Parasite Immunology最新文献_第2页

Variants of NLRP3 Protein in Haemonchus contortus Infected Sheep: Impact on Immune Cell Responsiveness to LPS In Vitro. 受血包虫病感染的绵羊体内 NLRP3 蛋白的变异：对体外免疫细胞对 LPS 反应性的影响

IF 1.4 4区医学

Parasite Immunology Pub Date : 2024-06-01 DOI: 10.1111/pim.13054

Denzel Middleton, Kelly Hanlon, Scott P Greiner, Scott A Bowdridge

{"title":"Variants of NLRP3 Protein in Haemonchus contortus Infected Sheep: Impact on Immune Cell Responsiveness to LPS In Vitro.","authors":"Denzel Middleton, Kelly Hanlon, Scott P Greiner, Scott A Bowdridge","doi":"10.1111/pim.13054","DOIUrl":"https://doi.org/10.1111/pim.13054","url":null,"abstract":"Pathogen recognition is an essential component to achieve the desired outcome of host protection. Nod-like receptor pyrin containing domain 3 (NLRP3) is a cytoplasmic pattern recognition receptor (PRR) with a wide array of agonists, such as PAMPs, DAMPs, ATP, bacterial product and viral products. Stimulation of the NLRP3 inflammasome results in proteolytic activation of IL-1β and IL-18, cell pyroptosis and classically, the induction of proinflammatory responses. St. Croix (STC) sheep have resistance traits exhibiting the appropriate T-helper type 2 immune response ensuing protection during helminth parasitic infection whereas parasite-susceptible Suffolk (SUF) sheep have an impaired response resulting in parasite establishment and adverse symptoms. The objective of these experiments was to determine if NLRP3 protein in H. contortus-infected SUF sheep was defective using the classical activation pathway of NLRP3 inflammasome. Peripheral blood mononuclear cells (PBMCs) derived from H. contortus-infected STC and SUF sheep were isolated from whole blood and treated (MCC950 treatment for 2 h followed by LPS treatment for 3 h, 1400 W treatment for 2 h followed by LPS treatment for 3 h, LPS treatment for 3 h or culture media for 3 h). qPCR analysis of LPS-stimulated PBMC revealed an upregulation in inflammatory associated genes IL-1β, TLR4, TNFα and NFκB (p < 0.0001) in STC PBMC and downregulation in IFNγ, IL-6 and iNOS for SUF PBMC. Pharmacological inhibition of iNOS in SUF PBMC resulted in an upregulation in the expression of IFNγ. These preliminary data begin to discover a relationship between NLRP3 activation and TLR4 signalling in PBMC of STC and SUF sheep.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141458664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Receptor for Advanced Glycation End Product (RAGE) Modulates Inflammation During Feeding of the Hard Tick, Haemaphysalis longicornis in Mice. 高级糖化终产物受体（RAGE）调节小鼠摄食硬蜱 Haemaphysalis longicornis 期间的炎症反应。

IF 2.2 4区医学

Parasite Immunology Pub Date : 2024-06-01 DOI: 10.1111/pim.13039

Anisuzzaman, Md Abdul Alim, Makoto Matsubyashi, Md Shahadat Hossain, Sharmin Shahid Labony, Ireen Sultana Shanta, Md Haydar Ali, Yasuhiko Yamamoto, Takeshi Hatta, Naotoshi Tsuji

{"title":"Receptor for Advanced Glycation End Product (RAGE) Modulates Inflammation During Feeding of the Hard Tick, Haemaphysalis longicornis in Mice.","authors":"Anisuzzaman, Md Abdul Alim, Makoto Matsubyashi, Md Shahadat Hossain, Sharmin Shahid Labony, Ireen Sultana Shanta, Md Haydar Ali, Yasuhiko Yamamoto, Takeshi Hatta, Naotoshi Tsuji","doi":"10.1111/pim.13039","DOIUrl":"https://doi.org/10.1111/pim.13039","url":null,"abstract":"Ticks are notorious blood-sucking ectoparasites that affect both humans and animals. They serve as a unique vector of various deadly diseases. Here, we have shown the roles of the receptor for advanced glycation end products (RAGE) during repeated infestations by the tick Haemaphysalis longicornis using RAGE-/- mice. In primary infestation, a large blood pool developed, which was flooded with numerous RBCs, especially during the rapid feeding phase of the tick both in wild-type (wt) and RAGE-/- mice. Very few inflammatory cells were detected around the zones of haemorrhage in the primary infestations. However, the number of inflammatory cells gradually increased in the subsequent tick infestations, and during the third infestations, the number of inflammatory cells reached to the highest level (350.3 ± 16.8 cells/focus). The site of attachment was totally occupied by the inflammatory cells in wt mice, whereas very few cells were detected at the ticks' biting sites in RAGE-/- mice. RAGE was highly expressed during the third infestation in wt mice. In the third infestation, infiltration of CD44+ lymphocytes, eosinophils and expression of S100A8 and S100B significantly increased at the biting sites of ticks in wt, but not in RAGE-/- mice. In addition, peripheral eosinophil counts significantly increased in wt but not in RAGE-/- mice. Taken together, our study revealed that RAGE-mediated inflammation and eosinophils played crucial roles in the tick-induced inflammatory reactions.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141262588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circadian Regulation of Leishmania Parasite Internalisation in Macrophages and Downstream Cellular Events. 巨噬细胞中利什曼病寄生虫内化的昼夜节律调控及下游细胞事件

