Periodicum Biologorum最新文献

Tissue optical clearing methods for microscopy: A review of their application in neuroscience 显微组织光学清除方法:在神经科学中的应用综述

4区生物学

Periodicum Biologorum Pub Date : 2023-10-25 DOI: 10.18054/pb.v125i1-2.24860

Dominik Hamer, Daniela Petrinec, Monika Berecki, Anton Glasnović, Srećko Gajović

引用次数: 0

A young researcher’s guide to three-dimensional fluorescence microscopy of living cells 年轻研究者的活细胞三维荧光显微镜指南

4区生物学

Periodicum Biologorum Pub Date : 2023-10-25 DOI: 10.18054/pb.v125i1-2.25140

Vedrana Filić, Igor Weber

引用次数: 0

Morphology of extrafloral nectaries of Ailanthus altissima (Mill.) Swingle (Simaroubaceae) 花外花蜜的形态Swingle (Simaroubaceae)

4区生物学

Periodicum Biologorum Pub Date : 2023-10-25 DOI: 10.18054/pb.v125i1-2.24852

Danijela Poljuha, Mirela Uzelac, Tea Zubin Ferri, Danijela Damijanić, Mihaela Šimunić, Hrvoje Korovljević, Tim Weber, Barbara Sladonja

{"title":"Morphology of extrafloral nectaries of Ailanthus altissima (Mill.) Swingle (Simaroubaceae)","authors":"Danijela Poljuha, Mirela Uzelac, Tea Zubin Ferri, Danijela Damijanić, Mihaela Šimunić, Hrvoje Korovljević, Tim Weber, Barbara Sladonja","doi":"10.18054/pb.v125i1-2.24852","DOIUrl":"https://doi.org/10.18054/pb.v125i1-2.24852","url":null,"abstract":"Background and Purpose: Ailanthus altissima (Mill.) Swingle (Tree of Heaven) is a highly invasive, widespread, and widely investigated plant species native to the broader area of China. Extrafloral nectaries are major components of the A. altissima secretory system, but the knowledge of their morphology and role in the tree's physiology is limited. This research aims to explore the morphology of extrafloral nectaries, compare it to previous findings, and discuss their possible role and function. Materials and Methods: Extrafloral nectaries on leaves have been monitored through different phases of leaf development, from June to August 2015 and 2022. The nectaries' morphology was investigated using Zeiss Axioscope 5 and BOECO BSZ-405 light microscopes, and FEG QUANTA 250 FEI scanning electron microscope, operating at 7 kV and pressure of 60–100 Pa, without samples pretreatment. Results: Our investigation revealed the absence of earlier reported pores or ducts on the top of the glands. However, it supports one of the first, systematical investigations of A. altissima extrafloral nectaries conducted in Croatia a century ago, which was until recently forgotten by history. We evaluated our findings against prior theories and assessed the nectaries' potential role and purpose in disposing surplus sugars. Conclusions: The nectar in A. altissima is not secreted via an opening on the leaves but rather through epidermal tissue tearing.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135218737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of dendrin, neurofilament and glial fibrillary acidic protein in the brain of the dogfish Scyliorhinus canicula L. 角鲨脑内树突蛋白、神经丝和胶质原纤维酸性蛋白的表达。

4区生物学

Periodicum Biologorum Pub Date : 2023-10-25 DOI: 10.18054/pb.v125i1-2.23766

Ivana Restović, Marko Vučemilo, Mia Obad, Nives Kević, Nela Kelam, Anita Racetin, Ivana Bočina

{"title":"Expression of dendrin, neurofilament and glial fibrillary acidic protein in the brain of the dogfish Scyliorhinus canicula L.","authors":"Ivana Restović, Marko Vučemilo, Mia Obad, Nives Kević, Nela Kelam, Anita Racetin, Ivana Bočina","doi":"10.18054/pb.v125i1-2.23766","DOIUrl":"https://doi.org/10.18054/pb.v125i1-2.23766","url":null,"abstract":"Background and purpose: Dendrin is a brain and renal protein that is supposed to be involved in cytoskeletal modifications at the synapse and a part of the slit diaphragm and podocytes. Here, we aimed to investigate dendrin expression in dogfish brain since this newly discovered protein was never reported in fish. We compared the expression of dendrin to those of glial (GFAP) and neuronal (NF) proteins, which have already been described in the dogfish brain. Materials and methods: Histological and immunofluorescent techniques were performed on tissue samples. The obtained data were statistically analyzed. Results: Our results have shown that dendrin is expressed in all observed parts of the dogfish brain. In the forebrain, both observed parts (telencephalon and olfactory lobes) expressed dendrin. Regarding the percentage area of dendrin expression, it is expressed more in olfactory lobes than in the telencephalon. Compared with GFAP and NF expression, the expression of dendrin significantly differs in both parts of the forebrain. The highest dendrin expression was noticed in the midbrain. In dogfish midbrain, the difference in expression of dendrin in comparison to those of GFAP and NF was even more significant. The percentage area of dendrin expression in the hindbrain (cerebellum and medulla oblongata) was smaller than those in the forebrain and midbrain, contrary the percentage area of intermediate filaments GFAP and NF were significantly higher. Conclusion: These results are the first report on dendrin expression in the dogfish brain opening the path for future studies on its role and function.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135218734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retro is the new modern: Contemporary application of gold impregnation staining on brain cryosections for digital image analysis 复古是新的现代:当代应用金浸渍染色脑冷冻切片用于数字图像分析

