Parasite Immunology最新文献

Analysis of miR-10a and IFNG Expression Before and After Treatment for Chronic Schistosomiasis mansoni. 慢性曼氏血吸虫病治疗前后miR-10a和IFNG的表达分析

IF 1.4 4区医学

Parasite Immunology Pub Date : 2025-06-01 DOI: 10.1111/pim.70011

Débora Nascimento da Nóbrega, Ana Virgínia Matos Sá Barreto, Roberta Dos Santos Souza, Kleyton Palmeira do Ó, Raul Emídio de Lima, Ana Lúcia Coutinho Domingues, Edmundo Pessoa Lopes, Clarice Neuenschwander Lins de Morais, Elainne Christine de Souza Gomes, Luydson Richardson Silva Vasconcelos

{"title":"Analysis of miR-10a and IFNG Expression Before and After Treatment for Chronic Schistosomiasis mansoni.","authors":"Débora Nascimento da Nóbrega, Ana Virgínia Matos Sá Barreto, Roberta Dos Santos Souza, Kleyton Palmeira do Ó, Raul Emídio de Lima, Ana Lúcia Coutinho Domingues, Edmundo Pessoa Lopes, Clarice Neuenschwander Lins de Morais, Elainne Christine de Souza Gomes, Luydson Richardson Silva Vasconcelos","doi":"10.1111/pim.70011","DOIUrl":"10.1111/pim.70011","url":null,"abstract":"Mir-10a acts in signalling pathways regulating transcription, translation, and RNA-mediated gene silencing, while IFNG acts in the T-cell receptor signalling pathway. Thus, both can be considered potential targets for understanding regulatory processes in chronic inflammation in patients with schistosomiasis. Populations in endemic areas receive mass and indiscriminate praziquantel treatment, and yet patients often have a history of multiple infections. To investigate the regulatory and prognostic capacity of miR-10 and IFNG in patient immunity, we evaluated the expression of miR-10a and IFNG as biomarkers of inflammation and their correlation with praziquantel treatment. miR-10a did not present evidence as a biomarker of inflammation in the therapeutic follow-up in schistosomiasis. However, the levels of IFNG expression were significantly higher before treatment.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":"47 6","pages":"e70011"},"PeriodicalIF":1.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12139366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Potential Role of Monocyte-Derived Macrophages in Preventing Malarial Parasitemic Recrudescence in a Mouse Model. 单核细胞来源的巨噬细胞在小鼠模型中预防疟疾寄生虫复发的潜在作用。

IF 1.4 4区医学

Parasite Immunology Pub Date : 2025-06-01 DOI: 10.1111/pim.70010

Jiaqin Fang, Suilin Chen, Yuanli Gao, Yongling Fan, Shuai Guo, Xiuxiu Li, Hangyu Li, Jian Zhou, Wenyue Xu, Taiping Liu

{"title":"The Potential Role of Monocyte-Derived Macrophages in Preventing Malarial Parasitemic Recrudescence in a Mouse Model.","authors":"Jiaqin Fang, Suilin Chen, Yuanli Gao, Yongling Fan, Shuai Guo, Xiuxiu Li, Hangyu Li, Jian Zhou, Wenyue Xu, Taiping Liu","doi":"10.1111/pim.70010","DOIUrl":"https://doi.org/10.1111/pim.70010","url":null,"abstract":"Frequent recrudescence is responsible for persistent Plasmodium infection after the acute stage. Our previous study demonstrated that phagocytic cells are essential for controlling Plasmodium chabaudi chabaudi AS (P. chabaudi) recrudescence. Nevertheless, the specific type of phagocytic cells involved in controlling P. chabaudi recrudescence, as well as their underlying molecular mechanisms of action, remain elusive. Herein we employ single-cell RNA sequencing (scRNA-seq) to analyse splenic phagocytic cells during both the acute and recrudescent phases of P. chabaudi infection. Using scRNA-seq, we found that monocyte-derived macrophages (MDMs) declined during the acute stage of P. chabaudi blood-stage infection, and then expanded rapidly in the recrudescence stage. The changing trend of MDMs was confirmed by flow cytometry. To explore the potential role of MDMs in controlling parasitemic recrudescence, MDMs were reduced by a low dose of clodronate liposomes (CLs) during the recrudescence stage, which significantly elevated the P. chabaudi parasitemia. Additionally, no significant difference in the proportion of splenic MDMs or classical monocytes (CMs) within the monocyte population was observed between the infected CCR2-/- mice and their control littermates, suggesting that the transition from CMs to MDMs may not occur in this model. The results indicate that MDMs potentially play a protective role in preventing malarial parasitemic recrudescence, offering valuable insights into immune-based interventions against Plasmodium infection and potentially contributing to the prevention of malaria transmission.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":"47 6","pages":"e70010"},"PeriodicalIF":1.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vivo Study on the Anti-Parasitic Effect of Omeprazole on Cryptosporidium parvum in Mice. 奥美拉唑对小隐孢子虫体内抗寄生作用的研究。