IF 2.2 4区医学

Parasite Immunology Pub Date : 2024-06-01 DOI: 10.1111/pim.13053

Priscilla Carvalho Cabral, Sophia K Stegeman, Martin Olivier, Nicolas Cermakian

{"title":"Circadian Regulation of Leishmania Parasite Internalisation in Macrophages and Downstream Cellular Events.","authors":"Priscilla Carvalho Cabral, Sophia K Stegeman, Martin Olivier, Nicolas Cermakian","doi":"10.1111/pim.13053","DOIUrl":"https://doi.org/10.1111/pim.13053","url":null,"abstract":"Leishmania spp. parasites use macrophages as a host cell during infection. As a result, macrophages have a dual role: clearing the parasite as well as acting as host cells. Recently, studies have shown that macrophages harbour circadian clocks, which affect many of their functions such as phagocytosis, receptor expression and cytokine release. Interestingly, Leishmania major infection in hosts was also shown to be under circadian control. Therefore, we decided to investigate what underlies the rhythms of L. major infection within macrophages. Using a culture model of infection of bone marrow-derived macrophages with L. major promastigotes, we show that the parasites are internalised into macrophages with a 24-h variation dependent on a functional circadian clock in the cells. This was associated with a variation in the number of parasites per macrophage. The cell surface expression of parasite receptors was not controlled by the cells' circadian clock. In contrast, the expression of the components of the endocytic pathway, EEA1 and LC3b, varied according to the time of infection. This was paralleled by variations in parasite-induced ROS production as well as cytokine tumour necrosis factor α. In summary, we have uncovered a time-dependent regulation of the internalisation of L. major promastigotes in macrophages, controlled by the circadian clock in these cells, as well as subsequent cellular events in the endocytic pathway, intracellular signalling and cytokine production.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141180446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trichinella spiralis Larval Extract as a Biological Anti-Tumor Therapy in a Murine Model of Ehrlich Solid Carcinoma. 螺旋毛旋毛虫幼虫提取物作为艾氏实体癌小鼠模型的生物抗肿瘤疗法

IF 2.2 4区医学

Parasite Immunology Pub Date : 2024-05-01 DOI: 10.1111/pim.13035

Salwa S Younis, Amina M Salama, Dalia A Elmehy, Nehal A Heabah, Hanem M Rabah, Sara H Elakshar, Radwa A Awad, Ghada A Gamea

{"title":"Trichinella spiralis Larval Extract as a Biological Anti-Tumor Therapy in a Murine Model of Ehrlich Solid Carcinoma.","authors":"Salwa S Younis, Amina M Salama, Dalia A Elmehy, Nehal A Heabah, Hanem M Rabah, Sara H Elakshar, Radwa A Awad, Ghada A Gamea","doi":"10.1111/pim.13035","DOIUrl":"https://doi.org/10.1111/pim.13035","url":null,"abstract":"Trichinella spiralis (T. spiralis) is an immunomodulating parasite that can adversely affect tumor growth and extend host lifespan. The aim of this study was to elucidate the mechanisms by which T. spiralis larval antigens achieve this effect using Ehrlich solid carcinoma (ESC) murine model. Assessment was done by histopathological and immunohistochemical analysis of caspase-3, TNF-α, Ki-67 and CD31. Additionally, Bcl2 and Bcl2-associated protein X (Bax) relative gene expression was assessed by molecular analysis for studying the effect of T. spiralis crude larval extract (CLE) antigen on tumor necrosis, apoptosis, cell proliferation and angiogenesis. We found that both T. spiralis infection and CLE caused a decrease in the areas of necrosis in ESC. Moreover, they led to increased apoptosis through activation of caspase-3, up-regulation of pro-apoptotic gene, Bax and down-regulation of anti-apoptotic gene, Bcl2. Also, T. spiralis infection and CLE diminished ESC proliferation, as evidenced by decreasing Ki-67. T. spiralis infection and CLE were able to suppress the development of ESC by inhibiting tumor proliferation, inducing apoptosis and decreasing tumor necrosis, with subsequent decrease in tumor metastasis. T. spiralis CLE antigen may be considered as a promising complementary immunotherapeutic agent in the treatment of cancer.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140868518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Acute Inflammatory Potential of Particles From the Echinococcus granulosus Laminated Layer Is Moderated by Its Calcium Inositol Hexakisphosphate Component. 棘球蚴层压微粒的急性炎症潜能受其肌醇六磷酸钙成分的调节

