods were developed in sodium hydroxide (Method A) and methanol (Method B) and differential spectroscopic method (Method C) was developed using borate buffer and sodium hydroxide. Linearity was observed over the concentration range 0.1-25 and 1-25 μg/ml with linear regression equations y = 0.0043x-0.0003 (R 2 =0.9997) and y=0.0037x - 0.0007 (R 2 =0.9997) for method A and B respectively whereas for method C the linearity was followed over the concentration range 0.1-20 μg/ml with linear regression equation y=0.1124x - 0.0016 (R 2 =0.9999). The three methods were validated and can be applied for the determination of Pterostilbene pharmaceutical formulations. Key words: Derivative spectroscopy, Differential spectroscopy, Pterostilbene, Validation.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"137 1","pages":"143-147"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86289017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Development of Dispersible Self-microemulsifying Tablet of Atorvastatin 阿托伐他汀分散自微乳化片的研制

Pharmaceutical Methods Pub Date : 2015-10-20 DOI: 10.5530/PHM.2015.6.2

K. Midha, M. Nagpal, G. Aggarwal, T. G. Singh

{"title":"Development of Dispersible Self-microemulsifying Tablet of Atorvastatin","authors":"K. Midha, M. Nagpal, G. Aggarwal, T. G. Singh","doi":"10.5530/PHM.2015.6.2","DOIUrl":"https://doi.org/10.5530/PHM.2015.6.2","url":null,"abstract":"Aim: The aim of this study was to develop dispersible self-microemulsifying (SMEDDS) tablet of atorvastatin for promoting its solubility and thus its oral bioavailability. Materials and Methods: The liquid SMEDDS were prepared by water titration method using oil, surfactant and co-surfactant and converted into solid- SMEDDS (S-SMEDDS) by adsorption on solid carriers (Neusilin US2). The S-SMEDDS were blended with sodium starch glycolate (disintegrant) and tablet excipients and compressed into tablets that were dispersible and self-microemulsifying in nature. All these formulations were assessed for various physicochemical parameters viz. weight variation, hardness, friability, disintegration test. In vitro studies of pure drug, SMEDDS, S-SMEDDS and dispersible SME-tablets were carried out. Results: Pure drug released only 29.84 ± 0.16% upto 60 minutes and all the SMEDDS formulations (i.e. SMEDDS. S-SMEDDS and dispersible SME-tablets) released 100% of drug in comparatively lesser time. Formulations containing atorvastatin, 30% oleic acid, 65% tween 80 and 5% co-surfactant came out to show the best results in in vitro studies. But, FB1 (tablet) was considered to be the best since it released 100% drug in 35 min and also has advantages over SMEDDS and S-SMEDDS in terms of stability and patient compliance. Conclusion: The study revealed the potential use of dispersible SMEDDS tablet for the oral delivery of hydrophobic drugs, such as atorvastatin. Key words: Atorvastatin, Bioavailability, Phase diagram, SMEDDS.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"635 1","pages":"9"},"PeriodicalIF":0.0,"publicationDate":"2015-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86807076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

In vivo study of pH Dependent and Enzymatically Triggered Colon Targeted Tinidazole Microspheres pH依赖性和酶促结肠靶向替硝唑微球的体内研究

Pharmaceutical Methods Pub Date : 2015-10-20 DOI: 10.5530/PHM.2015.6.6

Poonam Kushwaha

引用次数: 0

Analysis of 3-Methyl-4-nitrophenol, A Major Metabolite of Fenitrothion, In Mice Urine Using HPLC 用高效液相色谱法分析小鼠尿液中非硝硫磷主要代谢物3-甲基-4-硝基苯酚的含量