IF 1.4 4区医学

Parasite Immunology Pub Date : 2025-06-01 DOI: 10.1111/pim.70012

Mai A Atallah, Samy I El-Kowrany, Omayma K Afifi, Heba H Elkaliny, Salwa S Younis, Ghada A Gamea

{"title":"In Vivo Study on the Anti-Parasitic Effect of Omeprazole on Cryptosporidium parvum in Mice.","authors":"Mai A Atallah, Samy I El-Kowrany, Omayma K Afifi, Heba H Elkaliny, Salwa S Younis, Ghada A Gamea","doi":"10.1111/pim.70012","DOIUrl":"https://doi.org/10.1111/pim.70012","url":null,"abstract":"Cryptosporidiosis is an important enteric disease, causing diarrhoea and malabsorption similar to Rotavirus and targeting young children and immunocompromised individuals, especially AIDS patients. However, there is a lack of fully effective drugs and vaccines against it. This study was done with the aim of investigating the anti-parasitic and anti-inflammatory effects of omeprazole versus nitazoxanide and their combination on Cryptosporidium parvum (C. parvum) infection in immunosuppressed experimental mice. To achieve this aim, histopathological analysis, scanning electron microscopy (SEM), estimation of oocyst shedding and measurement of tumour necrosis factor alpha (TNF-α) levels in sera of mice and the optical density of inducible nitric oxide synthase (iNOS) immunoreactivity in intestinal tissues were performed. Regarding the results, oocyst shedding showed an obvious reduction with omeprazole more than nitazoxanide. Similar results were detected on both histopathological examination (by haematoxylin and eosin and periodic acid-Schiff stains) and SEM with marked improvement in pathology detected in the combination therapy treated group. TNF-α showed reduced levels in the sera of all treated groups indicating a reduction of immunopathology with treatment. Also, the cytoplasmic expression of iNOS in the intestinal epithelium of mice was markedly reduced in all treated groups indicating a reduction of oxidative stress. From these results, omeprazole was found to be superior to nitazoxanide in treating cryptosporidiosis and the use of the two drugs as a combined therapy showed the best results.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":"47 6","pages":"e70012"},"PeriodicalIF":1.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA-Sequencing in Elucidating Immune Responses to Haemonchus contortus Infection in Small Ruminants: Systematic Review. rna测序在阐明小反刍动物对弯曲血蜱感染的免疫反应中的应用：系统综述。

IF 1.4 4区医学

Parasite Immunology Pub Date : 2025-05-01 DOI: 10.1111/pim.70009

Bongeka Sylvia Ndaba, Erika Faber, Munyaradzi Christopher Marufu, Alri Pretorius, Selaelo Ivy Tshilwane

{"title":"RNA-Sequencing in Elucidating Immune Responses to Haemonchus contortus Infection in Small Ruminants: Systematic Review.","authors":"Bongeka Sylvia Ndaba, Erika Faber, Munyaradzi Christopher Marufu, Alri Pretorius, Selaelo Ivy Tshilwane","doi":"10.1111/pim.70009","DOIUrl":"10.1111/pim.70009","url":null,"abstract":"Haemonchus contortus poses a major threat to small ruminant production in subtropical regions worldwide. Unfortunately, there is growing anthelmintic resistance, and the only licensed vaccine has limitations. This paper aimsto review the use of RNA-sequencing in understanding the immune responses of small ruminants to H. contortus infection, focusing on identifying differentially expressed genes and elucidating key immune pathways associated with resistance and susceptibility. This review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Statement guidelines and Population, Intervention, Control, and Outcome framework covering publications from January 2015 to December 2023. RNA-seq identified the activation of key immune pathways, such as Th1/Th2, NK cell, B cell receptor signalling, MAPK, CAMs, and TNF signalling. There was upregulation of a range of PRRs, including TLRs and CLECs, in the resistant sheep, suggesting a crucial role for trained innate immune cells in resistance. However, there are no direct comparisons of TLR and CLEC expression between resistant and susceptible goats. This shows that there is a gap in understanding of the immune response mechanisms in goats. Addressing these knowledge gaps will lead to the development of more effective and sustainable control strategies.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":"47 5","pages":"e70009"},"PeriodicalIF":1.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12079082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of Intestinal Flora in Patients With Schistosoma japonicum Infection Undergoing Splenectomy. 日本血吸虫感染患者脾切除术后肠道菌群特征分析。