4区生物学

Periodicum Biologorum Pub Date : 2023-10-25 DOI: 10.18054/pb.v125i1-2.23747

Edi Rođak, Milorad Zjalić, Sara Cibok, Sven Viland, Marija Heffer, Robert Grgac, Tatjana Belovari, Nada Oršolić, Nikola Bijelić

{"title":"Retro is the new modern: Contemporary application of gold impregnation staining on brain cryosections for digital image analysis","authors":"Edi Rođak, Milorad Zjalić, Sara Cibok, Sven Viland, Marija Heffer, Robert Grgac, Tatjana Belovari, Nada Oršolić, Nikola Bijelić","doi":"10.18054/pb.v125i1-2.23747","DOIUrl":"https://doi.org/10.18054/pb.v125i1-2.23747","url":null,"abstract":"Background and purpose: Since the times of Golgi and Cajal, impregnation with gold or silver has been used to visualize microscopic details of the nervous tissue. Although immunohistochemistry has largely replaced impregnation techniques, they are still used, and there is a growing interest in combining them with modern image analysis methods for quantitative studies in neuroscience. The aim of this research was to modify the gold chloride impregnation method published by Schmued to improve consistency of staining, to be adequate for digital image analysis. Materials and methods: Brains of 8 six-month-old female Wistar rats were fixed in 4% PFA, cryoprotected in sucrose and flash‑frozen in liquid nitrogen. Neighboring sets of coronal sections were chosen for gold impregnation, Nissl staining and MAP2 immunohistochemistry. Whole-slide images and images of specific regions were taken for analysis. Results: Myelinated fibers were stained dark reddish to brown on goldstained sections, and other tissue was yellowish, which gave an excellent contrast for digital image analysis. Gold staining was consistent in all regions, and no major artifacts were noticed. When compared to Nissl and MAP2, only myelinated structures were stained with gold impregnation. Conclusions: Modified gold impregnation method is an alternative that’s on par with traditional myelin staining methods. The new, modified gold impregnation method gives a consistent and reproducible staining suitable for digital image analysis. It can be useful in morphometric evaluation of nervous tissue and investigation of neuropathological changes in nervous tissue, especially for quantitative studies.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135218736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SEM and TEM investigations of materials at the Centre for Electron Microscopy and Microanalysis (CEMM) 电子显微镜和微量分析中心(CEMM)材料的SEM和TEM研究

4区生物学

Periodicum Biologorum Pub Date : 2023-10-25 DOI: 10.18054/pb.v125i1-2.25775

Miran Čeh, Jitka Hreščak, Sandra Drev

引用次数: 0

Celebrating 40 years of the Croatian Microscopy Society 庆祝克罗地亚显微镜学会成立40周年

4区生物学

Periodicum Biologorum Pub Date : 2023-10-25 DOI: 10.18054/pb.v125i1-2.26468

Danijela Poljuha, Goran Kovačević

引用次数: 0

Attendance of extracurricular activities in the field of natural sciences and the attractiveness of the content offered for extracurricular activities in biology in elementary schools 参加自然科学领域的课外活动和小学生物课外活动内容的吸引力

4区生物学

Periodicum Biologorum Pub Date : 2023-10-25 DOI: 10.18054/pb.v125i1-2.24077

Damir Sirovina, Daniela Petrinec, Petra Tramontana, Goran Kovačević

{"title":"Attendance of extracurricular activities in the field of natural sciences and the attractiveness of the content offered for extracurricular activities in biology in elementary schools","authors":"Damir Sirovina, Daniela Petrinec, Petra Tramontana, Goran Kovačević","doi":"10.18054/pb.v125i1-2.24077","DOIUrl":"https://doi.org/10.18054/pb.v125i1-2.24077","url":null,"abstract":"Background and purpose: For many years, students’ interest in the field of natural sciences has declined, which consequently results in decreasing numbers of professional staff in the mentioned field. The aim of this study was to examine the attendance of extracurricular activities in the field of natural sciences by elementary school students (age 13 and 14) in Zagreb and whether the teachers' expectations are in line with the students' preferences for the content offered as part of extracurricular activities in biology. Material and methods: Using an anonymous online survey (Google Forms), data were collected on participation in extracurricular activities in the field of natural sciences and the attractiveness of the content of extracurricular activities. The respondents were 90 students in the 7th grade and 46 teachers of biology in Zagreb, 2020. Results: Participation in extracurricular activities in the field of natural sciences among the surveyed students was very low. Students were attracted to content that includes simple practical work in the field of biology, including microscopy, but the perception of their interest by teachers is not in accordance with the real interests of the students. Conclusion: The results of this research show that the share of the science field in extracurricular activities is low, and that the surveyed teachers are not aware of the students' wishes regarding the content offered in extracurricular activities. The conducted research can be a good basis for organizing and planning extracurricular activities in elementary school.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135218728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of atomic force microscopy for characterization of model membranes and cells 使用原子力显微镜表征模型膜和细胞