Pharmaceutical Methods Pub Date : 2015-10-20 DOI: 10.5530/PHM.2015.6.4

A. S. Surur, Ahungena Alemayyehu, T. Esho

{"title":"Analysis of 3-Methyl-4-nitrophenol, A Major Metabolite of Fenitrothion, In Mice Urine Using HPLC","authors":"A. S. Surur, Ahungena Alemayyehu, T. Esho","doi":"10.5530/PHM.2015.6.4","DOIUrl":"https://doi.org/10.5530/PHM.2015.6.4","url":null,"abstract":"Introduction: 3-methyl-4-nitrophenol is a major metabolite of fenitrothion and is considered as a potential risk to public health owning to its genotoxicity and carcinogenicity. Objective: To develop and validate an HPLC method for the quantification of 3-methyl-4-nitrophenol in urine. Methods: A sample of mice urine was collected following the administration of fenitrothion solution through oral gavage and subsequently, 3-methyl-4-nitrophenol was extracted using a simple liquid-liquid extraction method. The newly developed method uses acetonitrile: water 60:40 mobile phase at 270 nm with a flow rate of 1 mL/min HPLC condition. An optimization to a previously used extraction method was made by using ethyl acetate instead of acetonitrile to avoid extraction of water-soluble components of urine other than 3-methyl- 4-nitrophenol. The developed method was validated using the ICH guideline for the validation of analytical procedures. Accordingly, the selectivity, linearity, limit of detection and quantification, accuracy, precision, extraction efficacy, robustness and stability of the method was determined. Results: All of the validation parameters showed that the method is valid for the determination of 3-methyl-4-nitrophenol in urine sample. Using this method it was possible to detect low concentration of 3-methyl-4-nitrophenol in urine samples down to 0.87 μg/mL. Conclusion: The sample was analyzed with this validated method and the respective concentration of the 3-methyl-4-nitrophenol was found to be 31.11 μg/mL. Accordingly, 10.37% of the total fenitrothion administered to mice was found metabolized into 3-methyl-4-nitrophenol. The HPLC retention time for 3-methyl-4-nitrophenol was 2.81 minutes. Key words: Fenitrothion, 3-methyl-4-nitrophenol, HPLC, Metabolites, Method development, Validation.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"22 1","pages":"35-41"},"PeriodicalIF":0.0,"publicationDate":"2015-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88423288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spectrophotometric and High Performance Liquid Chromatographic Determination of Ofloxacin and Azithromycin in Pharmaceutical Tablets 分光光度-高效液相色谱法测定片剂中氧氟沙星和阿奇霉素的含量

Pharmaceutical Methods Pub Date : 2015-10-20 DOI: 10.5530/PHM.2015.6.3

M. Hinge, Nikita A. Desai, Rajvi J. Mahida, H. Dalvadi

{"title":"Spectrophotometric and High Performance Liquid Chromatographic Determination of Ofloxacin and Azithromycin in Pharmaceutical Tablets","authors":"M. Hinge, Nikita A. Desai, Rajvi J. Mahida, H. Dalvadi","doi":"10.5530/PHM.2015.6.3","DOIUrl":"https://doi.org/10.5530/PHM.2015.6.3","url":null,"abstract":"Absorbance ratio spectrophotometric method and high performance liquid chromatographic method were developed for the determination of the Ofloxacin and Azithromycin in tablet dosage form. In UV-Spectrophotometric method, estimation of Ofloxacin and Azithromycin was carried out at 230.60 nm (isoabsorptive point) and 288.60 nm (λ max of Ofloxacin) for absorbance ratio method. Calibration curves were linear in the range of 4–20 μg/ml for Ofloxacin and 5-25 μg/ml Azithromycin for absorbance ratio method. Correlation coefficient found to be close to 0.9995 for both the drugs. Accuracy for both the drugs was in the range of 98-101%. A simple liquid chromatographic assay has been developed for the determination of Ofloxacin and Azithromycin. A C 18 (250 mm × 4.6 mm, 5 μ) column was used with a mobile phase consisting of Phosphate buffer: Methanol ( pH adjusted to 5.0 with ortho phosphoric acid) at a flow rate of 1.0 ml min -1 . Quantitation was achieved with UV detection at 215 nm based on the peak height ratios. Beer’s law was obeyed in a concentration range of 12-28 μg ml -1 for Ofloxacin and 15-35 μg ml -1 for Azithromycin and the regression line equation was derived with a correlation coefficient of 0.9970 and 0.9980 for Ofloxacin and Azithromycin respectively. The validity of the methods was further confirmed using the standard addition method. The proposed procedures were successfully applied to the determination of Ofloxacin and Azithromycin in bulk and tablet form, with high percentage of recovery, good accuracy and precision. Key words: Absorbance ratio method, Azithromycin, HPLC, Ofloxacin, Tablets.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"63 1","pages":"26-34"},"PeriodicalIF":0.0,"publicationDate":"2015-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74319986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Local Drug Delivery Systems as an Adjunct to Cure Periodontitis- The Novel Dental Applicant 局部药物输送系统作为治疗牙周炎的辅助手段-新的牙科应用