IF 1.4 4区医学

Parasite Immunology Pub Date : 2025-05-01 DOI: 10.1111/pim.70008

Chen Zhou, Pengpeng Zhang, Yingzi Ming

{"title":"Characteristics of Intestinal Flora in Patients With Schistosoma japonicum Infection Undergoing Splenectomy.","authors":"Chen Zhou, Pengpeng Zhang, Yingzi Ming","doi":"10.1111/pim.70008","DOIUrl":"https://doi.org/10.1111/pim.70008","url":null,"abstract":"Schistosomiasis japonica is a parasitic disease that seriously endangers human health. Patients with advanced Schistosoma japonicum infection often suffer from cirrhosis and portal hypertension. Splenectomy has been widely used in the treatment of these patients. Previous studies have confirmed that S. japonicum infection is closely related to the gut microbiota, but the impact of splenectomy on the gut microbiota of patients with advanced S. japonicum infection remains unclear. This study used 16sRNA sequencing technology to compare the differences in intestinal flora between patients with advanced S. japonicum infection who underwent splenectomy and non-surgical patients. We focused on the changes in the species composition, diversity and functions of the intestinal flora. Our study shows that dysbiosis of the gut microbiome occurred in patients with advanced S. japonicum infection, including changes in abundance and diversity and the disorder of biological function. The intestinal flora structure, diversity and function of patients who underwent splenectomy were significantly changed compared with those who did not undergo surgery.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":"47 5","pages":"e70008"},"PeriodicalIF":1.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12046944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Hematologic Markers, Procalcitonin and Neopterin in Inflammatory Response in Cattle With Theileria annulata. 血液标志物、降钙素原和新蝶呤素在环孢杆菌感染牛炎症反应中的作用。

IF 1.4 4区医学

Parasite Immunology Pub Date : 2025-04-01 DOI: 10.1111/pim.70007

Ömer Aydın, Mustafa Sinan Aktaş, Emre Eren, Betül Apaydın Yıldırım, İsmail Bolat

{"title":"The Role of Hematologic Markers, Procalcitonin and Neopterin in Inflammatory Response in Cattle With Theileria annulata.","authors":"Ömer Aydın, Mustafa Sinan Aktaş, Emre Eren, Betül Apaydın Yıldırım, İsmail Bolat","doi":"10.1111/pim.70007","DOIUrl":"https://doi.org/10.1111/pim.70007","url":null,"abstract":"The disease named tropical theileriosis is caused by the protozoan pathogen Theileria annulata (T. annulata) transmitted through Hyalomma species ticks. The current study has been designed to determine the haematological, inflammatory and oxidative stress status in cattle naturally infected with T. annulata. The study consisted of two groups: a control group (10 cattle) and a theileriosis group (10 cattle). Compared to the control group, the animals in the theileriosis group exhibited a significant decrease in erythrocyte count, haemoglobin count, haematocrit levels and mean corpuscular haemoglobin concentration, while a notable increase in mean corpuscular volume was observed (p < 0.001; p < 0.01; p < 0.01; p < 0.05; p < 0.05, respectively). However, no significant differences were found between the groups for the other haematological indices (p > 0.05). In terms of oxidative stress markers, the theileriosis group exhibited higher malondialdehyde (MDA) (p < 0.001) and significantly lower superoxide dismutase (SOD) (p < 0.001) levels compared to the control group. No significant difference was observed between the groups in terms of catalase (CAT) levels. Compared to the control group, haptoglobin (Hp) (p < 0.001) and procalcitonin (PCT) (p < 0.001) levels were significantly higher in the theileriosis group, while no significant difference was observed in neopterin levels between the two groups (p > 0.05). In conclusion, significant differences were identified in haematological indices, acute phase response, inflammatory marker levels, and oxidative stress marker levels in theileriosis. Additionally, Hp and PCT levels may be crucial in determining the inflammatory status.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":"47 4","pages":"e70007"},"PeriodicalIF":1.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How Effective Are the Canine Visceral Leishmaniasis Vaccines Currently Being Tested in Dogs? A Systematic Review and Meta-Analysis. 目前在狗身上测试的犬内脏利什曼病疫苗效果如何？系统回顾和荟萃分析。

