Panminerva medica最新文献

{"title":"Microbiota restoration for recurrent Clostridioides difficile infection.","authors":"Sahil Khanna","doi":"10.23736/S0031-0808.24.05111-5","DOIUrl":"10.23736/S0031-0808.24.05111-5","url":null,"abstract":"<p><p>Since the publication of the recent North American and European guidelines on management of Clostridioides difficile infection (CDI), new evidence describing the epidemiology, testing and treatment of CDI has emerged. Despite all advances in infection control and antibiotic stewardship, the incidence and burden of CDI in the hospitals and the community remains at a stable high. Coupled with the incidence of primary CDI, there is a stable high incidence of recurrent CDI. Testing for primary and recurrent CDI remains a clinical challenge owing to high sensitivity of the PCR (leading to false positives) and somewhat limited sensitivity of EIA for toxin. The pathophysiology of recurrent CDI involves an ongoing disruption of the microbiota owing to the infection and the treatment of CDI employed. Broad spectrum antibiotics such as vancomycin leads to further disruption of microbiota compared to fidaxomicin which has a lower disruption of the microbiota and leads to fewer recurrences. Owing to these data fidaxomicin is considered as the first line antibiotic for recurrent CDI. Intravenous bezlotoxumab is a monoclonal antibody that reduces the risk of recurrence in high-risk patients but does not restore the microbiota. Experimental fecal microbiota transplantation (FMT) has been available for more than a decade. Owing to the success of FMT, two new non-invasive donor dependent Food and Drug Administration (FDA) approved therapies have been available since late 2022. This review summarizes all these conundrums regarding CDI and provides clinical pearls to use in day-to-day practice.</p>","PeriodicalId":19851,"journal":{"name":"Panminerva medica","volume":" ","pages":"417-426"},"PeriodicalIF":4.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of different doses of simvastatin on autophagy and inflammation in asthmatic mice.","authors":"Chaowen Huang, Yile Huang, Ming Peng, Xueying Zhong, Jinzhai Tong, Yanming Huang","doi":"10.23736/S0031-0808.23.04922-4","DOIUrl":"10.23736/S0031-0808.23.04922-4","url":null,"abstract":"","PeriodicalId":19851,"journal":{"name":"Panminerva medica","volume":" ","pages":"449-450"},"PeriodicalIF":4.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9918665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition against p38/MEF2C pathway by Pamapimod protects osteoarthritis chondrocytes hypertrophy.","authors":"Jian Zhang, Chen Yan, Weidong He, Min Wang, Jian Liu","doi":"10.23736/S0031-0808.20.04170-1","DOIUrl":"10.23736/S0031-0808.20.04170-1","url":null,"abstract":"<p><strong>Background: </strong>The p38 mitogen-activated protein kinase pathway plays an important role in the pathogenesis of osteoarthritis (OA) involving in hypertrophy, calcification, and apoptosis of chondrocytes (CHs). In this study, we focused on a p38 inhibitor named Pamapimod (PAM) in the effect of CH hypertrophy degeneration.</p><p><strong>Methods: </strong>CHs were isolated from the cartilage collected from OA patients. Insulin-Transferrin-Selenium (ITS) medium was used as a hypertrophic inducer to establish CH hypertrophy model. Asiatic acid (AA) was used to activate p38 phosphorylation. We transfected CHs with myocyte enhancer factor 2C (MEF2C)-plasmid to upregulate MEF2C expression. Chondrogenic gene expression such as type II collagen and SOX-9, and hypertrophic genes such as type X collagen, MMP-13, and Runx-2 were analyzed by western blot, real-time polymerase chain reaction or immunofluorescence.</p><p><strong>Results: </strong>ITS and AA all contributed to the CHs hypertrophy with an upregulation of p-p38 and MEF2C protein expression. PAM treatments significantly inhibited p-p38 and MEF2C expression, down-regulated type X collagen, MMP-13, and Runx-2 expression and upregulated type II collagen and SOX-9 levels. PAM indirectly affected MEF2C expression and resulted in CHs hypertrophy suppression.</p><p><strong>Conclusions: </strong>PAM protects CHs hypertrophy by the inhibition of the p38/MEF2C pathway.</p>","PeriodicalId":19851,"journal":{"name":"Panminerva medica","volume":" ","pages":"365-371"},"PeriodicalIF":4.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38324142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The application research of scenario simulation teaching method in the teaching course of traditional Chinese medicine orthopedics and traumatology.","authors":"Jianmin Li, Dong Zhang, Hao Sun, Ying Yu, Kai Lv, Shuqiang Wang","doi":"10.23736/S0031-0808.24.05057-2","DOIUrl":"10.23736/S0031-0808.24.05057-2","url":null,"abstract":"","PeriodicalId":19851,"journal":{"name":"Panminerva medica","volume":" ","pages":"447-449"},"PeriodicalIF":4.