Peptide Science最新文献_第5页

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IF 2.4 4区生物学

Peptide Science Pub Date : 2023-07-01 DOI: 10.1002/pep2.24278

引用次数: 0

Development of Convergent Hybrid Phase Ligation for Efficient and Convenient Total Synthesis of Proteins. 开发用于高效便捷地全合成蛋白质的聚合混合相连接技术。

IF 1.5 4区生物学

Peptide Science Pub Date : 2023-07-01 Epub Date: 2023-05-25 DOI: 10.1002/pep2.24323

Ziyong Z Hong, Ruixuan R Yu, Xiaoyu Zhang, Allison M Webb, Nathaniel L Burge, Michael G Poirier, Jennifer J Ottesen

{"title":"Development of Convergent Hybrid Phase Ligation for Efficient and Convenient Total Synthesis of Proteins.","authors":"Ziyong Z Hong, Ruixuan R Yu, Xiaoyu Zhang, Allison M Webb, Nathaniel L Burge, Michael G Poirier, Jennifer J Ottesen","doi":"10.1002/pep2.24323","DOIUrl":"10.1002/pep2.24323","url":null,"abstract":"<p><p>Simple and efficient total synthesis of homogeneous and chemically modified protein samples remains a significant challenge. Here, we report development of a convergent hybrid phase native chemical ligation (CHP-NCL) strategy for facile preparation of proteins. In this strategy, proteins are split into ~100-residue blocks, and each block is assembled on solid support from synthetically accessible peptide fragments before ligated together into full-length protein in solution. With the new method, we increase the yield of CENP-A synthesis by 2.5-fold compared to the previous hybrid phase ligation approach. We further extend the new strategy to the total chemical synthesis of 212-residue linker histone H1.2 in unmodified, phosphorylated, and citrullinated forms, each from eight peptide segments with only one single purification. We demonstrate that fully synthetic H1.2 replicates the binding interactions of linker histones to intact mononucleosomes, as a proxy for the essential function of linker histones in the formation and regulation of higher order chromatin structure.</p>","PeriodicalId":19825,"journal":{"name":"Peptide Science","volume":"115 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10488053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10605700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controlled aggregation properties of threonine modified by protecting groups to unusual self‐assembled structures 保护基修饰苏氨酸的可控聚集特性

IF 2.4 4区生物学

Peptide Science Pub Date : 2023-06-22 DOI: 10.1002/pep2.24324

Nidhi Gour, Vivekshinh Kshtriya, B. Koshti, Monisha Patel, D. Boukhvalov

{"title":"Controlled aggregation properties of threonine modified by protecting groups to unusual self‐assembled structures","authors":"Nidhi Gour, Vivekshinh Kshtriya, B. Koshti, Monisha Patel, D. Boukhvalov","doi":"10.1002/pep2.24324","DOIUrl":"https://doi.org/10.1002/pep2.24324","url":null,"abstract":"We report for the very first time the controlled structural changes in the self‐assemblies of N‐(9‐fluorenylmethoxycarbonyl)‐O‐tert‐butyl‐l‐threonine (Fmoc‐Thr(tBu)‐OH) (FTU) to well defined unique morphologies. The self‐assembling properties of FTU were very interesting and intriguing as it resulted in the formation of unusual structures which resembles fibrous dumbbells and double‐sided broomstick‐like morphologies along with conventional spheres and rods under controlled conditions of concentration and temperature. The self‐assembly of other derivatives of threonine as well as another hydroxyl containing amino acid with same modification that is, ((N‐(9H‐fluoren‐9‐yl)methoxy)carbonyl)‐O‐(tert‐butyl)‐l‐serine (Fmoc‐Ser(tBu)‐OH) (FSU) was also studied to understand the crucial role of –Fmoc, ‐tBu and an additional –CH3 group present in the structure of FTU in the process of self‐assembly. Solvent dependent morphological studies of FTU and FSU suggest important role of solubility parameters and crystallization in formation of these unusual structures. The control experiments of co‐incubation with tannic acid and urea and solution state 1H‐NMR studies elucidate π–π stacking interactions as the key driving force for the structure formation. Further, the interactions which can occur between pairs of FTU and FSU which cause initial self‐assembly was studied theoretically via computational modeling. These studies suggest pair of FTU can either interact via head‐to‐head (HH) or head‐to‐tail (HT) configurations and the most favorable probabilities of either of these interactions lead to morphological transitions in FTU self‐assembly under varying conditions. The studies reported herein hence demonstrate that bioorganic molecules like protected single amino acids can be efficiently used as scaffold for self‐assembly and provide a very simple and facile bottom‐up‐approach for the design of uncommon novel micro/nanoarchitects for multifarious applications.","PeriodicalId":19825,"journal":{"name":"Peptide Science","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45164817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information 问题信息