IF 1.4 4区医学

Parasite Immunology Pub Date : 2025-03-01 DOI: 10.1111/pim.70006

Josiane Aparecida Martiniano de Pádua, Tuane Ferreira Melo, Rafaella Silva Andrade, Marina Martins de Oliveira, Ana Laura Grossi de Oliveira, Andressa Mariana Saldanha-Elias, Ricardo Toshio Fujiwara, Elaine Maria Seles Dorneles, Ana Paula Peconick, Kelly Moura Keller

{"title":"How Effective Are the Canine Visceral Leishmaniasis Vaccines Currently Being Tested in Dogs? A Systematic Review and Meta-Analysis.","authors":"Josiane Aparecida Martiniano de Pádua, Tuane Ferreira Melo, Rafaella Silva Andrade, Marina Martins de Oliveira, Ana Laura Grossi de Oliveira, Andressa Mariana Saldanha-Elias, Ricardo Toshio Fujiwara, Elaine Maria Seles Dorneles, Ana Paula Peconick, Kelly Moura Keller","doi":"10.1111/pim.70006","DOIUrl":"10.1111/pim.70006","url":null,"abstract":"Canine visceral leishmaniasis (CVL) is a zoonotic disease of great worldwide importance and can be prevented by vaccinating seronegative dogs. The objective of the present systematic review and meta-analysis is to verify the efficacy rate of vaccines tested in dogs against CVL or L. infantum infection. We used PRISMA guidelines for this review and Pubmed, Web of Science, Scopus, Cochrane, Scielo and CABI to find studies about vaccines against CVL in dogs. Articles were analysed and grouped according to the antigens used. The risk of bias analysis was performed using SYRCLE's RoB tool and meta-analysis using R Statistical language. The final analysis was conducted using 22 studies that assessed DNA, excreted/secreted proteins and subunit vaccines, involving a total of 92 animals, 96 animals and 78 animals, respectively. Regarding DNA vaccines, the analyses revealed non-significant results in terms of preventing parasite presence in the organs or the onset of clinical signs. However, subunit vaccines demonstrated statistically significant results concerning parasite presence in the organs, but not when it comes to clinical signs. Additionally, there was no statistically significant difference observed in parasite burden in the organs or clinical signs for the excreted/secreted vaccines. The meta-analysis indicated that subunit and excreted/secreted protein vaccines were significantly more effective in preventing parasites in vaccinated animals compared to both DNA-based vaccines and control groups. Heterogeneity among studies is a limitation, emphasising the need for standardised protocols for reliable comparisons.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":"47 3","pages":"e70006"},"PeriodicalIF":1.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxoplasma gondii (GRA10): A Closer Glance Into Immunogenic and Biochemical Characteristics Using In Silico Approach. 刚地弓形虫（GRA10）的免疫原性和生化特性研究

IF 1.4 4区医学

Parasite Immunology Pub Date : 2025-02-01 DOI: 10.1111/pim.70004

Mahdi Khadem Mohammadi, Ali Dalir Ghaffari

{"title":"Toxoplasma gondii (GRA10): A Closer Glance Into Immunogenic and Biochemical Characteristics Using In Silico Approach.","authors":"Mahdi Khadem Mohammadi, Ali Dalir Ghaffari","doi":"10.1111/pim.70004","DOIUrl":"10.1111/pim.70004","url":null,"abstract":"Toxoplasma gondii, an obligate, intracellular, protozoan parasite, is known to infect a wide range of warm-blooded animals, including livestock, marine mammals and humans leading to the development of toxoplasmosis. The dense granule antigens (GRAs) have garnered crucial role in parasite survival, virulence and the formation of the parasitophorous vacuole. The study focusing on the GRA10 protein of T. gondii aims to elucidate its features further to support its potential inclusion in future vaccine formulations. The present study provided an exhaustive elucidation of the key characteristics of the GRA10 protein, encompassing its presence of a transmembrane domain, physico-chemical properties, subcellular localization, potential epitopes recognised by B-cells and T-cells, secondary and tertiary structures, as well as other significant attributes of this protein. The results indicated that the GRA10 protein possesses a total of 192 possible post-translational modification sites, with no transmembrane domains being detected within its structure. In terms of secondary structure, the GRA10 protein is composed of 27.74% alpha-helix, 13.53% extended strand and 58.72% random coil elements. Additionally, various potential B- and T-cell epitopes were pinpointed for the GRA10 protein, suggesting its immunogenic properties. The assessment of antigenicity and allergenicity further confirmed that GRA10 is immunogenic but non-allergenic, making it a promising candidate for further study. Furthermore, the induction of IFN-γ and IL-4 highlighted the ability of related MHC-II molecules to interact with GRA10, indicating its potential role in immune responses. These findings shed light on the multifaceted nature of the GRA10 protein and its significance in immunological processes. The study presented crucial fundamental and theoretical information regarding GRA10 in order to facilitate the creation of a potent vaccine against both acute and chronic toxoplasmosis, warranting additional in vivo examinations.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":"47 2","pages":"e70004"},"PeriodicalIF":1.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solid Lipid Nanoparticles Encapsulated With Paromomycin: An Effective Oral Formulation Against Leishmania major in Mouse Model. 用帕罗霉素包封的固体脂质纳米颗粒：一种有效的抗小鼠利什曼原虫口服制剂。