IF 2.2 4区医学

Parasite Immunology Pub Date : 2024-05-01 DOI: 10.1111/pim.13040

Leticia Grezzi, Carlos González, Álvaro Díaz, Cecilia Casaravilla

{"title":"The Acute Inflammatory Potential of Particles From the Echinococcus granulosus Laminated Layer Is Moderated by Its Calcium Inositol Hexakisphosphate Component.","authors":"Leticia Grezzi, Carlos González, Álvaro Díaz, Cecilia Casaravilla","doi":"10.1111/pim.13040","DOIUrl":"https://doi.org/10.1111/pim.13040","url":null,"abstract":"Cystic echinococcosis is caused by the tissue-dwelling larva (hydatid) of Echinococcus granulosus sensu lato. A salient feature is that this larva is protected by the acellular laminated layer (LL). As the parasite grows, the LL sheds abundant particles that can accumulate in the parasite's vicinity. The potential of LL particles to induce inflammation in vivo has not been specifically analysed. It is not known how each of its two major components, namely highly glycosylated mucins and calcium inositol hexakisphosphate (InsP6) deposits, impacts inflammation induced by the LL as a whole. In this work, we show that LL particles injected intraperitoneally cause infiltration of eosinophils, neutrophils and monocytes/macrophages as well as the disappearance of resident (large peritoneal) macrophages. Strikingly, the absence of calcium InsP6 enhanced the recruitment of all the inflammatory cell types analysed. In contrast, oxidation of the mucin carbohydrates caused decreased recruitment of neutrophils. The carbohydrate-oxidised particles caused cell influx nonetheless, which may be explained by possible receptor-independent effects of LL particles on innate immune cells, as suggested by previous works from our group. In summary, LL particles can induce acute inflammatory cell recruitment partly dependent on its mucin glycans, and this recruitment is attenuated by the calcium InsP6 component.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apolipoprotein E Is Upregulated in Blood and Circulating Monocytes of Indian Patients With Visceral Leishmaniasis. 印度内脏利什曼病患者血液和循环单核细胞中载脂蛋白 E 上调。

IF 2.2 4区医学

Parasite Immunology Pub Date : 2024-05-01 DOI: 10.1111/pim.13036

Gulafsha Kausar, Shashi B Chauhan, Ritirupa Roy, Shashi Kumar, Christian Engwerda, Susanne Nylen, Rajiv Kumar, Mary E Wilson, Shyam Sundar

引用次数: 0

Immunotherapy Combining Mimotopes Selected by Phage Display Plus Amphotericin B Is Effective for Treatment Against Visceral Leishmaniasis. 噬菌体展示法筛选出的拟态物与两性霉素 B相结合的免疫疗法对治疗内脏利什曼病有效

IF 2.2 4区医学

Parasite Immunology Pub Date : 2024-05-01 DOI: 10.1111/pim.13037

Tauane Gonçalves Soyer, Raquel Soares Bandeira Câmara, Isabela Amorim Gonçalves Pereira, Fernanda Fonseca Ramos, Marcelo Moreira de Jesus, Fernanda Ludolf, Guilherme de Paula Costa, Daniela Pagliara Lage, Camila Simões de Freitas, Danniele Luciana Vale, Breno Luiz Pimenta, Vívian Tamietti Martins, Alexsandro Sobreira Galdino, Miguel Angel Chávez-Fumagalli, Bruno Mendes Roatt, Grasiele de Sousa Vieira Tavares, Eduardo Antonio Ferraz Coelho