Pharmaceutical Methods Pub Date : 2015-10-20 DOI: 10.5530/PHM.2015.6.1

S. Garg

{"title":"Local Drug Delivery Systems as an Adjunct to Cure Periodontitis- The Novel Dental Applicant","authors":"S. Garg","doi":"10.5530/PHM.2015.6.1","DOIUrl":"https://doi.org/10.5530/PHM.2015.6.1","url":null,"abstract":"Periodontitis is a complex disease that involves the loss of attachment around teeth resulting from action of microorganisms and the response of the host to these organisms. The basic treatment of chronic periodontitis is a mechanical debridement of periodontal pockets by Scaling and root planing (SRP) in combination with efficient plaque control. The effectiveness of mechanical debridement of plaque and repeated topical and systemic administration of antibacterial agents are limited due to the lack of accessibility to periodontopathic organisms in the periodontal pocket. The introduction of a slow release and subgingival delivery of tetracycline has changed the rationale from a mechanical treatment towards a combined therapy for full mouth/sites disinfection. Various antibiotics, antiseptics and resorbable carriers are now proposed as Local drug delivery systems with similar targets to arrest disease progression. The dosage forms include fibers, film, injectable systems, liposomal system, micro or nanoparticle based systems, some of which are degradable while others are not and need to be removed at the termination of the treatment. These products provide a long-term, effective treatment at the site of infection at much smaller doses. This article reviews the current status of controlled local delivery their usefulness, as well as the advancement of these systems in the treatment of periodontitis. \u0000 \u0000Key wors: Chlorhexidine, Doxycycline, Local drug delivery systems, Minocycline, Metronidazole, Tetracyclines.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"32 11","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2015-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91498517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Comparative Stability Evaluation of Marketed Paracetamol IV Formulations in India 印度市售扑热息痛IV制剂的比较稳定性评价

Pharmaceutical Methods Pub Date : 2015-10-20 DOI: 10.5530/PHM.2015.6.5

Santosh Chandgadkar, Sreenivasulu Dasari, R. Sundaram

{"title":"Comparative Stability Evaluation of Marketed Paracetamol IV Formulations in India","authors":"Santosh Chandgadkar, Sreenivasulu Dasari, R. Sundaram","doi":"10.5530/PHM.2015.6.5","DOIUrl":"https://doi.org/10.5530/PHM.2015.6.5","url":null,"abstract":"Paracetamol is a well-established drug widely used around the world for analgesic and antipyretic purposes in oral solid dosage forms. While paracetamol is stable in solid dosage form, intravenous formulations have significant stability related issues, as the drug is susceptible to hydrolysis and oxidation in aqueous media. Formation of hydrolytic product can be well controlled by adjusting the pH of formulation. However, control of oxidation of the drug during manufacturing and packaging is a challenge, as it requires sophisticated operational controls to remove dissolved oxygen and/or addition of compatible anti-oxidant. Seven commercially available paracetamol intravenous formulations from the Indian market were evaluated for their oxidative degradation potential. The study was performed in two tiers: i) to compare formation of oxidative products in selected stress degradation conditions; and ii) to determine presence of cysteine, an anti-oxidant excipient. Firstly, all formulations were subjected to thermal stress condition at 80oC for 7 days and oxidative stress condition with 2,2' -azobis (2-amidino-propane) dihydrochloride (AAPH) at 40oC for 48 hours. Of the seven formulations tested, one formulation (PerfalganTM, Manufactured by BMS) showed absence of oxidative products after both stress conditions. For tier II studies, a sensitive HILIC-MS/MS method was developed on API 4000 (AB Sciex) for the determination of cysteine presence. Only PerfalganTM showed presence of cysteine. Thus, PerfalganTM can be expected to have better stability compared to other marketed formulations. Overall, it is concluded that presence of an anti-oxidant in intravenous formulation could give additional advantage over stability of paracetamol. Key words: HILIC, LC-MS, Oxidative stress, Paracetamol, stability.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"47 1","pages":"42-47"},"PeriodicalIF":0.0,"publicationDate":"2015-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90889016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1