IF 1.4 4区医学

Parasite Immunology Pub Date : 2025-02-01 DOI: 10.1111/pim.70002

Maryam Heidari-Kharaji, Priscilla Rodrigues, Christina Petersen

{"title":"Solid Lipid Nanoparticles Encapsulated With Paromomycin: An Effective Oral Formulation Against Leishmania major in Mouse Model.","authors":"Maryam Heidari-Kharaji, Priscilla Rodrigues, Christina Petersen","doi":"10.1111/pim.70002","DOIUrl":"10.1111/pim.70002","url":null,"abstract":"Leishmaniasis is a disease caused by protozoan parasites of the genus Leishmania. The treatment of intracellular infections such as leishmaniasis is often challenging due to limited drug access to infected cells and the development of drug resistance. Therefore, the development of new antileishmanial compounds is essential. Paromomycin sulphate (PM) has shown promise as an antileishmanial drug, and one way to enhance its effectiveness is through appropriate delivery systems. Solid lipid nanoparticles (SLN) are being explored as a potential delivery system for PM, as they offer advantages over other colloidal carriers. In a recent study, PM was loaded into solid lipid nanoparticles (PM-SLN) and its oral effectiveness was evaluated in treating Leishmania (L.) major-infected mice. The study measured footpad swelling, quantified parasite load through real-time PCR and assessed levels of cytokines such as interleukin-4 (IL-4) and gamma interferon (IFN-c), nitric oxide (NO) and Arginase (ARG). Overall, the study demonstrated that oral administration of the PM-SLN formulation is safe and effective in treating leishmaniasis. The SLN in the PM-SLN compound improved the killing of parasites by PM and stimulated a Th1 immune response, indicating its potential as a treatment for leishmaniasis.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":"47 2","pages":"e70002"},"PeriodicalIF":1.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serosurvey of Toxocariasis and Its Association With Allergic Asthma in Children: A Case-Control Study in Southwest Iran. 伊朗西南部儿童弓形虫病血清调查及其与过敏性哮喘的关系：一项病例对照研究

IF 1.4 4区医学

Parasite Immunology Pub Date : 2025-02-01 DOI: 10.1111/pim.70005

Ali Pouryousef, Reza Abbasi, Samer Mehrabi, AbdolAli Moshfe, Fattaneh Mikaeili, Zahra Rezaei, Davoud Rostamzadeh, AminAlah Saadat, Nasir ArefKhah

{"title":"Serosurvey of Toxocariasis and Its Association With Allergic Asthma in Children: A Case-Control Study in Southwest Iran.","authors":"Ali Pouryousef, Reza Abbasi, Samer Mehrabi, AbdolAli Moshfe, Fattaneh Mikaeili, Zahra Rezaei, Davoud Rostamzadeh, AminAlah Saadat, Nasir ArefKhah","doi":"10.1111/pim.70005","DOIUrl":"10.1111/pim.70005","url":null,"abstract":"Evidence from multiple data sources indicates that toxocariasis might play a role in the allergic asthma in children. This case-control study investigated the connection between Toxocara seropositivity and allergic asthma. It also sought to identify the conventional risk factors associated with both asthma and Toxocara infection. In this case-control study, 200 allergic asthma children and 208 children with no history of allergic asthma were included as the healthy group. Sera samples were examined for specific anti-Toxocara IgG antibodies using a serological assay. The overall prevalence of toxocariasis was 6.9% among children. The seroprevalence of toxocariasis in asthmatic children and healthy children was 8% and 5.8%, respectively, with no significant difference (p-value = 0.373). In subgroup analyses based on sociodemographic, Toxocara infection seropositivity was significantly associated with two independent variables, including the father's occupation (p-value = 0.017) and soil contact in asthmatic children (p-value = 0.033). Our results showed a higher prevalence of Toxocara infection in asthmatic children compared to healthy controls, but this difference was not statistically significant. To gain a clearer understanding and comparison of Toxocara infection rates between asthmatic and healthy children, additional comprehensive studies with larger sample sizes and diverse detection methods using different antigenic sources are required.","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":"47 2","pages":"e70005"},"PeriodicalIF":1.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0