{"title":"Immunotherapy Combining Mimotopes Selected by Phage Display Plus Amphotericin B Is Effective for Treatment Against Visceral Leishmaniasis.","authors":"Tauane Gonçalves Soyer, Raquel Soares Bandeira Câmara, Isabela Amorim Gonçalves Pereira, Fernanda Fonseca Ramos, Marcelo Moreira de Jesus, Fernanda Ludolf, Guilherme de Paula Costa, Daniela Pagliara Lage, Camila Simões de Freitas, Danniele Luciana Vale, Breno Luiz Pimenta, Vívian Tamietti Martins, Alexsandro Sobreira Galdino, Miguel Angel Chávez-Fumagalli, Bruno Mendes Roatt, Grasiele de Sousa Vieira Tavares, Eduardo Antonio Ferraz Coelho","doi":"10.1111/pim.13037","DOIUrl":"https://doi.org/10.1111/pim.13037","url":null,"abstract":"The treatment for visceral leishmaniasis (VL) causes toxicity in patients, entails high cost and/or leads to the emergence of resistant strains. No human vaccine exists, and diagnosis presents problems related to the sensitivity or specificity of the tests. Here, we tested two phage clones, B1 and D11, which were shown to be protective against Leishmania infantum infection in a murine model as immunotherapeutics to treat mice infected with this parasite species. The phages were used alone or with amphotericin B (AmpB), while other mice received saline, AmpB, a wild-type phage (WTP) or WTP/AmpB. Results showed that the B1/AmpB and D11/AmpB combinations induced polarised Th1-type cellular and humoral responses, which were primed by high levels of parasite-specific IFN-γ, IL-12, TNF-α, nitrite and IgG2a antibodies, which reflected in significant reductions in the parasite load in distinct organs of the animals when analyses were performed 1 and 30 days after the treatments. Reduced organic toxicity was also found in these animals, as compared with the controls. In conclusion, preliminary data suggest the potential of the B1/AmpB and D11/AmpB combinations as immunotherapeutics against L. infantum infection.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140892320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interleukin 17F Gene Polymorphism as a Potential Protective Factor in the Immunopathology of Ocular Toxoplasmosis. 白细胞介素 17F 基因多态性是眼弓形虫病免疫病理学的潜在保护因素

IF 1.4 4区医学

Parasite Immunology Pub Date : 2024-05-01 DOI: 10.1111/pim.13038

Danilo Donizete da Silva, Fábio Batista Frederico, Mariana Previato, Rubens Camargo Siqueira, Claudia Regina Bonini-Domingos, Victor Hugo de Souza, Lilian Castiglioni, Cinara Cássia Brandão, Luiz Carlos de Mattos, Christiane Maria Ayo

{"title":"Interleukin 17F Gene Polymorphism as a Potential Protective Factor in the Immunopathology of Ocular Toxoplasmosis.","authors":"Danilo Donizete da Silva, Fábio Batista Frederico, Mariana Previato, Rubens Camargo Siqueira, Claudia Regina Bonini-Domingos, Victor Hugo de Souza, Lilian Castiglioni, Cinara Cássia Brandão, Luiz Carlos de Mattos, Christiane Maria Ayo","doi":"10.1111/pim.13038","DOIUrl":"10.1111/pim.13038","url":null,"abstract":"Ocular toxoplasmosis (OT) is characterised by intraocular inflammation due to Toxoplasma gondii infection. Studies have found that interleukin 17 (IL-17) plays a central role in the pathology of OT. However, nucleotide variability in IL17 and interleukin 17 receptor (IL17R) genes has not been characterised in OT. As cytokine gene polymorphisms may influence the expression of these molecules, the aim of this study was to verify whether IL17A (rs2275913), IL17F (rs763780), IL17RA (rs4819554) and IL17RC (rs708567) polymorphisms are associated with OT in a Brazilian population. This study enrolled 214 patients seropositive for T. gondii (110 with OT and 104 without) and 107 controls. Polymorphisms were identified by PCR-restriction fragment length polymorphism analysis, validated by DNA sequencing with chi-square and multivariate analyses being used to assess possible associations between polymorphisms and OT. Logistic regression under the dominant model revealed a protection factor against OT of the C mutant allele of the IL17F (rs763780) polymorphism. The T/C-C/C genotypes were significantly more common in patients without OT compared to those with OT (p value = 0.0066) and controls (p value = 0.014). Findings from this study suggest that the IL17F polymorphism may have an influence in the immunopathology of OT in Brazilian individuals.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant CD5 and CD6 Ectodomains Induce Antiparasitic and Immunomodulatory Effects in Secondary Cystic Echinococcosis 重组 CD5 和 CD6 外显子在继发性囊性棘球蚴病中诱导抗寄生虫和免疫调节作用