4区生物学

Periodicum Biologorum Pub Date : 2023-10-25 DOI: 10.18054/pb.v125i1-2.24080

Anja Sadžak, Lucija Mandić, Suzana Šegota, Vida Strasser

{"title":"Use of atomic force microscopy for characterization of model membranes and cells","authors":"Anja Sadžak, Lucija Mandić, Suzana Šegota, Vida Strasser","doi":"10.18054/pb.v125i1-2.24080","DOIUrl":"https://doi.org/10.18054/pb.v125i1-2.24080","url":null,"abstract":"Background: To provide a fundamental understanding of the potential and use of atomic force microscopy (AFM) in medicine and the life sciences, this work presents a thorough description of imaging and non-imaging atomic force microscopy modes for characterizing model membranes and cells at the nanoscale. Methods: The imaging and non-imaging AFM modes are described with examples in terms of the characterization of topographic, morphological, and nanomechanical sample properties. Results: AFM imaging of supported lipid bilayers (SLBs) revealed the effects of temperature and medium composition on SLB topography in the gel and fluid phases, and on the bilayer thickness. Non-imaging AFM showed the strengthening of the SLB in both phases by the ion binding process.Imaging of neuronal and neuroblastoma cells with and without treatment revealed morphological changes in shape, volume, roughness, and Feret dimension. Non-imaging AFM showed the change in cell elasticity induced by the treatment with H2O2 with and without quercetin and by the treatment with copper and myricetin. The measurements of cells elasticity revealed a reorganization of the cytoskeleton and filament structures. Conclusions: Diverse applications of imaging and non-imaging AFM can provide important information about the underlying processes in biologically relevant systems. AFM, as a complementary technique to other biomedical methods, allows screening and monitoring of physiological changes at the nanoscale.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135218735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural and immunofluorescence features of the epidermal cells and its secretory granules in the amphioxus Branchiostoma lanceolatum L. 文昌鱼表皮细胞及其分泌颗粒的超微结构和免疫荧光特征。

4区生物学

Periodicum Biologorum Pub Date : 2023-10-25 DOI: 10.18054/pb.v125i1-2.23764

Nives Kević, Ivana Restović, Ivana Bočina

{"title":"Ultrastructural and immunofluorescence features of the epidermal cells and its secretory granules in the amphioxus Branchiostoma lanceolatum L.","authors":"Nives Kević, Ivana Restović, Ivana Bočina","doi":"10.18054/pb.v125i1-2.23764","DOIUrl":"https://doi.org/10.18054/pb.v125i1-2.23764","url":null,"abstract":"Background and purpose: In the vertebrate family, the epidermis of terrestrial animals is keratinized while in aquatic forms and amphibians can produce mucus. Amphioxus, a cephalochordate, is an important animal model in the study of chordate phylogeny. Major cytoskeletal and extracellular matrix proteins in epidermal cells of amphioxus (Branchiostoma lanceolatum L.) were investigated using transmission electron microscopy and immunofluorescence. Materials and methods: Amphioxus specimens were fresh caught in the Adriatic Sea. Tissue samples were fixed and prepared for transmission electron microscopy and immunofluorescence techniques. Results: The epidermis consisted of one-layered columnar epithelium. Epithelial cells contained voluminous nucleus, a well-developed supranuclear Golgi apparatus, abundant vesicles and apical secretory vesicles surrounded by cytokeratin filaments. A single row of smaller vesicles also appeared close to the basal cell membrane. Strong positive immunolocalization of hyaluronic acid and collagen type I was observed in the apical domain of the plasma membrane of amphioxus, respectively. The positive signal of hyaluronan and collagen proteins referred to the secretory granules in the apical domain of the epidermal cell. Strong positive labelling of aggrecan was seen around the cell nucleus, deep below the apical domain, suggesting that aggrecan is not located in the secretory granules. A strong signal of the cytokeratin 10 protein was seen throughout the cytoplasm of the epidermal cells. Conclusion: Due to its morphology and histochemical composition, epidermal cells of amphioxus may contribute to course surface protection and the mucous layer in amphioxus may serve as a physical barrier between the body and its environment.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135218729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0