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140922586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative analysis of β-amyloid accumulation in the brain of Alzheimer's disease patients based on PET/CT and its longitudinal correlation with cognitive decline.","authors":"Pan Tang, Ling Zhang, Pingping Li, Chong Cheng, Cong Lin, Bin Tang","doi":"10.23736/S0031-0808.24.05254-6","DOIUrl":"https://doi.org/10.23736/S0031-0808.24.05254-6","url":null,"abstract":"","PeriodicalId":19851,"journal":{"name":"Panminerva medica","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical study on the effect of different administration methods of compound topicamide on pupil dilation in children.","authors":"Haijuan Zhu, Lingling Dang, Ningning Qiao, Xiumei Ma, Mingzhu Lu, Jinbao Wang","doi":"10.23736/S0031-0808.24.05182-6","DOIUrl":"https://doi.org/10.23736/S0031-0808.24.05182-6","url":null,"abstract":"","PeriodicalId":19851,"journal":{"name":"Panminerva medica","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of humanistic care combined with positive psychological intervention on self-efficacy, psychological resilience and QoL in patients with deep vein thrombosis of lower extremities.","authors":"Huibo Li, Junling Wang, Quanyi Wang, Chaoli Guo","doi":"10.23736/S0031-0808.24.05181-4","DOIUrl":"https://doi.org/10.23736/S0031-0808.24.05181-4","url":null,"abstract":"","PeriodicalId":19851,"journal":{"name":"Panminerva medica","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application and research progress of Billroth-II combined with Braun anastomosis in laparoscopic radical gastrectomy of distal gastric cancer.","authors":"Xionghui He, Zhenzhen Li, Wenbo Shi, Jun Liu, Shijun Huang","doi":"10.23736/S0031-0808.24.05180-2","DOIUrl":"https://doi.org/10.23736/S0031-0808.24.05180-2","url":null,"abstract":"","PeriodicalId":19851,"journal":{"name":"Panminerva medica","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Edaravone combined with eurekline on cerebral vascular reserve volume in patients with acute cerebral infarction.","authors":"Xiaoli Wang, Weijian Meng, Xinying Wang, Wenting Ding, Zhi Wang, Yan Wei","doi":"10.23736/S0031-0808.24.05167-X","DOIUrl":"https://doi.org/10.23736/S0031-0808.24.05167-X","url":null,"abstract":"","PeriodicalId":19851,"journal":{"name":"Panminerva medica","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-21-5p reduces apoptosis and inflammation in rats with spinal cord injury through PI3K/AKT pathway.","authors":"Xinwang Lv, Jian Liang, Zhipu Wang","doi":"10.23736/S0031-0808.20.03974-9","DOIUrl":"10.23736/S0031-0808.20.03974-9","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study is to explore the effect of micro ribonucleic acid (miR)-21-5p on spinal cord injury (SCI) in rats and its mechanism of action.</p><p><strong>Methods: </strong>The rat model of SCI was established, and the key miRNAs were screened using the microarray assay and miRNA-mRNA interaction network. After intrathecal injection of agomir-21 and antagomir-21, the effect of miR-21 expression on motor function recovery of rats was evaluated using the Basso-Beattie-Bresnahan (BBB) score. The expression level of miR-21 in spinal cord tissues was determined via quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and the effect of miR-21 expression on apoptosis in spinal cord tissues was determined via terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay and Western blotting. Moreover, the effects of agomir-21 and antagomir-21 on SCI-induced expressions of inflammatory factors interleukin-8 (IL-8), IL-1β, IL-6 and tumor necrosis factor-α (TNF-α) in spinal cord tissues were detected through qRT-PCR. Finally, Western blotting was performed to detect the effects of agomir-21 and antagomir-21 on the phosphatidylinositol 3-hydroxy kinase (PI3K)/protein kinase B (AKT) signaling pathway and its downstream molecules in each group.</p><p><strong>Results: </strong>The screening results of the microarray assay revealed that the mRNA and miRNA expression profiles in spinal cord tissues had significant differences in model group from those in sham group. The BBB score was significantly higher in agomir-21 group than that in model group. Compared with that in model group, the apoptosis of spinal cord tissues was obviously weakened in agomir-21 group, while it was obviously enhanced in antagomir-21 group. Agomir-21 group had evidently lower Bax/Bcl-2, and Caspase-3 and Caspase-9 protein expressions, while antagomir-21 group had evidently higher Bax/Bcl-2 and Caspase-3 protein expression than model group. Besides, the expressions of inflammatory factors IL-8, IL-1β, IL-6 and TNF-α were remarkably lower in agomir-21 group than those in model group, while they were remarkably higher in antagomir-21 group than those in model group. Finally, it was found that the protein expressions of phosphorylated PI3K (p-PI3K)/PI3K and p-AKT/AKT rose markedly, while the protein expressions of phosphatase and tensin homolog deleted on chromosome ten (PTEN) and endothelial nitric oxide synthase (eNOS) declined markedly in agomir-21 group compared with those in model group. However, the opposite results were observed in antagomir-21 group compared with those in model group.</p><p><strong>Conclusions: </strong>MiR-21-5p may reduce the apoptosis and inflammation in spinal cord tissues of rats through the PI3K/AKT pathway.</p>","PeriodicalId":19851,"journal":{"name":"Panminerva medica","volume":" ","pages":"256-265"},"PeriodicalIF":4.3,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38200279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}