IF 2.2 4区医学

Parasite Immunology Pub Date : 2024-04-16 DOI: 10.1111/pim.13034

Joaquín García-Luna, Florencia Rivero-Osorio, María Clara González-Porcile, Paula Arbildi, Sebastián Miles, Javier Magnone, María Velasco-De-Andrés, Sylvia Dematteis, Francisco Lozano, Gustavo Mourglia-Ettlin

{"title":"Recombinant CD5 and CD6 Ectodomains Induce Antiparasitic and Immunomodulatory Effects in Secondary Cystic Echinococcosis","authors":"Joaquín García-Luna, Florencia Rivero-Osorio, María Clara González-Porcile, Paula Arbildi, Sebastián Miles, Javier Magnone, María Velasco-De-Andrés, Sylvia Dematteis, Francisco Lozano, Gustavo Mourglia-Ettlin","doi":"10.1111/pim.13034","DOIUrl":"https://doi.org/10.1111/pim.13034","url":null,"abstract":"Scavenger receptors participate in a wide range of biological functions after binding to multiple non-self or altered self-ligands. Among them, CD5 and CD6 are lymphocyte scavenger receptors known to interact with different microbial-associated molecular patterns, and the administration of the recombinant soluble ectodomains of human CD5 (rshCD5) and/or CD6 (rshCD6) has shown therapeutic/prophylactic potential in experimental models of fungal, bacterial and echinococcal infections. The latter is a zoonosis caused by the larval stage of the cestode parasite Echinococcus granulosus sensu lato, which in humans can induce secondary cystic echinococcosis (CE) after the spillage of protoscoleces contained within fertile cysts, either spontaneously or during surgical removal of primary hydatid cysts. Herein, we have analysed the mechanisms behind the significant protection observed in the mouse model of secondary CE following prophylactic administration of rshCD5 or rshCD6. Our results show that both molecules exhibit intrinsic antiparasitic activities in vitro, as well as immunomodulatory functions during early secondary CE, mainly through Th1/Th17 cytokine bias and promotion of peritoneal polyreactive antibodies. These data support the relevance of the parasite components bound by rshCD5 and rshCD6, as well as the potential of their prophylactic administration as a useful strategy to reduce secondary CE in patients.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140602439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the Role of Endoplasmic Reticulum Stress Pathways in Canine Demodicosis 揭示内质网应激途径在犬脱毛症中的作用

IF 2.2 4区医学

Parasite Immunology Pub Date : 2024-04-12 DOI: 10.1111/pim.13033

Pamela A. Kelly, Gillian P. McHugo, Caitriona Scaife, Susan Peters, M. Lynn Stevenson, Jennifer S. McKay, David E. MacHugh, Irene Lara Saez, Rory Breathnach

{"title":"Unveiling the Role of Endoplasmic Reticulum Stress Pathways in Canine Demodicosis","authors":"Pamela A. Kelly, Gillian P. McHugo, Caitriona Scaife, Susan Peters, M. Lynn Stevenson, Jennifer S. McKay, David E. MacHugh, Irene Lara Saez, Rory Breathnach","doi":"10.1111/pim.13033","DOIUrl":"https://doi.org/10.1111/pim.13033","url":null,"abstract":"Canine demodicosis is a prevalent skin disease caused by overpopulation of a commensal species of Demodex mite, yet its precise cause remains unknown. Research suggests that T‐cell exhaustion, increased immunosuppressive cytokines, induction of regulatory T cells and increased expression of immune checkpoint inhibitors may contribute to its pathogenesis. This study aimed to gain a deeper understanding of the molecular changes occurring in canine demodicosis using mass spectrometry and pathway enrichment analysis. The results indicate that endoplasmic reticulum stress promotes canine demodicosis through regulation of three linked signalling pathways: eIF2, mTOR, and eIF4 and p70S6K. These pathways are involved in the modulation of Toll‐like receptors, most notably TLR2, and have been shown to play a role in the pathogenesis of skin diseases in both dogs and humans. Moreover, these pathways are also implicated in the promotion of immunosuppressive M2 phenotype macrophages. Immunohistochemical analysis, utilising common markers of dendritic cells and macrophages, verified the presence of M2 macrophages in canine demodicosis. The proteomic analysis also identified immunological disease, organismal injury and abnormalities and inflammatory response as the most significant underlying diseases and disorders associated with canine demodicosis. This study demonstrates that Demodex mites, through ER stress, unfolded protein response and M2 macrophages contribute to an immunosuppressive microenvironment, thereby assisting in their proliferation.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140583572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0