文昌鱼表皮细胞及其分泌颗粒的超微结构和免疫荧光特征。

Pub Date : 2023-10-25 DOI:10.18054/pb.v125i1-2.23764
Nives Kević, Ivana Restović, Ivana Bočina
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引用次数: 0

摘要

背景与目的:在脊椎动物家族中,陆生动物的表皮是角化的,而在水生动物和两栖动物中,表皮可以产生粘液。文昌鱼是头脊索动物的一种,是脊索动物系统发育研究的重要动物模型。采用透射电镜和免疫荧光技术对文昌鱼(Branchiostoma lanceolatum L.)表皮细胞的主要细胞骨架蛋白和细胞外基质蛋白进行了研究。材料与方法:在亚得里亚海新鲜捕获文昌鱼标本。组织样品固定并准备用于透射电子显微镜和免疫荧光技术。结果:表皮为单层柱状上皮。上皮细胞含有体积庞大的细胞核、发育良好的核上高尔基体、丰富的囊泡和被细胞角蛋白丝包围的顶端分泌囊泡。在基底细胞膜附近也可见一单排小泡。文昌鱼质膜顶端区分别可见到强阳性免疫定位的透明质酸和ⅰ型胶原蛋白。透明质酸和胶原蛋白的阳性信号是指表皮细胞顶端的分泌颗粒。在细胞核周围,在顶域以下深处可见聚集蛋白的强烈阳性标记,表明聚集蛋白不在分泌颗粒中。在整个表皮细胞的细胞质中可见强烈的细胞角蛋白10信号。结论:文昌鱼的表皮细胞由于其形态和组织化学组成,可能具有保护体表的作用,而文昌鱼的黏液层可能是身体与环境之间的物理屏障。
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Ultrastructural and immunofluorescence features of the epidermal cells and its secretory granules in the amphioxus Branchiostoma lanceolatum L.
Background and purpose: In the vertebrate family, the epidermis of terrestrial animals is keratinized while in aquatic forms and amphibians can produce mucus. Amphioxus, a cephalochordate, is an important animal model in the study of chordate phylogeny. Major cytoskeletal and extracellular matrix proteins in epidermal cells of amphioxus (Branchiostoma lanceolatum L.) were investigated using transmission electron microscopy and immunofluorescence. Materials and methods: Amphioxus specimens were fresh caught in the Adriatic Sea. Tissue samples were fixed and prepared for transmission electron microscopy and immunofluorescence techniques. Results: The epidermis consisted of one-layered columnar epithelium. Epithelial cells contained voluminous nucleus, a well-developed supranuclear Golgi apparatus, abundant vesicles and apical secretory vesicles surrounded by cytokeratin filaments. A single row of smaller vesicles also appeared close to the basal cell membrane. Strong positive immunolocalization of hyaluronic acid and collagen type I was observed in the apical domain of the plasma membrane of amphioxus, respectively. The positive signal of hyaluronan and collagen proteins referred to the secretory granules in the apical domain of the epidermal cell. Strong positive labelling of aggrecan was seen around the cell nucleus, deep below the apical domain, suggesting that aggrecan is not located in the secretory granules. A strong signal of the cytokeratin 10 protein was seen throughout the cytoplasm of the epidermal cells. Conclusion: Due to its morphology and histochemical composition, epidermal cells of amphioxus may contribute to course surface protection and the mucous layer in amphioxus may serve as a physical barrier between the body and its environment.
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