IF 2.4 4区生物学

Peptide Science Pub Date : 2023-05-01 DOI: 10.1111/nous.12413

引用次数: 0

Nanomole‐scale photochemical thiol‐ene chemistry for high‐throughput late‐stage diversification of peptide macrocycles 纳米级光化学硫醇烯化学用于肽大环的高通量后期多样化

IF 2.4 4区生物学

Peptide Science Pub Date : 2023-04-26 DOI: 10.1002/pep2.24310

M. Nolan, M. Schüttel, Eoin M. Scanlan, Alexander L. Nielsen

引用次数: 0

Erratum: Current peptide vaccine and immunotherapy approaches against Alzheimer's disease 勘误表：目前针对阿尔茨海默病的肽疫苗和免疫疗法方法

IF 2.4 4区生物学

Peptide Science Pub Date : 2023-04-19 DOI: 10.1002/pep2.24309

引用次数: 0

Membrane permeability and antimicrobial peptides: Much more than just making a hole 膜渗透性和抗菌肽:不仅仅是打一个洞

IF 2.4 4区生物学

Peptide Science Pub Date : 2023-03-31 DOI: 10.1002/pep2.24305

J. C. Espeche, Romina Varas, P. Maturana, A. Cutró, P. Maffía, A. Hollmann

引用次数: 1

Host defense peptides identified in human apolipoprotein B as natural food bio‐preservatives: Evaluation of their biosafety and digestibility 人载脂蛋白B中鉴定的宿主防御肽作为天然食品生物防腐剂：生物安全性和消化率评估

IF 2.4 4区生物学

Peptide Science Pub Date : 2023-03-29 DOI: 10.1002/pep2.24308

Eliana Dell’Olmo, Katia Pane, Martina Schibeci, Angela Cesaro, Maria G De Luca, Shurooq Ismail, R. Gaglione, Angela Arciello

引用次数: 0

Fine‐tuning the antimicrobial activity of β‐hairpin peptides with fluorinated amino acids 用氟化氨基酸微调β -发夹肽的抗菌活性

IF 2.4 4区生物学

Peptide Science Pub Date : 2023-03-08 DOI: 10.1002/pep2.24306

S. Chowdhary, Tim Pelzer, Mareike Saathoff, Elisa Quaas, Johanna Pendl, M. Fulde, B. Koksch

引用次数: 1

Guidelines for designing peptoid structures: Insights from the Peptoid Data Bank peptoid结构设计指南：peptoid数据库的见解

IF 2.4 4区生物学

Peptide Science Pub Date : 2023-03-08 DOI: 10.1002/pep2.24307

James R B Eastwood, Ethan I. Weisberg, Dana Katz, R. Zuckermann, K. Kirshenbaum

{"title":"Guidelines for designing peptoid structures: Insights from the Peptoid Data Bank","authors":"James R B Eastwood, Ethan I. Weisberg, Dana Katz, R. Zuckermann, K. Kirshenbaum","doi":"10.1002/pep2.24307","DOIUrl":"https://doi.org/10.1002/pep2.24307","url":null,"abstract":"The number of structural studies of peptoids has grown dramatically over the past 20 years. To date, over 100 high‐resolution structures have been reported for peptoids, which are typically defined as N‐substituted glycine oligomers. We have collected these structures and standardized their sequence representations to facilitate structural analysis as the dataset continues to grow. These structures are presented online as The Peptoid Data Bank (databank.peptoids.org), which also provides persistent links to the published structural data. This review analyzes the present collection of structures and finds extensive support for grouping side chains by their chemistry at the position adjacent to the backbone nitrogen. Groups of side chains with similar chemistry at this position show similar influences on the conformational preferences of the backbone. We also observe a relationship between the side chain and backbone conformations for many monomers that has not previously attracted significant discussion: the values of the χ1 and ϕ dihedrals are correlated. We outline a general design strategy for attaining a specific backbone conformation based on the patterns seen in the collected structures.","PeriodicalId":19825,"journal":{"name":"Peptide Science","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47638341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4